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The map label for this gene is hemC

Identifier: 188585750

GI number: 188585750

Start: 1191258

End: 1192193

Strand: Direct

Name: hemC

Synonym: Nther_1124

Alternate gene names: 188585750

Gene position: 1191258-1192193 (Clockwise)

Preceding gene: 188585749

Following gene: 188585751

Centisome position: 37.63

GC content: 37.29

Gene sequence:

>936_bases
ATGAAACTAAGAATAGGGACTAGAAGGAGCCAACTTGCCCTTGACCAAACTAATTGGGTAGTAGAACAATTAAAAACGCA
TTATCCAGACATAGAAATTGAAATTAAAAAAATTGAAACAAAGGGTGATCAGTTACTAAATGTTTCTCTATCCAAAGTTG
GTGGCAAGGGTTTATTTTTAAAAGAGATCCAAAATGCCTTACTTCAAGGAGAAATCGATTTGGCTGTTCATAGTATGAAA
GATATACCTACGGAAACTACTGAAGATCTTGAAATTTGTGCCATCACTAAGAGAGTCGATCCTCTTGATGCCCTGATTTC
CAATAATGATATTACAATAGACGAACTGCCTGAAAATGCTAAGATAGGAACTAGCAGTTTAAGGCGTGGTTCACAGTTAA
AAGCTTACAGAAATGACTTACAAATAATTCCTATTAGAGGAAATATTAATACCAGAATGAATAAGCTCCAAGAACTTCCA
GAGTTAGATGCCGTGGTCCTAGCAAAAGCAGGGCTTGTTAGATCAGGGATGACAGACAGTATTAGCCAGAATATATCTCC
AGAAATAATAGTTCCCTGTCCCGGTCAAGGTGCTTTAGGATTAGAGATTCGCCATGATAACGAGAATCTCAAAGAAAAAC
TAGCAGTATTAGATGATTCGGAAAGCAGAAAGGCAATTGGGGCAGAAAGAGCTTTTCTTAATAGATTAGGTGGTAGCTGC
CATGTGCCTGTAGGAGCTTATGCTGAAATTATTTCAAATGAACTACATTTGACAGGAGTAGTTGCAAGTGAGGATGGGCA
AGATGTAATAAAAAGAAGTGTTCAAACTCATCTTAGTGATAATAACAAAATACCATCTACATTGGGAAATGACCTGGCTG
AAGAGCTGATAGAGTTAGGAGCAAATAAAATATTGTCAGAACTTAAGGAGGGGTAG

Upstream 100 bases:

>100_bases
GATGCAATATTTGACCAGGTCAAAGATACTAATAGAAAAGACCTGGAATATTTTTGTGATAAATTACTAAAACAGTTAAT
AGAGAATGGGAGGCAACAAG

Downstream 100 bases:

>100_bases
ATGTGTCAGGGAGAGTATATTTAATAGGTGCGGGCCCAGGAGATGAAGGCCTAATTACTGTCAAAGGCCTAAACTGTGTC
AAAGAAGCTAATGTAATTGT

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKLRIGTRRSQLALDQTNWVVEQLKTHYPDIEIEIKKIETKGDQLLNVSLSKVGGKGLFLKEIQNALLQGEIDLAVHSMK
DIPTETTEDLEICAITKRVDPLDALISNNDITIDELPENAKIGTSSLRRGSQLKAYRNDLQIIPIRGNINTRMNKLQELP
ELDAVVLAKAGLVRSGMTDSISQNISPEIIVPCPGQGALGLEIRHDNENLKEKLAVLDDSESRKAIGAERAFLNRLGGSC
HVPVGAYAEIISNELHLTGVVASEDGQDVIKRSVQTHLSDNNKIPSTLGNDLAEELIELGANKILSELKEG

Sequences:

>Translated_311_residues
MKLRIGTRRSQLALDQTNWVVEQLKTHYPDIEIEIKKIETKGDQLLNVSLSKVGGKGLFLKEIQNALLQGEIDLAVHSMK
DIPTETTEDLEICAITKRVDPLDALISNNDITIDELPENAKIGTSSLRRGSQLKAYRNDLQIIPIRGNINTRMNKLQELP
ELDAVVLAKAGLVRSGMTDSISQNISPEIIVPCPGQGALGLEIRHDNENLKEKLAVLDDSESRKAIGAERAFLNRLGGSC
HVPVGAYAEIISNELHLTGVVASEDGQDVIKRSVQTHLSDNNKIPSTLGNDLAEELIELGANKILSELKEG
>Mature_311_residues
MKLRIGTRRSQLALDQTNWVVEQLKTHYPDIEIEIKKIETKGDQLLNVSLSKVGGKGLFLKEIQNALLQGEIDLAVHSMK
DIPTETTEDLEICAITKRVDPLDALISNNDITIDELPENAKIGTSSLRRGSQLKAYRNDLQIIPIRGNINTRMNKLQELP
ELDAVVLAKAGLVRSGMTDSISQNISPEIIVPCPGQGALGLEIRHDNENLKEKLAVLDDSESRKAIGAERAFLNRLGGSC
HVPVGAYAEIISNELHLTGVVASEDGQDVIKRSVQTHLSDNNKIPSTLGNDLAEELIELGANKILSELKEG

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=276, Percent_Identity=44.2028985507246, Blast_Score=227, Evalue=9e-60,
Organism=Homo sapiens, GI66933009, Length=276, Percent_Identity=44.2028985507246, Blast_Score=227, Evalue=9e-60,
Organism=Escherichia coli, GI48994974, Length=309, Percent_Identity=44.336569579288, Blast_Score=249, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6319996, Length=317, Percent_Identity=35.0157728706625, Blast_Score=183, Evalue=2e-47,
Organism=Drosophila melanogaster, GI20130425, Length=291, Percent_Identity=38.4879725085911, Blast_Score=187, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_NATTJ (B2A1G7)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917295.1
- ProteinModelPortal:   B2A1G7
- SMR:   B2A1G7
- GeneID:   6315353
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1124
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34055; Mature: 34055

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRIGTRRSQLALDQTNWVVEQLKTHYPDIEIEIKKIETKGDQLLNVSLSKVGGKGLFL
CEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEHHHHCCCCCEEH
KEIQNALLQGEIDLAVHSMKDIPTETTEDLEICAITKRVDPLDALISNNDITIDELPENA
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEEHHCCCHHHHHHCCCCEEHCCCCCCC
KIGTSSLRRGSQLKAYRNDLQIIPIRGNINTRMNKLQELPELDAVVLAKAGLVRSGMTDS
CCCHHHHHCCCHHHHHHCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHH
ISQNISPEIIVPCPGQGALGLEIRHDNENLKEKLAVLDDSESRKAIGAERAFLNRLGGSC
HHHCCCCCEEEECCCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
HVPVGAYAEIISNELHLTGVVASEDGQDVIKRSVQTHLSDNNKIPSTLGNDLAEELIELG
CCCHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
ANKILSELKEG
HHHHHHHHHCC
>Mature Secondary Structure
MKLRIGTRRSQLALDQTNWVVEQLKTHYPDIEIEIKKIETKGDQLLNVSLSKVGGKGLFL
CEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEHHHHCCCCCEEH
KEIQNALLQGEIDLAVHSMKDIPTETTEDLEICAITKRVDPLDALISNNDITIDELPENA
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEEHHCCCHHHHHHCCCCEEHCCCCCCC
KIGTSSLRRGSQLKAYRNDLQIIPIRGNINTRMNKLQELPELDAVVLAKAGLVRSGMTDS
CCCHHHHHCCCHHHHHHCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHH
ISQNISPEIIVPCPGQGALGLEIRHDNENLKEKLAVLDDSESRKAIGAERAFLNRLGGSC
HHHCCCCCEEEECCCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
HVPVGAYAEIISNELHLTGVVASEDGQDVIKRSVQTHLSDNNKIPSTLGNDLAEELIELG
CCCHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
ANKILSELKEG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA