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The map label for this gene is miaA [H]

Identifier: 187935269

GI number: 187935269

Start: 1899844

End: 1900773

Strand: Reverse

Name: miaA [H]

Synonym: CLL_A1817

Alternate gene names: 187935269

Gene position: 1900773-1899844 (Counterclockwise)

Preceding gene: 187932933

Following gene: 187934496

Centisome position: 50.02

GC content: 28.6

Gene sequence:

>930_bases
ATGAAACAAAAGATTTTAGTTCTTGGAGGTCCTACAGCTGTTGGGAAAACAGAACTTTCAATAAAACTTGCTGAAAAATT
AAACGGGGAGATACTATCAGCAGATTCAATGCAAATATACAAGAAGATGGATATAGGTTCAGCTAAAGTTACAAAAGAAG
AGATGAGAGATATAAATCATCATATGATAGACATTGTTTCTCCAGAAGAGGAATTTTCAGTTGCAGATTTTAAAAATATA
GGTGAAAAAGCAATAAAAGAAATTATAGCTAAGGAAAAACTTCCTATGATAGTTGGAGGAACAGGACTTTATATAAATTC
TTTAACTTGCAATGTTACTTTTACAGAATCTGAAAAAGATGATGAGTATAGAACTTATCTAGAATCACTTGCAGAAGCTA
ATGGTAATAACTATGTTCATGAAATGCTTAGAGAAATAGATGAAATAAGTTATAGAGATATACATCCTAACAATAGAAAA
AGAGTAATAAGGGCCCTTGAAGTTTATAAAATTTCAGGAAAGCCATTTAGTTCATATAATGCAGGTAATGATTTCTATAA
AACAGATTACCATGTGTTTTATTATGTATTAACTATGGATAGAGAAAAATTATATGATAGGATTAATAAGAGAGTGGATA
TTATGATTGAAAATGGGCTTATTGATGAGTGCATAGAATTAAAAAAACTTGGATATACTTCAAGTATGCAGTCTATGCAA
GGAATCGGGTATAAAGAAATACTTTATTATTTAGATAAAAAAATATCATTATATGAAGCTGTTAATTTGATAAAGCAAGG
TTCAAGAAATTATGCGAAAAGACAATTGACTTGGTTTAGAAGAGATCCTAGATGTACTTTTTTAGATAAGGATGTTTTAT
CTGATAAGGAAATATTAAGTAAAATTGTTGATGACATAACAAATAACTAG

Upstream 100 bases:

>100_bases
GATATATAGACGATCCATTCCATTGTCCTCATGGAAGACCAACCATTATTCAATTTACTAGTACAGATATAGATAAAAAA
TTTAGGAGGATTGTTTAATA

Downstream 100 bases:

>100_bases
GTTTATAATGGTATAAAGAGAATTAATAAAATGGAGGTAATATGGTTTTATGAATAAGCAACAAAATAATCTACAAGACA
TATTTTTAAACAGTGCAAGA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKQKILVLGGPTAVGKTELSIKLAEKLNGEILSADSMQIYKKMDIGSAKVTKEEMRDINHHMIDIVSPEEEFSVADFKNI
GEKAIKEIIAKEKLPMIVGGTGLYINSLTCNVTFTESEKDDEYRTYLESLAEANGNNYVHEMLREIDEISYRDIHPNNRK
RVIRALEVYKISGKPFSSYNAGNDFYKTDYHVFYYVLTMDREKLYDRINKRVDIMIENGLIDECIELKKLGYTSSMQSMQ
GIGYKEILYYLDKKISLYEAVNLIKQGSRNYAKRQLTWFRRDPRCTFLDKDVLSDKEILSKIVDDITNN

Sequences:

>Translated_309_residues
MKQKILVLGGPTAVGKTELSIKLAEKLNGEILSADSMQIYKKMDIGSAKVTKEEMRDINHHMIDIVSPEEEFSVADFKNI
GEKAIKEIIAKEKLPMIVGGTGLYINSLTCNVTFTESEKDDEYRTYLESLAEANGNNYVHEMLREIDEISYRDIHPNNRK
RVIRALEVYKISGKPFSSYNAGNDFYKTDYHVFYYVLTMDREKLYDRINKRVDIMIENGLIDECIELKKLGYTSSMQSMQ
GIGYKEILYYLDKKISLYEAVNLIKQGSRNYAKRQLTWFRRDPRCTFLDKDVLSDKEILSKIVDDITNN
>Mature_309_residues
MKQKILVLGGPTAVGKTELSIKLAEKLNGEILSADSMQIYKKMDIGSAKVTKEEMRDINHHMIDIVSPEEEFSVADFKNI
GEKAIKEIIAKEKLPMIVGGTGLYINSLTCNVTFTESEKDDEYRTYLESLAEANGNNYVHEMLREIDEISYRDIHPNNRK
RVIRALEVYKISGKPFSSYNAGNDFYKTDYHVFYYVLTMDREKLYDRINKRVDIMIENGLIDECIELKKLGYTSSMQSMQ
GIGYKEILYYLDKKISLYEAVNLIKQGSRNYAKRQLTWFRRDPRCTFLDKDVLSDKEILSKIVDDITNN

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=307, Percent_Identity=28.9902280130293, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1790613, Length=290, Percent_Identity=35.8620689655172, Blast_Score=174, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI25144712, Length=302, Percent_Identity=32.7814569536424, Blast_Score=127, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6324848, Length=294, Percent_Identity=32.6530612244898, Blast_Score=146, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24649830, Length=270, Percent_Identity=37.037037037037, Blast_Score=177, Evalue=7e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 35747; Mature: 35747

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKILVLGGPTAVGKTELSIKLAEKLNGEILSADSMQIYKKMDIGSAKVTKEEMRDINH
CCCEEEEEECCCCCCCCCEEEEEHHHCCCCEECCCHHHHHHHHCCCCHHCCHHHHHHHHH
HMIDIVSPEEEFSVADFKNIGEKAIKEIIAKEKLPMIVGGTGLYINSLTCNVTFTESEKD
HEEEEECCHHHCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEEEEEEECCCCCC
DEYRTYLESLAEANGNNYVHEMLREIDEISYRDIHPNNRKRVIRALEVYKISGKPFSSYN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEECCCCCCCCC
AGNDFYKTDYHVFYYVLTMDREKLYDRINKRVDIMIENGLIDECIELKKLGYTSSMQSMQ
CCCCCCCCCEEEEEEEEECCHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHH
GIGYKEILYYLDKKISLYEAVNLIKQGSRNYAKRQLTWFRRDPRCTFLDKDVLSDKEILS
CCCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEECHHHCCCHHHHH
KIVDDITNN
HHHHHHCCC
>Mature Secondary Structure
MKQKILVLGGPTAVGKTELSIKLAEKLNGEILSADSMQIYKKMDIGSAKVTKEEMRDINH
CCCEEEEEECCCCCCCCCEEEEEHHHCCCCEECCCHHHHHHHHCCCCHHCCHHHHHHHHH
HMIDIVSPEEEFSVADFKNIGEKAIKEIIAKEKLPMIVGGTGLYINSLTCNVTFTESEKD
HEEEEECCHHHCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEEEEEEECCCCCC
DEYRTYLESLAEANGNNYVHEMLREIDEISYRDIHPNNRKRVIRALEVYKISGKPFSSYN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEECCCCCCCCC
AGNDFYKTDYHVFYYVLTMDREKLYDRINKRVDIMIENGLIDECIELKKLGYTSSMQSMQ
CCCCCCCCCEEEEEEEEECCHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHH
GIGYKEILYYLDKKISLYEAVNLIKQGSRNYAKRQLTWFRRDPRCTFLDKDVLSDKEILS
CCCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEECHHHCCCHHHHH
KIVDDITNN
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA