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The map label for this gene is gyrB [H]

Identifier: 188584649

GI number: 188584649

Start: 5587

End: 7509

Strand: Direct

Name: gyrB [H]

Synonym: Nther_0007

Alternate gene names: 188584649

Gene position: 5587-7509 (Clockwise)

Preceding gene: 188584648

Following gene: 188584650

Centisome position: 0.18

GC content: 35.31

Gene sequence:

>1923_bases
ATGGAAGAAAACAAGTTTGTTAACGAGCAAAATTATGATGAAAGCCAAATACAAGTCCTTGAAGGTTTAGAGGCCGTTAG
AAAAAGGCCAGGAATGTATATAGGTTCGACCGATGAGCGAGGTTTGCATCACTTGGTTCATGAAGTTGTAGATAACAGTA
TAGATGAGGCTATGGCAGGTTATTGCGATGATATAAGAATTAAATTAGAGCCAGGTAACAAAGTCCGAGTTACTGACAAT
GGTCGAGGAATACCTGTAAAAGATCACCCCAAGATTAAAAAACCAACTTTAGAAGTAATTATGACTTATCTACACGCAGG
CGGTAAGTTTTCAAATGACAGCTATAAAGTATCTGGTGGACTTCACGGTGTAGGTATATCTGTGGTAAATGGTTTATCAA
AGTGGATAAGAGTTAAAGTTTATCGTGATGGTAAAGTCTATCAACAAGAGTATGAAAAAGGTTATCCCAAAACGGAAATT
AAAGAAATAGGAGAGTCGGACAAGCGCGGAACAGAAATCATTTTTCTACCTGATGAAGAAATTTTCGAGACAGTTGAATT
TGACTATCAAGTGCTTAGAAATAGATTACAGCAATTAGCTTTTTTAAATAGAGGAGTTTCAATTACTATTAGCGATGAAA
GAGAAGATGAATTTAAAGAAGAACATTTTAAATATGAGGGTGGTATTAAGTCCTTTGTAGAATATTTAAACGAAAATAAA
ACAACTCTATTTACTCCACCAGTTTATATTGAAGGCGAACAAGAAGGGTGTAAATTAGAAGTAGCAATTCAATATAATGA
CGGCTATCAAGAAATGATTCATTCCTACGCAAATAATATAAATACTCATGAGGGAGGTACTCATGAATCAGGCTTTAAAA
TTAGCCTAACCAGAACGGTTAATGATTACGCACGCCGACATAATCTGCTCAAAAACGGAGACAAAAACTTAACAGGTGAA
GATATTAGAGAAGGTATCACTGCTATTATAAGTGTTAAAGTGGAAGATCCTCAATTTGAAGGACAAACAAAAACTAAACT
GGGAAATAGTGAAGTGAGAAGGATTGTCGAAGGTATTGTTTCGGAACATCTGGAAAGTTATTTAGAAGAAAATCCTGCAG
TAGGTAAAAAAATAGTTAATAAAGCAATTAATGCTTCACGAGCTAGAGAAGCAGCTAGAAAAGCAAAAGAGTTGACCAGA
AGAAAAAATGCCTTAGAGGTAACTGCTCTTCCAGGTAAATTGGCCGATTGTACTACAAAGGATGCTTCAAAAAGTGAATT
GTATTTAGTCGAGGGTGATTCAGCTGGTGGCTCGGCTAAACAAGGACGTGATAGAGAGTTTCAAGCTATCTTACCACTTA
GGGGTAAGATTTTAAATGTAGAAAAAGCAAGATTTGATAAAATCCTGGCAAATGAAGAGATTAAAACTATTATTACAGCT
ATTGGAACTGGAATAGGTGAAGAATTTGATATCTCCAAGGCCAGATATCAACGAATAATAATTATGACAGATGCCGATGT
TGACGGTGCTCATATCAGAACTTTACTATTAACTTTCTTCTACAGATATATGAGGCCTTTAATTGAACACGGTTACGTTT
ATATAGCTCAACCGCCATTATATATGGTTAAGAAGAAGGGTAAACATTACTACACTTTTAATGATCATGAACTAGATAAT
TTATTAGAGGAAATAGGGAAAGAAGGTATTAGTCTTCAAAGATATAAAGGTCTAGGGGAAATGAATCCTACTCAACTTTG
GGAAACAACTATGAATCCAGAAAGTAGAACTGTTTTAAATGTGAATCTACAAGACGCCATTGAAGCCGATAAAATATTTA
CGGTTCTCATGGGTGAGAAGGTCAGTCCTCGACGTAAATTTATCGAAGAAAATGCCCGAGATGTGAGAAATTTGGATGTT
TAA

Upstream 100 bases:

>100_bases
GGAAGAAGAGGATGAAATAGATAATACTTAATAATTAAATTTGATTAAACCGAGCAACTTTTGTGGTATAATATGGTATG
CGAGAAATGGGGGTTTAGTA

Downstream 100 bases:

>100_bases
AAAATATGTTTAATTTTTAGCAGATCTTTTAACAGATTGATAATAAGATAACAAGCTTTGAAAAGAAAAGGAAGGGAAGC
TATTATGAGCGATACTATGC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 640; Mature: 640

Protein sequence:

>640_residues
MEENKFVNEQNYDESQIQVLEGLEAVRKRPGMYIGSTDERGLHHLVHEVVDNSIDEAMAGYCDDIRIKLEPGNKVRVTDN
GRGIPVKDHPKIKKPTLEVIMTYLHAGGKFSNDSYKVSGGLHGVGISVVNGLSKWIRVKVYRDGKVYQQEYEKGYPKTEI
KEIGESDKRGTEIIFLPDEEIFETVEFDYQVLRNRLQQLAFLNRGVSITISDEREDEFKEEHFKYEGGIKSFVEYLNENK
TTLFTPPVYIEGEQEGCKLEVAIQYNDGYQEMIHSYANNINTHEGGTHESGFKISLTRTVNDYARRHNLLKNGDKNLTGE
DIREGITAIISVKVEDPQFEGQTKTKLGNSEVRRIVEGIVSEHLESYLEENPAVGKKIVNKAINASRAREAARKAKELTR
RKNALEVTALPGKLADCTTKDASKSELYLVEGDSAGGSAKQGRDREFQAILPLRGKILNVEKARFDKILANEEIKTIITA
IGTGIGEEFDISKARYQRIIIMTDADVDGAHIRTLLLTFFYRYMRPLIEHGYVYIAQPPLYMVKKKGKHYYTFNDHELDN
LLEEIGKEGISLQRYKGLGEMNPTQLWETTMNPESRTVLNVNLQDAIEADKIFTVLMGEKVSPRRKFIEENARDVRNLDV

Sequences:

>Translated_640_residues
MEENKFVNEQNYDESQIQVLEGLEAVRKRPGMYIGSTDERGLHHLVHEVVDNSIDEAMAGYCDDIRIKLEPGNKVRVTDN
GRGIPVKDHPKIKKPTLEVIMTYLHAGGKFSNDSYKVSGGLHGVGISVVNGLSKWIRVKVYRDGKVYQQEYEKGYPKTEI
KEIGESDKRGTEIIFLPDEEIFETVEFDYQVLRNRLQQLAFLNRGVSITISDEREDEFKEEHFKYEGGIKSFVEYLNENK
TTLFTPPVYIEGEQEGCKLEVAIQYNDGYQEMIHSYANNINTHEGGTHESGFKISLTRTVNDYARRHNLLKNGDKNLTGE
DIREGITAIISVKVEDPQFEGQTKTKLGNSEVRRIVEGIVSEHLESYLEENPAVGKKIVNKAINASRAREAARKAKELTR
RKNALEVTALPGKLADCTTKDASKSELYLVEGDSAGGSAKQGRDREFQAILPLRGKILNVEKARFDKILANEEIKTIITA
IGTGIGEEFDISKARYQRIIIMTDADVDGAHIRTLLLTFFYRYMRPLIEHGYVYIAQPPLYMVKKKGKHYYTFNDHELDN
LLEEIGKEGISLQRYKGLGEMNPTQLWETTMNPESRTVLNVNLQDAIEADKIFTVLMGEKVSPRRKFIEENARDVRNLDV
>Mature_640_residues
MEENKFVNEQNYDESQIQVLEGLEAVRKRPGMYIGSTDERGLHHLVHEVVDNSIDEAMAGYCDDIRIKLEPGNKVRVTDN
GRGIPVKDHPKIKKPTLEVIMTYLHAGGKFSNDSYKVSGGLHGVGISVVNGLSKWIRVKVYRDGKVYQQEYEKGYPKTEI
KEIGESDKRGTEIIFLPDEEIFETVEFDYQVLRNRLQQLAFLNRGVSITISDEREDEFKEEHFKYEGGIKSFVEYLNENK
TTLFTPPVYIEGEQEGCKLEVAIQYNDGYQEMIHSYANNINTHEGGTHESGFKISLTRTVNDYARRHNLLKNGDKNLTGE
DIREGITAIISVKVEDPQFEGQTKTKLGNSEVRRIVEGIVSEHLESYLEENPAVGKKIVNKAINASRAREAARKAKELTR
RKNALEVTALPGKLADCTTKDASKSELYLVEGDSAGGSAKQGRDREFQAILPLRGKILNVEKARFDKILANEEIKTIITA
IGTGIGEEFDISKARYQRIIIMTDADVDGAHIRTLLLTFFYRYMRPLIEHGYVYIAQPPLYMVKKKGKHYYTFNDHELDN
LLEEIGKEGISLQRYKGLGEMNPTQLWETTMNPESRTVLNVNLQDAIEADKIFTVLMGEKVSPRRKFIEENARDVRNLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=605, Percent_Identity=25.2892561983471, Blast_Score=140, Evalue=5e-33,
Organism=Homo sapiens, GI19913406, Length=604, Percent_Identity=24.8344370860927, Blast_Score=139, Evalue=7e-33,
Organism=Escherichia coli, GI48994957, Length=566, Percent_Identity=56.1837455830389, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI1789408, Length=630, Percent_Identity=40.9523809523809, Blast_Score=463, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17535065, Length=610, Percent_Identity=25.7377049180328, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI212645845, Length=595, Percent_Identity=25.3781512605042, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI212645657, Length=127, Percent_Identity=32.2834645669291, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=618, Percent_Identity=26.6990291262136, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI17136538, Length=618, Percent_Identity=25.4045307443366, Blast_Score=151, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72813; Mature: 72813

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEENKFVNEQNYDESQIQVLEGLEAVRKRPGMYIGSTDERGLHHLVHEVVDNSIDEAMAG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCHHHHHHHC
YCDDIRIKLEPGNKVRVTDNGRGIPVKDHPKIKKPTLEVIMTYLHAGGKFSNDSYKVSGG
CCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCC
LHGVGISVVNGLSKWIRVKVYRDGKVYQQEYEKGYPKTEIKEIGESDKRGTEIIFLPDEE
CCCHHHHHHHHHHHHEEEEEEECCHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEECCHH
IFETVEFDYQVLRNRLQQLAFLNRGVSITISDEREDEFKEEHFKYEGGIKSFVEYLNENK
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCC
TTLFTPPVYIEGEQEGCKLEVAIQYNDGYQEMIHSYANNINTHEGGTHESGFKISLTRTV
CEEECCCEEEECCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHH
NDYARRHNLLKNGDKNLTGEDIREGITAIISVKVEDPQFEGQTKTKLGNSEVRRIVEGIV
HHHHHHHHHHHCCCCCCCHHHHHHCCEEEEEEEEECCCCCCCHHHCCCHHHHHHHHHHHH
SEHLESYLEENPAVGKKIVNKAINASRAREAARKAKELTRRKNALEVTALPGKLADCTTK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCC
DASKSELYLVEGDSAGGSAKQGRDREFQAILPLRGKILNVEKARFDKILANEEIKTIITA
CCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCEEECHHHHHHHHHHCCHHHHHHHHH
IGTGIGEEFDISKARYQRIIIMTDADVDGAHIRTLLLTFFYRYMRPLIEHGYVYIAQPPL
HHCCCCCCCCCCHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
YMVKKKGKHYYTFNDHELDNLLEEIGKEGISLQRYKGLGEMNPTQLWETTMNPESRTVLN
EEHHCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHCCCCCCCEEEE
VNLQDAIEADKIFTVLMGEKVSPRRKFIEENARDVRNLDV
ECHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEENKFVNEQNYDESQIQVLEGLEAVRKRPGMYIGSTDERGLHHLVHEVVDNSIDEAMAG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCHHHHHHHC
YCDDIRIKLEPGNKVRVTDNGRGIPVKDHPKIKKPTLEVIMTYLHAGGKFSNDSYKVSGG
CCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCC
LHGVGISVVNGLSKWIRVKVYRDGKVYQQEYEKGYPKTEIKEIGESDKRGTEIIFLPDEE
CCCHHHHHHHHHHHHEEEEEEECCHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEECCHH
IFETVEFDYQVLRNRLQQLAFLNRGVSITISDEREDEFKEEHFKYEGGIKSFVEYLNENK
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCC
TTLFTPPVYIEGEQEGCKLEVAIQYNDGYQEMIHSYANNINTHEGGTHESGFKISLTRTV
CEEECCCEEEECCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHH
NDYARRHNLLKNGDKNLTGEDIREGITAIISVKVEDPQFEGQTKTKLGNSEVRRIVEGIV
HHHHHHHHHHHCCCCCCCHHHHHHCCEEEEEEEEECCCCCCCHHHCCCHHHHHHHHHHHH
SEHLESYLEENPAVGKKIVNKAINASRAREAARKAKELTRRKNALEVTALPGKLADCTTK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCC
DASKSELYLVEGDSAGGSAKQGRDREFQAILPLRGKILNVEKARFDKILANEEIKTIITA
CCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCEEECHHHHHHHHHHCCHHHHHHHHH
IGTGIGEEFDISKARYQRIIIMTDADVDGAHIRTLLLTFFYRYMRPLIEHGYVYIAQPPL
HHCCCCCCCCCCHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
YMVKKKGKHYYTFNDHELDNLLEEIGKEGISLQRYKGLGEMNPTQLWETTMNPESRTVLN
EEHHCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHCCCCCCCEEEE
VNLQDAIEADKIFTVLMGEKVSPRRKFIEENARDVRNLDV
ECHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10427704; 11058132 [H]