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The map label for this gene is traA [H]

Identifier: 189183302

GI number: 189183302

Start: 413970

End: 415286

Strand: Direct

Name: traA [H]

Synonym: OTT_0395

Alternate gene names: 189183302

Gene position: 413970-415286 (Clockwise)

Preceding gene: 189183300

Following gene: 189183303

Centisome position: 20.61

GC content: 33.26

Gene sequence:

>1317_bases
GTGAGAATTGGTATTGAGATGGCTATACAGTTTACAAGGATTGAATTTTTAACTAGAAGTAAAGGAGGTGATAGTTGTCG
TAAGGCAGCTTATAATGCAAGAACTATTGTTGAAAACGAGAAGACAGGTATAAAGTATAACTTCTCTCGTAAGAAAGATA
ACGTATATCATACAGTGCTGATACCGGATTATATAAATCAAGAATTCAAGAATATTCAAACATTAATGAATGAGGTAGAA
CGAACCGCAAAAAACCAAAAAAACAGCCAGTTGTTGAAGGATATAGTAATAGCATTGCCAGACGAGAAGGAGCTAAATTT
AGAGCATAGAATAGAAATAACTCATCAAATAGTTGATGCGATGGAATGGGTGCAAAATGGTCTTGGAGTACAGATAGATA
TTCATAAGCCTCATACAGGAGATAAAAACTGGCATGCACATATATTGCTTACTATAAGAAGATTTAGAGAAGATGGAACT
GGTTTAGGAGATATAGCAGTAGATTTAAACCCAAAAATTATAACATTAAGTAATGGCAAAAAGGTTGTTATTAAAGATCC
AGAGATGATTCATGAAATAGTAAAAGACATAATTAATGCATATTTCGCTAAATTAGGCTTACCATATAGAGTTGACGAGA
TAAGCGAAGTGCCTGGAGAGCATATGGGGCGTATTAAAATTAGGAGTTTAATTAATAAAGTAGTAAATGAAAATGAGTTA
CGTAAAGAGGCTCATTTGAAGATTATTAATGATGCTGACGTAATAACAGATTCTATAACACATTACAAATCTATTTTTAC
TAAGCAGGATATTGAAAAAGCAGTAAAAGATATACCAGATCTAACAGCAAGAGAACAGTTAGTTCAGCAAGTGCTTAGTT
CAAATAGAATACTAGAGTTATACCATGATGATGGTGAAAGTAGCAAATATTTTACGACAATTGAGGTTCGAAATGAGGAG
ACGAGAATAATCAGAATAGCTAATAAAATCAATGATCAGGTTTATTACAACGATATTTACAATCTTAAAAGTGATATCGA
AGGTCTAGCAAATGTTAGTGAAGAACAGAAACAAGCTCTAAGGCATATTTTGCTTAGCACTAGTGGAGTTAGAGTCTTGA
GAGGAAGAGCTGGTACAGGAAAATCTTATGTTTTAATAAAAGCGCATAAGCTCGCAACAAATCGTAGACAAAAAGTTATT
GGTCTTGCTCCTACTCATAAGGCAGTATCAGAGCTGAGGAGCAAAGGTTATACAGAGGTCTATACAGTAAAAGGATTTTT
GTATAATCGAAAAAAAATTTTATGCAAGGCAGCTTAA

Upstream 100 bases:

>100_bases
ATTGCTGCTAAAAAATGAGTACTCATTAACCGATTGAATGTTAAAAAAAAAGAAGTTTATGCTAGCAAAATCAAAAAAAC
CATGCTAACTTAAAACTCAG

Downstream 100 bases:

>100_bases
TAGTAGTAGATGAAGCTGGAATGGTAGGTACTAAAGCTTATGCAGAGCTGTTTAGAGTAGTTAGAAACAATAATTGTCAA
CTGATACTTGCTGGAGATGA

Product: putative conjugative transfer protein TraA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MRIGIEMAIQFTRIEFLTRSKGGDSCRKAAYNARTIVENEKTGIKYNFSRKKDNVYHTVLIPDYINQEFKNIQTLMNEVE
RTAKNQKNSQLLKDIVIALPDEKELNLEHRIEITHQIVDAMEWVQNGLGVQIDIHKPHTGDKNWHAHILLTIRRFREDGT
GLGDIAVDLNPKIITLSNGKKVVIKDPEMIHEIVKDIINAYFAKLGLPYRVDEISEVPGEHMGRIKIRSLINKVVNENEL
RKEAHLKIINDADVITDSITHYKSIFTKQDIEKAVKDIPDLTAREQLVQQVLSSNRILELYHDDGESSKYFTTIEVRNEE
TRIIRIANKINDQVYYNDIYNLKSDIEGLANVSEEQKQALRHILLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRRQKVI
GLAPTHKAVSELRSKGYTEVYTVKGFLYNRKKILCKAA

Sequences:

>Translated_438_residues
MRIGIEMAIQFTRIEFLTRSKGGDSCRKAAYNARTIVENEKTGIKYNFSRKKDNVYHTVLIPDYINQEFKNIQTLMNEVE
RTAKNQKNSQLLKDIVIALPDEKELNLEHRIEITHQIVDAMEWVQNGLGVQIDIHKPHTGDKNWHAHILLTIRRFREDGT
GLGDIAVDLNPKIITLSNGKKVVIKDPEMIHEIVKDIINAYFAKLGLPYRVDEISEVPGEHMGRIKIRSLINKVVNENEL
RKEAHLKIINDADVITDSITHYKSIFTKQDIEKAVKDIPDLTAREQLVQQVLSSNRILELYHDDGESSKYFTTIEVRNEE
TRIIRIANKINDQVYYNDIYNLKSDIEGLANVSEEQKQALRHILLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRRQKVI
GLAPTHKAVSELRSKGYTEVYTVKGFLYNRKKILCKAA
>Mature_438_residues
MRIGIEMAIQFTRIEFLTRSKGGDSCRKAAYNARTIVENEKTGIKYNFSRKKDNVYHTVLIPDYINQEFKNIQTLMNEVE
RTAKNQKNSQLLKDIVIALPDEKELNLEHRIEITHQIVDAMEWVQNGLGVQIDIHKPHTGDKNWHAHILLTIRRFREDGT
GLGDIAVDLNPKIITLSNGKKVVIKDPEMIHEIVKDIINAYFAKLGLPYRVDEISEVPGEHMGRIKIRSLINKVVNENEL
RKEAHLKIINDADVITDSITHYKSIFTKQDIEKAVKDIPDLTAREQLVQQVLSSNRILELYHDDGESSKYFTTIEVRNEE
TRIIRIANKINDQVYYNDIYNLKSDIEGLANVSEEQKQALRHILLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRRQKVI
GLAPTHKAVSELRSKGYTEVYTVKGFLYNRKKILCKAA

Specific function: traI Has Been Identified As DNA Helicase I And It Also Has An Additional Activity Of Site-Specific Nicking At Orit. DNA Helicase I Is A Potent DNA-Dependent ATPase. [C]

COG id: COG0507

COG function: function code L; ATP-dependent exoDNAse (exonuclease V), alpha subunit - helicase superfamily I member

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mobA/mobL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000606
- InterPro:   IPR005053
- InterPro:   IPR014136 [H]

Pfam domain/function: PF03389 MobA_MobL; PF01443 Viral_helicase1 [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 50214; Mature: 50214

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIEMAIQFTRIEFLTRSKGGDSCRKAAYNARTIVENEKTGIKYNFSRKKDNVYHTVL
CCCCHHHHEEHHHHHHHHCCCCCCHHHHHHCCCHHEEECCCCCEEEEECCCCCCEEEEEE
IPDYINQEFKNIQTLMNEVERTAKNQKNSQLLKDIVIALPDEKELNLEHRIEITHQIVDA
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
MEWVQNGLGVQIDIHKPHTGDKNWHAHILLTIRRFREDGTGLGDIAVDLNPKIITLSNGK
HHHHHCCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHCCCCCCEEEEECCCEEEEECCCC
KVVIKDPEMIHEIVKDIINAYFAKLGLPYRVDEISEVPGEHMGRIKIRSLINKVVNENEL
EEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHCHHHHHHHHHHHHCHHHH
RKEAHLKIINDADVITDSITHYKSIFTKQDIEKAVKDIPDLTAREQLVQQVLSSNRILEL
HHHHCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEE
YHDDGESSKYFTTIEVRNEETRIIRIANKINDQVYYNDIYNLKSDIEGLANVSEEQKQAL
EECCCCCCEEEEEEEEECCCCEEEEEEHHHCCCEEEHHHHHHHHHHHHHHCCCHHHHHHH
RHILLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRRQKVIGLAPTHKAVSELRSKGYTEV
HHHHHCCCCCEEEECCCCCCCEEEEEEEHHHHCCCHHEEEEECCCHHHHHHHHHCCCCEE
YTVKGFLYNRKKILCKAA
EEEEHEEECCCEEEEECC
>Mature Secondary Structure
MRIGIEMAIQFTRIEFLTRSKGGDSCRKAAYNARTIVENEKTGIKYNFSRKKDNVYHTVL
CCCCHHHHEEHHHHHHHHCCCCCCHHHHHHCCCHHEEECCCCCEEEEECCCCCCEEEEEE
IPDYINQEFKNIQTLMNEVERTAKNQKNSQLLKDIVIALPDEKELNLEHRIEITHQIVDA
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
MEWVQNGLGVQIDIHKPHTGDKNWHAHILLTIRRFREDGTGLGDIAVDLNPKIITLSNGK
HHHHHCCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHCCCCCCEEEEECCCEEEEECCCC
KVVIKDPEMIHEIVKDIINAYFAKLGLPYRVDEISEVPGEHMGRIKIRSLINKVVNENEL
EEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHCHHHHHHHHHHHHCHHHH
RKEAHLKIINDADVITDSITHYKSIFTKQDIEKAVKDIPDLTAREQLVQQVLSSNRILEL
HHHHCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEE
YHDDGESSKYFTTIEVRNEETRIIRIANKINDQVYYNDIYNLKSDIEGLANVSEEQKQAL
EECCCCCCEEEEEEEEECCCCEEEEEEHHHCCCEEEHHHHHHHHHHHHHHCCCHHHHHHH
RHILLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRRQKVIGLAPTHKAVSELRSKGYTEV
HHHHHCCCCCEEEECCCCCCCEEEEEEEHHHHCCCHHEEEEECCCHHHHHHHHHCCCCEE
YTVKGFLYNRKKILCKAA
EEEEHEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on acid anhydrides; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763954 [H]