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The map label for this gene is ykpB [H]

Identifier: 187927169

GI number: 187927169

Start: 72444

End: 73409

Strand: Reverse

Name: ykpB [H]

Synonym: Rpic_0059

Alternate gene names: 187927169

Gene position: 73409-72444 (Counterclockwise)

Preceding gene: 187927177

Following gene: 187927162

Centisome position: 1.86

GC content: 67.49

Gene sequence:

>966_bases
ATGACGACAGCAATGCGGATTCTGGTGCTTGGCGCCGGTGGCACCGGCGGTTATTTTGGCGGGCGGCTCGCCCAGGCCGG
CGCGGACGTGACGTTCCTGGTGCGCCCTGCCCGCGCGGCGCGGTTGCGCCAACACGGTCTCGTGATCCGCAGCCCGCACG
GCGATGCGCAATTGCCCGTGCAGACCATCACCGCGGACCAGGCCACGGCTTGGGGCCGCGCCGACCTGGTGCTGCTGAGC
TGCAAGGCCTACGACCTGGAAGACGCCATCACCGCGATCCGCCCCGTGGTCGGCGAGCGCACGGTGGTGCTGCCGGTGCT
CAATGGGCTGGCGCATCTGGAACGGCTGGATGCGGCGTTTGGTGAATCGCGCGTGCTGGGTGGGCTGTGCCACATCAGTG
CGACGAGTGCGCCGGATGGTGCCGTGCTGCATCTGAGCAACGGCCAGGGCCCCGCCGTGCACTCGATCACCTTTGGCGAG
CGCACACCGCATGCGCCGCGCGAGCGCACCGAGGCGATTCGCGACGTGTTTGCCACGGCCAACTTTGATTCCGTGCTGGC
CGAGAACGTCATGCAGGACATGTGGGAGAAATTCACGTTCCTCACTTCGCTGGCGGCAATGACGTGCCTGATGCGGGCGA
CGGTGGGCGAGATTGTTGCCACGGACGAGGGCGCGGCGCTGAACGAGGCGATGTTCAACGCGTGCGAGCGTGTGGCTGCG
GCGTCGGGGTATCCGAATCGGCAGGCTGCGCGCGAACGGGGGCTCAATGTGCTGACGCAGGCTGGGTCGCCGCTGACGGC
ATCGATGCTGCGGGATCTGGAGAATGGCGCGCGGGTGGAGGCGGATCACATCGTGGGGGACATGCTGCGCCGTGGGCGGG
CGTTGGGGACGGATGTTTCGCTGCTGCGGGTGGCGTTTGCGCATTTGCAGGCTTATCAGCAGCGGTTGTTGCGGGCGGCT
GTTTGA

Upstream 100 bases:

>100_bases
GGAAAATCAGCATTTTAGCGCGCGCGGGGCGCCGTCTGCAGGGTCACGCGAACAGGCCTAGAATCGTTTGCCTATCTTCT
GCTCGATGGAAACGAACCCA

Downstream 100 bases:

>100_bases
TTGTTTTGTGTTTGTGGTTCATCCCTGGTTTCATCCCCTGCCGGGGCTGACTCACTTTCTTTGTCTTGCCAAAGAAAGTA
AGCAAAGAAAGGCGCGCCCG

Product: 2-dehydropantoate 2-reductase

Products: Nicotinamide adenine dinucleotide phosphate; (R)-Pantoate [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTTAMRILVLGAGGTGGYFGGRLAQAGADVTFLVRPARAARLRQHGLVIRSPHGDAQLPVQTITADQATAWGRADLVLLS
CKAYDLEDAITAIRPVVGERTVVLPVLNGLAHLERLDAAFGESRVLGGLCHISATSAPDGAVLHLSNGQGPAVHSITFGE
RTPHAPRERTEAIRDVFATANFDSVLAENVMQDMWEKFTFLTSLAAMTCLMRATVGEIVATDEGAALNEAMFNACERVAA
ASGYPNRQAARERGLNVLTQAGSPLTASMLRDLENGARVEADHIVGDMLRRGRALGTDVSLLRVAFAHLQAYQQRLLRAA
V

Sequences:

>Translated_321_residues
MTTAMRILVLGAGGTGGYFGGRLAQAGADVTFLVRPARAARLRQHGLVIRSPHGDAQLPVQTITADQATAWGRADLVLLS
CKAYDLEDAITAIRPVVGERTVVLPVLNGLAHLERLDAAFGESRVLGGLCHISATSAPDGAVLHLSNGQGPAVHSITFGE
RTPHAPRERTEAIRDVFATANFDSVLAENVMQDMWEKFTFLTSLAAMTCLMRATVGEIVATDEGAALNEAMFNACERVAA
ASGYPNRQAARERGLNVLTQAGSPLTASMLRDLENGARVEADHIVGDMLRRGRALGTDVSLLRVAFAHLQAYQQRLLRAA
V
>Mature_320_residues
TTAMRILVLGAGGTGGYFGGRLAQAGADVTFLVRPARAARLRQHGLVIRSPHGDAQLPVQTITADQATAWGRADLVLLSC
KAYDLEDAITAIRPVVGERTVVLPVLNGLAHLERLDAAFGESRVLGGLCHISATSAPDGAVLHLSNGQGPAVHSITFGER
TPHAPRERTEAIRDVFATANFDSVLAENVMQDMWEKFTFLTSLAAMTCLMRATVGEIVATDEGAALNEAMFNACERVAAA
SGYPNRQAARERGLNVLTQAGSPLTASMLRDLENGARVEADHIVGDMLRRGRALGTDVSLLRVAFAHLQAYQQRLLRAAV

Specific function: Catalyzes The NADPH-Dependent Reduction Of Ketopantoate Into Pantoic Acid. [C]

COG id: COG1893

COG function: function code H; Ketopantoate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ketopantoate reductase family [H]

Homologues:

Organism=Escherichia coli, GI1786627, Length=301, Percent_Identity=23.2558139534884, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR003710
- InterPro:   IPR013752
- InterPro:   IPR013332
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02558 ApbA; PF08546 ApbA_C [H]

EC number: 1.1.1.169 [C]

Molecular weight: Translated: 34201; Mature: 34069

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAMRILVLGAGGTGGYFGGRLAQAGADVTFLVRPARAARLRQHGLVIRSPHGDAQLPV
CCCEEEEEEEECCCCCCCCCCHHHHCCCCEEEEECCHHHHHHHHCCCEEECCCCCCCCCC
QTITADQATAWGRADLVLLSCKAYDLEDAITAIRPVVGERTVVLPVLNGLAHLERLDAAF
EEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHH
GESRVLGGLCHISATSAPDGAVLHLSNGQGPAVHSITFGERTPHAPRERTEAIRDVFATA
CCHHHHHHHEEEECCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHC
NFDSVLAENVMQDMWEKFTFLTSLAAMTCLMRATVGEIVATDEGAALNEAMFNACERVAA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHH
ASGYPNRQAARERGLNVLTQAGSPLTASMLRDLENGARVEADHIVGDMLRRGRALGTDVS
HCCCCCHHHHHHHCCHHHHHCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHH
LLRVAFAHLQAYQQRLLRAAV
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTAMRILVLGAGGTGGYFGGRLAQAGADVTFLVRPARAARLRQHGLVIRSPHGDAQLPV
CCEEEEEEEECCCCCCCCCCHHHHCCCCEEEEECCHHHHHHHHCCCEEECCCCCCCCCC
QTITADQATAWGRADLVLLSCKAYDLEDAITAIRPVVGERTVVLPVLNGLAHLERLDAAF
EEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHH
GESRVLGGLCHISATSAPDGAVLHLSNGQGPAVHSITFGERTPHAPRERTEAIRDVFATA
CCHHHHHHHEEEECCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHC
NFDSVLAENVMQDMWEKFTFLTSLAAMTCLMRATVGEIVATDEGAALNEAMFNACERVAA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHH
ASGYPNRQAARERGLNVLTQAGSPLTASMLRDLENGARVEADHIVGDMLRRGRALGTDVS
HCCCCCHHHHHHHCCHHHHHCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHH
LLRVAFAHLQAYQQRLLRAAV
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.0 {NADPH}} [C]

Substrates: 2-Dehydropantoate; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: 2-Dehydropantoate + H+ + Nicotinamide adenine dinucleotide phosphate - reduced --> Nicotinamide adenine dinucleotide phosphate + (R)-Pantoate [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]