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The map label for this gene is lepA [H]

Identifier: 188527160

GI number: 188527160

Start: 347086

End: 348894

Strand: Direct

Name: lepA [H]

Synonym: HPSH_01835

Alternate gene names: 188527160

Gene position: 347086-348894 (Clockwise)

Preceding gene: 188527159

Following gene: 188527161

Centisome position: 21.58

GC content: 42.18

Gene sequence:

>1809_bases
ATGAAATCAAAAGTGCCAATGAAAAATATCCGCAATTTTTCCATTATCGCTCACATTGACCATGGTAAAAGCACTTTAGC
GGATTGTTTGATCGCTGAATGCAACGCTATCAGCAACAGAGAAATGACCAGCCAAGTGATGGACACTATGGATATTGAAA
AAGAAAGGGGCATTACGATTAAAGCTCAAAGCGTGCGCTTAAATTACACGCTTAAGGGAGAGGATTATGTTTTAAACCTC
ATTGACACCCCAGGGCATGTGGATTTCAGCTATGAAGTGTCGCGCTCTTTGTGTTCATGCGAAGGGGCGTTATTGGTGGT
GGATGCCACTCAAGGCGTGGAAGCGCAAACCATTGCGAATGTTTATATCGCTTTAGACAATAATTTAGAAATTTTACCGG
TGATTAATAAAATTGATTTGCCCAATGCGAATGTTTTAGAAGTCAAACAGGATATAGAAGACACGATAGGGATTGATTGC
TCTAATGCTAATGAAGTGAGCGCTAAAGCTAGGCTTGGCATTAAAGATTTGTTAGAAAAAATCATTACGACCATTCCTGC
CCCTAGCGGTGATTTTAACGCTCCCTTAAAAGCGCTCATTTATGATTCATGGTTTGACAATTATTTAGGGGCGCTAGCGT
TGGTGCGTATCATGGATGGGAGCATCAACACAGAGCAAGAAATTTTAGTGATGGGAACGGGTAAAAAACACGGCGTTTTA
GGGCTATACTACCCTAATCCTTTGAAAAAAATCCCTACTAAAAGTTTAGAATGCGGTGAGATTGGCATTGTGAGTTTAGG
GCTAAAAAGCGTTACGGATATTGCGGTAGGCGACACGCTCACAGACGCTAAAAACCCTACCCCTAAACCCATTGAAGGCT
TTATGCCGGCTAAACCCTTTGTTTTTGCGGGGCTTTACCCTATAGAAACGGACAGGTTTGAAGATTTAAGAGAAGCGTTA
TTGAAACTCCAGCTTAACGATTGCGCTTTAAATTTTGAGCCTGAAAGCTCTGTGGCGCTTGGCTTTGGCTTTAGGGTGGG
CTTTTTAGGGCTATTGCACATGGAAGTGATTAAAGAAAGGCTAGAGAGGGAATTTGGCCTTAACCTCATCGCTACCGCTC
CCACGGTGGTGTATGAAGTGCATTTGACGGATAATAGCATCAAATATGTCCAAAACCCTAGCGAACTGCCCCCTGAAAAC
CATATCGCTTGCATCAAAGAGCCTTTTGTGAGGGCGACGATCATCACGCCGAGTGAATTTTTGGGTAATTTAATGCAGTT
ATTGAACAATAAAAGAGGCATTCAAGAAAAAATGGAATATTTGAACCAATCTCGTGTCATGCTCACTTATTCCTTGCCGA
GCAACGAAATTGTGATGGATTTTTATGACAAGCTCAAATCTTGCACTAAAGGGTATGCGAGCTTTGATTATGAGCCGATA
GAAAACAGAGAGGCCCATTTAGTGAAGTTAGATGTGAGGGTGGCAGGCGATGTGGTGGATGCGCTTTCTATCATCATAGA
TAAAAACAAGGCGTATGAAAAGGGGCGAGCCTTAGTGGAAACGATGAAAGAGCTTATCCCAAGACAGCTTTTTGAAGTCG
CTATCCAAGCGAGCGTGGGGAATAAAATCATCGCCAGAGAGACGATTAAATCTGTTGGTAAGAATGTAACGGCTAAGTGC
TATGGGGGCGATATTACACGAAAAAGAAAACTTTTAGAAAAGCAAAAAGAAGGCAAGAAACGCATGAAAGCTATCGGCAA
GGTGGAGCTTCCTCAAGAAGCGTTTTTAGCGATATTAAAGATTGATTAG

