Click here to switch to the map view.

The map label for this gene is ipk

Identifier: 187927358

GI number: 187927358

Start: 265262

End: 266131

Strand: Reverse

Name: ipk

Synonym: Rpic_0251

Alternate gene names: 187927358

Gene position: 266131-265262 (Counterclockwise)

Preceding gene: 187927359

Following gene: 187927357

Centisome position: 6.75

GC content: 61.95

Gene sequence:

>870_bases
ATGTCTTCCGCCCCCGTCGAGCTGCGTGATTGCCCCGCGCCTGCCAAGCTCAATCTCTTCCTGCATGTAGTCGGCCGCCG
GCCCGATGGCTACCACCTGCTGCAAACCGTCTTCCAGTTGATCGACTGGAGCGACACGCTGCATTTCGCGCGCCGCCCCG
ACCGCCGCCTCGTGCGCACCACCGACATCCTCGGCGTGCCCGCCGATGACGACCTTGTGATCCGCGCCGCGCGCCTGCTG
CAAGCGGAAACCGGCTGCACCTACGGCGTCGACATCGCCATCGAGAAACGCCTGCCGATGGGCGGAGGCCTTGGTGGTGG
CTCGTCTGATGCCGCCACCACGCTGCTCGCGCTCAATCGCCTTTGGGGCCTTGATCTGCCGCGCGCGCAACTGATGAAGA
TCGGCCTCAAGCTCGGCGCCGACGTGCCCTTCTTTGTATTCGGCCAGAACGCCTTCGCCGAGGGGATCGGCGAAAAACTC
ACGCCCATCACGCTGCCGCCGGCTGCGTTCGTGGTGATTCACCCACGCGTGCATGTGCCCACCCCTGCAATTTTTTCAGA
TGAAGGGTTGACACGCGATACGCCACTCACCATAATTACGGACTTTCCTGACCAACAAATTGTTTTCGCTTACGGTCGCA
ACGATCTACAAGCGGTGGCGGAAAGGAAATACGGCGAAATCGCCCGAGCACTTGTCTGGCTGCGTCAGTTCAGCCCTCTG
GCAAGAATGACCGGATCCGGCGCCTGCGTATTTGCTCCGTTCGACAACGTTGAGCATGCGCAAGCGGTGGCGGATCAGGT
GCCTTCCGAATGGGAAGGTCGGTGTGCGGCAGGTCTGACGCATCACCCGCTCGCAAGATTTGCTGTGTAA

Upstream 100 bases:

>100_bases
CCAAGGTCGGCATCCGCCGGCTCGATCTTGATCGCCCGCAGGGCACCAACGGCCCGCTCTCGGTGCGGCTCGTGCTCGAC
CAGTAACGCCTCGCTTCGCC

Downstream 100 bases:

>100_bases
AGTTTGCTGTTATCGCTTCTGCAATGGAGGCGGTAACCGATAGGTTGTGTAGGGGCGTCGCCAAGCTGGTTAAGGCACCG
GATTTTGATTCCGGCATGCG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSSAPVELRDCPAPAKLNLFLHVVGRRPDGYHLLQTVFQLIDWSDTLHFARRPDRRLVRTTDILGVPADDDLVIRAARLL
QAETGCTYGVDIAIEKRLPMGGGLGGGSSDAATTLLALNRLWGLDLPRAQLMKIGLKLGADVPFFVFGQNAFAEGIGEKL
TPITLPPAAFVVIHPRVHVPTPAIFSDEGLTRDTPLTIITDFPDQQIVFAYGRNDLQAVAERKYGEIARALVWLRQFSPL
ARMTGSGACVFAPFDNVEHAQAVADQVPSEWEGRCAAGLTHHPLARFAV

Sequences:

>Translated_289_residues
MSSAPVELRDCPAPAKLNLFLHVVGRRPDGYHLLQTVFQLIDWSDTLHFARRPDRRLVRTTDILGVPADDDLVIRAARLL
QAETGCTYGVDIAIEKRLPMGGGLGGGSSDAATTLLALNRLWGLDLPRAQLMKIGLKLGADVPFFVFGQNAFAEGIGEKL
TPITLPPAAFVVIHPRVHVPTPAIFSDEGLTRDTPLTIITDFPDQQIVFAYGRNDLQAVAERKYGEIARALVWLRQFSPL
ARMTGSGACVFAPFDNVEHAQAVADQVPSEWEGRCAAGLTHHPLARFAV
>Mature_288_residues
SSAPVELRDCPAPAKLNLFLHVVGRRPDGYHLLQTVFQLIDWSDTLHFARRPDRRLVRTTDILGVPADDDLVIRAARLLQ
AETGCTYGVDIAIEKRLPMGGGLGGGSSDAATTLLALNRLWGLDLPRAQLMKIGLKLGADVPFFVFGQNAFAEGIGEKLT
PITLPPAAFVVIHPRVHVPTPAIFSDEGLTRDTPLTIITDFPDQQIVFAYGRNDLQAVAERKYGEIARALVWLRQFSPLA
RMTGSGACVFAPFDNVEHAQAVADQVPSEWEGRCAAGLTHHPLARFAV

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=281, Percent_Identity=46.9750889679715, Blast_Score=227, Evalue=8e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_RALPJ (B2UER7)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001897845.1
- ProteinModelPortal:   B2UER7
- SMR:   B2UER7
- GeneID:   6289087
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0251
- HOGENOM:   HBG734593
- OMA:   LNLFLHV
- ProtClustDB:   PRK00343
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31506; Mature: 31374

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: ACT_SITE 16-16 ACT_SITE 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAPVELRDCPAPAKLNLFLHVVGRRPDGYHLLQTVFQLIDWSDTLHFARRPDRRLVRT
CCCCCCCHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCEEEH
TDILGVPADDDLVIRAARLLQAETGCTYGVDIAIEKRLPMGGGLGGGSSDAATTLLALNR
HHEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
LWGLDLPRAQLMKIGLKLGADVPFFVFGQNAFAEGIGEKLTPITLPPAAFVVIHPRVHVP
HHCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCCEEECCCEEEEECCEECCC
TPAIFSDEGLTRDTPLTIITDFPDQQIVFAYGRNDLQAVAERKYGEIARALVWLRQFSPL
CCCEECCCCCCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHH
ARMTGSGACVFAPFDNVEHAQAVADQVPSEWEGRCAAGLTHHPLARFAV
HHHCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
SSAPVELRDCPAPAKLNLFLHVVGRRPDGYHLLQTVFQLIDWSDTLHFARRPDRRLVRT
CCCCCCHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCEEEH
TDILGVPADDDLVIRAARLLQAETGCTYGVDIAIEKRLPMGGGLGGGSSDAATTLLALNR
HHEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
LWGLDLPRAQLMKIGLKLGADVPFFVFGQNAFAEGIGEKLTPITLPPAAFVVIHPRVHVP
HHCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCCEEECCCEEEEECCEECCC
TPAIFSDEGLTRDTPLTIITDFPDQQIVFAYGRNDLQAVAERKYGEIARALVWLRQFSPL
CCCEECCCCCCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHH
ARMTGSGACVFAPFDNVEHAQAVADQVPSEWEGRCAAGLTHHPLARFAV
HHHCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA