Click here to switch to the map view.

The map label for this gene is lipB

Identifier: 187927287

GI number: 187927287

Start: 198504

End: 199193

Strand: Reverse

Name: lipB

Synonym: Rpic_0179

Alternate gene names: 187927287

Gene position: 199193-198504 (Counterclockwise)

Preceding gene: 187927288

Following gene: 187927286

Centisome position: 5.05

GC content: 64.93

Gene sequence:

>690_bases
ATGATTCCGATTGACATCGTCGAACGCGGCCTGCAGGACTACGCCGCTTGCTTCGACGAAATGCAGACATTTACCGCGCG
GCGCGGGCCCGATACTCCTGACACGATCTGGCTGGTCGAACACCCGCCGGTGTTCACGCTCGGCCTGGCCGGTGATCCCG
CGCATCTGTTGGCCCCCGGCAACATTCCGCTGGTGAAAGTCGACCGTGGCGGTCAGATCACTTATCACGGCCCTGGCCAA
GTTGTCGCTTACCTTTTGCTGGATTTGCGCCGACGCGGGCTGTTCGTGAAGGCGCTGGTCGATGCCATCGAGGCGGCCGT
CATCCAGACGCTCGCCACGTATAATGTCGCCTCCGAACGCAAGGCTGGCGCCCCCGGCATCTACCTGTCGAGCGGGCCGC
ATGCCGGCGCCAAGATCGCCGCGCTGGGGCTGAAGATCCGCAACGGCTGCAGCTACCACGGCGTGAGCCTGAACCTGGCG
ATGGACCTGTCGCCCTTCACGCAGATCAACCCGTGTGGCTATGCCGGCCTGGAAACCGTCGATATGGTGACCGCCGGCGC
CCGTGATGCGTCCGGACGCGCCGTCGATGCGAACGACTGGCAAACCGTTTCCCGCGACCTGGCCAATGCGCTGGTCGCTC
AATTGCAGCAACGCGCGCAAGCGCACCCCGCCGAGGCCGCTACCGCATAG

Upstream 100 bases:

>100_bases
CGATGCCTCCCATCGCGCCGGCATTTTTTTGCCCGACCCTGCGACCGAAGCGGGCAAAGGCGCACGGCGACATCGACGGC
CCTGCGCTACACTCGCGAGC

Downstream 100 bases:

>100_bases
CGCCCGGATATGCACGAGCCGCAAGCCCGTGCGGAGAACACGATGACCGATTCCGCCGCCGGCGCCACTGAAGTCGCCAC
CCCTGCCACCCCGTCGAACA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MIPIDIVERGLQDYAACFDEMQTFTARRGPDTPDTIWLVEHPPVFTLGLAGDPAHLLAPGNIPLVKVDRGGQITYHGPGQ
VVAYLLLDLRRRGLFVKALVDAIEAAVIQTLATYNVASERKAGAPGIYLSSGPHAGAKIAALGLKIRNGCSYHGVSLNLA
MDLSPFTQINPCGYAGLETVDMVTAGARDASGRAVDANDWQTVSRDLANALVAQLQQRAQAHPAEAATA

Sequences:

>Translated_229_residues
MIPIDIVERGLQDYAACFDEMQTFTARRGPDTPDTIWLVEHPPVFTLGLAGDPAHLLAPGNIPLVKVDRGGQITYHGPGQ
VVAYLLLDLRRRGLFVKALVDAIEAAVIQTLATYNVASERKAGAPGIYLSSGPHAGAKIAALGLKIRNGCSYHGVSLNLA
MDLSPFTQINPCGYAGLETVDMVTAGARDASGRAVDANDWQTVSRDLANALVAQLQQRAQAHPAEAATA
>Mature_229_residues
MIPIDIVERGLQDYAACFDEMQTFTARRGPDTPDTIWLVEHPPVFTLGLAGDPAHLLAPGNIPLVKVDRGGQITYHGPGQ
VVAYLLLDLRRRGLFVKALVDAIEAAVIQTLATYNVASERKAGAPGIYLSSGPHAGAKIAALGLKIRNGCSYHGVSLNLA
MDLSPFTQINPCGYAGLETVDMVTAGARDASGRAVDANDWQTVSRDLANALVAQLQQRAQAHPAEAATA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Escherichia coli, GI87081767, Length=175, Percent_Identity=52.5714285714286, Blast_Score=191, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6323268, Length=130, Percent_Identity=33.0769230769231, Blast_Score=82, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21355483, Length=238, Percent_Identity=27.7310924369748, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_RALPJ (B2UE02)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001897774.1
- ProteinModelPortal:   B2UE02
- SMR:   B2UE02
- GeneID:   6289348
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0179
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- ProtClustDB:   PRK14343
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24251; Mature: 24251

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 172-172

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPIDIVERGLQDYAACFDEMQTFTARRGPDTPDTIWLVEHPPVFTLGLAGDPAHLLAPG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCCCEEECCC
NIPLVKVDRGGQITYHGPGQVVAYLLLDLRRRGLFVKALVDAIEAAVIQTLATYNVASER
CCCEEEECCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHC
KAGAPGIYLSSGPHAGAKIAALGLKIRNGCSYHGVSLNLAMDLSPFTQINPCGYAGLETV
CCCCCEEEECCCCCCCCEEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHH
DMVTAGARDASGRAVDANDWQTVSRDLANALVAQLQQRAQAHPAEAATA
HHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIPIDIVERGLQDYAACFDEMQTFTARRGPDTPDTIWLVEHPPVFTLGLAGDPAHLLAPG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCCCEEECCC
NIPLVKVDRGGQITYHGPGQVVAYLLLDLRRRGLFVKALVDAIEAAVIQTLATYNVASER
CCCEEEECCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHC
KAGAPGIYLSSGPHAGAKIAALGLKIRNGCSYHGVSLNLAMDLSPFTQINPCGYAGLETV
CCCCCEEEECCCCCCCCEEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHH
DMVTAGARDASGRAVDANDWQTVSRDLANALVAQLQQRAQAHPAEAATA
HHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA