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The map label for this gene is dacF [H]

Identifier: 188586281

GI number: 188586281

Start: 1739910

End: 1741097

Strand: Reverse

Name: dacF [H]

Synonym: Nther_1664

Alternate gene names: 188586281

Gene position: 1741097-1739910 (Counterclockwise)

Preceding gene: 188586282

Following gene: 188586280

Centisome position: 55.0

GC content: 36.03

Gene sequence:

>1188_bases
TTGGTCAGGAAAATTAGTTTAGTTTTAGTTTTATTTTTAGCAATAAGTTTAGTTTTTCAAGGATCAACTGCAAAAACAAG
TCCGGAAGACGAATTAGAGGCACCTGTCTATCTTTTAGGTGAACCGAAATCGGGTGAGATTCTCTTAGAACAAGATAAAC
AGGAACAAGTATATCCAGCTAGTATAACTAAAATAATGACATTGCTGCTGACTATGGAAGCTTTAGAAAATGAGCAAATA
GCTTTGGAAGAAGAAGTTATTGTTCCTAGAGAAGCCGAAGACATTCCCGGTACCACAATATTTTTGCAACAAGGTGAAAA
AATAACGGTAGATGATCTAATAACAGCCATAGCAGTTGGTTCGGCAAACGATGCTTCAATTGCAATAGCTAAGCATATCT
CTGGTACTGTAGATAATTTCGTAGATGAAATGAATGATAAAGCTAGAGAACTAGGAATGAAAAACACTAATTTTGAAAAT
CCCCATGGCCTCCATCATGACGAACATGTAACCACAGCCGAGGATATATTTATTATGGCGTCTGAACTGGGAAAATATGA
AGAAATTCACGATTGGCTTGAAATTTGGCTAATTGAAGATTTCTTACAGGGCAAAAAAGTGGTTGAAGATGATGAAGAGG
GAATTTTTTTGAGTAATACCAACAGATTAGTTAATCAATACCCTGGCTGTGATGGATTTAAGACCGGATTTACTGAAGAA
TCCAAACACTCCATTACTGCGACAGCAAAACAAAACGGTGAACGTTATATAGCAATTATCATGAGTCATGAGGATTCAGA
TGGAAGATTTGAAGAAGCTATGAACCTGCTGGATTATGGTTTTGCTAATTATCAAACAAATACAATCCTTGAAAAAGGGG
ATTTGGTTGCCAGAGTAAAAATTAACAAAGGTGATCAAGAGTATCTTCCATTATTAGTTAAGGAAGAACTCGTTGCGGTG
ACTGAGGAATCAGATGAAATTGAAATAGAAGAAGAACATCAAATTCCTGATGATCTTTCCCCTCCCATAGTTGAAGGTGA
TAAAGTTGGTGAATATCAAATTTCATATGGGAATAAGGAATCCCAAGTGGCTTTAATTGCAGGTGAAGATGTTAAGGAAG
CAGGGATAACAGACCACTTTTCTAGATTAATTAAAAATTGGATGAGTTTTTGGACTGGCGAAAAATGA

Upstream 100 bases:

>100_bases
TATTTTTTCCACACTATAGATAACAAATATTAGAGAATTAGTAACAAGTTAAATTCACAAACCGATCAACCATTTACGAC
ATTTCAAGGGGGTAACTAAG

Downstream 100 bases:

>100_bases
GGAAAAATTGCACTTTGTGCAATTTTTCCTCTTATGGAAGGAGTTTTCTTTACAGGAGTAGAACAAAACTTTACCTGAAA
ACGTTGTAATTTAATTACTA

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MVRKISLVLVLFLAISLVFQGSTAKTSPEDELEAPVYLLGEPKSGEILLEQDKQEQVYPASITKIMTLLLTMEALENEQI
ALEEEVIVPREAEDIPGTTIFLQQGEKITVDDLITAIAVGSANDASIAIAKHISGTVDNFVDEMNDKARELGMKNTNFEN
PHGLHHDEHVTTAEDIFIMASELGKYEEIHDWLEIWLIEDFLQGKKVVEDDEEGIFLSNTNRLVNQYPGCDGFKTGFTEE
SKHSITATAKQNGERYIAIIMSHEDSDGRFEEAMNLLDYGFANYQTNTILEKGDLVARVKINKGDQEYLPLLVKEELVAV
TEESDEIEIEEEHQIPDDLSPPIVEGDKVGEYQISYGNKESQVALIAGEDVKEAGITDHFSRLIKNWMSFWTGEK

Sequences:

>Translated_395_residues
MVRKISLVLVLFLAISLVFQGSTAKTSPEDELEAPVYLLGEPKSGEILLEQDKQEQVYPASITKIMTLLLTMEALENEQI
ALEEEVIVPREAEDIPGTTIFLQQGEKITVDDLITAIAVGSANDASIAIAKHISGTVDNFVDEMNDKARELGMKNTNFEN
PHGLHHDEHVTTAEDIFIMASELGKYEEIHDWLEIWLIEDFLQGKKVVEDDEEGIFLSNTNRLVNQYPGCDGFKTGFTEE
SKHSITATAKQNGERYIAIIMSHEDSDGRFEEAMNLLDYGFANYQTNTILEKGDLVARVKINKGDQEYLPLLVKEELVAV
TEESDEIEIEEEHQIPDDLSPPIVEGDKVGEYQISYGNKESQVALIAGEDVKEAGITDHFSRLIKNWMSFWTGEK
>Mature_395_residues
MVRKISLVLVLFLAISLVFQGSTAKTSPEDELEAPVYLLGEPKSGEILLEQDKQEQVYPASITKIMTLLLTMEALENEQI
ALEEEVIVPREAEDIPGTTIFLQQGEKITVDDLITAIAVGSANDASIAIAKHISGTVDNFVDEMNDKARELGMKNTNFEN
PHGLHHDEHVTTAEDIFIMASELGKYEEIHDWLEIWLIEDFLQGKKVVEDDEEGIFLSNTNRLVNQYPGCDGFKTGFTEE
SKHSITATAKQNGERYIAIIMSHEDSDGRFEEAMNLLDYGFANYQTNTILEKGDLVARVKINKGDQEYLPLLVKEELVAV
TEESDEIEIEEEHQIPDDLSPPIVEGDKVGEYQISYGNKESQVALIAGEDVKEAGITDHFSRLIKNWMSFWTGEK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=397, Percent_Identity=29.7229219143577, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1787062, Length=410, Percent_Identity=27.8048780487805, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1786851, Length=375, Percent_Identity=25.8666666666667, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI87082054, Length=219, Percent_Identity=26.027397260274, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44283; Mature: 44283

Theoretical pI: Translated: 4.09; Mature: 4.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRKISLVLVLFLAISLVFQGSTAKTSPEDELEAPVYLLGEPKSGEILLEQDKQEQVYPA
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEECCCCCCEEEECCCCCCCCHH
SITKIMTLLLTMEALENEQIALEEEVIVPREAEDIPGTTIFLQQGEKITVDDLITAIAVG
HHHHHHHHHHHHHHHCCCCEEECCCEECCCCCCCCCCCEEEEECCCEEEHHHHHHHHHCC
SANDASIAIAKHISGTVDNFVDEMNDKARELGMKNTNFENPHGLHHDEHVTTAEDIFIMA
CCCCCEEEEHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SELGKYEEIHDWLEIWLIEDFLQGKKVVEDDEEGIFLSNTNRLVNQYPGCDGFKTGFTEE
HHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHCCCCCCCCCCCCCCC
SKHSITATAKQNGERYIAIIMSHEDSDGRFEEAMNLLDYGFANYQTNTILEKGDLVARVK
CCCCEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEE
INKGDQEYLPLLVKEELVAVTEESDEIEIEEEHQIPDDLSPPIVEGDKVGEYQISYGNKE
ECCCCHHHHHHHHHHHHHHEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCC
SQVALIAGEDVKEAGITDHFSRLIKNWMSFWTGEK
CEEEEEECCCHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVRKISLVLVLFLAISLVFQGSTAKTSPEDELEAPVYLLGEPKSGEILLEQDKQEQVYPA
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEECCCCCCEEEECCCCCCCCHH
SITKIMTLLLTMEALENEQIALEEEVIVPREAEDIPGTTIFLQQGEKITVDDLITAIAVG
HHHHHHHHHHHHHHHCCCCEEECCCEECCCCCCCCCCCEEEEECCCEEEHHHHHHHHHCC
SANDASIAIAKHISGTVDNFVDEMNDKARELGMKNTNFENPHGLHHDEHVTTAEDIFIMA
CCCCCEEEEHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SELGKYEEIHDWLEIWLIEDFLQGKKVVEDDEEGIFLSNTNRLVNQYPGCDGFKTGFTEE
HHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHCCCCCCCCCCCCCCC
SKHSITATAKQNGERYIAIIMSHEDSDGRFEEAMNLLDYGFANYQTNTILEKGDLVARVK
CCCCEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEE
INKGDQEYLPLLVKEELVAVTEESDEIEIEEEHQIPDDLSPPIVEGDKVGEYQISYGNKE
ECCCCHHHHHHHHHHHHHHEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCC
SQVALIAGEDVKEAGITDHFSRLIKNWMSFWTGEK
CEEEEEECCCHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]