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The map label for this gene is eno

Identifier: 189026041

GI number: 189026041

Start: 887038

End: 888336

Strand: Reverse

Name: eno

Synonym: TPASS_0817

Alternate gene names: 189026041

Gene position: 888336-887038 (Counterclockwise)

Preceding gene: 189026042

Following gene: 189026040

Centisome position: 77.96

GC content: 52.35

Gene sequence:

>1299_bases
GTGAGTGACATTGCATGCATTGAAGCGCGCGAGATCATCGATTCTCGGGGAAACCCCACTGTCGAAGTGGATGTCTCGCT
CAGTGACGGTAGCTTTGGCCGTGCGTGTGTTCCTTCAGGGGCGTCTACCGGAGAGTTCGAGGCGCTCGAAATGCGTGATG
GAGACAAGGAGCGCTATAACGGTAAGGGTGTTCTCAAGGCCGTTGGAACTGTCAACACGCTCATTGCTGATACGCTCGAA
GGTATGGATGCGCTCAACCAGGGTGAGATAGATCACGCGATGCGGAATCTGGACGGCACTGACAATAAGTCAAAGCTTGG
TGCGAATGCTATGCTTGGCGTCTCCATGGCTTGTGCGCGTGCTGCTGCAGACTTTCTGGGTGTTCCTCTCTACCGTTACC
TCGGTGGTGTGCACACTTTTCGCATGCCGGTCCCTATGGCCAACATTATCAATGGGGGGAAGCATTCTGATAATAAAATT
GACTTTCAAGAGTTTATGGTTATGCCCATCGGCGCTGCGTCCATGCGCGAAGCGGTGCGCATGACCGCAGAGGTGTTCCA
CGCGTTAAAGGGCCTGCTTGCCGCCGACGGCAAAGCTACGTCAGTTGGTGATGAAGGTGGTTTTGCGCCTGATTTGGATA
ACGAGCAAGCCCTTGAGTACATTATGAAAGCGATCGCAAAAGCCGGCCTTGCGCCGCGGAAGGATGTGTGTATTGCGCTT
GACTGTGCGTCTTCTGAGTTATTCGATGAAGGAGATCGGAGAGGGTACAAGTTTTGGAAGTCGAATCCGGGGAAGCTGTT
CACAGCACAGGAAATGATTGATCTGTACAAGAAGTGGATTGCTACGTATCCGATTGTGTCTATCGAGGATCCGCTTGACC
AAAACGACTGGGCAGGTTACGTGCAGCTCACGAAAGAGTTGGGAGACAAAGTCCAAATTGTGGGAGATGATTTTTTTGTA
ACCAACACTGGGCGTCTGGCCCGAGGTATCAAAGAAGGATCGTGCAATTCGATTCTGATAAAACTTAATCAGATTGGCAC
GGTCACAGAAACTGTCGACGCGGTGCGTATGGCTCAGAACGCAGGCTACGCGGCGGTGATTTCACATCGCTCGGGTGAGA
CAGAGGATGCGTTTATCGCAGACTTAGCAGTGGCACTTGAGACAGGTCAAATCAAAACCGGCTCGATGAGTCGCAGTGAC
CGGGTGGCGAAGTATAACCAGTTGATGCGCATTGAGGAAGAGTTGGGTGCACAGGCGCGCTACTACGGGGCAAAGACCTT
TGAGCGGTTTGGTTGTTAG

Upstream 100 bases:

>100_bases
GTGGGAAGGGAGCGCGGCGTGCGGGGGCGATAGAGAGAGTGGAAAACCTTTACGCATTTTTTTTCGCGTGTTACCATGCG
CCCACATCAGGAGGTCTTAA

Downstream 100 bases:

>100_bases
CTTGTAAGGTGCGAGGAACTCTATAAGAAGTTGGGGTACGGGGCAGAGAACGCAGGACTCCGTGTCGTTTCTTCTTTACT
TCCTGGCGGAATGGTTGCTG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSDIACIEAREIIDSRGNPTVEVDVSLSDGSFGRACVPSGASTGEFEALEMRDGDKERYNGKGVLKAVGTVNTLIADTLE
GMDALNQGEIDHAMRNLDGTDNKSKLGANAMLGVSMACARAAADFLGVPLYRYLGGVHTFRMPVPMANIINGGKHSDNKI
DFQEFMVMPIGAASMREAVRMTAEVFHALKGLLAADGKATSVGDEGGFAPDLDNEQALEYIMKAIAKAGLAPRKDVCIAL
DCASSELFDEGDRRGYKFWKSNPGKLFTAQEMIDLYKKWIATYPIVSIEDPLDQNDWAGYVQLTKELGDKVQIVGDDFFV
TNTGRLARGIKEGSCNSILIKLNQIGTVTETVDAVRMAQNAGYAAVISHRSGETEDAFIADLAVALETGQIKTGSMSRSD
RVAKYNQLMRIEEELGAQARYYGAKTFERFGC

Sequences:

>Translated_432_residues
MSDIACIEAREIIDSRGNPTVEVDVSLSDGSFGRACVPSGASTGEFEALEMRDGDKERYNGKGVLKAVGTVNTLIADTLE
GMDALNQGEIDHAMRNLDGTDNKSKLGANAMLGVSMACARAAADFLGVPLYRYLGGVHTFRMPVPMANIINGGKHSDNKI
DFQEFMVMPIGAASMREAVRMTAEVFHALKGLLAADGKATSVGDEGGFAPDLDNEQALEYIMKAIAKAGLAPRKDVCIAL
DCASSELFDEGDRRGYKFWKSNPGKLFTAQEMIDLYKKWIATYPIVSIEDPLDQNDWAGYVQLTKELGDKVQIVGDDFFV
TNTGRLARGIKEGSCNSILIKLNQIGTVTETVDAVRMAQNAGYAAVISHRSGETEDAFIADLAVALETGQIKTGSMSRSD
RVAKYNQLMRIEEELGAQARYYGAKTFERFGC
>Mature_431_residues
SDIACIEAREIIDSRGNPTVEVDVSLSDGSFGRACVPSGASTGEFEALEMRDGDKERYNGKGVLKAVGTVNTLIADTLEG
MDALNQGEIDHAMRNLDGTDNKSKLGANAMLGVSMACARAAADFLGVPLYRYLGGVHTFRMPVPMANIINGGKHSDNKID
FQEFMVMPIGAASMREAVRMTAEVFHALKGLLAADGKATSVGDEGGFAPDLDNEQALEYIMKAIAKAGLAPRKDVCIALD
CASSELFDEGDRRGYKFWKSNPGKLFTAQEMIDLYKKWIATYPIVSIEDPLDQNDWAGYVQLTKELGDKVQIVGDDFFVT
NTGRLARGIKEGSCNSILIKLNQIGTVTETVDAVRMAQNAGYAAVISHRSGETEDAFIADLAVALETGQIKTGSMSRSDR
VAKYNQLMRIEEELGAQARYYGAKTFERFGC

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=428, Percent_Identity=53.2710280373832, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI4503571, Length=428, Percent_Identity=53.2710280373832, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI301897477, Length=427, Percent_Identity=53.3957845433255, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI301897469, Length=427, Percent_Identity=53.3957845433255, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI301897479, Length=425, Percent_Identity=49.4117647058824, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI169201331, Length=339, Percent_Identity=27.7286135693215, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI169201757, Length=339, Percent_Identity=27.7286135693215, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI239744207, Length=339, Percent_Identity=27.7286135693215, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1789141, Length=431, Percent_Identity=58.0046403712297, Blast_Score=469, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=50.8158508158508, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17536383, Length=429, Percent_Identity=50.8158508158508, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32563855, Length=197, Percent_Identity=47.7157360406091, Blast_Score=189, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=47.6958525345622, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=47.2350230414747, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=47.2350230414747, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321693, Length=431, Percent_Identity=48.0278422273782, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6321968, Length=431, Percent_Identity=48.2598607888631, Blast_Score=352, Evalue=7e-98,
Organism=Drosophila melanogaster, GI24580918, Length=435, Percent_Identity=50.8045977011494, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580916, Length=435, Percent_Identity=50.8045977011494, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580920, Length=435, Percent_Identity=50.8045977011494, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580914, Length=435, Percent_Identity=50.8045977011494, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI281360527, Length=435, Percent_Identity=50.8045977011494, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI17137654, Length=435, Percent_Identity=50.8045977011494, Blast_Score=400, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_TREPA (P74934)

Other databases:

- EMBL:   U61534
- EMBL:   AE000520
- PIR:   F71278
- RefSeq:   NP_219253.1
- ProteinModelPortal:   P74934
- SMR:   P74934
- GeneID:   2610905
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0817
- NMPDR:   fig|243276.1.peg.812
- TIGR:   TP_0817
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   TPAL243276:TP_0817-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46772; Mature: 46640

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 341-341 BINDING 155-155 BINDING 164-164 BINDING 289-289 BINDING 316-316 BINDING 341-341 BINDING 392-392

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIACIEAREIIDSRGNPTVEVDVSLSDGSFGRACVPSGASTGEFEALEMRDGDKERYN
CCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCC
GKGVLKAVGTVNTLIADTLEGMDALNQGEIDHAMRNLDGTDNKSKLGANAMLGVSMACAR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHCCCHHHHHHHHHHHH
AAADFLGVPLYRYLGGVHTFRMPVPMANIINGGKHSDNKIDFQEFMVMPIGAASMREAVR
HHHHHHCCHHHHHHCCCEEEECCCCHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
MTAEVFHALKGLLAADGKATSVGDEGGFAPDLDNEQALEYIMKAIAKAGLAPRKDVCIAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
DCASSELFDEGDRRGYKFWKSNPGKLFTAQEMIDLYKKWIATYPIVSIEDPLDQNDWAGY
ECCCCHHHCCCCCCCCHHHCCCCCCEEEHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHH
VQLTKELGDKVQIVGDDFFVTNTGRLARGIKEGSCNSILIKLNQIGTVTETVDAVRMAQN
HHHHHHCCCEEEEEECCEEEECCHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHC
AGYAAVISHRSGETEDAFIADLAVALETGQIKTGSMSRSDRVAKYNQLMRIEEELGAQAR
CCEEEEEECCCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCHHH
YYGAKTFERFGC
HHCHHHHHHHCC
>Mature Secondary Structure 
SDIACIEAREIIDSRGNPTVEVDVSLSDGSFGRACVPSGASTGEFEALEMRDGDKERYN
CCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCC
GKGVLKAVGTVNTLIADTLEGMDALNQGEIDHAMRNLDGTDNKSKLGANAMLGVSMACAR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHCCCHHHHHHHHHHHH
AAADFLGVPLYRYLGGVHTFRMPVPMANIINGGKHSDNKIDFQEFMVMPIGAASMREAVR
HHHHHHCCHHHHHHCCCEEEECCCCHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
MTAEVFHALKGLLAADGKATSVGDEGGFAPDLDNEQALEYIMKAIAKAGLAPRKDVCIAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
DCASSELFDEGDRRGYKFWKSNPGKLFTAQEMIDLYKKWIATYPIVSIEDPLDQNDWAGY
ECCCCHHHCCCCCCCCHHHCCCCCCEEEHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHH
VQLTKELGDKVQIVGDDFFVTNTGRLARGIKEGSCNSILIKLNQIGTVTETVDAVRMAQN
HHHHHHCCCEEEEEECCEEEECCHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHC
AGYAAVISHRSGETEDAFIADLAVALETGQIKTGSMSRSDRVAKYNQLMRIEEELGAQAR
CCEEEEEECCCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCHHH
YYGAKTFERFGC
HHCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9255517; 9665876