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The map label for this gene is cca [H]

Identifier: 188527511

GI number: 188527511

Start: 702902

End: 704068

Strand: Reverse

Name: cca [H]

Synonym: HPSH_03665

Alternate gene names: 188527511

Gene position: 704068-702902 (Counterclockwise)

Preceding gene: 188527513

Following gene: 188527510

Centisome position: 43.77

GC content: 36.42

Gene sequence:

>1167_bases
GTGTTTGAGTTAGACAAAGAATTACAAGAATTGTTTGACATTTATGAAAAATCCTCTCATGAGCTTTACTTGGTAGGGGG
GTGCGTGCGCGATTGTTTGATGGGAATTACCCCTAAAGACTACGATTTAACTTCAAACGCTTTAGTCAATGAAAGTAAAG
AACTTCTTTTAAAACACCATTTTAGGGTGCTAGAAACCGGCATCAAGCATGGCACGATCACGGCTCTTAAAAACAATCAA
AGCTATGAAATCACAACCTTTAGAATTGAAAAAGGGCATATCAAGCACCGAAAGCCTAAAGAATTGGTTTTTAGCGCTCG
TTTAACAGACGATTTAAAGCGGCGCGATTTTAGCATGAATGCGATCGCTTATAGCCCTACAAAAGGGGTTATTGATCCTT
TTAAGGGGCAAAATGCGATTGAAAATCAAATAATTGAATGCGTGGGGGGAGCGCGATTGAGGTTTTTTGAGGACGCTTTA
AGGATTTTAAGAGCGTTGCGATTTAGCGCGATTTTAGGCTTTAAGATAGCACCAAGCACTAAAGAAGCGATTTTTGCGTG
TAAGGATTTGTTAAAACATCTTTCTAAAGAGCGCTTACAAAGTGAATTGAATAAGTTTCTTATGGGGAAAAACGCCTATG
AAGTGGCTAAAGAGTATCAAGAAATTTTAGAGTTGGTTACTCAAGAGAAAATAGAAAATTTAGGGTTTTTAAAAAACGCG
CCTTTCAATCTGGGATTAAGATTGTTAGGGTTTTTTAAACATCAAAAAAGTTTAGAAAATTTACGCTACCCTAAAAAAAC
TTGTGTTTTATTTTTAAAAGCTAAAGAATGCCATAAAGATTTTTTAAATATTCATAACAAAACAGGATTAAAATTTTTAT
TGAAAAACTACGATTTAGAGCCTTTTAATTTGGCTTTAGATTTTTATGCGCTCAAAAACCCCAAATGCGCTTTAAAAATT
AAAAGCTTGTTAAAAGAAATATTCAACGCTAACGAGCCTTTTAAGAAAGAACACCTAGCCCTTAAGGGTGGGACGCTTCA
AAACTTGGGCTACCGGCGCCAAAAAATCGGCGAAATCTTGAACGCATGCCTAAATTTAGTCATCGCTCACCCTGAAAATA
ATTCTTTAGAACGCTTGGTGGAGTGGGTGAGGGATAGGGGTTATTGA

Upstream 100 bases:

>100_bases
CCCGCCGCTAAAACTGATTGCGCAAATTTCTTGTTCCATCAAAATTCCTTAAAATTCTAAAAAGATATAATAACACAAAG
AAATTTTAAGAGTAAAAAGC

Downstream 100 bases:

>100_bases
CTCGTTTTTTTTGCAATAATAACTGCGCCTTAAAAAATACAAAAACGGAGGTTTAATAATGCCAAGTCATGTTAATACCA
TAGTTTTGCATGGAAAAAGC

Product: poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MFELDKELQELFDIYEKSSHELYLVGGCVRDCLMGITPKDYDLTSNALVNESKELLLKHHFRVLETGIKHGTITALKNNQ
SYEITTFRIEKGHIKHRKPKELVFSARLTDDLKRRDFSMNAIAYSPTKGVIDPFKGQNAIENQIIECVGGARLRFFEDAL
RILRALRFSAILGFKIAPSTKEAIFACKDLLKHLSKERLQSELNKFLMGKNAYEVAKEYQEILELVTQEKIENLGFLKNA
PFNLGLRLLGFFKHQKSLENLRYPKKTCVLFLKAKECHKDFLNIHNKTGLKFLLKNYDLEPFNLALDFYALKNPKCALKI
KSLLKEIFNANEPFKKEHLALKGGTLQNLGYRRQKIGEILNACLNLVIAHPENNSLERLVEWVRDRGY

Sequences:

>Translated_388_residues
MFELDKELQELFDIYEKSSHELYLVGGCVRDCLMGITPKDYDLTSNALVNESKELLLKHHFRVLETGIKHGTITALKNNQ
SYEITTFRIEKGHIKHRKPKELVFSARLTDDLKRRDFSMNAIAYSPTKGVIDPFKGQNAIENQIIECVGGARLRFFEDAL
RILRALRFSAILGFKIAPSTKEAIFACKDLLKHLSKERLQSELNKFLMGKNAYEVAKEYQEILELVTQEKIENLGFLKNA
PFNLGLRLLGFFKHQKSLENLRYPKKTCVLFLKAKECHKDFLNIHNKTGLKFLLKNYDLEPFNLALDFYALKNPKCALKI
KSLLKEIFNANEPFKKEHLALKGGTLQNLGYRRQKIGEILNACLNLVIAHPENNSLERLVEWVRDRGY
>Mature_388_residues
MFELDKELQELFDIYEKSSHELYLVGGCVRDCLMGITPKDYDLTSNALVNESKELLLKHHFRVLETGIKHGTITALKNNQ
SYEITTFRIEKGHIKHRKPKELVFSARLTDDLKRRDFSMNAIAYSPTKGVIDPFKGQNAIENQIIECVGGARLRFFEDAL
RILRALRFSAILGFKIAPSTKEAIFACKDLLKHLSKERLQSELNKFLMGKNAYEVAKEYQEILELVTQEKIENLGFLKNA
PFNLGLRLLGFFKHQKSLENLRYPKKTCVLFLKAKECHKDFLNIHNKTGLKFLLKNYDLEPFNLALDFYALKNPKCALKI
KSLLKEIFNANEPFKKEHLALKGGTLQNLGYRRQKIGEILNACLNLVIAHPENNSLERLVEWVRDRGY

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=209, Percent_Identity=34.9282296650718, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI87081691, Length=240, Percent_Identity=31.6666666666667, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789436, Length=197, Percent_Identity=31.4720812182741, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71995920, Length=223, Percent_Identity=36.7713004484305, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321016, Length=260, Percent_Identity=24.2307692307692, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI281360112, Length=209, Percent_Identity=34.9282296650718, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21357337, Length=209, Percent_Identity=34.9282296650718, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 44822; Mature: 44822

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFELDKELQELFDIYEKSSHELYLVGGCVRDCLMGITPKDYDLTSNALVNESKELLLKHH
CCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
FRVLETGIKHGTITALKNNQSYEITTFRIEKGHIKHRKPKELVFSARLTDDLKRRDFSMN
HHHHHHCCCCCEEEEEECCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
AIAYSPTKGVIDPFKGQNAIENQIIECVGGARLRFFEDALRILRALRFSAILGFKIAPST
EEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEECCCH
KEAIFACKDLLKHLSKERLQSELNKFLMGKNAYEVAKEYQEILELVTQEKIENLGFLKNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PFNLGLRLLGFFKHQKSLENLRYPKKTCVLFLKAKECHKDFLNIHNKTGLKFLLKNYDLE
CHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEHHHHHHHHHHCCCCCHHHHHHHCCCCC
PFNLALDFYALKNPKCALKIKSLLKEIFNANEPFKKEHLALKGGTLQNLGYRRQKIGEIL
HHHEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHEECCCCHHHHHHHHHHHHHHH
NACLNLVIAHPENNSLERLVEWVRDRGY
HHHHHHEEECCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MFELDKELQELFDIYEKSSHELYLVGGCVRDCLMGITPKDYDLTSNALVNESKELLLKHH
CCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
FRVLETGIKHGTITALKNNQSYEITTFRIEKGHIKHRKPKELVFSARLTDDLKRRDFSMN
HHHHHHCCCCCEEEEEECCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
AIAYSPTKGVIDPFKGQNAIENQIIECVGGARLRFFEDALRILRALRFSAILGFKIAPST
EEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEECCCH
KEAIFACKDLLKHLSKERLQSELNKFLMGKNAYEVAKEYQEILELVTQEKIENLGFLKNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PFNLGLRLLGFFKHQKSLENLRYPKKTCVLFLKAKECHKDFLNIHNKTGLKFLLKNYDLE
CHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEHHHHHHHHHHCCCCCHHHHHHHCCCCC
PFNLALDFYALKNPKCALKIKSLLKEIFNANEPFKKEHLALKGGTLQNLGYRRQKIGEIL
HHHEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHEECCCCHHHHHHHHHHHHHHH
NACLNLVIAHPENNSLERLVEWVRDRGY
HHHHHHEEECCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA