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The map label for this gene is prs [H]

Identifier: 187927357

GI number: 187927357

Start: 264104

End: 265054

Strand: Reverse

Name: prs [H]

Synonym: Rpic_0250

Alternate gene names: 187927357

Gene position: 265054-264104 (Counterclockwise)

Preceding gene: 187927358

Following gene: 187927356

Centisome position: 6.72

GC content: 61.62

Gene sequence:

>951_bases
ATGAGCAGCGAAGGCTTGATGGTTTTTACCGGCAACGCCAACCCGAAACTCGCCGAAGCTGTCGTCAAGCACCTAGGCAT
TCCGCTAGGCAAGGCCCTCGTCGGCCGATTCTCCGATGGTGAAGTCCAGGTCGAGATCCAGGAAAACGTGCGCGGCAAGC
ACGTATTCATCCTGCAATCCACCTGCGCCCCGACCAACGACAACCTGATGGAATTGATGGTGATGGTCGATGCGCTCAAG
CGTGCGTCTGCGCGCCGCATCACGGCCGCCATCCCCTACTTCGGCTATGCCCGCCAGGACCGTCGCCCGCGTTCGGCGCG
TGTGGCGATCACGGCCAAGGTCGTGGCCAACATGCTAGAGGTCGCCGGCGTCGAACGCGTGCTGACGATGGACCTCCACG
CCGACCAGATCCAAGGCTTCTTCGATATCCCGGTCGACAACATCTACGCGTCGCCGATCCTGCTCGAAGACCTGCGCAAG
AAGAACTACGAAGACCTGCTGGTGGTATCACCCGACGTCGGCGGTGTCGTGCGTGCACGTGCACTGGCCAAGCAACTCGG
CGTGGATCTGGCGATCATCGACAAACGTCGCCCGAAAGCCAACGTCGCCGAGGTGATGAACATCATCGGTGAAGTCGACG
GCCGCAACTGCGTGATCATGGACGACATGATCGACACCGGCGGCACGCTGTGCAAAGCCGCGCAGGTACTCAAGGAACGC
GGTGCCAAGCAAGTGTTCTCGTATTGCACGCACCCGGTGCTGTCGGGTGGTGCGGCCGCCCGCATTGCAGACTCGGCGCT
CGACGAAGTGGTCGTGACCGATACCATCCCGCTGCGCGAAGACGCCATCAAGTGCGGCAAGATCCGCCAACTCTCGACCG
CGCCGCTGCTGGCCGAGACCTTCACCCGCATCGTGCGCGGTGATTCGATCATGTCGCTGTTTGCTGAATAA

Upstream 100 bases:

>100_bases
GAATCCTTCCGCCCCTGCCATTTTCTTCCTCATATTTCCAGCAATAGCCCGGTTGGTGTTAACTGACCGGCGCGTCTGAA
GTCGAAAAACAGGTGCCCCG

Downstream 100 bases:

>100_bases
TTCGGCACCCGAGCGGCTTACTGTTATAATTCAAGGCTTTACTGCAGAAAACGTCGGTTAGACGGCTCTTCTGCGGTGTC
TGCGGGGCGCAATATGGCGC

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSSEGLMVFTGNANPKLAEAVVKHLGIPLGKALVGRFSDGEVQVEIQENVRGKHVFILQSTCAPTNDNLMELMVMVDALK
RASARRITAAIPYFGYARQDRRPRSARVAITAKVVANMLEVAGVERVLTMDLHADQIQGFFDIPVDNIYASPILLEDLRK
KNYEDLLVVSPDVGGVVRARALAKQLGVDLAIIDKRRPKANVAEVMNIIGEVDGRNCVIMDDMIDTGGTLCKAAQVLKER
GAKQVFSYCTHPVLSGGAAARIADSALDEVVVTDTIPLREDAIKCGKIRQLSTAPLLAETFTRIVRGDSIMSLFAE

Sequences:

>Translated_316_residues
MSSEGLMVFTGNANPKLAEAVVKHLGIPLGKALVGRFSDGEVQVEIQENVRGKHVFILQSTCAPTNDNLMELMVMVDALK
RASARRITAAIPYFGYARQDRRPRSARVAITAKVVANMLEVAGVERVLTMDLHADQIQGFFDIPVDNIYASPILLEDLRK
KNYEDLLVVSPDVGGVVRARALAKQLGVDLAIIDKRRPKANVAEVMNIIGEVDGRNCVIMDDMIDTGGTLCKAAQVLKER
GAKQVFSYCTHPVLSGGAAARIADSALDEVVVTDTIPLREDAIKCGKIRQLSTAPLLAETFTRIVRGDSIMSLFAE
>Mature_315_residues
SSEGLMVFTGNANPKLAEAVVKHLGIPLGKALVGRFSDGEVQVEIQENVRGKHVFILQSTCAPTNDNLMELMVMVDALKR
ASARRITAAIPYFGYARQDRRPRSARVAITAKVVANMLEVAGVERVLTMDLHADQIQGFFDIPVDNIYASPILLEDLRKK
NYEDLLVVSPDVGGVVRARALAKQLGVDLAIIDKRRPKANVAEVMNIIGEVDGRNCVIMDDMIDTGGTLCKAAQVLKERG
AKQVFSYCTHPVLSGGAAARIADSALDEVVVTDTIPLREDAIKCGKIRQLSTAPLLAETFTRIVRGDSIMSLFAE

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=314, Percent_Identity=45.859872611465, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI84875539, Length=316, Percent_Identity=45.253164556962, Blast_Score=291, Evalue=6e-79,
Organism=Homo sapiens, GI4506127, Length=314, Percent_Identity=45.859872611465, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI28557709, Length=314, Percent_Identity=45.5414012738853, Blast_Score=289, Evalue=2e-78,
Organism=Homo sapiens, GI4506133, Length=346, Percent_Identity=36.4161849710983, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI194018537, Length=345, Percent_Identity=37.1014492753623, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI310128524, Length=149, Percent_Identity=36.9127516778523, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI310115209, Length=149, Percent_Identity=36.9127516778523, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI310118259, Length=149, Percent_Identity=36.9127516778523, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI310119946, Length=149, Percent_Identity=36.9127516778523, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1787458, Length=310, Percent_Identity=64.8387096774194, Blast_Score=412, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17554704, Length=311, Percent_Identity=45.9807073954984, Blast_Score=293, Evalue=7e-80,
Organism=Caenorhabditis elegans, GI17554702, Length=314, Percent_Identity=45.5414012738853, Blast_Score=293, Evalue=8e-80,
Organism=Caenorhabditis elegans, GI25149168, Length=314, Percent_Identity=45.5414012738853, Blast_Score=293, Evalue=8e-80,
Organism=Caenorhabditis elegans, GI71989924, Length=314, Percent_Identity=45.5414012738853, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17570245, Length=342, Percent_Identity=34.5029239766082, Blast_Score=210, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6320946, Length=319, Percent_Identity=43.5736677115987, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6321776, Length=316, Percent_Identity=45.8860759493671, Blast_Score=268, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6319403, Length=320, Percent_Identity=43.75, Blast_Score=266, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=43.8775510204082, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6324511, Length=109, Percent_Identity=40.3669724770642, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21355239, Length=314, Percent_Identity=45.859872611465, Blast_Score=280, Evalue=8e-76,
Organism=Drosophila melanogaster, GI45551540, Length=335, Percent_Identity=42.9850746268657, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24651458, Length=358, Percent_Identity=32.9608938547486, Blast_Score=204, Evalue=7e-53,
Organism=Drosophila melanogaster, GI24651456, Length=358, Percent_Identity=32.9608938547486, Blast_Score=204, Evalue=7e-53,
Organism=Drosophila melanogaster, GI281362873, Length=358, Percent_Identity=32.9608938547486, Blast_Score=204, Evalue=9e-53,
Organism=Drosophila melanogaster, GI24651454, Length=358, Percent_Identity=32.9608938547486, Blast_Score=204, Evalue=9e-53,
Organism=Drosophila melanogaster, GI24651462, Length=216, Percent_Identity=37.037037037037, Blast_Score=147, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24651464, Length=216, Percent_Identity=37.037037037037, Blast_Score=147, Evalue=6e-36,
Organism=Drosophila melanogaster, GI45552010, Length=216, Percent_Identity=37.037037037037, Blast_Score=147, Evalue=7e-36,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34328; Mature: 34197

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEGLMVFTGNANPKLAEAVVKHLGIPLGKALVGRFSDGEVQVEIQENVRGKHVFILQS
CCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEEECCCCCCCEEEEEEC
TCAPTNDNLMELMVMVDALKRASARRITAAIPYFGYARQDRRPRSARVAITAKVVANMLE
CCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHEEEEHHHHHHHHHH
VAGVERVLTMDLHADQIQGFFDIPVDNIYASPILLEDLRKKNYEDLLVVSPDVGGVVRAR
HHCHHHEEEECCCHHHHCCEEECCHHHHCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHH
ALAKQLGVDLAIIDKRRPKANVAEVMNIIGEVDGRNCVIMDDMIDTGGTLCKAAQVLKER
HHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHC
GAKQVFSYCTHPVLSGGAAARIADSALDEVVVTDTIPLREDAIKCGKIRQLSTAPLLAET
CHHHHHHHHCCHHHCCCHHHHHHHHHHHCEEEECCCCCHHHHHHHCCCCCCCCCHHHHHH
FTRIVRGDSIMSLFAE
HHHHHCCCHHHHHHCC
>Mature Secondary Structure 
SSEGLMVFTGNANPKLAEAVVKHLGIPLGKALVGRFSDGEVQVEIQENVRGKHVFILQS
CCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEEECCCCCCCEEEEEEC
TCAPTNDNLMELMVMVDALKRASARRITAAIPYFGYARQDRRPRSARVAITAKVVANMLE
CCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHEEEEHHHHHHHHHH
VAGVERVLTMDLHADQIQGFFDIPVDNIYASPILLEDLRKKNYEDLLVVSPDVGGVVRAR
HHCHHHEEEECCCHHHHCCEEECCHHHHCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHH
ALAKQLGVDLAIIDKRRPKANVAEVMNIIGEVDGRNCVIMDDMIDTGGTLCKAAQVLKER
HHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHC
GAKQVFSYCTHPVLSGGAAARIADSALDEVVVTDTIPLREDAIKCGKIRQLSTAPLLAET
CHHHHHHHHCCHHHCCCHHHHHHHHHHHCEEEECCCCCHHHHHHHCCCCCCCCCHHHHHH
FTRIVRGDSIMSLFAE
HHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]