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The map label for this gene is hphIAM [H]

Identifier: 188527268

GI number: 188527268

Start: 458530

End: 459576

Strand: Direct

Name: hphIAM [H]

Synonym: HPSH_02375

Alternate gene names: 188527268

Gene position: 458530-459576 (Clockwise)

Preceding gene: 188527267

Following gene: 188527269

Centisome position: 28.51

GC content: 36.87

Gene sequence:

>1047_bases
TTGTATAAGGTAGCAGATATTTTTTGTGGTGCTGGAGGATTGAGCTATGGCTTTTCTATGCACCCTTATTTTGAATTGAT
ATGGGCTAACGATATAGACAAGGATGCTATTTTAAGCTATCAAGCCAATCATAAAGAAACGCAAACCATTTTATGTGATA
TTATGCAACTTAATTGCCACAACTTACCATGCGTTTCAATTGATATTCTACTAGGCGGACCACCATGCCAGAGCTATTCT
ACCCTTGGCAAAAGAAAAATGGATGAAAAAGCGAATCTGTTTAAAGAATATTTGCGGCTTTTAGATTTAGTAAAACCAAA
AATGTTTGTCTTTGAAAATGTGGTGGGTTTAATGTCTATGCAAAAAGGGCAATTGTTCAAACAAATTTGTAACGCTTTTA
AAGAGAGAGGTTATGTTTTAGAGCATGCCATTTTAAACGCCCTAGATTATGGTGTGCCTCAAATAAGAGAGCGAGTGATT
TTAGTGGGTGTGCTTAAAAGTTTTAAACAAAAATTCCACTTCCCCAAACCCATAAAAACGCATTTTTCTCTAAAAGACGC
TTTAGGGGATTTACCACCTATTCAAAGCGGTGAAAATGGTGATGCTTTAGGTTATCTTAAAAATGCAGATAATGTTTTTT
TGGAATTTGTGCGAAATTCTAAAGAATTAAGCGAGCATAGCAGTCCTAAAAATAATGAGAAACTTATAAAAATCATGCAA
ATGCTAAAAGACGGACAGAGTAAAGATGATTTGCCCAAAAATTTACGCCCCAAAAGTGGTTATACTAATACCTATGCCAA
AATGTGGTGGGAAAAACCAGCCCCCACCATTACAAGAAATTTTTCTACTCCAAGCAGTTCTAGGTGTATCCATCCAAGAG
ACTCTAGAGCGTTAAGCGTTAGAGAGGGAGCAAGATTGCAAAGCTTTCCTGATAATTATAAATTCTGTGGGAGCACTAGC
GCTAAAAGATTGCAAATTGGCAATGCCGTGCCGCCTTTATTGAGTGTAGCGCTCGCTCATGCGGTTTTTGATTTTTTAAA
GGGGTAA

Upstream 100 bases:

>100_bases
AAAAGATTGAGGAATAGTCTTAGCCTAACCTCAAAAAAAGAACTTTAAGTTTTTACTCCATTTAAAAAGTGTGGGTTTAG
ATGAAAGGAAAAAATAAAAC

Downstream 100 bases:

>100_bases
AAATGTTTGATAATAACGATTTTAAAGGTTATAGAAATTTATTGGGTTTTAATTCTCAAAATACATTTAAGGAATTTTTA
GGCGCTAAAGACATACAACC

Product: type II DNA modification enzyme (methyltransferase)

Products: NA

Alternate protein names: M.HphIA; Cytosine-specific methyltransferase HphIA; M.Hphi(C) [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MYKVADIFCGAGGLSYGFSMHPYFELIWANDIDKDAILSYQANHKETQTILCDIMQLNCHNLPCVSIDILLGGPPCQSYS
TLGKRKMDEKANLFKEYLRLLDLVKPKMFVFENVVGLMSMQKGQLFKQICNAFKERGYVLEHAILNALDYGVPQIRERVI
LVGVLKSFKQKFHFPKPIKTHFSLKDALGDLPPIQSGENGDALGYLKNADNVFLEFVRNSKELSEHSSPKNNEKLIKIMQ
MLKDGQSKDDLPKNLRPKSGYTNTYAKMWWEKPAPTITRNFSTPSSSRCIHPRDSRALSVREGARLQSFPDNYKFCGSTS
AKRLQIGNAVPPLLSVALAHAVFDFLKG

Sequences:

>Translated_348_residues
MYKVADIFCGAGGLSYGFSMHPYFELIWANDIDKDAILSYQANHKETQTILCDIMQLNCHNLPCVSIDILLGGPPCQSYS
TLGKRKMDEKANLFKEYLRLLDLVKPKMFVFENVVGLMSMQKGQLFKQICNAFKERGYVLEHAILNALDYGVPQIRERVI
LVGVLKSFKQKFHFPKPIKTHFSLKDALGDLPPIQSGENGDALGYLKNADNVFLEFVRNSKELSEHSSPKNNEKLIKIMQ
MLKDGQSKDDLPKNLRPKSGYTNTYAKMWWEKPAPTITRNFSTPSSSRCIHPRDSRALSVREGARLQSFPDNYKFCGSTS
AKRLQIGNAVPPLLSVALAHAVFDFLKG
>Mature_348_residues
MYKVADIFCGAGGLSYGFSMHPYFELIWANDIDKDAILSYQANHKETQTILCDIMQLNCHNLPCVSIDILLGGPPCQSYS
TLGKRKMDEKANLFKEYLRLLDLVKPKMFVFENVVGLMSMQKGQLFKQICNAFKERGYVLEHAILNALDYGVPQIRERVI
LVGVLKSFKQKFHFPKPIKTHFSLKDALGDLPPIQSGENGDALGYLKNADNVFLEFVRNSKELSEHSSPKNNEKLIKIMQ
MLKDGQSKDDLPKNLRPKSGYTNTYAKMWWEKPAPTITRNFSTPSSSRCIHPRDSRALSVREGARLQSFPDNYKFCGSTS
AKRLQIGNAVPPLLSVALAHAVFDFLKG

Specific function: This methylase protects the DNA from cleavage by the HphI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI195927037, Length=265, Percent_Identity=23.0188679245283, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4503351, Length=265, Percent_Identity=23.0188679245283, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1788271, Length=221, Percent_Identity=28.5067873303167, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 39290; Mature: 39290

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKVADIFCGAGGLSYGFSMHPYFELIWANDIDKDAILSYQANHKETQTILCDIMQLNCH
CCCHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHEECCCCHHHHHHHHHHHHHCCC
NLPCVSIDILLGGPPCQSYSTLGKRKMDEKANLFKEYLRLLDLVKPKMFVFENVVGLMSM
CCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
QKGQLFKQICNAFKERGYVLEHAILNALDYGVPQIRERVILVGVLKSFKQKFHFPKPIKT
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
HFSLKDALGDLPPIQSGENGDALGYLKNADNVFLEFVRNSKELSEHSSPKNNEKLIKIMQ
HHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
MLKDGQSKDDLPKNLRPKSGYTNTYAKMWWEKPAPTITRNFSTPSSSRCIHPRDSRALSV
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCEEEH
REGARLQSFPDNYKFCGSTSAKRLQIGNAVPPLLSVALAHAVFDFLKG
HCCCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYKVADIFCGAGGLSYGFSMHPYFELIWANDIDKDAILSYQANHKETQTILCDIMQLNCH
CCCHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHEECCCCHHHHHHHHHHHHHCCC
NLPCVSIDILLGGPPCQSYSTLGKRKMDEKANLFKEYLRLLDLVKPKMFVFENVVGLMSM
CCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
QKGQLFKQICNAFKERGYVLEHAILNALDYGVPQIRERVILVGVLKSFKQKFHFPKPIKT
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
HFSLKDALGDLPPIQSGENGDALGYLKNADNVFLEFVRNSKELSEHSSPKNNEKLIKIMQ
HHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
MLKDGQSKDDLPKNLRPKSGYTNTYAKMWWEKPAPTITRNFSTPSSSRCIHPRDSRALSV
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCEEEH
REGARLQSFPDNYKFCGSTSAKRLQIGNAVPPLLSVALAHAVFDFLKG
HCCCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759008 [H]