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The map label for this gene is ruvC

Identifier: 187927482

GI number: 187927482

Start: 402126

End: 402671

Strand: Reverse

Name: ruvC

Synonym: Rpic_0379

Alternate gene names: 187927482

Gene position: 402671-402126 (Counterclockwise)

Preceding gene: 187927483

Following gene: 187927481

Centisome position: 10.21

GC content: 67.22

Gene sequence:

>546_bases
ATGCGCATCCTCGGCATCGATCCCGGCCTTCGCACCACGGGTTTCGGCGTGCTCGAACGGCACGGCCACAAGCTCGTCTA
CGTGGCCTCGGGCACGATCAAGAGCAACGGCAATGCCGACCTGCCCAGCCGCCTGAAGACGCTGTATGACGGCGTGTCCG
AACTGGTGAGCACATACCGGCCGGACTGCGCTTCCATCGAAAAGGTGTTCGTCAACGTCAACCCGCAATCGACGCTGCTG
CTGGGCCAAGCCCGCGGCGCGGTGATCTGCGGCCTGATGAGCGGCAACCTGCCCGTGTTCGAGTACACCGCGCTACAACT
GAAGCAGGCCGTGGTCGGCTATGGCCGCGCCAACAAGGATCAGGTGCAGGAGATGGTCGTGCGCCTGCTCAACCTCGAGG
GCAAACCCGGCGCGGACGCATCCGACGCCCTGGGCGTGGCGATCTGCCATGCGCACGGCGGCGAGACGCTGGCCGCCATG
GCCGGTCTGGCGCCACAGCTTGCGCAAAAGGGGCTGCGCGTGCGGCGCGGCCGCCTCGTCGGCTGA

Upstream 100 bases:

>100_bases
CGCCACTTCCGCCACTAAGCAACATCGTCCACGCGACGAACGAACGGGGCAAGGCGTAAACTGGCGCCTTGCCCCGTTTC
CATTTCTGCCCACTTCCTTC

Downstream 100 bases:

>100_bases
CAGGATTCTTGAAGCCCGCAGGTAGCGGCATCCGGGCACGGCGACTACCATGCGGGTACCCGTATTCAGGAGCCCCCGAT
GCCTCGCCGCCTCATCCTGC

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MRILGIDPGLRTTGFGVLERHGHKLVYVASGTIKSNGNADLPSRLKTLYDGVSELVSTYRPDCASIEKVFVNVNPQSTLL
LGQARGAVICGLMSGNLPVFEYTALQLKQAVVGYGRANKDQVQEMVVRLLNLEGKPGADASDALGVAICHAHGGETLAAM
AGLAPQLAQKGLRVRRGRLVG

Sequences:

>Translated_181_residues
MRILGIDPGLRTTGFGVLERHGHKLVYVASGTIKSNGNADLPSRLKTLYDGVSELVSTYRPDCASIEKVFVNVNPQSTLL
LGQARGAVICGLMSGNLPVFEYTALQLKQAVVGYGRANKDQVQEMVVRLLNLEGKPGADASDALGVAICHAHGGETLAAM
AGLAPQLAQKGLRVRRGRLVG
>Mature_181_residues
MRILGIDPGLRTTGFGVLERHGHKLVYVASGTIKSNGNADLPSRLKTLYDGVSELVSTYRPDCASIEKVFVNVNPQSTLL
LGQARGAVICGLMSGNLPVFEYTALQLKQAVVGYGRANKDQVQEMVVRLLNLEGKPGADASDALGVAICHAHGGETLAAM
AGLAPQLAQKGLRVRRGRLVG

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=177, Percent_Identity=49.1525423728814, Blast_Score=144, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_RALPJ (B2UFL4)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001897969.1
- ProteinModelPortal:   B2UFL4
- SMR:   B2UFL4
- GeneID:   6289676
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0379
- HOGENOM:   HBG437552
- OMA:   DCAAIEK
- ProtClustDB:   PRK00039
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 19134; Mature: 19134

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILGIDPGLRTTGFGVLERHGHKLVYVASGTIKSNGNADLPSRLKTLYDGVSELVSTYR
CEEEECCCCCCCCCCHHHHHCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHCC
PDCASIEKVFVNVNPQSTLLLGQARGAVICGLMSGNLPVFEYTALQLKQAVVGYGRANKD
CCHHCCEEEEEECCCCCEEEEECCCCEEEEEEECCCCCEEHHHHHHHHHHHHHCCCCCHH
QVQEMVVRLLNLEGKPGADASDALGVAICHAHGGETLAAMAGLAPQLAQKGLRVRRGRLV
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHCCCHHHHHCCHHHHCCCCC
G
C
>Mature Secondary Structure
MRILGIDPGLRTTGFGVLERHGHKLVYVASGTIKSNGNADLPSRLKTLYDGVSELVSTYR
CEEEECCCCCCCCCCHHHHHCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHCC
PDCASIEKVFVNVNPQSTLLLGQARGAVICGLMSGNLPVFEYTALQLKQAVVGYGRANKD
CCHHCCEEEEEECCCCCEEEEECCCCEEEEEEECCCCCEEHHHHHHHHHHHHHCCCCCHH
QVQEMVVRLLNLEGKPGADASDALGVAICHAHGGETLAAMAGLAPQLAQKGLRVRRGRLV
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHCCCHHHHHCCHHHHCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA