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The map label for this gene is amiE

Identifier: 188527100

GI number: 188527100

Start: 295918

End: 296937

Strand: Direct

Name: amiE

Synonym: HPSH_01525

Alternate gene names: 188527100

Gene position: 295918-296937 (Clockwise)

Preceding gene: 188527099

Following gene: 188527102

Centisome position: 18.4

GC content: 44.8

Gene sequence:

>1020_bases
ATGAGACATGGAGATATTAGTAGCAGCCCAGATACTGTGGGTGTAGCGGTAGTTAATTATAAGATGCCTAGACTCCACAC
TAAGAATGAGGTGTTGGAAAATTGTCGCAATATCGCTAAGGTGATTGGTGGGGTCAAACAAGGTCTGCCCGGGTTGGATC
TGATTATTTTCCCTGAATACAGCACGCATGGGATTATGTATGACAGACAAGAAATGTTTGACACAGCCGCAAGCGTTCCT
GGAGAAGAAACCGCAATTCTTGCTGAGGCTTGTAAGAAAAACAAGGTTTGGGGAGTGTTCTCTTTGACGGGGGAAAAACA
CGAGCAAGCTAAAAAGAATCCCTATAATACTTTGATTCTTGTCAATGATAAGGGTGAGATCGTGCAAAAATACCGCAAAA
TCTTGCCTTGGTGCCCTATTGAATGCTGGTATCCTGGGGATAAAACTTATGTGGTTGATGGGCCTAAGGGCTTGAAAGTT
TCTTTGATTATTTGCGATGATGGTAACTACCCTGAAATTTGGCGCGATTGTGCGATGCGTGGGGCAGAACTCATTGTGCG
CTGTCAAGGTTACATGTATCCGGCTAAAGAGCAACAAATTGCGATCGTGAAAGCTATGGCGTGGGCTAATCAATGTTATG
TAGCGGTAGCGAATGCGACCGGCTTTGATGGGGTGTATTCCTATTTTGGGCATTCTAGCATTATTGGTTTTGACGGGCAT
ACTTTGGGCGAATGCGGGGAAGAAGAAAATGGTCTTCAATACGCTCAACTTTCTGTGCAACAAATCCGTGATGCGAGGAA
ATACGACCAAAGCCAAAACCAACTCTTCAAACTTTTACACAGAGGTTATAGTGGGGTTTTTGCTAGTGGCGATGGGGATA
AGGGTGTGGCGGAATGCCCTTTTGAGTTCTATAAAACTTGGGTGAATGACCCCAAAAAAGCTCAAGAAAATGTAGAAAAA
TTCACTCGCCCAAGCGTGGGTGTGGCCGCTTGTCCTGTAGGCGATTTGCCCACGAAATAA

Upstream 100 bases:

>100_bases
TTAAAGTTTTCATATTCATTACAGATCCATTTACACAACTATTTTATAAATTCAAATAGAGGGTTTGTAGGAACTCTCAT
TAAAGAACAAGGAGTATAAC

Downstream 100 bases:

>100_bases
GGGGCAAAAAGGGGGGGGGCTTTAGAGAAGCTTAAAAGCGTTTTAATATCTCTTTTTAGAACGCTTAAAAACCACCAAAA
GACTACAAATATTTCGTTAA

Product: acylamide amidohydrolase

Products: NA

Alternate protein names: Acylamide amidohydrolase [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MRHGDISSSPDTVGVAVVNYKMPRLHTKNEVLENCRNIAKVIGGVKQGLPGLDLIIFPEYSTHGIMYDRQEMFDTAASVP
GEETAILAEACKKNKVWGVFSLTGEKHEQAKKNPYNTLILVNDKGEIVQKYRKILPWCPIECWYPGDKTYVVDGPKGLKV
SLIICDDGNYPEIWRDCAMRGAELIVRCQGYMYPAKEQQIAIVKAMAWANQCYVAVANATGFDGVYSYFGHSSIIGFDGH
TLGECGEEENGLQYAQLSVQQIRDARKYDQSQNQLFKLLHRGYSGVFASGDGDKGVAECPFEFYKTWVNDPKKAQENVEK
FTRPSVGVAACPVGDLPTK

Sequences:

>Translated_339_residues
MRHGDISSSPDTVGVAVVNYKMPRLHTKNEVLENCRNIAKVIGGVKQGLPGLDLIIFPEYSTHGIMYDRQEMFDTAASVP
GEETAILAEACKKNKVWGVFSLTGEKHEQAKKNPYNTLILVNDKGEIVQKYRKILPWCPIECWYPGDKTYVVDGPKGLKV
SLIICDDGNYPEIWRDCAMRGAELIVRCQGYMYPAKEQQIAIVKAMAWANQCYVAVANATGFDGVYSYFGHSSIIGFDGH
TLGECGEEENGLQYAQLSVQQIRDARKYDQSQNQLFKLLHRGYSGVFASGDGDKGVAECPFEFYKTWVNDPKKAQENVEK
FTRPSVGVAACPVGDLPTK
>Mature_339_residues
MRHGDISSSPDTVGVAVVNYKMPRLHTKNEVLENCRNIAKVIGGVKQGLPGLDLIIFPEYSTHGIMYDRQEMFDTAASVP
GEETAILAEACKKNKVWGVFSLTGEKHEQAKKNPYNTLILVNDKGEIVQKYRKILPWCPIECWYPGDKTYVVDGPKGLKV
SLIICDDGNYPEIWRDCAMRGAELIVRCQGYMYPAKEQQIAIVKAMAWANQCYVAVANATGFDGVYSYFGHSSIIGFDGH
TLGECGEEENGLQYAQLSVQQIRDARKYDQSQNQLFKLLHRGYSGVFASGDGDKGVAECPFEFYKTWVNDPKKAQENVEK
FTRPSVGVAACPVGDLPTK

Specific function: Also exhibits in vitro acyl transferase activity, transferring the acyl moiety of short-chain amides to hydroxylamine to form hydroxamates [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 37713; Mature: 37713

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHGDISSSPDTVGVAVVNYKMPRLHTKNEVLENCRNIAKVIGGVKQGLPGLDLIIFPEY
CCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
STHGIMYDRQEMFDTAASVPGEETAILAEACKKNKVWGVFSLTGEKHEQAKKNPYNTLIL
CCCCEEECHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCEEEE
VNDKGEIVQKYRKILPWCPIECWYPGDKTYVVDGPKGLKVSLIICDDGNYPEIWRDCAMR
ECCCHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHC
GAELIVRCQGYMYPAKEQQIAIVKAMAWANQCYVAVANATGFDGVYSYFGHSSIIGFDGH
CHHEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCEEEECCCC
TLGECGEEENGLQYAQLSVQQIRDARKYDQSQNQLFKLLHRGYSGVFASGDGDKGVAECP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEECCCCCCCHHHCC
FEFYKTWVNDPKKAQENVEKFTRPSVGVAACPVGDLPTK
HHHHHHHCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MRHGDISSSPDTVGVAVVNYKMPRLHTKNEVLENCRNIAKVIGGVKQGLPGLDLIIFPEY
CCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
STHGIMYDRQEMFDTAASVPGEETAILAEACKKNKVWGVFSLTGEKHEQAKKNPYNTLIL
CCCCEEECHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCEEEE
VNDKGEIVQKYRKILPWCPIECWYPGDKTYVVDGPKGLKVSLIICDDGNYPEIWRDCAMR
ECCCHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHC
GAELIVRCQGYMYPAKEQQIAIVKAMAWANQCYVAVANATGFDGVYSYFGHSSIIGFDGH
CHHEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCEEEECCCC
TLGECGEEENGLQYAQLSVQQIRDARKYDQSQNQLFKLLHRGYSGVFASGDGDKGVAECP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEECCCCCCCHHHCC
FEFYKTWVNDPKKAQENVEKFTRPSVGVAACPVGDLPTK
HHHHHHHCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA