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The map label for this gene is dacF [H]

Identifier: 188584694

GI number: 188584694

Start: 65183

End: 66433

Strand: Direct

Name: dacF [H]

Synonym: Nther_0052

Alternate gene names: 188584694

Gene position: 65183-66433 (Clockwise)

Preceding gene: 188584692

Following gene: 188584695

Centisome position: 2.06

GC content: 36.13

Gene sequence:

>1251_bases
TTGAAAAGTGATACATCTGGCAATAAAAAGGTGATTATTACACTGATTTTTTTATTAATGATTAATACCTTTACGGTTTC
GGATGTAACCGCAAAACAGTTGTATCCTCAGGAGTTCGAGACTCCTGAAATTGAAGCCGAAACAGCAGTTTTGATGGAAT
TTGAATCGGGTAAGGTTTTATATGACCAAGAAATGCATCGACAGATGCACCCTGCTAGTACTACTAAAATCATGACAGCT
TTACTATTGATTGAAAACGCCTCTCTGAGGGAGCAAGTAGTTGTAAGTGAAAATGCTTTTGGAACTGATGGCTCTAGCAT
GTACCTCAATTTAGATGAAAAAATCACTACTCGAGATTTACTATATGGCTTGATGGTTAGATCAGCAAATGATGCTGCAG
TGGCTATTGCCGAATACGTAGCTGGTTCAGTAGAAAACTTTGCTGATATGATGAATAGTAGAGCAAAAGAGCTAGGGGCA
AAGAACACTAATTTTAAAAACCCTCATGGCTTAACTGAAGAAAATCATAAAACTACTGCTTATGACCTGGGTGTGATTGC
TAGAGAAGCTTTGAAAAACCCAAAATTTAGAAGTGTAGCTTCTACTCGTAGGATAACTCTGGATTGGCCTGAGGAAGAAG
ATGAAGACAGGCTGCTAGTTAATAGAAATGAATTGTTAGCTGATTATCCAGGTGCATTGGGTGTAAAAACTGGATATACA
AGATCGGCAAATCAAACTTTTGTTGCGGCTGCTGAAAGGGATGGCATGACATTAATTGCCGTTGTATTGTCAACATCTAG
AAATAGGTTGTTTGAGGATGCCAAAGAATTACTAGATTTTGGTTTTGAAGCCTTTGAACAAGTTGAAATTTTTGAACAAA
ATGACGTTATAACTTCTACTGAAGTAGAAAACTCAAATCAAGAACTTGAATTAATTGTGGAAGAATCTTTGAGTTTACCA
ATACCTAAAATGGCTACTGAAATTGAACAAAGAGTTGATTTAGAAGAACAACTTTTAAAGGCTCCCATAGAAGAAGGTGA
GCAGGTAGGATCTTTGGACCTATTATTGGAAGAAGAGGAAATTGTCTCAGTCCCATTGTATACAAAAGAAGATGTTGAAA
AAGCTCCTCGCTATGGGTTTTGGATTTTGGCTGTAATGATTACTATACCTGCAATTTTCTTGATCTATAAAAGGTATTAT
GAACTTAAGTCGAGAAAAAAATTTCAAGAGAAGCTAAAAAATAGATACTGA

Upstream 100 bases:

>100_bases
ATAATTATTGTTAGTTTGGTAACTCTATTCCTTTTAACTATTGAAAGTTAACTTTCAATTCCGTTATAATAAACTAGTAA
CAGAGAGGGAGGGGAATTTT

Downstream 100 bases:

>100_bases
TATTGAATGGGATGTAAATAAACAATCAGAAAGGAGAAATGCTAATGGATAGAATGGATTCTCTACATAATGTAGAGAAA
TGGGATCCTAAAACAAACCG

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKSDTSGNKKVIITLIFLLMINTFTVSDVTAKQLYPQEFETPEIEAETAVLMEFESGKVLYDQEMHRQMHPASTTKIMTA
LLLIENASLREQVVVSENAFGTDGSSMYLNLDEKITTRDLLYGLMVRSANDAAVAIAEYVAGSVENFADMMNSRAKELGA
KNTNFKNPHGLTEENHKTTAYDLGVIAREALKNPKFRSVASTRRITLDWPEEEDEDRLLVNRNELLADYPGALGVKTGYT
RSANQTFVAAAERDGMTLIAVVLSTSRNRLFEDAKELLDFGFEAFEQVEIFEQNDVITSTEVENSNQELELIVEESLSLP
IPKMATEIEQRVDLEEQLLKAPIEEGEQVGSLDLLLEEEEIVSVPLYTKEDVEKAPRYGFWILAVMITIPAIFLIYKRYY
ELKSRKKFQEKLKNRY

Sequences:

>Translated_416_residues
MKSDTSGNKKVIITLIFLLMINTFTVSDVTAKQLYPQEFETPEIEAETAVLMEFESGKVLYDQEMHRQMHPASTTKIMTA
LLLIENASLREQVVVSENAFGTDGSSMYLNLDEKITTRDLLYGLMVRSANDAAVAIAEYVAGSVENFADMMNSRAKELGA
KNTNFKNPHGLTEENHKTTAYDLGVIAREALKNPKFRSVASTRRITLDWPEEEDEDRLLVNRNELLADYPGALGVKTGYT
RSANQTFVAAAERDGMTLIAVVLSTSRNRLFEDAKELLDFGFEAFEQVEIFEQNDVITSTEVENSNQELELIVEESLSLP
IPKMATEIEQRVDLEEQLLKAPIEEGEQVGSLDLLLEEEEIVSVPLYTKEDVEKAPRYGFWILAVMITIPAIFLIYKRYY
ELKSRKKFQEKLKNRY
>Mature_416_residues
MKSDTSGNKKVIITLIFLLMINTFTVSDVTAKQLYPQEFETPEIEAETAVLMEFESGKVLYDQEMHRQMHPASTTKIMTA
LLLIENASLREQVVVSENAFGTDGSSMYLNLDEKITTRDLLYGLMVRSANDAAVAIAEYVAGSVENFADMMNSRAKELGA
KNTNFKNPHGLTEENHKTTAYDLGVIAREALKNPKFRSVASTRRITLDWPEEEDEDRLLVNRNELLADYPGALGVKTGYT
RSANQTFVAAAERDGMTLIAVVLSTSRNRLFEDAKELLDFGFEAFEQVEIFEQNDVITSTEVENSNQELELIVEESLSLP
IPKMATEIEQRVDLEEQLLKAPIEEGEQVGSLDLLLEEEEIVSVPLYTKEDVEKAPRYGFWILAVMITIPAIFLIYKRYY
ELKSRKKFQEKLKNRY

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=366, Percent_Identity=32.2404371584699, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI87082022, Length=375, Percent_Identity=31.7333333333333, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1786851, Length=387, Percent_Identity=28.4237726098191, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI87082054, Length=212, Percent_Identity=28.7735849056604, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47175; Mature: 47175

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSDTSGNKKVIITLIFLLMINTFTVSDVTAKQLYPQEFETPEIEAETAVLMEFESGKVL
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCEEEEEEECCCCEE
YDQEMHRQMHPASTTKIMTALLLIENASLREQVVVSENAFGTDGSSMYLNLDEKITTRDL
ECHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHEEEECCCCCCCCCEEEEECCCHHHHHHH
LYGLMVRSANDAAVAIAEYVAGSVENFADMMNSRAKELGAKNTNFKNPHGLTEENHKTTA
HHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
YDLGVIAREALKNPKFRSVASTRRITLDWPEEEDEDRLLVNRNELLADYPGALGVKTGYT
HHHHHHHHHHHCCCCHHHHHCCCEEEEECCCCCCCCCEEECCCHHHHCCCCCCCCCCCCC
RSANQTFVAAAERDGMTLIAVVLSTSRNRLFEDAKELLDFGFEAFEQVEIFEQNDVITST
CCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
EVENSNQELELIVEESLSLPIPKMATEIEQRVDLEEQLLKAPIEEGEQVGSLDLLLEEEE
CCCCCCCHHEEEEEHHCCCCCHHHHHHHHHHCCHHHHHHHCHHHCCCCCCCCCEEEECCC
IVSVPLYTKEDVEKAPRYGFWILAVMITIPAIFLIYKRYYELKSRKKFQEKLKNRY
EEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSDTSGNKKVIITLIFLLMINTFTVSDVTAKQLYPQEFETPEIEAETAVLMEFESGKVL
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCEEEEEEECCCCEE
YDQEMHRQMHPASTTKIMTALLLIENASLREQVVVSENAFGTDGSSMYLNLDEKITTRDL
ECHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHEEEECCCCCCCCCEEEEECCCHHHHHHH
LYGLMVRSANDAAVAIAEYVAGSVENFADMMNSRAKELGAKNTNFKNPHGLTEENHKTTA
HHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
YDLGVIAREALKNPKFRSVASTRRITLDWPEEEDEDRLLVNRNELLADYPGALGVKTGYT
HHHHHHHHHHHCCCCHHHHHCCCEEEEECCCCCCCCCEEECCCHHHHCCCCCCCCCCCCC
RSANQTFVAAAERDGMTLIAVVLSTSRNRLFEDAKELLDFGFEAFEQVEIFEQNDVITST
CCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
EVENSNQELELIVEESLSLPIPKMATEIEQRVDLEEQLLKAPIEEGEQVGSLDLLLEEEE
CCCCCCCHHEEEEEHHCCCCCHHHHHHHHHHCCHHHHHHHCHHHCCCCCCCCCEEEECCC
IVSVPLYTKEDVEKAPRYGFWILAVMITIPAIFLIYKRYYELKSRKKFQEKLKNRY
EEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]