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The map label for this gene is metK

Identifier: 187927146

GI number: 187927146

Start: 47913

End: 49103

Strand: Reverse

Name: metK

Synonym: Rpic_0036

Alternate gene names: 187927146

Gene position: 49103-47913 (Counterclockwise)

Preceding gene: 187927151

Following gene: 187927144

Centisome position: 1.25

GC content: 66.16

Gene sequence:

>1191_bases
GTGTCAAACGACTTCCTCTTCACCTCGGAGTCGGTCTCCGAGGGCCATCCCGACAAGGTCGCCGACCAGATTTCCGACGC
CATCCTGGACGCCATCCTCGCCCAGGACAAATATGCGCGCGTCGCAGCAGAAACGCTGTGCAACACCGGCCTGGTGGTGC
TCGCGGGTGAAATCACCACGACGGCCAACGTCGACTACATCCACGTTGCGCGCGAAACCATCAAGCGCATCGGCTACGAC
AACACCGAATACGGCATCGACTACAAGGGCTGTGCCGTCCTCGTCGCTTACGACAAGCAATCGCCGGACATTGCCCAGGG
CGTCGACCGCGCATCGGACGACTACCTCAACCAGGGCGCCGGCGACCAGGGCCTGATGTTCGGCTACGCGTGCGATGAAA
CGCCCGAGCTGATGCCCTTCCCGATCTACTACGCGCACCGCCTGGTCGAGCGCCAGTCGCAACTGCGCCGCGACGGCCGC
CTGCCCTGGCTGCGTCCGGACGCCAAGTCGCAGGTGACGGTGCGTTATGTGGATGGCAAGCCGCACAGCGTCGATACCGT
GGTGCTGTCCACCCAGCACGCGCCGGAAATGTCGCAAGAGGCCATCCGCGAAGCCGTGATCGAAGAGATCATCAAGCCGG
TGCTCCCGTCGCACATGCTGGCCGAGACCAAGTACCTGGTGAACCCGACCGGCCGCTTCGTCATCGGCGGCCCGCAAGGC
GACTGCGGCCTGACCGGCCGCAAGATCATCGTCGACACGTACGGCGGCGCCGCCCCGCACGGCGGCGGTGCGTTCTCGGG
CAAGGACCCGTCGAAGGTCGACCGCTCGGCGGCGTATGCCGCGCGCTACGTGGCCAAGAACGTGGTGGCCGCCGGTCTGG
CGCGCCAGTGCCAGGTGCAGGTGAGCTACGCGATTGGCGTCGCACGCCCGATCAACATCACGGTGTACACGGAAGGCACG
GGCGTGATCCCGGACGAGCAGATCGCCAAGCTCGTGCAGGAGCACTTCGACCTGCGCCCGAAGGGCATTGTGCAGATGCT
GGACCTGCTGCGCCCGGTCTACGAAAAGACCGCCGCGTATGGCCACTTCGGCCGCGAAGAGCCCGAGTTCAGCTGGGAAG
CCACCGACAAGGTCCTGCTGCTGCGCGAAGCGGCCGGCCTGGCCGGCGAGCCGGCCAAGGCGTTTGCCTGA

Upstream 100 bases:

>100_bases
TTGGCCACAAACCTCGGCAAGGTTGCTAAATACCGCTAAAGCGTCGCCGGCCGTGGCTCAACGGATTGGCAACGTGATAG
CCAATCTGGAGCTAACAGCC

Downstream 100 bases:

>100_bases
GGCCACCCTGCTGCAGACAAGAAACCGCCTCTCGGGGCGGTTTTTTGTTGCGTGCTTGGTAATACCGCGCTCACGGCATC
TTGCTGAGAATGCCGTTCAT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSNDFLFTSESVSEGHPDKVADQISDAILDAILAQDKYARVAAETLCNTGLVVLAGEITTTANVDYIHVARETIKRIGYD
NTEYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGAGDQGLMFGYACDETPELMPFPIYYAHRLVERQSQLRRDGR
LPWLRPDAKSQVTVRYVDGKPHSVDTVVLSTQHAPEMSQEAIREAVIEEIIKPVLPSHMLAETKYLVNPTGRFVIGGPQG
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQVSYAIGVARPINITVYTEGT
GVIPDEQIAKLVQEHFDLRPKGIVQMLDLLRPVYEKTAAYGHFGREEPEFSWEATDKVLLLREAAGLAGEPAKAFA

Sequences:

>Translated_396_residues
MSNDFLFTSESVSEGHPDKVADQISDAILDAILAQDKYARVAAETLCNTGLVVLAGEITTTANVDYIHVARETIKRIGYD
NTEYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGAGDQGLMFGYACDETPELMPFPIYYAHRLVERQSQLRRDGR
LPWLRPDAKSQVTVRYVDGKPHSVDTVVLSTQHAPEMSQEAIREAVIEEIIKPVLPSHMLAETKYLVNPTGRFVIGGPQG
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQVSYAIGVARPINITVYTEGT
GVIPDEQIAKLVQEHFDLRPKGIVQMLDLLRPVYEKTAAYGHFGREEPEFSWEATDKVLLLREAAGLAGEPAKAFA
>Mature_395_residues
SNDFLFTSESVSEGHPDKVADQISDAILDAILAQDKYARVAAETLCNTGLVVLAGEITTTANVDYIHVARETIKRIGYDN
TEYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGAGDQGLMFGYACDETPELMPFPIYYAHRLVERQSQLRRDGRL
PWLRPDAKSQVTVRYVDGKPHSVDTVVLSTQHAPEMSQEAIREAVIEEIIKPVLPSHMLAETKYLVNPTGRFVIGGPQGD
CGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQVSYAIGVARPINITVYTEGTG
VIPDEQIAKLVQEHFDLRPKGIVQMLDLLRPVYEKTAAYGHFGREEPEFSWEATDKVLLLREAAGLAGEPAKAFA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=383, Percent_Identity=58.4856396866841, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI5174529, Length=381, Percent_Identity=58.5301837270341, Blast_Score=421, Evalue=1e-118,
Organism=Escherichia coli, GI1789311, Length=383, Percent_Identity=68.9295039164491, Blast_Score=535, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI17538498, Length=376, Percent_Identity=57.7127659574468, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI25145633, Length=395, Percent_Identity=55.1898734177215, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=57.4468085106383, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17551082, Length=378, Percent_Identity=55.8201058201058, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32565905, Length=376, Percent_Identity=51.8617021276596, Blast_Score=356, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI32565911, Length=330, Percent_Identity=55.1515151515151, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI25145635, Length=347, Percent_Identity=52.4495677233429, Blast_Score=348, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI32565903, Length=303, Percent_Identity=54.7854785478548, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=61.9047619047619, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI32565907, Length=38, Percent_Identity=81.5789473684211, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320710, Length=384, Percent_Identity=57.5520833333333, Blast_Score=425, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=58.7301587301587, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI45552159, Length=376, Percent_Identity=55.5851063829787, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580529, Length=376, Percent_Identity=55.5851063829787, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580537, Length=376, Percent_Identity=55.5851063829787, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580533, Length=376, Percent_Identity=55.5851063829787, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580535, Length=376, Percent_Identity=55.5851063829787, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580531, Length=376, Percent_Identity=55.5851063829787, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580527, Length=376, Percent_Identity=55.0531914893617, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580525, Length=376, Percent_Identity=55.0531914893617, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580541, Length=377, Percent_Identity=52.5198938992042, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580539, Length=121, Percent_Identity=58.6776859504132, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_RALPJ (B2UD22)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001897633.1
- ProteinModelPortal:   B2UD22
- SMR:   B2UD22
- GeneID:   6287394
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0036
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 43135; Mature: 43004

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDFLFTSESVSEGHPDKVADQISDAILDAILAQDKYARVAAETLCNTGLVVLAGEITT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANVDYIHVARETIKRIGYDNTEYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGA
ECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCHHHHHCCC
GDQGLMFGYACDETPELMPFPIYYAHRLVERQSQLRRDGRLPWLRPDAKSQVTVRYVDGK
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PHSVDTVVLSTQHAPEMSQEAIREAVIEEIIKPVLPSHMLAETKYLVNPTGRFVIGGPQG
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEECCCCCEEEECCCC
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
VSYAIGVARPINITVYTEGTGVIPDEQIAKLVQEHFDLRPKGIVQMLDLLRPVYEKTAAY
EEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GHFGREEPEFSWEATDKVLLLREAAGLAGEPAKAFA
CCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCHHHCC
>Mature Secondary Structure 
SNDFLFTSESVSEGHPDKVADQISDAILDAILAQDKYARVAAETLCNTGLVVLAGEITT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANVDYIHVARETIKRIGYDNTEYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGA
ECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCHHHHHCCC
GDQGLMFGYACDETPELMPFPIYYAHRLVERQSQLRRDGRLPWLRPDAKSQVTVRYVDGK
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PHSVDTVVLSTQHAPEMSQEAIREAVIEEIIKPVLPSHMLAETKYLVNPTGRFVIGGPQG
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEECCCCCEEEECCCC
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
VSYAIGVARPINITVYTEGTGVIPDEQIAKLVQEHFDLRPKGIVQMLDLLRPVYEKTAAY
EEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GHFGREEPEFSWEATDKVLLLREAAGLAGEPAKAFA
CCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA