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The map label for this gene is hydB [H]

Identifier: 188527519

GI number: 188527519

Start: 709724

End: 711460

Strand: Reverse

Name: hydB [H]

Synonym: HPSH_03705

Alternate gene names: 188527519

Gene position: 711460-709724 (Counterclockwise)

Preceding gene: 188527520

Following gene: 188527518

Centisome position: 44.23

GC content: 43.18

Gene sequence:

>1737_bases
ATGTCAAAAAAAATAGTAGTCGATCCTATCACTAGGATTGAGGGGCATTTAAGGATTGAAGTGATCGTAGATGATGATAA
TGTGATCACTGATGCGTTTTCTTCTTCTACGCTTTTTAGGGGGCTAGAAACGATCATTAAAGGCAGAGATCCACGAGATG
CAGGCTTTATCGCTCAAAGGATTTGCGGGGTATGCACTTATTCGCATTATAAGGCCGGTATCACGGCGGTAGAAAACGCT
CTAGGCATCACCCCCCCATTAAACGCGCAATTGGTGCGATCTTTGATGAACATGGCGTTGCTTTTTCACGATCATGTGGT
GCATTTCTATACTTTGCATGGGCTTGATTGGTGCGATATTATGAGCGCTTTAAAAGCCGATCCCATTCAAGCGGCAAAAC
TTTCTTTCAAATACAGCCCTTATCCTATCAATACCGGTGCCGGCGAATTAAAAGCGGTTCAAAAACGCTTGAGCGATTTC
GCTAAAGGTGGATCGTTAGGGCCTTTTAATAACGGCTATTACGGGCATAAAACCTATCGTTTAAGCCCAGAACAAAATTT
AATCGTCCTAAGCCACTATCTCAAGCTTTTAGAAATCCAAAGGGAAGCGGCGAAAATGACCGCTATTTTTGGGGCCAAAC
AGCCTCACCCACAAAGCCTAACGGTGGGGGGTGTTACGAGCGTTATGGATATATTGGATCCGACGAGATTGGCTGAATGG
AAGAGCAAGTTTGAAGTGGTGGCTCATTTCATCAACCATGCTTACTACCCGGATTTAGTGATGGCGGGCGAAATGTTCGC
TAATGAGCCGTCCGTTATTAAAGGCTGTGGCTTGAGGAACTTTATCGCTTATGAAGAAGTGTTGCTTGGGAAGGATAAAT
ACCTTTTGAGTAGTGGGGTGGTGCTTGATGGGGATATTTCCAAACTGCACCCCATTGATGAAAGTTTGATTAAAGAAGAA
GTTACGCATTCTTGGTATCAATACGAAGACACTAAAGAAGTGCAACTCCACCCTTATGACGGGCAAACTAACCCGCATTA
TACCGGTTTAAAAGACGGCGAGAGCGTGGGGATTGAAAATAAAATCATCCCTGCTAAAGTGCTTGACACTCAAAATAAAT
ATTCTTGGATAAAATCGCCCAGATACGATAGTAAGCCCATGGAAGTAGGCCCTTTAAGTTCCGTAGTGGTAGGTTTGGCG
GCGAAAAACCCTTATGTTACTGAAGTGGCTACTAAGTTTTTAAAAGACACTAAACTGCCTTTAGAAGCGTTGTTTTCAAC
GCTTGGGCGCACGGCTGCAAGGTGTATTGAAGCTAAAACTATCGCTGATAATGGCCTTTTGGCGTTTGATGCGTTAGTGG
AAAATCTAAAAAGCGATCAAAGCACTTGCGCTCCTTATTACATTGATAAAAATCAAGAATATAAAGGGCGCTACATTGGT
CAAGTGCCAAGGGGCATGCTAAGCCATTGGGTGCGTATTAAAAACGGCGTGGTGGAAAATTATCAAGCGGTGGTGCCTTC
TACTTGGAATGCAGGGCCTAGAGATTCTAAAAATCAAAGGGGGGCTTATGAAATGAGCTTGATTGGCACTAAAATCGCTG
ATTTAACCCAGCCTTTAGAAATCATTAGGACTATCCATTCTTTTGATCCATGCATCGCATGCTCGGTGCATGTGATGGAT
TTTAAAGGGCAGTCTTTAAACGAGTTTAAAGTAGAGCCTAATTTCGCTAAATTCTAA

Upstream 100 bases:

>100_bases
GTAGGCACGACTTTATTGAGTGCAACCGCTATTGGCATTGTCGCGCATGCACTCCTTTCTAAAGCGATCAAAAACAAAGA
GTAATGAATAAGGGATTAAC

Downstream 100 bases:

>100_bases
AAAGGGTTACGCATGGATAAAATGAATAAGGTCGTTTTACACAAAGAATATTCAGGTTTTGTGCGCTTTTTCCATTGGGT
TAGGGCTTTGAGTATTTTCG

Product: quinone-reactive Ni/Fe hydrogenase HydB

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MSKKIVVDPITRIEGHLRIEVIVDDDNVITDAFSSSTLFRGLETIIKGRDPRDAGFIAQRICGVCTYSHYKAGITAVENA
LGITPPLNAQLVRSLMNMALLFHDHVVHFYTLHGLDWCDIMSALKADPIQAAKLSFKYSPYPINTGAGELKAVQKRLSDF
AKGGSLGPFNNGYYGHKTYRLSPEQNLIVLSHYLKLLEIQREAAKMTAIFGAKQPHPQSLTVGGVTSVMDILDPTRLAEW
KSKFEVVAHFINHAYYPDLVMAGEMFANEPSVIKGCGLRNFIAYEEVLLGKDKYLLSSGVVLDGDISKLHPIDESLIKEE
VTHSWYQYEDTKEVQLHPYDGQTNPHYTGLKDGESVGIENKIIPAKVLDTQNKYSWIKSPRYDSKPMEVGPLSSVVVGLA
AKNPYVTEVATKFLKDTKLPLEALFSTLGRTAARCIEAKTIADNGLLAFDALVENLKSDQSTCAPYYIDKNQEYKGRYIG
QVPRGMLSHWVRIKNGVVENYQAVVPSTWNAGPRDSKNQRGAYEMSLIGTKIADLTQPLEIIRTIHSFDPCIACSVHVMD
FKGQSLNEFKVEPNFAKF

Sequences:

>Translated_578_residues
MSKKIVVDPITRIEGHLRIEVIVDDDNVITDAFSSSTLFRGLETIIKGRDPRDAGFIAQRICGVCTYSHYKAGITAVENA
LGITPPLNAQLVRSLMNMALLFHDHVVHFYTLHGLDWCDIMSALKADPIQAAKLSFKYSPYPINTGAGELKAVQKRLSDF
AKGGSLGPFNNGYYGHKTYRLSPEQNLIVLSHYLKLLEIQREAAKMTAIFGAKQPHPQSLTVGGVTSVMDILDPTRLAEW
KSKFEVVAHFINHAYYPDLVMAGEMFANEPSVIKGCGLRNFIAYEEVLLGKDKYLLSSGVVLDGDISKLHPIDESLIKEE
VTHSWYQYEDTKEVQLHPYDGQTNPHYTGLKDGESVGIENKIIPAKVLDTQNKYSWIKSPRYDSKPMEVGPLSSVVVGLA
AKNPYVTEVATKFLKDTKLPLEALFSTLGRTAARCIEAKTIADNGLLAFDALVENLKSDQSTCAPYYIDKNQEYKGRYIG
QVPRGMLSHWVRIKNGVVENYQAVVPSTWNAGPRDSKNQRGAYEMSLIGTKIADLTQPLEIIRTIHSFDPCIACSVHVMD
FKGQSLNEFKVEPNFAKF
>Mature_577_residues
SKKIVVDPITRIEGHLRIEVIVDDDNVITDAFSSSTLFRGLETIIKGRDPRDAGFIAQRICGVCTYSHYKAGITAVENAL
GITPPLNAQLVRSLMNMALLFHDHVVHFYTLHGLDWCDIMSALKADPIQAAKLSFKYSPYPINTGAGELKAVQKRLSDFA
KGGSLGPFNNGYYGHKTYRLSPEQNLIVLSHYLKLLEIQREAAKMTAIFGAKQPHPQSLTVGGVTSVMDILDPTRLAEWK
SKFEVVAHFINHAYYPDLVMAGEMFANEPSVIKGCGLRNFIAYEEVLLGKDKYLLSSGVVLDGDISKLHPIDESLIKEEV
THSWYQYEDTKEVQLHPYDGQTNPHYTGLKDGESVGIENKIIPAKVLDTQNKYSWIKSPRYDSKPMEVGPLSSVVVGLAA
KNPYVTEVATKFLKDTKLPLEALFSTLGRTAARCIEAKTIADNGLLAFDALVENLKSDQSTCAPYYIDKNQEYKGRYIGQ
VPRGMLSHWVRIKNGVVENYQAVVPSTWNAGPRDSKNQRGAYEMSLIGTKIADLTQPLEIIRTIHSFDPCIACSVHVMDF
KGQSLNEFKVEPNFAKF

