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The map label for this gene is phrA [H]

Identifier: 187927713

GI number: 187927713

Start: 658227

End: 659783

Strand: Reverse

Name: phrA [H]

Synonym: Rpic_0617

Alternate gene names: 187927713

Gene position: 659783-658227 (Counterclockwise)

Preceding gene: 187927721

Following gene: 187927706

Centisome position: 16.73

GC content: 64.48

Gene sequence:

>1557_bases
ATGCCCTCCACCGCCCCAAGCCGCCAGCCGTACAACATCGGAGCGCACTTCCAGCGAGGTCTGGTGTGGTTCCGGCGCGA
TCTGCGGCACTTCGACCACGCGGCGCTGCATTATGCCTTGCGGCACTGCCGCGAGGTCTATTGCGTGTTCGTGTTCGACC
GCGACATCCTCGACGCGCTGCTCGCGCGCGGGCTCCAGGCCGACCGCCGTATCGAGTTCATCCGCGCGTCCATCGAGGAA
CTGCGCAGCGCGTTACGCGAGGCCGGCGGCGACCTCATGGTGGTGCATGACCACCCTCGCCACGCGATTCCGGAGATCGC
GCGCAAGCTGAACATCGAGGCCGTCTTCGCCAACCATGACGAAGAACCTTCCGCGCAGGCGCGCGATGAAGCGGTGCGCA
AAGTGCTCGCCCAGCAGCCGTGCGCCTGGTTCGACTTCAAGGATCAGGTGATCTTCGAGCGCGACGAAATCCTGAACGGG
CAAGGCAGACCGTACGGCGTCTTCACGCCTTACAAGAATGCCTGGCTGGCCGCGCTGACGCCGTTCGACTTGCGCGCGTA
TCCGACAGAGCCCTACTTGGGCGCGCTGGCGAGGCCGCCTGAGGCACTTGCGCACCCAACGCCTGCGCTGGAAGCGATGG
GCTTCGCGCACACCAATCTGTCGGAGATTGCCCTACCCACCGGCATGTCGGGGGGCCAGGCATTGCTCGACGAGTTTGAA
GACCGCATGGACGACTACCATGCGCGCCGCGATTTTCCCGCCGTGCGCGGACCCAGCTATCTGTCCACGCACCTGCGCTT
TGGCACCGTATCGATCCGCACGCTCGCTGCGCGCGCACATGCCGCCATGCTGCGCGGCAGCCGGGGCGCGGCGACGTGGC
TGTCGGAACTGGTCTGGCGTGACTTCTATTTCATGATCCTGCATCACCGCCCGGATCTGGCCGACGGCGCCGCTTTCCAT
CCGGAATTCGATCGCATCCGCTGGGTGGACGGCGACACCGGCGACGCGCGCTTTGAAGCGTGGAAAGGTGCACAGACTGG
TTATCCGCTGGTGGATGCGGCCATGCTGCAGATCTTCCAGAGCGGCTACATGCACAACCGCCTGCGCATGGTGGCGGCCA
GTTTTCTCATCAAGGACCTTGGCATCGATTGGCGGCGCGGCGAGCAGTATTTTGCCGACGTGCTGAACGATTTCGACTTC
TCAGCCAACAACGGTGGCTGGCAGTGGGCGGCGTCCAGTGGGTGCGATGCGCAACCGTGGTTCCGCATCTTCAACCCCGT
CACGCAGTCGGAGAAGTTCGACCCGCAGGGCCGTTTCATCCGCAAGTACCTGCCGCAGTTGGCAAAGCTGCCCGACAAAT
ACATCCACGCACCGTGGACGGCACCAGCCAATGTGCTGAAGGAAGCGGGCGTCGTGCTGGGCGAGACGTATCCCCGCCCC
ATCATCGACCACGCAGGCGCACGTGCGGCCACGCTGGACCGCTATGCCGTGACCAAGGCACATCGCACATCGGGCGCTGA
AGCGGCGGCAATGAGCAAATCCAGCAACGACGATTGA

Upstream 100 bases:

>100_bases
TAGACGAGAAGAATCAGCCATGACAACAACGTGAACAAGACAAAAAGGGGCGAATTTTGCGATACGCTCGGCCAGTCTAA
AGAGCAACCGATTCCATCCG

Downstream 100 bases:

>100_bases
AGATCGCCACGCAGCAAACAACAAGGGGCCGGTAGAACACCGGCCCCTTGTTGTTTTTGTGAAGCGCGTCGTCAGCCTGC
CCTGGCCGCCGGCGCGCGCT

Product: Deoxyribodipyrimidine photo-lyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MPSTAPSRQPYNIGAHFQRGLVWFRRDLRHFDHAALHYALRHCREVYCVFVFDRDILDALLARGLQADRRIEFIRASIEE
LRSALREAGGDLMVVHDHPRHAIPEIARKLNIEAVFANHDEEPSAQARDEAVRKVLAQQPCAWFDFKDQVIFERDEILNG
QGRPYGVFTPYKNAWLAALTPFDLRAYPTEPYLGALARPPEALAHPTPALEAMGFAHTNLSEIALPTGMSGGQALLDEFE
DRMDDYHARRDFPAVRGPSYLSTHLRFGTVSIRTLAARAHAAMLRGSRGAATWLSELVWRDFYFMILHHRPDLADGAAFH
PEFDRIRWVDGDTGDARFEAWKGAQTGYPLVDAAMLQIFQSGYMHNRLRMVAASFLIKDLGIDWRRGEQYFADVLNDFDF
SANNGGWQWAASSGCDAQPWFRIFNPVTQSEKFDPQGRFIRKYLPQLAKLPDKYIHAPWTAPANVLKEAGVVLGETYPRP
IIDHAGARAATLDRYAVTKAHRTSGAEAAAMSKSSNDD

Sequences:

>Translated_518_residues
MPSTAPSRQPYNIGAHFQRGLVWFRRDLRHFDHAALHYALRHCREVYCVFVFDRDILDALLARGLQADRRIEFIRASIEE
LRSALREAGGDLMVVHDHPRHAIPEIARKLNIEAVFANHDEEPSAQARDEAVRKVLAQQPCAWFDFKDQVIFERDEILNG
QGRPYGVFTPYKNAWLAALTPFDLRAYPTEPYLGALARPPEALAHPTPALEAMGFAHTNLSEIALPTGMSGGQALLDEFE
DRMDDYHARRDFPAVRGPSYLSTHLRFGTVSIRTLAARAHAAMLRGSRGAATWLSELVWRDFYFMILHHRPDLADGAAFH
PEFDRIRWVDGDTGDARFEAWKGAQTGYPLVDAAMLQIFQSGYMHNRLRMVAASFLIKDLGIDWRRGEQYFADVLNDFDF
SANNGGWQWAASSGCDAQPWFRIFNPVTQSEKFDPQGRFIRKYLPQLAKLPDKYIHAPWTAPANVLKEAGVVLGETYPRP
IIDHAGARAATLDRYAVTKAHRTSGAEAAAMSKSSNDD
>Mature_517_residues
PSTAPSRQPYNIGAHFQRGLVWFRRDLRHFDHAALHYALRHCREVYCVFVFDRDILDALLARGLQADRRIEFIRASIEEL
RSALREAGGDLMVVHDHPRHAIPEIARKLNIEAVFANHDEEPSAQARDEAVRKVLAQQPCAWFDFKDQVIFERDEILNGQ
GRPYGVFTPYKNAWLAALTPFDLRAYPTEPYLGALARPPEALAHPTPALEAMGFAHTNLSEIALPTGMSGGQALLDEFED
RMDDYHARRDFPAVRGPSYLSTHLRFGTVSIRTLAARAHAAMLRGSRGAATWLSELVWRDFYFMILHHRPDLADGAAFHP
EFDRIRWVDGDTGDARFEAWKGAQTGYPLVDAAMLQIFQSGYMHNRLRMVAASFLIKDLGIDWRRGEQYFADVLNDFDFS
ANNGGWQWAASSGCDAQPWFRIFNPVTQSEKFDPQGRFIRKYLPQLAKLPDKYIHAPWTAPANVLKEAGVVLGETYPRPI
IDHAGARAATLDRYAVTKAHRTSGAEAAAMSKSSNDD

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=486, Percent_Identity=29.4238683127572, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI4758072, Length=516, Percent_Identity=27.5193798449612, Blast_Score=199, Evalue=6e-51,
Organism=Homo sapiens, GI188536103, Length=436, Percent_Identity=28.8990825688073, Blast_Score=186, Evalue=5e-47,
Organism=Escherichia coli, GI1786926, Length=487, Percent_Identity=38.6036960985626, Blast_Score=300, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6324962, Length=513, Percent_Identity=26.1208576998051, Blast_Score=176, Evalue=8e-45,
Organism=Drosophila melanogaster, GI17137248, Length=519, Percent_Identity=27.7456647398844, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24585455, Length=519, Percent_Identity=27.7456647398844, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24648152, Length=557, Percent_Identity=24.4165170556553, Blast_Score=148, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 58530; Mature: 58398

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTAPSRQPYNIGAHFQRGLVWFRRDLRHFDHAALHYALRHCREVYCVFVFDRDILDAL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHH
LARGLQADRRIEFIRASIEELRSALREAGGDLMVVHDHPRHAIPEIARKLNIEAVFANHD
HHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCEEEECCCC
EEPSAQARDEAVRKVLAQQPCAWFDFKDQVIFERDEILNGQGRPYGVFTPYKNAWLAALT
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHCCHHHHHCCCCCCEEECCCCCCCEEEEEC
PFDLRAYPTEPYLGALARPPEALAHPTPALEAMGFAHTNLSEIALPTGMSGGQALLDEFE
CCCCCCCCCCCHHHHHCCCCHHHCCCCHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHH
DRMDDYHARRDFPAVRGPSYLSTHLRFGTVSIRTLAARAHAAMLRGSRGAATWLSELVWR
HHHHHHHHHCCCCCCCCCHHHHHHHEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
DFYFMILHHRPDLADGAAFHPEFDRIRWVDGDTGDARFEAWKGAQTGYPLVDAAMLQIFQ
HHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHH
SGYMHNRLRMVAASFLIKDLGIDWRRGEQYFADVLNDFDFSANNGGWQWAASSGCDAQPW
HHHHHHHHHHHHHHHHHHHHCCCHHCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHH
FRIFNPVTQSEKFDPQGRFIRKYLPQLAKLPDKYIHAPWTAPANVLKEAGVVLGETYPRP
HHHHCCHHCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHCCHHCCCCCCCH
IIDHAGARAATLDRYAVTKAHRTSGAEAAAMSKSSNDD
HHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
PSTAPSRQPYNIGAHFQRGLVWFRRDLRHFDHAALHYALRHCREVYCVFVFDRDILDAL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHH
LARGLQADRRIEFIRASIEELRSALREAGGDLMVVHDHPRHAIPEIARKLNIEAVFANHD
HHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCEEEECCCC
EEPSAQARDEAVRKVLAQQPCAWFDFKDQVIFERDEILNGQGRPYGVFTPYKNAWLAALT
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHCCHHHHHCCCCCCEEECCCCCCCEEEEEC
PFDLRAYPTEPYLGALARPPEALAHPTPALEAMGFAHTNLSEIALPTGMSGGQALLDEFE
CCCCCCCCCCCHHHHHCCCCHHHCCCCHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHH
DRMDDYHARRDFPAVRGPSYLSTHLRFGTVSIRTLAARAHAAMLRGSRGAATWLSELVWR
HHHHHHHHHCCCCCCCCCHHHHHHHEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
DFYFMILHHRPDLADGAAFHPEFDRIRWVDGDTGDARFEAWKGAQTGYPLVDAAMLQIFQ
HHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHH
SGYMHNRLRMVAASFLIKDLGIDWRRGEQYFADVLNDFDFSANNGGWQWAASSGCDAQPW
HHHHHHHHHHHHHHHHHHHHCCCHHCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHH
FRIFNPVTQSEKFDPQGRFIRKYLPQLAKLPDKYIHAPWTAPANVLKEAGVVLGETYPRP
HHHHCCHHCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHCCHHCCCCCCCH
IIDHAGARAATLDRYAVTKAHRTSGAEAAAMSKSSNDD
HHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]