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The map label for this gene is mutM

Identifier: 187927361

GI number: 187927361

Start: 268808

End: 269683

Strand: Direct

Name: mutM

Synonym: Rpic_0254

Alternate gene names: 187927361

Gene position: 268808-269683 (Clockwise)

Preceding gene: 187927353

Following gene: 187927362

Centisome position: 6.82

GC content: 67.58

Gene sequence:

>876_bases
ATGCCTGAGTTGCCGGAGGTCGAGGTCACTCGCCTGGGCTTGCTACCGCATATTACTGGGCGACGTATCGTGCGCGCCGT
GGTGCGCCATCACGGCTTGCGCTGGCCCGTCGATCCGGCGCTGCCGGAGTTGCTCGCTGGCCTGACCGTTACGCGTCTGC
TGCGCCGGGGCAAATACCTGCTCATCGAATGCGTGCCCGAGGTGGAGCAGTCCGGCCGCGCCGCCGATACTGTGGGGGGC
TGGCTGCTCATCCATCTTGGGATGACGGGCACCTTGCGCGTGCTGGAAACGCCCGTGCCGCCAGGCCTGCACGATCACGT
CGATATCGAACTCGCCGGCGCAACTGGCGTACACGTCACACTGCGCTATCGCGACCCGCGCCGGTTTGGTGCCGTGCTTT
GGCATGCAGGCGATGAAGCCGGCTTGGCCGAACACCCGCTGCTGCGCAACCTCGGCATCGAGCCGTTCGACGCGCGATTC
GACGGCGACTGGATGTTTGCCCGCACGCGCGGGCGCCGGGTGGCCATCAAGTCGGCGCTGCTGGCCGGCGACATCGTGGT
CGGCGTGGGCAATATCTATTGTTCGGAGAGCCTGTTTCGCGCCGGTATCCGGCCGACTACGGCGGCGGGCCGCATCAGCC
GGCCACGCTATGCGGCGTTGGCCGATGCCATTCGTGCCACCTTGGCCGATGCCATCGCGCGTGGGGGCAGCACGCTGCGC
GACTTTGTCGGCTCGGACGGACAAAGCGGCTACTTCCAGCTTGAAGCCTTCGTGTACGACCGCGCCGGTCTGCCGTGCCG
CGCGTGCGGCACTCCGATCCGCCAGATCGTGCAGGGCCAGCGCTCCACGTTCTGTTGCCCGACCTGCCAGCGCTGA

Upstream 100 bases:

>100_bases
GTCTCGAAGCTCCGGATAGGGGGTCAGGGCATTGTAGCCTGACCGCTACAATCGTCCGCATCGTAAAAAAAGTTGGAGCA
GCACATGGGATGGAGGTTCG

Downstream 100 bases:

>100_bases
TCGGCCTGCGAAAGGCATCGAAAGCCGCCTGGGAACGTGGCGCTGGAGCGATCTGGCCGCCATTTCGCAAGGTTTTTGAA
GTGGGGTGTGGGGCCGCCGA

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MPELPEVEVTRLGLLPHITGRRIVRAVVRHHGLRWPVDPALPELLAGLTVTRLLRRGKYLLIECVPEVEQSGRAADTVGG
WLLIHLGMTGTLRVLETPVPPGLHDHVDIELAGATGVHVTLRYRDPRRFGAVLWHAGDEAGLAEHPLLRNLGIEPFDARF
DGDWMFARTRGRRVAIKSALLAGDIVVGVGNIYCSESLFRAGIRPTTAAGRISRPRYAALADAIRATLADAIARGGSTLR
DFVGSDGQSGYFQLEAFVYDRAGLPCRACGTPIRQIVQGQRSTFCCPTCQR

Sequences:

>Translated_291_residues
MPELPEVEVTRLGLLPHITGRRIVRAVVRHHGLRWPVDPALPELLAGLTVTRLLRRGKYLLIECVPEVEQSGRAADTVGG
WLLIHLGMTGTLRVLETPVPPGLHDHVDIELAGATGVHVTLRYRDPRRFGAVLWHAGDEAGLAEHPLLRNLGIEPFDARF
DGDWMFARTRGRRVAIKSALLAGDIVVGVGNIYCSESLFRAGIRPTTAAGRISRPRYAALADAIRATLADAIARGGSTLR
DFVGSDGQSGYFQLEAFVYDRAGLPCRACGTPIRQIVQGQRSTFCCPTCQR
>Mature_290_residues
PELPEVEVTRLGLLPHITGRRIVRAVVRHHGLRWPVDPALPELLAGLTVTRLLRRGKYLLIECVPEVEQSGRAADTVGGW
LLIHLGMTGTLRVLETPVPPGLHDHVDIELAGATGVHVTLRYRDPRRFGAVLWHAGDEAGLAEHPLLRNLGIEPFDARFD
GDWMFARTRGRRVAIKSALLAGDIVVGVGNIYCSESLFRAGIRPTTAAGRISRPRYAALADAIRATLADAIARGGSTLRD
FVGSDGQSGYFQLEAFVYDRAGLPCRACGTPIRQIVQGQRSTFCCPTCQR

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=291, Percent_Identity=46.0481099656357, Blast_Score=242, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_RALPJ (B2UES0)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001897848.1
- ProteinModelPortal:   B2UES0
- SMR:   B2UES0
- GeneID:   6286867
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0254
- HOGENOM:   HBG690070
- OMA:   RSTFYCA
- ProtClustDB:   PRK01103
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 31777; Mature: 31646

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 58-58 ACT_SITE 281-281 BINDING 104-104 BINDING 127-127 BINDING 172-172

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVEVTRLGLLPHITGRRIVRAVVRHHGLRWPVDPALPELLAGLTVTRLLRRGKYL
CCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
LIECVPEVEQSGRAADTVGGWLLIHLGMTGTLRVLETPVPPGLHDHVDIELAGATGVHVT
EEEECHHHHHCCCCHHHHCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEE
LRYRDPRRFGAVLWHAGDEAGLAEHPLLRNLGIEPFDARFDGDWMFARTRGRRVAIKSAL
EEECCCHHHCEEEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCEEEEECCCCEEEHHHHH
LAGDIVVGVGNIYCSESLFRAGIRPTTAAGRISRPRYAALADAIRATLADAIARGGSTLR
HHCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
DFVGSDGQSGYFQLEAFVYDRAGLPCRACGTPIRQIVQGQRSTFCCPTCQR
HHHCCCCCCCEEEEEEEEECCCCCCHHHCCHHHHHHHCCCCCEECCCCCCC
>Mature Secondary Structure 
PELPEVEVTRLGLLPHITGRRIVRAVVRHHGLRWPVDPALPELLAGLTVTRLLRRGKYL
CCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
LIECVPEVEQSGRAADTVGGWLLIHLGMTGTLRVLETPVPPGLHDHVDIELAGATGVHVT
EEEECHHHHHCCCCHHHHCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEE
LRYRDPRRFGAVLWHAGDEAGLAEHPLLRNLGIEPFDARFDGDWMFARTRGRRVAIKSAL
EEECCCHHHCEEEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCEEEEECCCCEEEHHHHH
LAGDIVVGVGNIYCSESLFRAGIRPTTAAGRISRPRYAALADAIRATLADAIARGGSTLR
HHCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
DFVGSDGQSGYFQLEAFVYDRAGLPCRACGTPIRQIVQGQRSTFCCPTCQR
HHHCCCCCCCEEEEEEEEECCCCCCHHHCCHHHHHHHCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA