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The map label for this gene is purH [H]

Identifier: 187927483

GI number: 187927483

Start: 402754

End: 404328

Strand: Reverse

Name: purH [H]

Synonym: Rpic_0380

Alternate gene names: 187927483

Gene position: 404328-402754 (Counterclockwise)

Preceding gene: 187927484

Following gene: 187927482

Centisome position: 10.26

GC content: 64.83

Gene sequence:

>1575_bases
ATGATCCAGCAAGCCCTGCTTTCCGTTTCCGACAAGACCGGCATCGTCGACTTTGCCCGTGCCCTGCACGAGCGCGGCGT
CAAGCTCCTCTCCACCGGCGGCACCGCCAAGCTGCTCGCCGAGTCCAGCCTGCCCGTGACGGAAGTGGCGGACTACACCG
GCTTTCCGGAAATGCTCGACGGCCGCGTCAAGACGCTGCACCCGAAGGTGCACGGCGGCATCCTGGCCCGTCGCGACCTG
CCCGAGCACATGGCCGCACTGTCCGAACACAGCATCCCGACCATCGACCTTCTGGTGGTGAACCTGTACCCGTTCCAGCA
GACGGTGGCCAAGGACGAATGCTCGCTGGCCGACGCCATCGAGAACATCGACATCGGCGGCCCGACCATGCTGCGCTCGG
CGGCCAAGAACCACCGCGATGTGACAGTCATCGTTGACCCGGCCGACTACGCCACCGTGCTGGCCGAAATGCAGGCCAAC
AACAACACGGTCGGCTACGAAACCAACTTCATGCTGGCCAAGAAGGTGTTCGCGCACACCGCGCAGTACGACGGCGCCAT
CACCAACTACCTGACGAGCCTCGGCGCCGACAAGTCGCACAGCACGCGCAGCGCCTATCCGCAGACGCTGAACCTCGCCT
TCGACAAGGTGCAGGAGATGCGTTACGGCGAAAACCCGCATCAGTCCGCCGCGTTCTACCGCGACCTGAAAGCCGTCGAC
GGCGCGCTCGCCAATTACAAGCAACTGCAGGGCAAGGAGCTGTCGTACAACAACATCGCCGACGCCGATGCCGCGTGGGA
ATGCGTGAAGTCGTTCGATGCGGCCCACGGCGCTGCATGCGTCATCATCAAGCACGCCAACCCGTGCGGCGTGGCCATCG
GCGGCACCGCGCAGGAAGCGTATGAGAAGGCCTTCAAGACCGACTCGACCTCGGCCTTCGGCGGCATCATCGCCTTCAAC
GTGCCGCTGGATGAAGCAGCCGCACAGGTGGTGGCCAAGCAGTTCGTGGAAGTGCTGATCGCCCCGGGCTTCTCGGAAGG
CGCACGTGCCGTGTTCGCCGCCAAGCAAAACGTGCGTGTGCTGGAGATTCCGTTGGGCAAGGGCGTGAATGCGTACGACT
TCAAGCGCGTTGGCGGCGGCCTGCTGGTGCAGAGCCCGGACGCCAAGAACGTGCAGCCGAGCGAGCTGCGCGTGGTTACC
AAGCGCCACCCCACGCCAAAGGAAATGGACGACCTGATGTTTGCCTGGCGCGTCGCCAAGTTCGTCAAGTCCAACGCCAT
CGTGTTCTGCGGCGGCGGCATGACGCTGGGTGTGGGCGCCGGCCAGATGAGCCGTGTGGACTCGGCCCGCATCGCCAGCA
TCAAGGCACAGAACGCAGGCCTGACGCTGGCCGGTTCGGCTGTGGCATCGGATGCGTTCTTCCCGTTTCGCGACGGCCTG
GACGTGGTGGTCGATGCTGGCGCCTCGTGCGTGATCCAGCCGGGCGGCTCGGTACGCGACGATGAAGTGATCGCCGCCGC
CGACGAACGCAACGTGGCGATGATCTTCACGGGCACGCGCCACTTCCGCCACTAA

Upstream 100 bases:

>100_bases
CTGAGGTTGCGGGCCGGCCTTTGAACCGATTTCCTTTCTGACGGCGCCTCCTGCCAGTTCGGCAAACCCAGCGCCACACC
CGCCCACCGGCGTTTCCACC

Downstream 100 bases:

>100_bases
GCAACATCGTCCACGCGACGAACGAACGGGGCAAGGCGTAAACTGGCGCCTTGCCCCGTTTCCATTTCTGCCCACTTCCT
TCATGCGCATCCTCGGCATC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MIQQALLSVSDKTGIVDFARALHERGVKLLSTGGTAKLLAESSLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMAALSEHSIPTIDLLVVNLYPFQQTVAKDECSLADAIENIDIGGPTMLRSAAKNHRDVTVIVDPADYATVLAEMQAN
NNTVGYETNFMLAKKVFAHTAQYDGAITNYLTSLGADKSHSTRSAYPQTLNLAFDKVQEMRYGENPHQSAAFYRDLKAVD
GALANYKQLQGKELSYNNIADADAAWECVKSFDAAHGAACVIIKHANPCGVAIGGTAQEAYEKAFKTDSTSAFGGIIAFN
VPLDEAAAQVVAKQFVEVLIAPGFSEGARAVFAAKQNVRVLEIPLGKGVNAYDFKRVGGGLLVQSPDAKNVQPSELRVVT
KRHPTPKEMDDLMFAWRVAKFVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGL
DVVVDAGASCVIQPGGSVRDDEVIAAADERNVAMIFTGTRHFRH

Sequences:

>Translated_524_residues
MIQQALLSVSDKTGIVDFARALHERGVKLLSTGGTAKLLAESSLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMAALSEHSIPTIDLLVVNLYPFQQTVAKDECSLADAIENIDIGGPTMLRSAAKNHRDVTVIVDPADYATVLAEMQAN
NNTVGYETNFMLAKKVFAHTAQYDGAITNYLTSLGADKSHSTRSAYPQTLNLAFDKVQEMRYGENPHQSAAFYRDLKAVD
GALANYKQLQGKELSYNNIADADAAWECVKSFDAAHGAACVIIKHANPCGVAIGGTAQEAYEKAFKTDSTSAFGGIIAFN
VPLDEAAAQVVAKQFVEVLIAPGFSEGARAVFAAKQNVRVLEIPLGKGVNAYDFKRVGGGLLVQSPDAKNVQPSELRVVT
KRHPTPKEMDDLMFAWRVAKFVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGL
DVVVDAGASCVIQPGGSVRDDEVIAAADERNVAMIFTGTRHFRH
>Mature_524_residues
MIQQALLSVSDKTGIVDFARALHERGVKLLSTGGTAKLLAESSLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMAALSEHSIPTIDLLVVNLYPFQQTVAKDECSLADAIENIDIGGPTMLRSAAKNHRDVTVIVDPADYATVLAEMQAN
NNTVGYETNFMLAKKVFAHTAQYDGAITNYLTSLGADKSHSTRSAYPQTLNLAFDKVQEMRYGENPHQSAAFYRDLKAVD
GALANYKQLQGKELSYNNIADADAAWECVKSFDAAHGAACVIIKHANPCGVAIGGTAQEAYEKAFKTDSTSAFGGIIAFN
VPLDEAAAQVVAKQFVEVLIAPGFSEGARAVFAAKQNVRVLEIPLGKGVNAYDFKRVGGGLLVQSPDAKNVQPSELRVVT
KRHPTPKEMDDLMFAWRVAKFVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGL
DVVVDAGASCVIQPGGSVRDDEVIAAADERNVAMIFTGTRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=486, Percent_Identity=36.6255144032922, Blast_Score=269, Evalue=6e-72,
Organism=Escherichia coli, GI1790439, Length=530, Percent_Identity=61.5094339622641, Blast_Score=641, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=609, Percent_Identity=32.8407224958949, Blast_Score=278, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI71985574, Length=314, Percent_Identity=27.0700636942675, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71985556, Length=130, Percent_Identity=34.6153846153846, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323768, Length=480, Percent_Identity=35.4166666666667, Blast_Score=259, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6323056, Length=488, Percent_Identity=34.4262295081967, Blast_Score=253, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24649832, Length=483, Percent_Identity=36.6459627329193, Blast_Score=259, Evalue=5e-69,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56016; Mature: 56016

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQQALLSVSDKTGIVDFARALHERGVKLLSTGGTAKLLAESSLPVTEVADYTGFPEMLD
CCHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMAALSEHSIPTIDLLVVNLYPFQQTVAKDECSLADAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHHHHHHH
ENIDIGGPTMLRSAAKNHRDVTVIVDPADYATVLAEMQANNNTVGYETNFMLAKKVFAHT
HHCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHH
AQYDGAITNYLTSLGADKSHSTRSAYPQTLNLAFDKVQEMRYGENPHQSAAFYRDLKAVD
HHCCCHHHHHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GALANYKQLQGKELSYNNIADADAAWECVKSFDAAHGAACVIIKHANPCGVAIGGTAQEA
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEECCCHHHH
YEKAFKTDSTSAFGGIIAFNVPLDEAAAQVVAKQFVEVLIAPGFSEGARAVFAAKQNVRV
HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEE
LEIPLGKGVNAYDFKRVGGGLLVQSPDAKNVQPSELRVVTKRHPTPKEMDDLMFAWRVAK
EEEECCCCCCCHHHHHCCCEEEEECCCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHH
FVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGL
HHHCCEEEEECCCEEEEECCCHHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCC
DVVVDAGASCVIQPGGSVRDDEVIAAADERNVAMIFTGTRHFRH
EEEEECCCEEEECCCCCCCCCCEEEEECCCCEEEEEECCHHCCC
>Mature Secondary Structure
MIQQALLSVSDKTGIVDFARALHERGVKLLSTGGTAKLLAESSLPVTEVADYTGFPEMLD
CCHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMAALSEHSIPTIDLLVVNLYPFQQTVAKDECSLADAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHHHHHHH
ENIDIGGPTMLRSAAKNHRDVTVIVDPADYATVLAEMQANNNTVGYETNFMLAKKVFAHT
HHCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHH
AQYDGAITNYLTSLGADKSHSTRSAYPQTLNLAFDKVQEMRYGENPHQSAAFYRDLKAVD
HHCCCHHHHHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GALANYKQLQGKELSYNNIADADAAWECVKSFDAAHGAACVIIKHANPCGVAIGGTAQEA
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEECCCHHHH
YEKAFKTDSTSAFGGIIAFNVPLDEAAAQVVAKQFVEVLIAPGFSEGARAVFAAKQNVRV
HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEE
LEIPLGKGVNAYDFKRVGGGLLVQSPDAKNVQPSELRVVTKRHPTPKEMDDLMFAWRVAK
EEEECCCCCCCHHHHHCCCEEEEECCCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHH
FVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGL
HHHCCEEEEECCCEEEEECCCHHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCC
DVVVDAGASCVIQPGGSVRDDEVIAAADERNVAMIFTGTRHFRH
EEEEECCCEEEECCCCCCCCCCEEEEECCCCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]