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The map label for this gene is rlmB [H]

Identifier: 188527602

GI number: 188527602

Start: 799504

End: 800187

Strand: Reverse

Name: rlmB [H]

Synonym: HPSH_04120

Alternate gene names: 188527602

Gene position: 800187-799504 (Counterclockwise)

Preceding gene: 188527603

Following gene: 188527601

Centisome position: 49.75

GC content: 39.33

Gene sequence:

>684_bases
ATGCAAGCAGTAATTTATGGCAAGCAAGTGATCATGCACATTCTAAACTCTCATCAAGAAAAATTGCAAGAAATCTATCT
TTCTAAAGAAATAGACAAAAAGCTTTTTTTCGCGCTCAAAAAAGCATGCCCTAATATCATCAAAGTGGATAATAAAAAAG
CGCAAAGCTTGGCTAAGGGGGGGAACCATCAAGGGGTTTTAGCTAAGGTGGAACTGCCTTTAGCGGTTTCTTTAAAAGAG
ATTAAAAAAGCTCAAAAACTTTTGGTGCTTTGTGGGATTACGGATGTGGGGAATATTGGGGGTATTTTTAGGAGCGCGTA
TTGTTTAGGAATGGATGGCGTTATTTTAGATTTTGCTAAAGAATTGGCTTATGAGGGGATTGTGCGATCCAGCTTGGGGC
TTATGTATGATTTGCCTTTTAGCGTTGCGCCTAACACGCTGGATTTAATCAATGAATTGAAAACGAGCGGGTTTTTATGT
TTGGGCGCGAGCATGCAAGGCTCTAGTCAAGTAGAAAATCTATCCTTAAAAAAATGCACTCTTTTTTTGGGGAGCGAGCA
TGAGGGGTTGTCTAAAAAAATCCTCGCTAAAATGGACACTATATTAAGCGTAAAAATGCGAAGAGATTTTGATTCGCTCA
ATGTGAGCGTGGCAGCAGGGATCTTAATGGATAAAATCAACTAG

Upstream 100 bases:

>100_bases
AATTAAGGCTAAAATTGAAGCCGCTATGACACAAAAAAACGCTAAAGAGCTTTATTTCCAGCGTTTGTTAGAAGAAAAAA
ATCAATAAAAGGGGTTTAGC

Downstream 100 bases:

>100_bases
GTGGTCAATTGAATGGAACAGCATAAAAAAAGTTTAGAAAATTTAGATCTTACTGATGTTCAAAACATTTCTAAAGATAT
TTCTGGTGCGGCATTGGAAG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MQAVIYGKQVIMHILNSHQEKLQEIYLSKEIDKKLFFALKKACPNIIKVDNKKAQSLAKGGNHQGVLAKVELPLAVSLKE
IKKAQKLLVLCGITDVGNIGGIFRSAYCLGMDGVILDFAKELAYEGIVRSSLGLMYDLPFSVAPNTLDLINELKTSGFLC
LGASMQGSSQVENLSLKKCTLFLGSEHEGLSKKILAKMDTILSVKMRRDFDSLNVSVAAGILMDKIN

Sequences:

>Translated_227_residues
MQAVIYGKQVIMHILNSHQEKLQEIYLSKEIDKKLFFALKKACPNIIKVDNKKAQSLAKGGNHQGVLAKVELPLAVSLKE
IKKAQKLLVLCGITDVGNIGGIFRSAYCLGMDGVILDFAKELAYEGIVRSSLGLMYDLPFSVAPNTLDLINELKTSGFLC
LGASMQGSSQVENLSLKKCTLFLGSEHEGLSKKILAKMDTILSVKMRRDFDSLNVSVAAGILMDKIN
>Mature_227_residues
MQAVIYGKQVIMHILNSHQEKLQEIYLSKEIDKKLFFALKKACPNIIKVDNKKAQSLAKGGNHQGVLAKVELPLAVSLKE
IKKAQKLLVLCGITDVGNIGGIFRSAYCLGMDGVILDFAKELAYEGIVRSSLGLMYDLPFSVAPNTLDLINELKTSGFLC
LGASMQGSSQVENLSLKKCTLFLGSEHEGLSKKILAKMDTILSVKMRRDFDSLNVSVAAGILMDKIN

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=239, Percent_Identity=25.5230125523013, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 24819; Mature: 24819

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAVIYGKQVIMHILNSHQEKLQEIYLSKEIDKKLFFALKKACPNIIKVDNKKAQSLAKG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCHHHHHHHCC
GNHQGVLAKVELPLAVSLKEIKKAQKLLVLCGITDVGNIGGIFRSAYCLGMDGVILDFAK
CCCCCEEEEEECCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHH
ELAYEGIVRSSLGLMYDLPFSVAPNTLDLINELKTSGFLCLGASMQGSSQVENLSLKKCT
HHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHCCCHHHEE
LFLGSEHEGLSKKILAKMDTILSVKMRRDFDSLNVSVAAGILMDKIN
EEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MQAVIYGKQVIMHILNSHQEKLQEIYLSKEIDKKLFFALKKACPNIIKVDNKKAQSLAKG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCHHHHHHHCC
GNHQGVLAKVELPLAVSLKEIKKAQKLLVLCGITDVGNIGGIFRSAYCLGMDGVILDFAK
CCCCCEEEEEECCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHH
ELAYEGIVRSSLGLMYDLPFSVAPNTLDLINELKTSGFLCLGASMQGSSQVENLSLKKCT
HHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHCCCHHHEE
LFLGSEHEGLSKKILAKMDTILSVKMRRDFDSLNVSVAAGILMDKIN
EEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]