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The map label for this gene is tig

Identifier: 188585727

GI number: 188585727

Start: 1163632

End: 1164972

Strand: Direct

Name: tig

Synonym: Nther_1101

Alternate gene names: 188585727

Gene position: 1163632-1164972 (Clockwise)

Preceding gene: 188585726

Following gene: 188585728

Centisome position: 36.76

GC content: 35.94

Gene sequence:

>1341_bases
ATGAGCAGTACTTGGGAAAAGATAGACAAGAACAAAATCAAGCTGTCTGTTGAAGTTGATGAAAATAGGGTTGAAGACGC
CCTGGAGCAAGCTTACAAAAAAGTAGTCAAACAGGTGGAAATCCCTGGGTTCAGAAAGGGGAAAGTGCCCAGAAAAATCT
TAGAAAATAGGTTTGGACCAGAAGTTCTATACGAAGATGCTATAGAGATACTTGTTCCTGAAGCTTATCAGGAAGCGTTA
GAGGAACATGAAATTGAGCCCGTGGATCAACCTGAAATTGATATAGACCAAATGGAAAAGGGGCAACCTTTGAAGTTTAA
TGCCACTGTAGAAGTAAAACCAGAAGTTGAACTTGGTACTTATAAAGGACTAGAAGTAGAAAAGGAAAAAGTCGAAGTTA
CTGAAGAAGATGTGGAAAATGAATTGAAACAAATGCAAGAACAACATGCTGAATATGAAGATGTTGAAGACGGTGAAGCG
GAAAATGGAGATAGACTGGTTATCGATTTTGAAGGCTATGTGGACGGAGAACCCATTGAGGGTGGTCAGGCCGAAAATCA
TAATATAGAGCTAGGATCAAATCAGTTTATTCCTGGATTTGAAGAACAATTAGTAGGTAGTAAACCTGGAGAAGAGAAAG
AAGTAAAAGTAACATTTCCTGAAGACTATCAAAACGAAGAATTAAAAGGGAAAGAAGCTACTTTTAATGTAAAAGTTAAA
GAAATTAAAAAGAAAAACCTATTACCTATAGATGACGAATTTGCAAAAGATGTTAGTGATTTTGATACATTAGAAGAATT
TAAAAATGATATAAGAAATAGGCTAGAAGAAGAAGCTGAAAGGGCTGCCGAACAACAAGTTGAAGAACAGGTTGTTACTA
AAGCCCTGGAAAATGCCGAAGTAGAAATTCCACAACCTATGATTGACCAAGAAGTAGATAATATGCTTAAAGAGTTTGAA
CAAAACTTATCATATCAGGGATTGAATTTAGACACTTATTACAAATTGGCAAATACAGACGAAGATGCTATGAAGGAACA
ATTTAAGGGGTCAGCTGAAACTCGAGTAAAAAGAAATCTTGTTTTAGAAGCCATCAAAGATGAGGAAGGAATTACAGCCA
CTGAAGAAGAAATAGATGAAGAAATCAACAAAATAGCTGAACAGGCACAACAAGAAGCTGATAAGATTAAGGAATTCCTA
GAAATGCAAGGTAGAATGTCTCAATTGAAAAATGAAATAGCTATAAGAAAGTCAGTTGATTTGCTGAAAGACGAAGCAAA
GGTCACTGTAGTTGAGAAAAGTAATGATTCTGAGGAAGAAACTCAAGGGAACACAGAATAA

Upstream 100 bases:

>100_bases
ATTTATAAATTCTGACACCTTTTACCTGGCACTATTATTTATGTTATAATGTGGAGGTATTAATTGGACATGATTACAAT
TAAAGAAGGAGGAAGTATAA

Downstream 100 bases:

>100_bases
TAAAACTAAAAATCTTAACTATAAGGTTTTCAACTATCAGCCAAATTGATAAAAGTTATTAGTTTAAAAAGGGAGACCTA
AAGACAGAAATATGTTATTA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSSTWEKIDKNKIKLSVEVDENRVEDALEQAYKKVVKQVEIPGFRKGKVPRKILENRFGPEVLYEDAIEILVPEAYQEAL
EEHEIEPVDQPEIDIDQMEKGQPLKFNATVEVKPEVELGTYKGLEVEKEKVEVTEEDVENELKQMQEQHAEYEDVEDGEA
ENGDRLVIDFEGYVDGEPIEGGQAENHNIELGSNQFIPGFEEQLVGSKPGEEKEVKVTFPEDYQNEELKGKEATFNVKVK
EIKKKNLLPIDDEFAKDVSDFDTLEEFKNDIRNRLEEEAERAAEQQVEEQVVTKALENAEVEIPQPMIDQEVDNMLKEFE
QNLSYQGLNLDTYYKLANTDEDAMKEQFKGSAETRVKRNLVLEAIKDEEGITATEEEIDEEINKIAEQAQQEADKIKEFL
EMQGRMSQLKNEIAIRKSVDLLKDEAKVTVVEKSNDSEEETQGNTE

Sequences:

