Click here to switch to the map view.

The map label for this gene is fliA

Identifier: 188527224

GI number: 188527224

Start: 410016

End: 410768

Strand: Direct

Name: fliA

Synonym: HPSH_02155

Alternate gene names: 188527224

Gene position: 410016-410768 (Clockwise)

Preceding gene: 188527223

Following gene: 188527225

Centisome position: 25.49

GC content: 39.04

Gene sequence:

>753_bases
ATGCCCAAAGAAATTCAAAAAACTCAAACAAGCGAAACAAGCGAAAAAAATATAGAAAAGGTTTTGAACGCCTATGATAA
GCAGCAACACCACCATCAAGACGCGCTCGCTATCCAGTATTTACCCGCCGTGCGCGCTATGGCGTTTCGTTTAAAAGAGC
GCTTGCCCAGTTCTATTGATTTTAACGATCTGGTTTCTATTGGCACTGAAGAATTGATCAAATTAGCCAGGCGTTATGAG
AGTGCGTTAAACGATTCTTTTTGGGGGTATGCTAAAACTCGTGTCAATGGGGCGATGTTAGATTATTTGCGCTCTTTAGA
TGTGATTTCTCGCTCCAACAGAAAGCTGATTAAAAGCATTGATATTGAAATCACCAAACACCTTAATGAGCATGGGAAAG
AGCCTAGCGATGCGTATTTAGCGGAAGTTTTAGGCGAAAATATTGAAAAAATTAAAGAAGCCAAAACGGCTTCAGATATT
TATGCGTTAGTGCCAATAGACGAACAATTCAACGCGATTGAGCAAGATGAAATCACTAAAAAAATTGAAGCAGAAGAATT
GTTAGAGCATGTCCAAAAAGCGCTGAATCAAATGAGCGAAAGAGAGCAAATCCTTATCCAGCTTTATTACTTTGAAGAGT
TGAATTTGAGCGAGATTAAAGAGATTTTAGGCATCACTGAATCGCGCATTTCTCAAATCATTAAAGAAGTGATTAAAAAG
GTGCGTAAATCCTTAGGAGTGGATCATGGCTGA

Upstream 100 bases:

>100_bases
AGCTTGCTTCGTTTCAACCATGAGTTTAAGAACAGAGAAAAAGAAGTGGCTAGTATTTTAGAATACATTAGAAGTTATGA
TTTTGATGATGGAAAATAGA

Downstream 100 bases:

>100_bases
TATTTTAAGCCAAGAAGAAATTGATGCGCTTTTAGAGGTCGTTGATGAAAATGTGGATATTCAAAATGTCCAAAAAAAAG
ATATTATCCCGCAGCGCAGC

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MPKEIQKTQTSETSEKNIEKVLNAYDKQQHHHQDALAIQYLPAVRAMAFRLKERLPSSIDFNDLVSIGTEELIKLARRYE
SALNDSFWGYAKTRVNGAMLDYLRSLDVISRSNRKLIKSIDIEITKHLNEHGKEPSDAYLAEVLGENIEKIKEAKTASDI
YALVPIDEQFNAIEQDEITKKIEAEELLEHVQKALNQMSEREQILIQLYYFEELNLSEIKEILGITESRISQIIKEVIKK
VRKSLGVDHG

Sequences:

>Translated_250_residues
MPKEIQKTQTSETSEKNIEKVLNAYDKQQHHHQDALAIQYLPAVRAMAFRLKERLPSSIDFNDLVSIGTEELIKLARRYE
SALNDSFWGYAKTRVNGAMLDYLRSLDVISRSNRKLIKSIDIEITKHLNEHGKEPSDAYLAEVLGENIEKIKEAKTASDI
YALVPIDEQFNAIEQDEITKKIEAEELLEHVQKALNQMSEREQILIQLYYFEELNLSEIKEILGITESRISQIIKEVIKK
VRKSLGVDHG
>Mature_249_residues
PKEIQKTQTSETSEKNIEKVLNAYDKQQHHHQDALAIQYLPAVRAMAFRLKERLPSSIDFNDLVSIGTEELIKLARRYES
ALNDSFWGYAKTRVNGAMLDYLRSLDVISRSNRKLIKSIDIEITKHLNEHGKEPSDAYLAEVLGENIEKIKEAKTASDIY
ALVPIDEQFNAIEQDEITKKIEAEELLEHVQKALNQMSEREQILIQLYYFEELNLSEIKEILGITESRISQIIKEVIKKV
RKSLGVDHG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for class 3 flagellar operons [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=223, Percent_Identity=34.0807174887892, Blast_Score=116, Evalue=1e-27,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 28793; Mature: 28662

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKEIQKTQTSETSEKNIEKVLNAYDKQQHHHQDALAIQYLPAVRAMAFRLKERLPSSID
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FNDLVSIGTEELIKLARRYESALNDSFWGYAKTRVNGAMLDYLRSLDVISRSNRKLIKSI
HHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIEITKHLNEHGKEPSDAYLAEVLGENIEKIKEAKTASDIYALVPIDEQFNAIEQDEITK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHCCCCHHHHHH
KIEAEELLEHVQKALNQMSEREQILIQLYYFEELNLSEIKEILGITESRISQIIKEVIKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHH
VRKSLGVDHG
HHHHCCCCCC
>Mature Secondary Structure 
PKEIQKTQTSETSEKNIEKVLNAYDKQQHHHQDALAIQYLPAVRAMAFRLKERLPSSID
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FNDLVSIGTEELIKLARRYESALNDSFWGYAKTRVNGAMLDYLRSLDVISRSNRKLIKSI
HHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIEITKHLNEHGKEPSDAYLAEVLGENIEKIKEAKTASDIYALVPIDEQFNAIEQDEITK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHCCCCHHHHHH
KIEAEELLEHVQKALNQMSEREQILIQLYYFEELNLSEIKEILGITESRISQIIKEVIKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHH
VRKSLGVDHG
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]