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The map label for this gene is dmlR [H]

Identifier: 187927177

GI number: 187927177

Start: 81169

End: 82083

Strand: Reverse

Name: dmlR [H]

Synonym: Rpic_0067

Alternate gene names: 187927177

Gene position: 82083-81169 (Counterclockwise)

Preceding gene: 187927178

Following gene: 187927169

Centisome position: 2.08

GC content: 69.51

Gene sequence:

>915_bases
ATGATCCGCTTCGACGACCTCACCATCCTCGTGCTGGCCGTGGATAACGGCAGCCTGTCGGCGGCCGCACGCGTGTTGAA
CATTGCACCCGCGGCAGCAAGTGCAGCCGTCAAGCGGTTGGAGGCGGAACTCGGCGTGCGGCTGCTGGCGCGCTCCACGC
GCAGCCTGGCGCTCACGCAGGAAGGCGAGCGCTATCTGCAACACGCACGCCGTGCGCTGGCCGATCTGCAGGCCGGGCAG
GATGCGCTCGCCCTTGGACGCCAAGCCATCGGCGGCGATGTGTCGCTGTCGATCCCGTCAGACCTCGGGCGCAACGTGCT
CACTGGCTGGCTCGACGACTTCCTCACCGAGCACCCAGCGGTGGCGCTTCAGGTGCGCATCGGCGACCGGCTGGTCGACA
TGTTCCGCCAGCGCGTCGATGCCGCGGTGCGCTATGGCGAGCCGGAGGATTCGTCCCTCGTGGCGCTGCCGCTGGCGCCG
GACAACCGCCGCGTGCTGTGCGCGGCACCGGCCTACTTTGCGCGGCACGGTGTGCCCAAGACCCCCGACGATCTGCGTCG
GCACAACTGCCTGTTGCTGGCCCTGTCCGACACCACGCACGACCAGTGGACCTTCCACGGCGCGAGCGACGACGGTCAGG
CGCACGTTGTGCGCGTCACCGGCAACCGCATTGCCGACGACGGCGAACTCGTGCGCCGCTGGGCCGTTGCGGGGCATGGC
ATCGCTTACAAGTCGCGGCTGGATGTACTGGCCGATCTGCGCGCCGGCCGGCTGCAAGCCGCGCTGACCGACTGGCGCGG
CGAGGCGGCACCGCTGCATTTGATCTGCACGCACCGCCTCGCGCTGTCGCCCACGCTGATGCGGCTGCGCGACTTCCTGC
AAGAGCGCATCGCCGGTTATCTGGCCGACGCCTGA

Upstream 100 bases:

>100_bases
ATGTGGGATGCGAGTCGAAGGGGCAAGGTGCGGAAAGCCGATGTGCTCGGTAAGATGCCGTCCATCCCCGGTTTTTCCAC
TCCACTCCACACCACGCACG

Downstream 100 bases:

>100_bases
ATCAAAACCCTTCCAGCACGATCTTGCCGCGCGCCTGATTGCTTTCAATAAAGGCTTGTGCGCGCTTGAGGTTGGCGGCA
TTGATGGTGCCGAAGTGCTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MIRFDDLTILVLAVDNGSLSAAARVLNIAPAAASAAVKRLEAELGVRLLARSTRSLALTQEGERYLQHARRALADLQAGQ
DALALGRQAIGGDVSLSIPSDLGRNVLTGWLDDFLTEHPAVALQVRIGDRLVDMFRQRVDAAVRYGEPEDSSLVALPLAP
DNRRVLCAAPAYFARHGVPKTPDDLRRHNCLLLALSDTTHDQWTFHGASDDGQAHVVRVTGNRIADDGELVRRWAVAGHG
IAYKSRLDVLADLRAGRLQAALTDWRGEAAPLHLICTHRLALSPTLMRLRDFLQERIAGYLADA

Sequences:

>Translated_304_residues
MIRFDDLTILVLAVDNGSLSAAARVLNIAPAAASAAVKRLEAELGVRLLARSTRSLALTQEGERYLQHARRALADLQAGQ
DALALGRQAIGGDVSLSIPSDLGRNVLTGWLDDFLTEHPAVALQVRIGDRLVDMFRQRVDAAVRYGEPEDSSLVALPLAP
DNRRVLCAAPAYFARHGVPKTPDDLRRHNCLLLALSDTTHDQWTFHGASDDGQAHVVRVTGNRIADDGELVRRWAVAGHG
IAYKSRLDVLADLRAGRLQAALTDWRGEAAPLHLICTHRLALSPTLMRLRDFLQERIAGYLADA
>Mature_304_residues
MIRFDDLTILVLAVDNGSLSAAARVLNIAPAAASAAVKRLEAELGVRLLARSTRSLALTQEGERYLQHARRALADLQAGQ
DALALGRQAIGGDVSLSIPSDLGRNVLTGWLDDFLTEHPAVALQVRIGDRLVDMFRQRVDAAVRYGEPEDSSLVALPLAP
DNRRVLCAAPAYFARHGVPKTPDDLRRHNCLLLALSDTTHDQWTFHGASDDGQAHVVRVTGNRIADDGELVRRWAVAGHG
IAYKSRLDVLADLRAGRLQAALTDWRGEAAPLHLICTHRLALSPTLMRLRDFLQERIAGYLADA

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=300, Percent_Identity=31.3333333333333, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1789440, Length=260, Percent_Identity=30.7692307692308, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=31.6326530612245, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1789639, Length=304, Percent_Identity=28.9473684210526, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1787128, Length=293, Percent_Identity=27.3037542662116, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=29.9319727891156, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787589, Length=275, Percent_Identity=28.7272727272727, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786448, Length=253, Percent_Identity=29.6442687747036, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789173, Length=259, Percent_Identity=26.6409266409266, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788706, Length=206, Percent_Identity=27.1844660194175, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI157672245, Length=226, Percent_Identity=26.5486725663717, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790011, Length=264, Percent_Identity=24.2424242424242, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787601, Length=181, Percent_Identity=24.3093922651934, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33127; Mature: 33127

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRFDDLTILVLAVDNGSLSAAARVLNIAPAAASAAVKRLEAELGVRLLARSTRSLALTQ
CEEECCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHEEECH
EGERYLQHARRALADLQAGQDALALGRQAIGGDVSLSIPSDLGRNVLTGWLDDFLTEHPA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCC
VALQVRIGDRLVDMFRQRVDAAVRYGEPEDSSLVALPLAPDNRRVLCAAPAYFARHGVPK
EEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEECCHHHHHCCCCC
TPDDLRRHNCLLLALSDTTHDQWTFHGASDDGQAHVVRVTGNRIADDGELVRRWAVAGHG
CHHHHHHCCEEEEEEECCCCCCEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCC
IAYKSRLDVLADLRAGRLQAALTDWRGEAAPLHLICTHRLALSPTLMRLRDFLQERIAGY
EEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHH
LADA
HCCC
>Mature Secondary Structure
MIRFDDLTILVLAVDNGSLSAAARVLNIAPAAASAAVKRLEAELGVRLLARSTRSLALTQ
CEEECCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHEEECH
EGERYLQHARRALADLQAGQDALALGRQAIGGDVSLSIPSDLGRNVLTGWLDDFLTEHPA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCC
VALQVRIGDRLVDMFRQRVDAAVRYGEPEDSSLVALPLAPDNRRVLCAAPAYFARHGVPK
EEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEECCHHHHHCCCCC
TPDDLRRHNCLLLALSDTTHDQWTFHGASDDGQAHVVRVTGNRIADDGELVRRWAVAGHG
CHHHHHHCCEEEEEEECCCCCCEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCC
IAYKSRLDVLADLRAGRLQAALTDWRGEAAPLHLICTHRLALSPTLMRLRDFLQERIAGY
EEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHH
LADA
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]