Upstream 100 bases:

>100_bases
AATTTATCATGCATGGGAACACCGCTTTAGTGGAAAAATCCTTAGGTTTAGACACAGAGAGTTTGACTGACGCTATTTTA
AAAGATTTAGGACAAGAGAG

Downstream 100 bases:

>100_bases
GGTGTTTGATGAAAGTAGGCTCACTTTTTGCTGGGATTGGAGGGTTTGAATGCGCGTTTTTGCAAGCGGGCTTTGAAATT
GATTGGGCGAATGAATTGGA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MKSKVPMKNIRNFSIIAHIDHGKSTLADCLIAECNAISNREMTSQVMDTMDIEKERGITIKAQSVRLNYTLKGEDYVLNL
IDTPGHVDFSYEVSRSLCSCEGALLVVDATQGVEAQTIANVYIALDNNLEILPVINKIDLPNANVLEVKQDIEDTIGIDC
SNANEVSAKARLGIKDLLEKIITTIPAPSGDFNAPLKALIYDSWFDNYLGALALVRIMDGSINTEQEILVMGTGKKHGVL
GLYYPNPLKKIPTKSLECGEIGIVSLGLKSVTDIAVGDTLTDAKNPTPKPIEGFMPAKPFVFAGLYPIETDRFEDLREAL
LKLQLNDCALNFEPESSVALGFGFRVGFLGLLHMEVIKERLEREFGLNLIATAPTVVYEVHLTDNSIKYVQNPSELPPEN
HIACIKEPFVRATIITPSEFLGNLMQLLNNKRGIQEKMEYLNQSRVMLTYSLPSNEIVMDFYDKLKSCTKGYASFDYEPI
ENREAHLVKLDVRVAGDVVDALSIIIDKNKAYEKGRALVETMKELIPRQLFEVAIQASVGNKIIARETIKSVGKNVTAKC
YGGDITRKRKLLEKQKEGKKRMKAIGKVELPQEAFLAILKID

Sequences:

>Translated_602_residues
MKSKVPMKNIRNFSIIAHIDHGKSTLADCLIAECNAISNREMTSQVMDTMDIEKERGITIKAQSVRLNYTLKGEDYVLNL
IDTPGHVDFSYEVSRSLCSCEGALLVVDATQGVEAQTIANVYIALDNNLEILPVINKIDLPNANVLEVKQDIEDTIGIDC
SNANEVSAKARLGIKDLLEKIITTIPAPSGDFNAPLKALIYDSWFDNYLGALALVRIMDGSINTEQEILVMGTGKKHGVL
GLYYPNPLKKIPTKSLECGEIGIVSLGLKSVTDIAVGDTLTDAKNPTPKPIEGFMPAKPFVFAGLYPIETDRFEDLREAL
LKLQLNDCALNFEPESSVALGFGFRVGFLGLLHMEVIKERLEREFGLNLIATAPTVVYEVHLTDNSIKYVQNPSELPPEN
HIACIKEPFVRATIITPSEFLGNLMQLLNNKRGIQEKMEYLNQSRVMLTYSLPSNEIVMDFYDKLKSCTKGYASFDYEPI
ENREAHLVKLDVRVAGDVVDALSIIIDKNKAYEKGRALVETMKELIPRQLFEVAIQASVGNKIIARETIKSVGKNVTAKC
YGGDITRKRKLLEKQKEGKKRMKAIGKVELPQEAFLAILKID
>Mature_602_residues
MKSKVPMKNIRNFSIIAHIDHGKSTLADCLIAECNAISNREMTSQVMDTMDIEKERGITIKAQSVRLNYTLKGEDYVLNL
IDTPGHVDFSYEVSRSLCSCEGALLVVDATQGVEAQTIANVYIALDNNLEILPVINKIDLPNANVLEVKQDIEDTIGIDC
SNANEVSAKARLGIKDLLEKIITTIPAPSGDFNAPLKALIYDSWFDNYLGALALVRIMDGSINTEQEILVMGTGKKHGVL
GLYYPNPLKKIPTKSLECGEIGIVSLGLKSVTDIAVGDTLTDAKNPTPKPIEGFMPAKPFVFAGLYPIETDRFEDLREAL
LKLQLNDCALNFEPESSVALGFGFRVGFLGLLHMEVIKERLEREFGLNLIATAPTVVYEVHLTDNSIKYVQNPSELPPEN
HIACIKEPFVRATIITPSEFLGNLMQLLNNKRGIQEKMEYLNQSRVMLTYSLPSNEIVMDFYDKLKSCTKGYASFDYEPI
ENREAHLVKLDVRVAGDVVDALSIIIDKNKAYEKGRALVETMKELIPRQLFEVAIQASVGNKIIARETIKSVGKNVTAKC
YGGDITRKRKLLEKQKEGKKRMKAIGKVELPQEAFLAILKID

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=608, Percent_Identity=47.5328947368421, Blast_Score=582, Evalue=1e-166,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=49.2424242424242, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=46.3636363636364, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=46.3636363636364, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=46.3636363636364, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=42.3611111111111, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI18390331, Length=198, Percent_Identity=33.8383838383838, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI25306287, Length=142, Percent_Identity=40.8450704225352, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI19923640, Length=142, Percent_Identity=40.8450704225352, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI25306283, Length=137, Percent_Identity=41.6058394160584, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=36.0902255639098, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=36.0902255639098, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI94966752, Length=131, Percent_Identity=33.587786259542, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788922, Length=598, Percent_Identity=56.1872909698997, Blast_Score=659, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=514, Percent_Identity=28.5992217898833, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1789738, Length=208, Percent_Identity=28.8461538461538, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1790835, Length=161, Percent_Identity=32.9192546583851, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1789559, Length=220, Percent_Identity=27.2727272727273, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=613, Percent_Identity=38.8254486133768, Blast_Score=444, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI71988819, Length=136, Percent_Identity=40.4411764705882, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71988811, Length=136, Percent_Identity=40.4411764705882, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17506493, Length=157, Percent_Identity=38.2165605095541, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17556745, Length=152, Percent_Identity=36.8421052631579, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=148, Percent_Identity=35.1351351351351, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=36.8421052631579, Blast_Score=90, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=608, Percent_Identity=44.2434210526316, Blast_Score=509, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6324707, Length=169, Percent_Identity=39.0532544378698, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=169, Percent_Identity=39.0532544378698, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=180, Percent_Identity=32.2222222222222, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322359, Length=118, Percent_Identity=35.5932203389831, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324166, Length=141, Percent_Identity=38.2978723404255, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=240, Percent_Identity=25.4166666666667, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI78706572, Length=606, Percent_Identity=43.5643564356436, Blast_Score=524, Evalue=1e-149,
Organism=Drosophila melanogaster, GI24582462, Length=149, Percent_Identity=40.2684563758389, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28574573, Length=168, Percent_Identity=41.6666666666667, Blast_Score=103, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585711, Length=164, Percent_Identity=36.5853658536585, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24585713, Length=164, Percent_Identity=36.5853658536585, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24585709, Length=164, Percent_Identity=36.5853658536585, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI221458488, Length=150, Percent_Identity=37.3333333333333, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=38.3458646616541, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19921738, Length=239, Percent_Identity=28.0334728033473, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66842; Mature: 66842

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKVPMKNIRNFSIIAHIDHGKSTLADCLIAECNAISNREMTSQVMDTMDIEKERGITI
CCCCCCHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCEEE
KAQSVRLNYTLKGEDYVLNLIDTPGHVDFSYEVSRSLCSCEGALLVVDATQGVEAQTIAN
EEEEEEEEEEECCCCEEEEEECCCCCEEEEHHHHHHHHCCCCCEEEEECCCCCCCEEEEE
VYIALDNNLEILPVINKIDLPNANVLEVKQDIEDTIGIDCSNANEVSAKARLGIKDLLEK
EEEEECCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHHH
IITTIPAPSGDFNAPLKALIYDSWFDNYLGALALVRIMDGSINTEQEILVMGTGKKHGVL
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEE
GLYYPNPLKKIPTKSLECGEIGIVSLGLKSVTDIAVGDTLTDAKNPTPKPIEGFMPAKPF
EEECCCCHHHCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCE
VFAGLYPIETDRFEDLREALLKLQLNDCALNFEPESSVALGFGFRVGFLGLLHMEVIKER
EEECCCCCCCHHHHHHHHHHHHEEECCEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHH
LEREFGLNLIATAPTVVYEVHLTDNSIKYVQNPSELPPENHIACIKEPFVRATIITPSEF
HHHHHCCEEEEECCEEEEEEEECCCCEEECCCCCCCCCCCCEEEECCCCEEEEEECHHHH
LGNLMQLLNNKRGIQEKMEYLNQSRVMLTYSLPSNEIVMDFYDKLKSCTKGYASFDYEPI
HHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHCCCCCCC
ENREAHLVKLDVRVAGDVVDALSIIIDKNKAYEKGRALVETMKELIPRQLFEVAIQASVG
CCCCEEEEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NKIIARETIKSVGKNVTAKCYGGDITRKRKLLEKQKEGKKRMKAIGKVELPQEAFLAILK
CCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEE
ID
CC
>Mature Secondary Structure
MKSKVPMKNIRNFSIIAHIDHGKSTLADCLIAECNAISNREMTSQVMDTMDIEKERGITI
CCCCCCHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCEEE
KAQSVRLNYTLKGEDYVLNLIDTPGHVDFSYEVSRSLCSCEGALLVVDATQGVEAQTIAN
EEEEEEEEEEECCCCEEEEEECCCCCEEEEHHHHHHHHCCCCCEEEEECCCCCCCEEEEE
VYIALDNNLEILPVINKIDLPNANVLEVKQDIEDTIGIDCSNANEVSAKARLGIKDLLEK
EEEEECCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHHH
IITTIPAPSGDFNAPLKALIYDSWFDNYLGALALVRIMDGSINTEQEILVMGTGKKHGVL
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEE
GLYYPNPLKKIPTKSLECGEIGIVSLGLKSVTDIAVGDTLTDAKNPTPKPIEGFMPAKPF
EEECCCCHHHCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCE
VFAGLYPIETDRFEDLREALLKLQLNDCALNFEPESSVALGFGFRVGFLGLLHMEVIKER
EEECCCCCCCHHHHHHHHHHHHEEECCEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHH
LEREFGLNLIATAPTVVYEVHLTDNSIKYVQNPSELPPENHIACIKEPFVRATIITPSEF
HHHHHCCEEEEECCEEEEEEEECCCCEEECCCCCCCCCCCCEEEECCCCEEEEEECHHHH
LGNLMQLLNNKRGIQEKMEYLNQSRVMLTYSLPSNEIVMDFYDKLKSCTKGYASFDYEPI
HHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHCCCCCCC
ENREAHLVKLDVRVAGDVVDALSIIIDKNKAYEKGRALVETMKELIPRQLFEVAIQASVG
CCCCEEEEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NKIIARETIKSVGKNVTAKCYGGDITRKRKLLEKQKEGKKRMKAIGKVELPQEAFLAILK
CCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEE
ID
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA