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The map label for this gene is rsuA [C]

Identifier: 188586260

GI number: 188586260

Start: 1722728

End: 1723444

Strand: Reverse

Name: rsuA [C]

Synonym: Nther_1643

Alternate gene names: 188586260

Gene position: 1723444-1722728 (Counterclockwise)

Preceding gene: 188586261

Following gene: 188586259

Centisome position: 54.44

GC content: 32.08

Gene sequence:

>717_bases
ATGCGCTTACAAAAATTCATGTCAAGGGCTGGAATAGCATCTAGACGCAAGTCAGAAGAACTCATCCAGCAGGGAAGAGT
TACAGTTAATGGACAAGTTATTACACGTTTAGGAACTAAAATAAACCCTGATACGGATACGGTTAAAGTTGATGGCGAAC
CAATAACTCAAGAGGAATCTAATGTTTATATCATGTTGAACAAGCCCAAAGATTATATAACCAGTAAAGATGACCCTCAA
GGGAGAAAAACTGTCATGGATTTAATTCAGGTGAAAGAAAGAGTTTACCCAGTAGGTAGGTTAGATTACGATTCAAGAGG
ATTAGTTTTACTTACCAATGATGGAGAATTAGCAAATAGATTAATGCATCCTAAATACAATATCCCCAAAACTTATATGG
TTAAAGTAGACAAACTTCCTTTACAGGATAAACTTGATAATCTAGTTAATGGGATTTATTTAGAAGAAGGACTATGTAAA
GCAGATCATGTGGAAATACTAGATACTAAAAATAATATATTAAAAATTATATTATCAGAAGGAAGAAAGAGACAGATTAG
ACGCATGTTAGCTAGTTTAGGAATAGAAGTTAAGGATTTAAAAAGGGACAGCTTAGGAACTTTAACTTTAAAAGACTTAT
CTGAGGGTAATTATAGAAAATTAACAAAAGATGAAATAGATAACATAAAAAAATTAGCTGGTTTGACTGATAATTAA

Upstream 100 bases:

>100_bases
TATGATAGGATTAATTTTTAGCACTTTATTTGCTTGTATAATTAATTAAATTATATTGGCTAATAATAGAAATAGAGCAA
CTAAAAAGGGAGGTAAACTA

Downstream 100 bases:

>100_bases
TATAAAGATATAACTATCCTATACGCTCAAGCTGCTAGTATTAATGGTCTTTCTATTGGTAATAATGAAAATAATTATAC
TAGTATGGAAAGGGGGATGT

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRLQKFMSRAGIASRRKSEELIQQGRVTVNGQVITRLGTKINPDTDTVKVDGEPITQEESNVYIMLNKPKDYITSKDDPQ
GRKTVMDLIQVKERVYPVGRLDYDSRGLVLLTNDGELANRLMHPKYNIPKTYMVKVDKLPLQDKLDNLVNGIYLEEGLCK
ADHVEILDTKNNILKIILSEGRKRQIRRMLASLGIEVKDLKRDSLGTLTLKDLSEGNYRKLTKDEIDNIKKLAGLTDN

Sequences:

>Translated_238_residues
MRLQKFMSRAGIASRRKSEELIQQGRVTVNGQVITRLGTKINPDTDTVKVDGEPITQEESNVYIMLNKPKDYITSKDDPQ
GRKTVMDLIQVKERVYPVGRLDYDSRGLVLLTNDGELANRLMHPKYNIPKTYMVKVDKLPLQDKLDNLVNGIYLEEGLCK
ADHVEILDTKNNILKIILSEGRKRQIRRMLASLGIEVKDLKRDSLGTLTLKDLSEGNYRKLTKDEIDNIKKLAGLTDN
>Mature_238_residues
MRLQKFMSRAGIASRRKSEELIQQGRVTVNGQVITRLGTKINPDTDTVKVDGEPITQEESNVYIMLNKPKDYITSKDDPQ
GRKTVMDLIQVKERVYPVGRLDYDSRGLVLLTNDGELANRLMHPKYNIPKTYMVKVDKLPLQDKLDNLVNGIYLEEGLCK
ADHVEILDTKNNILKIILSEGRKRQIRRMLASLGIEVKDLKRDSLGTLTLKDLSEGNYRKLTKDEIDNIKKLAGLTDN

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=35.8974358974359, Blast_Score=150, Evalue=7e-38,
Organism=Escherichia coli, GI1790453, Length=234, Percent_Identity=35.4700854700855, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1787524, Length=244, Percent_Identity=35.2459016393443, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=38.0681818181818, Blast_Score=113, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27123; Mature: 27123

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLQKFMSRAGIASRRKSEELIQQGRVTVNGQVITRLGTKINPDTDTVKVDGEPITQEES
CCHHHHHHHHCHHHHHHHHHHHHCCCEEECCEEEEECCCCCCCCCCEEEECCCCCCCCCC
NVYIMLNKPKDYITSKDDPQGRKTVMDLIQVKERVYPVGRLDYDSRGLVLLTNDGELANR
CEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHH
LMHPKYNIPKTYMVKVDKLPLQDKLDNLVNGIYLEEGLCKADHVEILDTKNNILKIILSE
HCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCHHHCCCCCCCCEEEEECCCHHHHHHHHC
GRKRQIRRMLASLGIEVKDLKRDSLGTLTLKDLSEGNYRKLTKDEIDNIKKLAGLTDN
CHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRLQKFMSRAGIASRRKSEELIQQGRVTVNGQVITRLGTKINPDTDTVKVDGEPITQEES
CCHHHHHHHHCHHHHHHHHHHHHCCCEEECCEEEEECCCCCCCCCCEEEECCCCCCCCCC
NVYIMLNKPKDYITSKDDPQGRKTVMDLIQVKERVYPVGRLDYDSRGLVLLTNDGELANR
CEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHH
LMHPKYNIPKTYMVKVDKLPLQDKLDNLVNGIYLEEGLCKADHVEILDTKNNILKIILSE
HCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCHHHCCCCCCCCEEEEECCCHHHHHHHHC
GRKRQIRRMLASLGIEVKDLKRDSLGTLTLKDLSEGNYRKLTKDEIDNIKKLAGLTDN
CHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]