>Translated_446_residues
MSSTWEKIDKNKIKLSVEVDENRVEDALEQAYKKVVKQVEIPGFRKGKVPRKILENRFGPEVLYEDAIEILVPEAYQEAL
EEHEIEPVDQPEIDIDQMEKGQPLKFNATVEVKPEVELGTYKGLEVEKEKVEVTEEDVENELKQMQEQHAEYEDVEDGEA
ENGDRLVIDFEGYVDGEPIEGGQAENHNIELGSNQFIPGFEEQLVGSKPGEEKEVKVTFPEDYQNEELKGKEATFNVKVK
EIKKKNLLPIDDEFAKDVSDFDTLEEFKNDIRNRLEEEAERAAEQQVEEQVVTKALENAEVEIPQPMIDQEVDNMLKEFE
QNLSYQGLNLDTYYKLANTDEDAMKEQFKGSAETRVKRNLVLEAIKDEEGITATEEEIDEEINKIAEQAQQEADKIKEFL
EMQGRMSQLKNEIAIRKSVDLLKDEAKVTVVEKSNDSEEETQGNTE
>Mature_445_residues
SSTWEKIDKNKIKLSVEVDENRVEDALEQAYKKVVKQVEIPGFRKGKVPRKILENRFGPEVLYEDAIEILVPEAYQEALE
EHEIEPVDQPEIDIDQMEKGQPLKFNATVEVKPEVELGTYKGLEVEKEKVEVTEEDVENELKQMQEQHAEYEDVEDGEAE
NGDRLVIDFEGYVDGEPIEGGQAENHNIELGSNQFIPGFEEQLVGSKPGEEKEVKVTFPEDYQNEELKGKEATFNVKVKE
IKKKNLLPIDDEFAKDVSDFDTLEEFKNDIRNRLEEEAERAAEQQVEEQVVTKALENAEVEIPQPMIDQEVDNMLKEFEQ
NLSYQGLNLDTYYKLANTDEDAMKEQFKGSAETRVKRNLVLEAIKDEEGITATEEEIDEEINKIAEQAQQEADKIKEFLE
MQGRMSQLKNEIAIRKSVDLLKDEAKVTVVEKSNDSEEETQGNTE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=421, Percent_Identity=31.1163895486936, Blast_Score=188, Evalue=7e-49,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_NATTJ (B2A157)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917272.1
- ProteinModelPortal:   B2A157
- GeneID:   6314984
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1101
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 51227; Mature: 51096

Theoretical pI: Translated: 4.04; Mature: 4.04

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTWEKIDKNKIKLSVEVDENRVEDALEQAYKKVVKQVEIPGFRKGKVPRKILENRFGP
CCCHHHHHCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCH
EVLYEDAIEILVPEAYQEALEEHEIEPVDQPEIDIDQMEKGQPLKFNATVEVKPEVELGT
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEECEEEEECCCEECCC
YKGLEVEKEKVEVTEEDVENELKQMQEQHAEYEDVEDGEAENGDRLVIDFEGYVDGEPIE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
GGQAENHNIELGSNQFIPGFEEQLVGSKPGEEKEVKVTFPEDYQNEELKGKEATFNVKVK
CCCCCCCCEEECCCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEHH
EIKKKNLLPIDDEFAKDVSDFDTLEEFKNDIRNRLEEEAERAAEQQVEEQVVTKALENAE
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VEIPQPMIDQEVDNMLKEFEQNLSYQGLNLDTYYKLANTDEDAMKEQFKGSAETRVKRNL
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHEEEECCCCHHHHHHHHCCCHHHHHHHHH
VLEAIKDEEGITATEEEIDEEINKIAEQAQQEADKIKEFLEMQGRMSQLKNEIAIRKSVD
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLKDEAKVTVVEKSNDSEEETQGNTE
HHHCCCEEEEEECCCCCHHHCCCCCC
>Mature Secondary Structure 
SSTWEKIDKNKIKLSVEVDENRVEDALEQAYKKVVKQVEIPGFRKGKVPRKILENRFGP
CCHHHHHCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCH
EVLYEDAIEILVPEAYQEALEEHEIEPVDQPEIDIDQMEKGQPLKFNATVEVKPEVELGT
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEECEEEEECCCEECCC
YKGLEVEKEKVEVTEEDVENELKQMQEQHAEYEDVEDGEAENGDRLVIDFEGYVDGEPIE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
GGQAENHNIELGSNQFIPGFEEQLVGSKPGEEKEVKVTFPEDYQNEELKGKEATFNVKVK
CCCCCCCCEEECCCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEHH
EIKKKNLLPIDDEFAKDVSDFDTLEEFKNDIRNRLEEEAERAAEQQVEEQVVTKALENAE
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VEIPQPMIDQEVDNMLKEFEQNLSYQGLNLDTYYKLANTDEDAMKEQFKGSAETRVKRNL
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHEEEECCCCHHHHHHHHCCCHHHHHHHHH
VLEAIKDEEGITATEEEIDEEINKIAEQAQQEADKIKEFLEMQGRMSQLKNEIAIRKSVD
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLKDEAKVTVVEKSNDSEEETQGNTE
HHHCCCEEEEEECCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA