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The map label for this gene is ydaP [H]

Identifier: 187927178

GI number: 187927178

Start: 82250

End: 84040

Strand: Reverse

Name: ydaP [H]

Synonym: Rpic_0068

Alternate gene names: 187927178

Gene position: 84040-82250 (Counterclockwise)

Preceding gene: 187927179

Following gene: 187927177

Centisome position: 2.13

GC content: 66.39

Gene sequence:

>1791_bases
ATGCAAGCCACCGTCTGCGATTTCCTCGTCGCCCGCCTGTACGAGTGGGGCGTGCGGCGCATCTTTGGTTACCCCGGCGA
CGGCATCAACGGCATGTTCGGGGCCCTGCAGCGCGCCGGCGGCAAGATCGAGTTCGTTCAGGTGCGGCATGAAGAGATGG
CCGCCTTCATGGCATCGGCGCACGCCAAGTTCACCGGTGAGCTTGGCGTGTGCATGGCAACGTCCGGGCCGGGTGCTGCG
CACCTGATCACGGGCCTGTACGACGCTCGGCTCGACCACATGCCGGTGCTGGCGATTACCGGGCAGCAGGCGCGCACCGC
AGTCGGCGGGCACTACCAGCAGGAGGTGGATCTGGCGGCGATGTTCCGCGACGTGGCCGGCGCGTTTGTCCACCAGGCCA
GCATGCCGGCGCAGGTGCGGCACCTGCTGGATCGCGCGGTACGCATCGCCATCGGGCAACGCCGGGTGACGGCGCTCGTG
CTGCCAAACGATCTGCAGGAAATGCCCTACACCGAACCGCCGCGTGCGCATGGCACGGTGCACTCGGGCGTGGGCTACAG
CGCGCCGAGCGTGATCCCGCAAGCCGCCGACCTGGAACGCGCAGCGGACATCCTCAACAGCGGCCGCCGGGTGGCGATGC
TCGTGGGCGCGGGCGCACTCGGAGCCAGCGCGGAAGTGGCGGCCGTGGCGGAAACGCTGGGGGCGGGCGTGGCCAAGGCG
CTGCTCGGCAAGGCCGTGTTGCCCGACGACCTGCCTTATGTGACAGGCGCCATCGGCCTGCTCGGCACCGAGCCGAGCTG
GGAGCTGATGAACCATTGCGATACGCTGTTGATGGTCGGCTCCGGCTTTCCGTACGCGGAGTTCCTGCCCAAGGAAGGCC
AGGCGCGCGGTGTGCAGATCGACCTGGACCCCGGCATGCTGGGGCTGCGCTATCCGATGGAGGTCAACCTCGTGGGCGAC
AGCGCGCAGACGCTGCGTGCACTGCTGCCGCTGCTCCAGCGCAAGACCGATCCCTATTGGCCCGACCGCATTGCACGCTG
GAATGAAAGCTGGTGGAAGACGCTGGAAGACCGCGCATTGCAACCGGCCTATCCCGTCAACCCGCAGCACGTGTTCTGGG
AGCTGTCACCGAAGCTGCCGGACGACGTGATCGTCACGAGCGACTCCGGCACCTGCGCCAACTGGTATGCGCGCGATCTC
AAGTTCAAGCCCGGCATGATGGGCTCGCTATCGGGCGGGCTGGCATCGATGGGGGCGGCGGTGCCGTATGCGATTGCCGC
CAAGTTTGCGCATCCCGAGCGCCCTGTCATCGCACTGGTGGGCGACGGCGCCATGCAGATGAACAACATGGCGGAACTCA
TCACCGTGTCGAAATACTGGAAGCGCTGGAAGTCTTCCAAGTGGATCTGCATGGTGCTCAACAATCAGGATCTGAACGAA
GTCACATGGGAGCAGCGCGTGATGGAAGGCGACCCGAAATTCCCGGCGGCACAGGACATCCCGAACGTGCCGTATCACCG
CTTTGCCGAATTGATCGGGCTGAAGGGCATCTACGTGGACAACCCCGACCAGCTGGCCGGCGCGTGGGACGAAGCGCTCA
ACGCCGACCGGCCCGTGGTGCTGGAAGTGAAGACCGATCCCGATGTGCCGCCGCTGCCGCCCCACGTCACGCTGCAGCAG
GCACGCAACTTTGCGACGGCACTGGCGTATGGCGACCCGGATGCGAGCGGTGTGCTGCGCGGGACGGCACGGCAGGTGCT
GTCGGCGCTGTTTCCGGTGCATAAGGAATAG

Upstream 100 bases:

>100_bases
ACCATGGCCGCGCGCAAGGAAGCGCTGCTGGCCGAGACGGCGAGCTGACCGAGTTGACGGACCCGAGACGGACCCGACCA
CCTGACACCGGACAGACACC

Downstream 100 bases:

>100_bases
GCGCATAGACGTAACGCCCCCGCTCACGCGGTGGCGCGTCGAAGCGTGAGCGCATGTCGACAAGACATGTGGGATGCGAG
TCGAAGGGGCAAGGTGCGGA

Product: thiamine pyrophosphate protein TPP binding domain-containing protein

Products: Acetate; CO2; Ubiquinol-8 [C]

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MQATVCDFLVARLYEWGVRRIFGYPGDGINGMFGALQRAGGKIEFVQVRHEEMAAFMASAHAKFTGELGVCMATSGPGAA
HLITGLYDARLDHMPVLAITGQQARTAVGGHYQQEVDLAAMFRDVAGAFVHQASMPAQVRHLLDRAVRIAIGQRRVTALV
LPNDLQEMPYTEPPRAHGTVHSGVGYSAPSVIPQAADLERAADILNSGRRVAMLVGAGALGASAEVAAVAETLGAGVAKA
LLGKAVLPDDLPYVTGAIGLLGTEPSWELMNHCDTLLMVGSGFPYAEFLPKEGQARGVQIDLDPGMLGLRYPMEVNLVGD
SAQTLRALLPLLQRKTDPYWPDRIARWNESWWKTLEDRALQPAYPVNPQHVFWELSPKLPDDVIVTSDSGTCANWYARDL
KFKPGMMGSLSGGLASMGAAVPYAIAAKFAHPERPVIALVGDGAMQMNNMAELITVSKYWKRWKSSKWICMVLNNQDLNE
VTWEQRVMEGDPKFPAAQDIPNVPYHRFAELIGLKGIYVDNPDQLAGAWDEALNADRPVVLEVKTDPDVPPLPPHVTLQQ
ARNFATALAYGDPDASGVLRGTARQVLSALFPVHKE

Sequences:

>Translated_596_residues
MQATVCDFLVARLYEWGVRRIFGYPGDGINGMFGALQRAGGKIEFVQVRHEEMAAFMASAHAKFTGELGVCMATSGPGAA
HLITGLYDARLDHMPVLAITGQQARTAVGGHYQQEVDLAAMFRDVAGAFVHQASMPAQVRHLLDRAVRIAIGQRRVTALV
LPNDLQEMPYTEPPRAHGTVHSGVGYSAPSVIPQAADLERAADILNSGRRVAMLVGAGALGASAEVAAVAETLGAGVAKA
LLGKAVLPDDLPYVTGAIGLLGTEPSWELMNHCDTLLMVGSGFPYAEFLPKEGQARGVQIDLDPGMLGLRYPMEVNLVGD
SAQTLRALLPLLQRKTDPYWPDRIARWNESWWKTLEDRALQPAYPVNPQHVFWELSPKLPDDVIVTSDSGTCANWYARDL
KFKPGMMGSLSGGLASMGAAVPYAIAAKFAHPERPVIALVGDGAMQMNNMAELITVSKYWKRWKSSKWICMVLNNQDLNE
VTWEQRVMEGDPKFPAAQDIPNVPYHRFAELIGLKGIYVDNPDQLAGAWDEALNADRPVVLEVKTDPDVPPLPPHVTLQQ
ARNFATALAYGDPDASGVLRGTARQVLSALFPVHKE
>Mature_596_residues
MQATVCDFLVARLYEWGVRRIFGYPGDGINGMFGALQRAGGKIEFVQVRHEEMAAFMASAHAKFTGELGVCMATSGPGAA
HLITGLYDARLDHMPVLAITGQQARTAVGGHYQQEVDLAAMFRDVAGAFVHQASMPAQVRHLLDRAVRIAIGQRRVTALV
LPNDLQEMPYTEPPRAHGTVHSGVGYSAPSVIPQAADLERAADILNSGRRVAMLVGAGALGASAEVAAVAETLGAGVAKA
LLGKAVLPDDLPYVTGAIGLLGTEPSWELMNHCDTLLMVGSGFPYAEFLPKEGQARGVQIDLDPGMLGLRYPMEVNLVGD
SAQTLRALLPLLQRKTDPYWPDRIARWNESWWKTLEDRALQPAYPVNPQHVFWELSPKLPDDVIVTSDSGTCANWYARDL
KFKPGMMGSLSGGLASMGAAVPYAIAAKFAHPERPVIALVGDGAMQMNNMAELITVSKYWKRWKSSKWICMVLNNQDLNE
VTWEQRVMEGDPKFPAAQDIPNVPYHRFAELIGLKGIYVDNPDQLAGAWDEALNADRPVVLEVKTDPDVPPLPPHVTLQQ
ARNFATALAYGDPDASGVLRGTARQVLSALFPVHKE

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=24.2049469964664, Blast_Score=135, Evalue=1e-31,
Organism=Escherichia coli, GI1787096, Length=569, Percent_Identity=34.2706502636204, Blast_Score=289, Evalue=4e-79,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=30.0353356890459, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1790104, Length=568, Percent_Identity=26.5845070422535, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1786717, Length=557, Percent_Identity=27.1095152603232, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1788716, Length=567, Percent_Identity=21.8694885361552, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17531301, Length=580, Percent_Identity=24.6551724137931, Blast_Score=89, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17531299, Length=580, Percent_Identity=24.6551724137931, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323755, Length=565, Percent_Identity=26.5486725663717, Blast_Score=156, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6320816, Length=566, Percent_Identity=21.3780918727915, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19922626, Length=572, Percent_Identity=24.8251748251748, Blast_Score=117, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2 [C]

Molecular weight: Translated: 64693; Mature: 64693

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQATVCDFLVARLYEWGVRRIFGYPGDGINGMFGALQRAGGKIEFVQVRHEEMAAFMASA
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHH
HAKFTGELGVCMATSGPGAAHLITGLYDARLDHMPVLAITGQQARTAVGGHYQQEVDLAA
HHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCHHHHHHHHH
MFRDVAGAFVHQASMPAQVRHLLDRAVRIAIGQRRVTALVLPNDLQEMPYTEPPRAHGTV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCCCCCCCCCCE
HSGVGYSAPSVIPQAADLERAADILNSGRRVAMLVGAGALGASAEVAAVAETLGAGVAKA
ECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LLGKAVLPDDLPYVTGAIGLLGTEPSWELMNHCDTLLMVGSGFPYAEFLPKEGQARGVQI
HHCCCCCCCCCCHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCCHHHHCCCCCCCCCEEE
DLDPGMLGLRYPMEVNLVGDSAQTLRALLPLLQRKTDPYWPDRIARWNESWWKTLEDRAL
ECCCCCEEEECCEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHCCC
QPAYPVNPQHVFWELSPKLPDDVIVTSDSGTCANWYARDLKFKPGMMGSLSGGLASMGAA
CCCCCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHH
VPYAIAAKFAHPERPVIALVGDGAMQMNNMAELITVSKYWKRWKSSKWICMVLNNQDLNE
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHH
VTWEQRVMEGDPKFPAAQDIPNVPYHRFAELIGLKGIYVDNPDQLAGAWDEALNADRPVV
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCCEE
LEVKTDPDVPPLPPHVTLQQARNFATALAYGDPDASGVLRGTARQVLSALFPVHKE
EEEECCCCCCCCCCCCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQATVCDFLVARLYEWGVRRIFGYPGDGINGMFGALQRAGGKIEFVQVRHEEMAAFMASA
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHH
HAKFTGELGVCMATSGPGAAHLITGLYDARLDHMPVLAITGQQARTAVGGHYQQEVDLAA
HHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCHHHHHHHHH
MFRDVAGAFVHQASMPAQVRHLLDRAVRIAIGQRRVTALVLPNDLQEMPYTEPPRAHGTV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCCCCCCCCCCE
HSGVGYSAPSVIPQAADLERAADILNSGRRVAMLVGAGALGASAEVAAVAETLGAGVAKA
ECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LLGKAVLPDDLPYVTGAIGLLGTEPSWELMNHCDTLLMVGSGFPYAEFLPKEGQARGVQI
HHCCCCCCCCCCHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCCHHHHCCCCCCCCCEEE
DLDPGMLGLRYPMEVNLVGDSAQTLRALLPLLQRKTDPYWPDRIARWNESWWKTLEDRAL
ECCCCCEEEECCEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHCCC
QPAYPVNPQHVFWELSPKLPDDVIVTSDSGTCANWYARDLKFKPGMMGSLSGGLASMGAA
CCCCCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHH
VPYAIAAKFAHPERPVIALVGDGAMQMNNMAELITVSKYWKRWKSSKWICMVLNNQDLNE
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHH
VTWEQRVMEGDPKFPAAQDIPNVPYHRFAELIGLKGIYVDNPDQLAGAWDEALNADRPVV
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCCEE
LEVKTDPDVPPLPPHVTLQQARNFATALAYGDPDASGVLRGTARQVLSALFPVHKE
EEEECCCCCCCCCCCCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0022 {lysolecithin}} 0.0017 {lecithin}} 0.0012 {thiamine} 0.03 {sodium} 2 {ferricyanide}} 11 {pyruvate}} 10 {pyruvate}} [C]

Substrates: H2O; Pyruvate; Ubiquinone-8 [C]

Specific reaction: H2O + Pyruvate + Ubiquinone-8 --> Acetate + CO2 + Ubiquinol-8 [C]

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]