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The map label for this gene is putP [H]

Identifier: 188586371

GI number: 188586371

Start: 1821896

End: 1823437

Strand: Reverse

Name: putP [H]

Synonym: Nther_1756

Alternate gene names: 188586371

Gene position: 1823437-1821896 (Counterclockwise)

Preceding gene: 188586380

Following gene: 188586370

Centisome position: 57.6

GC content: 38.98

Gene sequence:

>1542_bases
ATGGTCCCCATACCTATAATGTCGACTTTTATAGCATATTTAATCTTTATGGTAATAGTCGGTATAATAACCTATAAAAT
GACTTATACCTTGGATGATTATGTGTTGGCTGGGCGCGGTTTAAATAAATGGGTTGCAGCTATGTCAGCTCAAGCTAGCG
ATATGAGTGGTTGGCTACTCTTAGGATTACCAGGTGCCGCTTATGCCTCTGGCATGGGCCAGTGGAGTATCTGGATGTGT
ATAGGTCTTGCTACAGGAACGATGCTTAATTGGCAGTTTATTGCTAAAAAACTCAGGGCTTATACAGAATTAGCTGGTGA
CAGCATTACATTATCCGAATTCTTCTCAAATAGATTTCGAGATAAGAGTGAAGTACTAAGAATTGTATCTGCTCTATTTA
TTTTAGTATTTTTCTTGTTCTATACTGCGTCTGGCTTGGTTGCCGGGGGGAAATTATTTGAGTCGTTTCTCGGTGTTGAT
TATTATCTTGCTTTAACTATTGGAACTATTGTTATCTTGATTTACACCTTTGTAGGGGGATTTATTGCAGTTACCTGGTT
GGACTTTGTTAATGGTATATTAATGTTTTTTGCTCTGATCATTGCCCCTATAGGAGTTGTCAGTCATCTTGGAGGATTGA
GTGATGTTTTTGCAGAAATTGGTTCCATAAACCCTGATCTATTAGATATAACTCGAGGTGTAAATTATAATTACACTGAC
GATATTTTTTGGGAATCTACAGGCCCTATTGGAGCCATTGGTATTATATCGGCTTTGGCCTGGGGATTGGGATACTTTGG
TCAACCTCATATCTTAGCGAGATTTATGGCCATTAAATCTTTGAGAGCTATTAAAACTTCTCGGTTAATTGCAGTGATAT
GGGTTGTTTTAACATTGATGGGAGCTTCCATGGTGGGCTTTATGGGAATTTCCATTTTTGGAGAAGAAGCACCTCTATAT
GATTCTGAGTGGGTCTTTTTAGAATTGGTTCCATTAATTTTTAACCCGTGGATTGCGGGGATTCTATTGGCAGCAGTGTT
AGCAGCTATCATGAGTACAGTGGACTCACAATTACTTGTTTCTTCTAGTGCCTTAACCCAAGACTTTTACAGGCGATTCT
TGAGAAAAGACGCCACTGAAAAAGAGTTGGTTTGGGGTGGAAGAATTTCTGTCTTAATTATTACTTTAATAGCATATTAT
TTAGCATGGGAAGAAGGCCATGTATTAGAACTAGTTGAATATGCTTGGGCTGGTTTTGGAGCGACCTTTGGCCCTGCTGT
CATAGCTTCTTTGTTTTGGAAAAGAACTACTCGTAACGGAGCTCTGGCAGGAATAGTTGTTGGTGGATTGACGGTGATTT
TATGGGAAATACTGGGTACTCCCTTTGGGCTATATGAGATCGTACCTGGTTTTATTTTGTCACTGGTAGCAATCATTATT
TTTAGTTTGATTGATGATGAGCCTAGTCAAGAAATTTTGGAAGAATTTGAAAGATCCCAAGAGCTTTCACAACCTGGAGC
TGAACTGCCAGAAAAGGAATAA

Upstream 100 bases:

>100_bases
ATACTCTTACGACTGCTACGTGGTTTTGGTTACTTGTACCTATGCCTTTACTGATTGTATGGGCCATTATTAGCTATTTT
GTTGAGAGGAGGAATGAATA

Downstream 100 bases:

>100_bases
TTTCAACTATAATCATGATTACTGGTTTTAGGCGGGTGAAACCGTCTAAAACCAGTAATTATGGATGTTAATATTGCAAT
TGCAATATATAAATAACACA

Product: sodium/proline symporter

Products: NA

Alternate protein names: Proline permease; Propionate transporter [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MVPIPIMSTFIAYLIFMVIVGIITYKMTYTLDDYVLAGRGLNKWVAAMSAQASDMSGWLLLGLPGAAYASGMGQWSIWMC
IGLATGTMLNWQFIAKKLRAYTELAGDSITLSEFFSNRFRDKSEVLRIVSALFILVFFLFYTASGLVAGGKLFESFLGVD
YYLALTIGTIVILIYTFVGGFIAVTWLDFVNGILMFFALIIAPIGVVSHLGGLSDVFAEIGSINPDLLDITRGVNYNYTD
DIFWESTGPIGAIGIISALAWGLGYFGQPHILARFMAIKSLRAIKTSRLIAVIWVVLTLMGASMVGFMGISIFGEEAPLY
DSEWVFLELVPLIFNPWIAGILLAAVLAAIMSTVDSQLLVSSSALTQDFYRRFLRKDATEKELVWGGRISVLIITLIAYY
LAWEEGHVLELVEYAWAGFGATFGPAVIASLFWKRTTRNGALAGIVVGGLTVILWEILGTPFGLYEIVPGFILSLVAIII
FSLIDDEPSQEILEEFERSQELSQPGAELPEKE

Sequences:

>Translated_513_residues
MVPIPIMSTFIAYLIFMVIVGIITYKMTYTLDDYVLAGRGLNKWVAAMSAQASDMSGWLLLGLPGAAYASGMGQWSIWMC
IGLATGTMLNWQFIAKKLRAYTELAGDSITLSEFFSNRFRDKSEVLRIVSALFILVFFLFYTASGLVAGGKLFESFLGVD
YYLALTIGTIVILIYTFVGGFIAVTWLDFVNGILMFFALIIAPIGVVSHLGGLSDVFAEIGSINPDLLDITRGVNYNYTD
DIFWESTGPIGAIGIISALAWGLGYFGQPHILARFMAIKSLRAIKTSRLIAVIWVVLTLMGASMVGFMGISIFGEEAPLY
DSEWVFLELVPLIFNPWIAGILLAAVLAAIMSTVDSQLLVSSSALTQDFYRRFLRKDATEKELVWGGRISVLIITLIAYY
LAWEEGHVLELVEYAWAGFGATFGPAVIASLFWKRTTRNGALAGIVVGGLTVILWEILGTPFGLYEIVPGFILSLVAIII
FSLIDDEPSQEILEEFERSQELSQPGAELPEKE
>Mature_513_residues
MVPIPIMSTFIAYLIFMVIVGIITYKMTYTLDDYVLAGRGLNKWVAAMSAQASDMSGWLLLGLPGAAYASGMGQWSIWMC
IGLATGTMLNWQFIAKKLRAYTELAGDSITLSEFFSNRFRDKSEVLRIVSALFILVFFLFYTASGLVAGGKLFESFLGVD
YYLALTIGTIVILIYTFVGGFIAVTWLDFVNGILMFFALIIAPIGVVSHLGGLSDVFAEIGSINPDLLDITRGVNYNYTD
DIFWESTGPIGAIGIISALAWGLGYFGQPHILARFMAIKSLRAIKTSRLIAVIWVVLTLMGASMVGFMGISIFGEEAPLY
DSEWVFLELVPLIFNPWIAGILLAAVLAAIMSTVDSQLLVSSSALTQDFYRRFLRKDATEKELVWGGRISVLIITLIAYY
LAWEEGHVLELVEYAWAGFGATFGPAVIASLFWKRTTRNGALAGIVVGGLTVILWEILGTPFGLYEIVPGFILSLVAIII
FSLIDDEPSQEILEEFERSQELSQPGAELPEKE

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=505, Percent_Identity=41.5841584158416, Blast_Score=394, Evalue=1e-110,
Organism=Homo sapiens, GI14140236, Length=459, Percent_Identity=25.0544662309368, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI110835708, Length=535, Percent_Identity=25.0467289719626, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI17941285, Length=473, Percent_Identity=23.8900634249471, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4507031, Length=454, Percent_Identity=23.568281938326, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI206597483, Length=461, Percent_Identity=24.7288503253796, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI109659836, Length=446, Percent_Identity=23.0941704035874, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI206597487, Length=486, Percent_Identity=23.0452674897119, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI11141885, Length=426, Percent_Identity=24.1784037558685, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI4507033, Length=419, Percent_Identity=23.3890214797136, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI109659839, Length=462, Percent_Identity=22.7272727272727, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1787251, Length=519, Percent_Identity=47.7842003853565, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI87082237, Length=481, Percent_Identity=29.7297297297297, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1790503, Length=468, Percent_Identity=26.2820512820513, Blast_Score=126, Evalue=3e-30,
Organism=Drosophila melanogaster, GI221459584, Length=391, Percent_Identity=22.5063938618926, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI221459582, Length=394, Percent_Identity=25.1269035532995, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 56372; Mature: 56372

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPIPIMSTFIAYLIFMVIVGIITYKMTYTLDDYVLAGRGLNKWVAAMSAQASDMSGWLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHCCCHHHHHHHHHCCCCCCCCEEE
LGLPGAAYASGMGQWSIWMCIGLATGTMLNWQFIAKKLRAYTELAGDSITLSEFFSNRFR
EECCCHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCC
DKSEVLRIVSALFILVFFLFYTASGLVAGGKLFESFLGVDYYLALTIGTIVILIYTFVGG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
FIAVTWLDFVNGILMFFALIIAPIGVVSHLGGLSDVFAEIGSINPDLLDITRGVNYNYTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHCCCCCCCCC
DIFWESTGPIGAIGIISALAWGLGYFGQPHILARFMAIKSLRAIKTSRLIAVIWVVLTLM
CCEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GASMVGFMGISIFGEEAPLYDSEWVFLELVPLIFNPWIAGILLAAVLAAIMSTVDSQLLV
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSSALTQDFYRRFLRKDATEKELVWGGRISVLIITLIAYYLAWEEGHVLELVEYAWAGFG
HHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
ATFGPAVIASLFWKRTTRNGALAGIVVGGLTVILWEILGTPFGLYEIVPGFILSLVAIII
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
FSLIDDEPSQEILEEFERSQELSQPGAELPEKE
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure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HHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3302614; 8905232; 9278503; 1400239; 3325781; 3053687; 3053649; 1567896; 8119910 [H]