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The map label for this gene is dapF

Identifier: 188586527

GI number: 188586527

Start: 2008724

End: 2009623

Strand: Reverse

Name: dapF

Synonym: Nther_1913

Alternate gene names: 188586527

Gene position: 2009623-2008724 (Counterclockwise)

Preceding gene: 188586528

Following gene: 188586525

Centisome position: 63.48

GC content: 38.44

Gene sequence:

>900_bases
GTGAAGTTTGAAAAAATGCATGGTTTGGGGAACGATTTTATTTTGATGAAAGATTTTCAGAGAAATTATCCTGATCTGGC
AAAACGGCTATGTGACCGTCGGTTTGGAATTGGGGCTGATGGGCTAGTCATTTACTCTTCTTGTAAGAATTCAAACATAA
ATGCTGACTATCAAATGAGAATTTATAATTCTGATGGCTCAGAGGCAGAAATGTGTGGGAATGCTATTCGATGCTTAGCT
AAATATCTGGCAAAAACAGAAGAGACAGATGGAAAACGATTGGCGATTGCAACTACTGCGGGAATCAAAAGAGTTGAAAT
TGTTGAAAATTTTGACTTAATGGGTGAAATTAATTTATCGGGTATGGTTGGAAGTCAAGAACAGCTTGTTAAAGTTAATA
TGGAAAAACCCCTTTTGAAGAGTTCTGATATACCCTTAGCCGTTAAAAACGATAGAAATGACATTGCTCGCCAACGGCCG
ATAGATACACCTTATGGGATATATTATCTGACTGAAGTTTCTACAGGTCCTCCTCATGCCGTAATTTTTGTTCAAAATTT
ACTTCCAGAAGAACAACTATTAGAGTTAGGCCCTATTATTGAAAAGCACGATCTATTCCCCTATGGTACTAACGTGGAGT
TTGTGAAAGTGGAAAGCTCGAACCACTTACGAGTACAAGTTTGGGAAAGAGGAGCAGGTAAAACTTTGGCTTGTGGTACA
GGGGCCTGTGCGGTAGTAGTAGCAGCAACTATTAATGGTTTTTGTAATGGGATGGCATATGTGGAACTGCCCGGTGGCAA
GCTTTTTATCAACTGGGATAATAATACTGAAGAACTTTATATGATTGGCCCAGCTGAGCATGTGTATAGCGGGGAATTAA
ATAATCAAATATTTTATTAA

Upstream 100 bases:

>100_bases
TTAGTAAACCAAGGTCAAGCTGATCTCATAGTTCGTCGAGAAAGTTTAGAAGATGTGATCCAAAATGACCTTATCCCAGA
AAGGTTTAGGAGGAACTAAA

Downstream 100 bases:

>100_bases
TTTAGCAAAAAATAATTTTAAATTTATCTAATTTAGATGATATTACAACAAGTCCGGTCAAGAGCCGGACTATTATTTTT
ATATTACCAAACTTTTCTGT

Product: Diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKFEKMHGLGNDFILMKDFQRNYPDLAKRLCDRRFGIGADGLVIYSSCKNSNINADYQMRIYNSDGSEAEMCGNAIRCLA
KYLAKTEETDGKRLAIATTAGIKRVEIVENFDLMGEINLSGMVGSQEQLVKVNMEKPLLKSSDIPLAVKNDRNDIARQRP
IDTPYGIYYLTEVSTGPPHAVIFVQNLLPEEQLLELGPIIEKHDLFPYGTNVEFVKVESSNHLRVQVWERGAGKTLACGT
GACAVVVAATINGFCNGMAYVELPGGKLFINWDNNTEELYMIGPAEHVYSGELNNQIFY

Sequences:

>Translated_299_residues
MKFEKMHGLGNDFILMKDFQRNYPDLAKRLCDRRFGIGADGLVIYSSCKNSNINADYQMRIYNSDGSEAEMCGNAIRCLA
KYLAKTEETDGKRLAIATTAGIKRVEIVENFDLMGEINLSGMVGSQEQLVKVNMEKPLLKSSDIPLAVKNDRNDIARQRP
IDTPYGIYYLTEVSTGPPHAVIFVQNLLPEEQLLELGPIIEKHDLFPYGTNVEFVKVESSNHLRVQVWERGAGKTLACGT
GACAVVVAATINGFCNGMAYVELPGGKLFINWDNNTEELYMIGPAEHVYSGELNNQIFY
>Mature_299_residues
MKFEKMHGLGNDFILMKDFQRNYPDLAKRLCDRRFGIGADGLVIYSSCKNSNINADYQMRIYNSDGSEAEMCGNAIRCLA
KYLAKTEETDGKRLAIATTAGIKRVEIVENFDLMGEINLSGMVGSQEQLVKVNMEKPLLKSSDIPLAVKNDRNDIARQRP
IDTPYGIYYLTEVSTGPPHAVIFVQNLLPEEQLLELGPIIEKHDLFPYGTNVEFVKVESSNHLRVQVWERGAGKTLACGT
GACAVVVAATINGFCNGMAYVELPGGKLFINWDNNTEELYMIGPAEHVYSGELNNQIFY

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=295, Percent_Identity=36.271186440678, Blast_Score=186, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_NATTJ (B2A663)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001918072.1
- ProteinModelPortal:   B2A663
- SMR:   B2A663
- GeneID:   6315293
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1913
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 33321; Mature: 33321

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 72-72 ACT_SITE 238-238

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFEKMHGLGNDFILMKDFQRNYPDLAKRLCDRRFGIGADGLVIYSSCKNSNINADYQMR
CCCHHHCCCCCCEEEEEHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEE
IYNSDGSEAEMCGNAIRCLAKYLAKTEETDGKRLAIATTAGIKRVEIVENFDLMGEINLS
EECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEECCCEEEEEEEC
GMVGSQEQLVKVNMEKPLLKSSDIPLAVKNDRNDIARQRPIDTPYGIYYLTEVSTGPPHA
CCCCCCCCEEEECCCCCCCCCCCCCEEEECCCHHHHHHCCCCCCCCEEEEEEECCCCCEE
VIFVQNLLPEEQLLELGPIIEKHDLFPYGTNVEFVKVESSNHLRVQVWERGAGKTLACGT
EEEEEHHCCHHHHHHHCCCHHHCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCEEEECC
GACAVVVAATINGFCNGMAYVELPGGKLFINWDNNTEELYMIGPAEHVYSGELNNQIFY
CHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCCCCEEEEECCHHHEECCCCCCCCCC
>Mature Secondary Structure
MKFEKMHGLGNDFILMKDFQRNYPDLAKRLCDRRFGIGADGLVIYSSCKNSNINADYQMR
CCCHHHCCCCCCEEEEEHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEE
IYNSDGSEAEMCGNAIRCLAKYLAKTEETDGKRLAIATTAGIKRVEIVENFDLMGEINLS
EECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEECCCEEEEEEEC
GMVGSQEQLVKVNMEKPLLKSSDIPLAVKNDRNDIARQRPIDTPYGIYYLTEVSTGPPHA
CCCCCCCCEEEECCCCCCCCCCCCCEEEECCCHHHHHHCCCCCCCCEEEEEEECCCCCEE
VIFVQNLLPEEQLLELGPIIEKHDLFPYGTNVEFVKVESSNHLRVQVWERGAGKTLACGT
EEEEEHHCCHHHHHHHCCCHHHCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCEEEECC
GACAVVVAATINGFCNGMAYVELPGGKLFINWDNNTEELYMIGPAEHVYSGELNNQIFY
CHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCCCCEEEEECCHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA