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The map label for this gene is pyrE [H]

Identifier: 187927151

GI number: 187927151

Start: 53067

End: 53744

Strand: Reverse

Name: pyrE [H]

Synonym: Rpic_0041

Alternate gene names: 187927151

Gene position: 53744-53067 (Counterclockwise)

Preceding gene: 187927154

Following gene: 187927146

Centisome position: 1.36

GC content: 63.72

Gene sequence:

>678_bases
ATGAGCCAAAACAGCGAGTTGCGCCAATCCTTCATCCGCTTTGCGGTGGAGGCCGGCGTGCTGTCATTCGGGGAATTCGT
GACCAAGGCGGGGCGGACGTCGCCCTATTTCTTTAACGCCGGCAAGTTCGCAGACGGCGCCCTGCTGGGCCAAGTCGCGC
AATTCTATGCGAAAACCCTGCTGGACTCGGGTGTCGAGTTCGACATGCTGTTCGGGCCGGCCTACAAGGGCATCACGCTC
GCGTCGGCCACCGCCGTAGCCTTGGCGGGCCTGGGCCGGAATGTGGGCTTTGCGTACAACCGCAAGGAAGCCAAGGACCA
CGGCGAAGGCGGCAGCCTGGTCGGCGCGAAGCTGCAGGGCCGGGTGGTGATCGTCGATGACGTGATCTCTGCCGGCACCT
CGGTGCGCGAGTCCGTCGAGCTGATTCGCGCCGCCGGCGCGACCCCGGCTGCTGTGCTGATCCTGATGGATCGGATGGAG
CGCAGCGGCAACGCCGTGGATATCGGCGAGCGCTCTGCGGTGCAGGATGTGCAGGCGCAGTACGGCATGCCGGTGGTGTC
GATTGCGAATCTGGATGATTTGCTGGGGTATCTGGATAACGCTGGCGACCCGGCGCTGGCTGGGTATCGGGAAAAGGCTG
CTGCTTATCGGGATAAATACGGCGTTAGCGCGATCTGA

Upstream 100 bases:

>100_bases
AATCAAAAAAGCCTTTGCTGGCAGCGGAGCGAACGCCGTTGAGGTTGGCGGAAATGATGCGTAACATGGCGGGACTTTTT
TGCGGACCAGAGAAAAAAAG

Downstream 100 bases:

>100_bases
GTTTTTGCCGCGTTAAAAAACCCCGGTGGATGTTGTTCGCCGGGGTTTTGTTTTTTGTGGCTCATCCTCCTGCCGCGGCC
GATGCGAGGGATGCACCATC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MSQNSELRQSFIRFAVEAGVLSFGEFVTKAGRTSPYFFNAGKFADGALLGQVAQFYAKTLLDSGVEFDMLFGPAYKGITL
ASATAVALAGLGRNVGFAYNRKEAKDHGEGGSLVGAKLQGRVVIVDDVISAGTSVRESVELIRAAGATPAAVLILMDRME
RSGNAVDIGERSAVQDVQAQYGMPVVSIANLDDLLGYLDNAGDPALAGYREKAAAYRDKYGVSAI

Sequences:

>Translated_225_residues
MSQNSELRQSFIRFAVEAGVLSFGEFVTKAGRTSPYFFNAGKFADGALLGQVAQFYAKTLLDSGVEFDMLFGPAYKGITL
ASATAVALAGLGRNVGFAYNRKEAKDHGEGGSLVGAKLQGRVVIVDDVISAGTSVRESVELIRAAGATPAAVLILMDRME
RSGNAVDIGERSAVQDVQAQYGMPVVSIANLDDLLGYLDNAGDPALAGYREKAAAYRDKYGVSAI
>Mature_224_residues
SQNSELRQSFIRFAVEAGVLSFGEFVTKAGRTSPYFFNAGKFADGALLGQVAQFYAKTLLDSGVEFDMLFGPAYKGITLA
SATAVALAGLGRNVGFAYNRKEAKDHGEGGSLVGAKLQGRVVIVDDVISAGTSVRESVELIRAAGATPAAVLILMDRMER
SGNAVDIGERSAVQDVQAQYGMPVVSIANLDDLLGYLDNAGDPALAGYREKAAAYRDKYGVSAI

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=180, Percent_Identity=28.3333333333333, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1790073, Length=216, Percent_Identity=54.1666666666667, Blast_Score=226, Evalue=8e-61,
Organism=Caenorhabditis elegans, GI17554964, Length=203, Percent_Identity=24.6305418719212, Blast_Score=63, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6323530, Length=222, Percent_Identity=42.7927927927928, Blast_Score=173, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6323927, Length=224, Percent_Identity=39.7321428571429, Blast_Score=165, Evalue=7e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23701; Mature: 23570

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNSELRQSFIRFAVEAGVLSFGEFVTKAGRTSPYFFNAGKFADGALLGQVAQFYAKTL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHH
LDSGVEFDMLFGPAYKGITLASATAVALAGLGRNVGFAYNRKEAKDHGEGGSLVGAKLQG
HHCCCCEEEEECCCCCCEEHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCEEEEEECC
RVVIVDDVISAGTSVRESVELIRAAGATPAAVLILMDRMERSGNAVDIGERSAVQDVQAQ
CEEEEECHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHH
YGMPVVSIANLDDLLGYLDNAGDPALAGYREKAAAYRDKYGVSAI
HCCCEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SQNSELRQSFIRFAVEAGVLSFGEFVTKAGRTSPYFFNAGKFADGALLGQVAQFYAKTL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHH
LDSGVEFDMLFGPAYKGITLASATAVALAGLGRNVGFAYNRKEAKDHGEGGSLVGAKLQG
HHCCCCEEEEECCCCCCEEHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCEEEEEECC
RVVIVDDVISAGTSVRESVELIRAAGATPAAVLILMDRMERSGNAVDIGERSAVQDVQAQ
CEEEEECHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHH
YGMPVVSIANLDDLLGYLDNAGDPALAGYREKAAAYRDKYGVSAI
HCCCEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]