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=595, Percent_Identity=41.5126050420168, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI1789368, Length=595, Percent_Identity=41.0084033613445, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1789076, Length=96, Percent_Identity=32.2916666666667, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 64385; Mature: 64253

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKIVVDPITRIEGHLRIEVIVDDDNVITDAFSSSTLFRGLETIIKGRDPRDAGFIAQR
CCCCEEECCHHHCCCEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ICGVCTYSHYKAGITAVENALGITPPLNAQLVRSLMNMALLFHDHVVHFYTLHGLDWCDI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHH
MSALKADPIQAAKLSFKYSPYPINTGAGELKAVQKRLSDFAKGGSLGPFNNGYYGHKTYR
HHHHCCCCCCHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
LSPEQNLIVLSHYLKLLEIQREAAKMTAIFGAKQPHPQSLTVGGVTSVMDILDPTRLAEW
ECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHCHHHHHHH
KSKFEVVAHFINHAYYPDLVMAGEMFANEPSVIKGCGLRNFIAYEEVLLGKDKYLLSSGV
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHCCCHHHHCCCC
VLDGDISKLHPIDESLIKEEVTHSWYQYEDTKEVQLHPYDGQTNPHYTGLKDGESVGIEN
EEECCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
KIIPAKVLDTQNKYSWIKSPRYDSKPMEVGPLSSVVVGLAAKNPYVTEVATKFLKDTKLP
CCCCHHHHCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
LEALFSTLGRTAARCIEAKTIADNGLLAFDALVENLKSDQSTCAPYYIDKNQEYKGRYIG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHH
QVPRGMLSHWVRIKNGVVENYQAVVPSTWNAGPRDSKNQRGAYEMSLIGTKIADLTQPLE
HCHHHHHHHHHHHHCCHHHCCCEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
IIRTIHSFDPCIACSVHVMDFKGQSLNEFKVEPNFAKF
HHHHHHHCCCCCEEEEEEEECCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
SKKIVVDPITRIEGHLRIEVIVDDDNVITDAFSSSTLFRGLETIIKGRDPRDAGFIAQR
CCCEEECCHHHCCCEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ICGVCTYSHYKAGITAVENALGITPPLNAQLVRSLMNMALLFHDHVVHFYTLHGLDWCDI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHH
MSALKADPIQAAKLSFKYSPYPINTGAGELKAVQKRLSDFAKGGSLGPFNNGYYGHKTYR
HHHHCCCCCCHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
LSPEQNLIVLSHYLKLLEIQREAAKMTAIFGAKQPHPQSLTVGGVTSVMDILDPTRLAEW
ECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHCHHHHHHH
KSKFEVVAHFINHAYYPDLVMAGEMFANEPSVIKGCGLRNFIAYEEVLLGKDKYLLSSGV
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHCCCHHHHCCCC
VLDGDISKLHPIDESLIKEEVTHSWYQYEDTKEVQLHPYDGQTNPHYTGLKDGESVGIEN
EEECCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
KIIPAKVLDTQNKYSWIKSPRYDSKPMEVGPLSSVVVGLAAKNPYVTEVATKFLKDTKLP
CCCCHHHHCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
LEALFSTLGRTAARCIEAKTIADNGLLAFDALVENLKSDQSTCAPYYIDKNQEYKGRYIG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHH
QVPRGMLSHWVRIKNGVVENYQAVVPSTWNAGPRDSKNQRGAYEMSLIGTKIADLTQPLE
HCHHHHHHHHHHHHCCHHHCCCEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
IIRTIHSFDPCIACSVHVMDFKGQSLNEFKVEPNFAKF
HHHHHHHCCCCCEEEEEEEECCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]