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The map label for this gene is yjbJ [H]

Identifier: 188586502

GI number: 188586502

Start: 1980010

End: 1980597

Strand: Reverse

Name: yjbJ [H]

Synonym: Nther_1888

Alternate gene names: 188586502

Gene position: 1980597-1980010 (Counterclockwise)

Preceding gene: 188586503

Following gene: 188586499

Centisome position: 62.57

GC content: 32.65

Gene sequence:

>588_bases
ATGAATTTGCAAAATTCACGTAAAAGATATTTAATTGCGTTAGTTATAATAATTATAACAGCTTTGTTAACATATTGGTT
TGCAATGCCCAGAATTTGGGAATATCTGTATCCTAAACATTTTCAAGAGGAAGTTTATTCTATAGCTGAAGATCATGAAT
TAGATCCTTATTTACTATTTGCAATTATAAAAGTTGAAAGTAAGTTTGATGAAAAAGCTATTTCAAATCAAGGGGCCATG
GGATTAATGCAGCTTATGCCTACAACTGGAGCTTGGGCTGCTCAAAATGTCACTTATAACTCCTTTGAACATGATGACTT
GTTTGATCCTAAAACTAATATTGAATTAGGAAGCTGGTATTTAGATTACTTGCTATCAGAATTTGATCAAGATTTAGTAG
TCACTCTAGCTGCTTATAATGCAGGACAGGGTAACGTTCGTCAATGGATGGAAATGAATATATGGGATGGCAGCTATGAA
GAATTAGATAATGTACCTTACAATGAAACTAAACATTATGTAAAAAATGTGCTTAGAGATTACGAACGCTATCGCGAAAT
ATACGAAGAATCTTATGCCGGAGAATAA

Upstream 100 bases:

>100_bases
GAATTGCAGGTCAAGCAGATTTATTCAAAATTGCTATAGTAAATCGAGATAGTTTGTAATACAATATGAACAGAAATCGA
TATTAGAGGTGTCTTTAATA

Downstream 100 bases:

>100_bases
ACTTGACAGCTAGGTTGAGTCAATTGTATAATAATTGAGGCTTGTATATATATTATATGTGTATTTAATCTTTAAATTAT
CTGTGCGGAAGTGGTGGAAC

Product: Lytic transglycosylase catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MNLQNSRKRYLIALVIIIITALLTYWFAMPRIWEYLYPKHFQEEVYSIAEDHELDPYLLFAIIKVESKFDEKAISNQGAM
GLMQLMPTTGAWAAQNVTYNSFEHDDLFDPKTNIELGSWYLDYLLSEFDQDLVVTLAAYNAGQGNVRQWMEMNIWDGSYE
ELDNVPYNETKHYVKNVLRDYERYREIYEESYAGE

Sequences:

>Translated_195_residues
MNLQNSRKRYLIALVIIIITALLTYWFAMPRIWEYLYPKHFQEEVYSIAEDHELDPYLLFAIIKVESKFDEKAISNQGAM
GLMQLMPTTGAWAAQNVTYNSFEHDDLFDPKTNIELGSWYLDYLLSEFDQDLVVTLAAYNAGQGNVRQWMEMNIWDGSYE
ELDNVPYNETKHYVKNVLRDYERYREIYEESYAGE
>Mature_195_residues
MNLQNSRKRYLIALVIIIITALLTYWFAMPRIWEYLYPKHFQEEVYSIAEDHELDPYLLFAIIKVESKFDEKAISNQGAM
GLMQLMPTTGAWAAQNVTYNSFEHDDLFDPKTNIELGSWYLDYLLSEFDQDLVVTLAAYNAGQGNVRQWMEMNIWDGSYE
ELDNVPYNETKHYVKNVLRDYERYREIYEESYAGE

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=155, Percent_Identity=32.258064516129, Blast_Score=87, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 23106; Mature: 23106

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQNSRKRYLIALVIIIITALLTYWFAMPRIWEYLYPKHFQEEVYSIAEDHELDPYLLF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHH
AIIKVESKFDEKAISNQGAMGLMQLMPTTGAWAAQNVTYNSFEHDDLFDPKTNIELGSWY
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHH
LDYLLSEFDQDLVVTLAAYNAGQGNVRQWMEMNIWDGSYEELDNVPYNETKHYVKNVLRD
HHHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHH
YERYREIYEESYAGE
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLQNSRKRYLIALVIIIITALLTYWFAMPRIWEYLYPKHFQEEVYSIAEDHELDPYLLF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHH
AIIKVESKFDEKAISNQGAMGLMQLMPTTGAWAAQNVTYNSFEHDDLFDPKTNIELGSWY
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHH
LDYLLSEFDQDLVVTLAAYNAGQGNVRQWMEMNIWDGSYEELDNVPYNETKHYVKNVLRD
HHHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHH
YERYREIYEESYAGE
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]