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The map label for this gene is sigK [H]

Identifier: 188586385

GI number: 188586385

Start: 1837025

End: 1837369

Strand: Reverse

Name: sigK [H]

Synonym: Nther_1770

Alternate gene names: 188586385

Gene position: 1837369-1837025 (Counterclockwise)

Preceding gene: 188586386

Following gene: 188586383

Centisome position: 58.04

GC content: 32.17

Gene sequence:

>345_bases
ATGATACCTGTGATTGGAGTTATATCATTAATTGTCAAAGAAATTTCTTTACTTGTTTCTTATATTAGCAGCAACTCATT
TCCTCAACCTTTATCTGCAGAAGATGAACGCAAATACCTTAAAAGATTAGAACAAGGTGATGATAAAGCTAGGGAAGTTT
TAATTGAAAGAAATCTACGACTAGTAGCCCATGTTATCAAAAAATTTGAAAACAGTGGTGAAGACCCAGAAGATTTAATT
TCAATTGGTACAATAGGATTAATCAAAGCGATTAACACTTATAATTATAGAAAGGGTACTAGATTAGCCACTTACGCTGC
ACGATGCATTGAAAATGAGAGTTAA

Upstream 100 bases:

>100_bases
ATTTTAATAATATTTCTTAAGACAGGCACGAATTTTAGATAAGACCATATAATAATTGTACCCCTTGCATTTCTTATACT
AGAGGAGGTGTTATATTTTA

Downstream 100 bases:

>100_bases
CTTACAATACTGTTGCGGAAAAATATGGTATTTAAAATTTTTGAATTAATTACTACATTGAAATGGATACTAAGATTATA
ATTCCAATAAAGATAACATA

Product: RNA polymerase, sigma 28 subunit, FliA/WhiG family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 114; Mature: 114

Protein sequence:

>114_residues
MIPVIGVISLIVKEISLLVSYISSNSFPQPLSAEDERKYLKRLEQGDDKAREVLIERNLRLVAHVIKKFENSGEDPEDLI
SIGTIGLIKAINTYNYRKGTRLATYAARCIENES

Sequences:

>Translated_114_residues
MIPVIGVISLIVKEISLLVSYISSNSFPQPLSAEDERKYLKRLEQGDDKAREVLIERNLRLVAHVIKKFENSGEDPEDLI
SIGTIGLIKAINTYNYRKGTRLATYAARCIENES
>Mature_114_residues
MIPVIGVISLIVKEISLLVSYISSNSFPQPLSAEDERKYLKRLEQGDDKAREVLIERNLRLVAHVIKKFENSGEDPEDLI
SIGTIGLIKAINTYNYRKGTRLATYAARCIENES

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor directs the transcription of crystal protein genes, a sporulation-regulated event [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=107, Percent_Identity=37.3831775700935, Blast_Score=64, Evalue=3e-12,
Organism=Escherichia coli, GI1789098, Length=90, Percent_Identity=35.5555555555556, Blast_Score=61, Evalue=2e-11,
Organism=Escherichia coli, GI1789448, Length=73, Percent_Identity=42.4657534246575, Blast_Score=60, Evalue=3e-11,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014209
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 12851; Mature: 12851

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVIGVISLIVKEISLLVSYISSNSFPQPLSAEDERKYLKRLEQGDDKAREVLIERNLR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
LVAHVIKKFENSGEDPEDLISIGTIGLIKAINTYNYRKGTRLATYAARCIENES
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MIPVIGVISLIVKEISLLVSYISSNSFPQPLSAEDERKYLKRLEQGDDKAREVLIERNLR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
LVAHVIKKFENSGEDPEDLISIGTIGLIKAINTYNYRKGTRLATYAARCIENES
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]