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The map label for this gene is hydC [H]

Identifier: 188527518

GI number: 188527518

Start: 709037

End: 709711

Strand: Reverse

Name: hydC [H]

Synonym: HPSH_03700

Alternate gene names: 188527518

Gene position: 709711-709037 (Counterclockwise)

Preceding gene: 188527519

Following gene: 188527517

Centisome position: 44.12

GC content: 40.15

Gene sequence:

>675_bases
ATGGATAAAATGAATAAGGTCGTTTTACACAAAGAATATTCAGGTTTTGTGCGCTTTTTCCATTGGGTTAGGGCTTTGAG
TATTTTCGCTTTAATCGCTACAGGGTTTTACATCGCTTACCCTTTTTTGCAGCCTAATTCCAGTTTTTATAAAGGGGTGT
ATCTTTTACAAGCTTATGTGCGCTCTTTTCATGTCATGTTTGGGTTTTTACTCATTAGTGCATTAATCTTTAGAACCTAT
CTTTTTTTCACTAAAGAAAGCTTGATGGAACGCAGGAGTTTTAGCCAACTTTTAAGCCCAAAAGCCTGGATTGATCAGAT
GAAAGCGTATTTTCTTATCAGCGGCAAGACTCACACTAAAGGGTTGTATAACCCTATCCAGCTCGTGGCTTATTCCACTT
TGGTTGTTTTGATAGTGTTGATGAGCTTGAGCGGGGTGGTGCTGTATTATAATGTCTATCATGCGGGGCTTGGAGCGTTT
TTAGCGAGCGCTTTTAAGTGGTTTGAAGCGCTTTGTGGGGGGTTAGCGAATGTTCGTTTTATCCACCACTTGGCGACTTG
GGGGTTTATTTTGTTTGTCCCTGTGCATGTTTATATGGTGTTTTTCCATTCTATCAGGTATGAAAGTTCGGGGGCGGATT
CTATGATTAATGGCTATGGTTATACCAAAGAATGA

Upstream 100 bases:

>100_bases
ATGCATCGCATGCTCGGTGCATGTGATGGATTTTAAAGGGCAGTCTTTAAACGAGTTTAAAGTAGAGCCTAATTTCGCTA
AATTCTAAAAAGGGTTACGC

Downstream 100 bases:

>100_bases
GTCAAAAAATCCTAATTCTAGGCATTGGCAATATCCTTTTTGGCGATGAAGGGATTGGGGTGCATTTAGCCCACTACCTC
AAAAGAAATTTTTCTTTTTT

Product: quinone-reactive Ni/Fe hydrogenase, cytochrome b subunit (hydC)

Products: oxidized ferredoxin; H2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MDKMNKVVLHKEYSGFVRFFHWVRALSIFALIATGFYIAYPFLQPNSSFYKGVYLLQAYVRSFHVMFGFLLISALIFRTY
LFFTKESLMERRSFSQLLSPKAWIDQMKAYFLISGKTHTKGLYNPIQLVAYSTLVVLIVLMSLSGVVLYYNVYHAGLGAF
LASAFKWFEALCGGLANVRFIHHLATWGFILFVPVHVYMVFFHSIRYESSGADSMINGYGYTKE

Sequences:

>Translated_224_residues
MDKMNKVVLHKEYSGFVRFFHWVRALSIFALIATGFYIAYPFLQPNSSFYKGVYLLQAYVRSFHVMFGFLLISALIFRTY
LFFTKESLMERRSFSQLLSPKAWIDQMKAYFLISGKTHTKGLYNPIQLVAYSTLVVLIVLMSLSGVVLYYNVYHAGLGAF
LASAFKWFEALCGGLANVRFIHHLATWGFILFVPVHVYMVFFHSIRYESSGADSMINGYGYTKE
>Mature_224_residues
MDKMNKVVLHKEYSGFVRFFHWVRALSIFALIATGFYIAYPFLQPNSSFYKGVYLLQAYVRSFHVMFGFLLISALIFRTY
LFFTKESLMERRSFSQLLSPKAWIDQMKAYFLISGKTHTKGLYNPIQLVAYSTLVVLIVLMSLSGVVLYYNVYHAGLGAF
LASAFKWFEALCGGLANVRFIHHLATWGFILFVPVHVYMVFFHSIRYESSGADSMINGYGYTKE

Specific function: B-type cytochrome that interacts with quinones and anchor the hydrogenase to the membrane [H]

COG id: COG1969

COG function: function code C; Ni,Fe-hydrogenase I cytochrome b subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupC/hyaC/hydC family [H]

Homologues:

Organism=Escherichia coli, GI1787208, Length=222, Percent_Identity=24.7747747747748, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR000516 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: NA

Molecular weight: Translated: 25837; Mature: 25837

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKMNKVVLHKEYSGFVRFFHWVRALSIFALIATGFYIAYPFLQPNSSFYKGVYLLQAYV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
RSFHVMFGFLLISALIFRTYLFFTKESLMERRSFSQLLSPKAWIDQMKAYFLISGKTHTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCC
GLYNPIQLVAYSTLVVLIVLMSLSGVVLYYNVYHAGLGAFLASAFKWFEALCGGLANVRF
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHHLATWGFILFVPVHVYMVFFHSIRYESSGADSMINGYGYTKE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCC
>Mature Secondary Structure
MDKMNKVVLHKEYSGFVRFFHWVRALSIFALIATGFYIAYPFLQPNSSFYKGVYLLQAYV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
RSFHVMFGFLLISALIFRTYLFFTKESLMERRSFSQLLSPKAWIDQMKAYFLISGKTHTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCC
GLYNPIQLVAYSTLVVLIVLMSLSGVVLYYNVYHAGLGAFLASAFKWFEALCGGLANVRF
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHHLATWGFILFVPVHVYMVFFHSIRYESSGADSMINGYGYTKE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced ferredoxin; 2 H+ [C]

Specific reaction: Reduced ferredoxin + 2 H+ = 2 oxidized ferredoxin + H2 [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]