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The map label for this gene is fliM

Identifier: 188527225

GI number: 188527225

Start: 410761

End: 411825

Strand: Direct

Name: fliM

Synonym: HPSH_02160

Alternate gene names: 188527225

Gene position: 410761-411825 (Clockwise)

Preceding gene: 188527224

Following gene: 188527226

Centisome position: 25.54

GC content: 40.75

Gene sequence:

>1065_bases
ATGGCTGATATTTTAAGCCAAGAAGAAATTGATGCGCTTTTAGAGGTCGTTGATGAAAATGTGGATATTCAAAATGTCCA
AAAAAAAGATATTATCCCGCAGCGCAGCGTAACCCTCTATGATTTCAAACGCCCTAATCGTGTGAGTAAGGAGCAACTGC
GCTCTTTTAGGAGTATCCATGACAAAATGGCTAGGAATCTTTCCAGTCAAGTTTCTTCTATCATGCGTTCTATTGTAGAG
ATTCAGCTCCATAGCGTGGATCAAATGACTTATGGCGAATTTTTGATGAGTTTGCCTAGCCCTACGAGTTTTAATGTCTT
TTCCATGAAGCCTATGGGAGGGACAGGGGTTTTAGAAATCAATCCTAGCATCGCTTTCCCTATGATTGACAGATTATTAG
GGGGCAAGGGGAGCGCGTATGATCAAAACAGGGAGTTTAGCGATATTGAATTGAATTTATTGGATACGATTTTACGCCAA
GTGATGCAAATTTTAAAAGAAGTGTGGTCGCCCGTGGTGGAGATGTTCCCTACCATTGACGCTAAAGAATCCAGCGCGAA
TGTGGTCCAAATCGTCGCTCAAAATGAAATTTCTATCATGGTGGTTTTAGAGATTATTATCGGGCATAGTCGTGGGATGA
TGAATATTTGTTACCCGGTGATTTCCATTGAGAGCATTCTTTCTAAAATGGGGAGTAGGGATTTGATGCTTTCAGAAACG
AATTCCAAAAAGAGCCGTAATAAGGAATTGCAAGCGCTATTGAGCGGGGTGAGCGTGGATATGATGGTGTTTTTGGGCGC
GGTAGAATTGAGTTTGAAAGAAATGCTGGATTTAGATGTGGGGGATACTATCCGGTTGAATAAGATCGCTAATGATGAAG
TGAGCGTGTATGTGCATAAAAAAAAGCGTTATTTAGCGAGCGTGGGGTTTCAAGGGTATAGGAAAACCATTCAAATTAAA
GAAGTGGTCTATAGCGAAAAAGAACGCACTAAAGAAATTTTAGAAATGCTAGAAGAACAGCGCAGAGACAAAGTGGGCGA
TATTATGAAAATAGAAGAAGAGTGA

Upstream 100 bases:

>100_bases
TTGAGCGAGATTAAAGAGATTTTAGGCATCACTGAATCGCGCATTTCTCAAATCATTAAAGAAGTGATTAAAAAGGTGCG
TAAATCCTTAGGAGTGGATC

Downstream 100 bases:

>100_bases
GAAATGCAAGATTTTATTAAAATTTTTATTCAAGAGGTTGTTTCTACTTTAGAAGGGTTAGTGGGTAAGGCTCCGAGCGT
GGGATTGGAAAAAGAAGTTT

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MADILSQEEIDALLEVVDENVDIQNVQKKDIIPQRSVTLYDFKRPNRVSKEQLRSFRSIHDKMARNLSSQVSSIMRSIVE
IQLHSVDQMTYGEFLMSLPSPTSFNVFSMKPMGGTGVLEINPSIAFPMIDRLLGGKGSAYDQNREFSDIELNLLDTILRQ
VMQILKEVWSPVVEMFPTIDAKESSANVVQIVAQNEISIMVVLEIIIGHSRGMMNICYPVISIESILSKMGSRDLMLSET
NSKKSRNKELQALLSGVSVDMMVFLGAVELSLKEMLDLDVGDTIRLNKIANDEVSVYVHKKKRYLASVGFQGYRKTIQIK
EVVYSEKERTKEILEMLEEQRRDKVGDIMKIEEE

Sequences:

>Translated_354_residues
MADILSQEEIDALLEVVDENVDIQNVQKKDIIPQRSVTLYDFKRPNRVSKEQLRSFRSIHDKMARNLSSQVSSIMRSIVE
IQLHSVDQMTYGEFLMSLPSPTSFNVFSMKPMGGTGVLEINPSIAFPMIDRLLGGKGSAYDQNREFSDIELNLLDTILRQ
VMQILKEVWSPVVEMFPTIDAKESSANVVQIVAQNEISIMVVLEIIIGHSRGMMNICYPVISIESILSKMGSRDLMLSET
NSKKSRNKELQALLSGVSVDMMVFLGAVELSLKEMLDLDVGDTIRLNKIANDEVSVYVHKKKRYLASVGFQGYRKTIQIK
EVVYSEKERTKEILEMLEEQRRDKVGDIMKIEEE
>Mature_353_residues
ADILSQEEIDALLEVVDENVDIQNVQKKDIIPQRSVTLYDFKRPNRVSKEQLRSFRSIHDKMARNLSSQVSSIMRSIVEI
QLHSVDQMTYGEFLMSLPSPTSFNVFSMKPMGGTGVLEINPSIAFPMIDRLLGGKGSAYDQNREFSDIELNLLDTILRQV
MQILKEVWSPVVEMFPTIDAKESSANVVQIVAQNEISIMVVLEIIIGHSRGMMNICYPVISIESILSKMGSRDLMLSETN
SKKSRNKELQALLSGVSVDMMVFLGAVELSLKEMLDLDVGDTIRLNKIANDEVSVYVHKKKRYLASVGFQGYRKTIQIKE
VVYSEKERTKEILEMLEEQRRDKVGDIMKIEEE

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=292, Percent_Identity=25, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 40233; Mature: 40102

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADILSQEEIDALLEVVDENVDIQNVQKKDIIPQRSVTLYDFKRPNRVSKEQLRSFRSIH
CCCCCCHHHHHHHHHHHHCCCCHHCCHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
DKMARNLSSQVSSIMRSIVEIQLHSVDQMTYGEFLMSLPSPTSFNVFSMKPMGGTGVLEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEE
NPSIAFPMIDRLLGGKGSAYDQNREFSDIELNLLDTILRQVMQILKEVWSPVVEMFPTID
CCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AKESSANVVQIVAQNEISIMVVLEIIIGHSRGMMNICYPVISIESILSKMGSRDLMLSET
CCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECC
NSKKSRNKELQALLSGVSVDMMVFLGAVELSLKEMLDLDVGDTIRLNKIANDEVSVYVHK
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEH
KKRYLASVGFQGYRKTIQIKEVVYSEKERTKEILEMLEEQRRDKVGDIMKIEEE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ADILSQEEIDALLEVVDENVDIQNVQKKDIIPQRSVTLYDFKRPNRVSKEQLRSFRSIH
CCCCCHHHHHHHHHHHHCCCCHHCCHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
DKMARNLSSQVSSIMRSIVEIQLHSVDQMTYGEFLMSLPSPTSFNVFSMKPMGGTGVLEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEE
NPSIAFPMIDRLLGGKGSAYDQNREFSDIELNLLDTILRQVMQILKEVWSPVVEMFPTID
CCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AKESSANVVQIVAQNEISIMVVLEIIIGHSRGMMNICYPVISIESILSKMGSRDLMLSET
CCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECC
NSKKSRNKELQALLSGVSVDMMVFLGAVELSLKEMLDLDVGDTIRLNKIANDEVSVYVHK
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEH
KKRYLASVGFQGYRKTIQIKEVVYSEKERTKEILEMLEEQRRDKVGDIMKIEEE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8755894; 9665876; 8529894 [H]