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The map label for this gene is spaK [H]

Identifier: 187934808

GI number: 187934808

Start: 2291625

End: 2292986

Strand: Reverse

Name: spaK [H]

Synonym: CLL_A2206

Alternate gene names: 187934808

Gene position: 2292986-2291625 (Counterclockwise)

Preceding gene: 187934010

Following gene: 187934906

Centisome position: 60.34

GC content: 24.67

Gene sequence:

>1362_bases
GTGGAAAGAATAAAGAAACAAAAAAGCATAGCTAAAATATTTGCTACGTATATAGCTACATTTTGTATAATTACAGTTCT
TCAAATTATAATAATCTCATTTTTATTTATTATAGGACTTAAAAATGGAATGATTTTACCAGCTAATTATTATGAACAAA
TGGTAGAGAAAAATAGAAACAATATTATGGTAACAGATGAAGTATCGGAATTTATACCCAAAGAATGCAATTATGTGGTA
TATGATTTAAATGGAAAAATCATTAGTAGCAATGTATCAGAAGAAAATGCATTAGGAATATGGAAAGTGATTGAAGAAGA
TAGAACTAGTTATGGTAATAATTATTATAAGATAATACCAAGAGGCAATGAAATTTGTGCTATAGGATATAAATTAACAG
CTCAGTTCTCTAATCCAATTTTACGTAAATATCTTCCAAGTGCTGAATGGTGTCCAGTAATTTTATTTATTATAACATTT
ATATTAGGAATAATAATTTTATCTAAATGTTTTAGTAAAAGAGTAGCTAAAGAAATGAAGATTTTAAAAAATATTACTGA
AAGCATAAAGGTAGAGAATCTAAATTTTGAGACAGAAGAATCAAGTATAACTGAAGTCAACGAAGTACTATCGGCTTTAG
ACAAAATGAGATTAGAATTAAATGTATCACTTAATAAACAGTGGAATTTAGAAAAATCAAGAAAAGATCAAATGGCAGCA
TTAGCACATGACATAAAAACACCATTAACTATAATTAAAGGTAATTCAGAACTACTTAGGGAATTAAATTTAAATTCTGA
TGCTATGGTATTTAATGAAAATATATTAAATGAAATTAAAAATATGGAGTATTATATAAAATCTTTAATAGAAATTATGA
AATCAGAAAAAGAAATGGTTTTTAAAAAAGAAGTGGTAAATTTAAAAAATTTCATAAGTGAAATAAAGGAAAAGTCAATT
TTACTTGGAAGGGAAAAAGAAATTAATTTTATAACTGAAATTAATAATCTTCCAGAGAGCATTAATGCAGATAAGATTGC
TTTAAAGCGTGCAATAATAAATTTAATTAACAATAGTATAGATTATTGTCCTATGAAAGGAAAAATATTATTTAAAGTTG
ATTTAAATGATAATTATATAAGTTTTAGCATAGAGGATGATGGCAGAGGTTTTAGTGAAGAAGAATTAAGTTTAGCAACA
AACCAGTTCTATCAAGGAGATAAAAGTAGAAATTCTAAAAATCATTATGGAATGGGACTTTATATAACTAAAAATATTAT
AAATAAACATGGTGGAAATCTTATATTAGAAAATTCAAAGGAAATAGGAGGAGCAAATGTTGTTATTAAGTTACCATTAT
AG

Upstream 100 bases:

>100_bases
GTGATACAACTGCTATTGTAGAACATATTAAAAATATAAGAAGTAAACTAAAAGTAAGAGGTATAAATACAATAGAAACT
GTTTGGGGGATTGGTTATAA

Downstream 100 bases:

>100_bases
AAAACTAATAAACTATTTTAAGACACTAAATGGTTATATAAAGTTAGATACTAGCAAAATTATTTTTTATAAAGACAATC
ACTTGATTTTAGTATTGTAA

Product: subtilin biosynthesis sensor protein SpaK

Products: NA

Alternate protein names: Subtilin biosynthesis sensor protein spaK [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MERIKKQKSIAKIFATYIATFCIITVLQIIIISFLFIIGLKNGMILPANYYEQMVEKNRNNIMVTDEVSEFIPKECNYVV
YDLNGKIISSNVSEENALGIWKVIEEDRTSYGNNYYKIIPRGNEICAIGYKLTAQFSNPILRKYLPSAEWCPVILFIITF
ILGIIILSKCFSKRVAKEMKILKNITESIKVENLNFETEESSITEVNEVLSALDKMRLELNVSLNKQWNLEKSRKDQMAA
LAHDIKTPLTIIKGNSELLRELNLNSDAMVFNENILNEIKNMEYYIKSLIEIMKSEKEMVFKKEVVNLKNFISEIKEKSI
LLGREKEINFITEINNLPESINADKIALKRAIINLINNSIDYCPMKGKILFKVDLNDNYISFSIEDDGRGFSEEELSLAT
NQFYQGDKSRNSKNHYGMGLYITKNIINKHGGNLILENSKEIGGANVVIKLPL

Sequences:

>Translated_453_residues
MERIKKQKSIAKIFATYIATFCIITVLQIIIISFLFIIGLKNGMILPANYYEQMVEKNRNNIMVTDEVSEFIPKECNYVV
YDLNGKIISSNVSEENALGIWKVIEEDRTSYGNNYYKIIPRGNEICAIGYKLTAQFSNPILRKYLPSAEWCPVILFIITF
ILGIIILSKCFSKRVAKEMKILKNITESIKVENLNFETEESSITEVNEVLSALDKMRLELNVSLNKQWNLEKSRKDQMAA
LAHDIKTPLTIIKGNSELLRELNLNSDAMVFNENILNEIKNMEYYIKSLIEIMKSEKEMVFKKEVVNLKNFISEIKEKSI
LLGREKEINFITEINNLPESINADKIALKRAIINLINNSIDYCPMKGKILFKVDLNDNYISFSIEDDGRGFSEEELSLAT
NQFYQGDKSRNSKNHYGMGLYITKNIINKHGGNLILENSKEIGGANVVIKLPL
>Mature_453_residues
MERIKKQKSIAKIFATYIATFCIITVLQIIIISFLFIIGLKNGMILPANYYEQMVEKNRNNIMVTDEVSEFIPKECNYVV
YDLNGKIISSNVSEENALGIWKVIEEDRTSYGNNYYKIIPRGNEICAIGYKLTAQFSNPILRKYLPSAEWCPVILFIITF
ILGIIILSKCFSKRVAKEMKILKNITESIKVENLNFETEESSITEVNEVLSALDKMRLELNVSLNKQWNLEKSRKDQMAA
LAHDIKTPLTIIKGNSELLRELNLNSDAMVFNENILNEIKNMEYYIKSLIEIMKSEKEMVFKKEVVNLKNFISEIKEKSI
LLGREKEINFITEINNLPESINADKIALKRAIINLINNSIDYCPMKGKILFKVDLNDNYISFSIEDDGRGFSEEELSLAT
NQFYQGDKSRNSKNHYGMGLYITKNIINKHGGNLILENSKEIGGANVVIKLPL

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaK may function as a membrane-associated protein kinase that phosphorylates spaR in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790861, Length=309, Percent_Identity=22.9773462783171, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1786600, Length=215, Percent_Identity=28.8372093023256, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790346, Length=283, Percent_Identity=25.0883392226148, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788549, Length=217, Percent_Identity=27.6497695852535, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789808, Length=256, Percent_Identity=28.125, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788393, Length=233, Percent_Identity=24.4635193133047, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788279, Length=294, Percent_Identity=27.5510204081633, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786912, Length=229, Percent_Identity=26.2008733624454, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786783, Length=220, Percent_Identity=24.0909090909091, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI48994928, Length=222, Percent_Identity=22.972972972973, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790551, Length=307, Percent_Identity=22.1498371335505, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI145693157, Length=226, Percent_Identity=26.1061946902655, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790436, Length=237, Percent_Identity=24.8945147679325, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87082128, Length=308, Percent_Identity=20.4545454545455, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR008358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51927; Mature: 51927

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIKKQKSIAKIFATYIATFCIITVLQIIIISFLFIIGLKNGMILPANYYEQMVEKNRN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHCCCC
NIMVTDEVSEFIPKECNYVVYDLNGKIISSNVSEENALGIWKVIEEDRTSYGNNYYKIIP
CEEEECCHHHCCCCCCCEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
RGNEICAIGYKLTAQFSNPILRKYLPSAEWCPVILFIITFILGIIILSKCFSKRVAKEMK
CCCCEEEEEEEEEEHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKNITESIKVENLNFETEESSITEVNEVLSALDKMRLELNVSLNKQWNLEKSRKDQMAA
HHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHH
LAHDIKTPLTIIKGNSELLRELNLNSDAMVFNENILNEIKNMEYYIKSLIEIMKSEKEMV
HHHHHCCCEEEEECCHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKKEVVNLKNFISEIKEKSILLGREKEINFITEINNLPESINADKIALKRAIINLINNSI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
DYCPMKGKILFKVDLNDNYISFSIEDDGRGFSEEELSLATNQFYQGDKSRNSKNHYGMGL
CCCCCCCEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEE
YITKNIINKHGGNLILENSKEIGGANVVIKLPL
EEEHHHHHCCCCCEEEECCCCCCCCEEEEEECC
>Mature Secondary Structure
MERIKKQKSIAKIFATYIATFCIITVLQIIIISFLFIIGLKNGMILPANYYEQMVEKNRN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHCCCC
NIMVTDEVSEFIPKECNYVVYDLNGKIISSNVSEENALGIWKVIEEDRTSYGNNYYKIIP
CEEEECCHHHCCCCCCCEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
RGNEICAIGYKLTAQFSNPILRKYLPSAEWCPVILFIITFILGIIILSKCFSKRVAKEMK
CCCCEEEEEEEEEEHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKNITESIKVENLNFETEESSITEVNEVLSALDKMRLELNVSLNKQWNLEKSRKDQMAA
HHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHH
LAHDIKTPLTIIKGNSELLRELNLNSDAMVFNENILNEIKNMEYYIKSLIEIMKSEKEMV
HHHHHCCCEEEEECCHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKKEVVNLKNFISEIKEKSILLGREKEINFITEINNLPESINADKIALKRAIINLINNSI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
DYCPMKGKILFKVDLNDNYISFSIEDDGRGFSEEELSLATNQFYQGDKSRNSKNHYGMGL
CCCCCCCEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEE
YITKNIINKHGGNLILENSKEIGGANVVIKLPL
EEEHHHHHCCCCCEEEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8439156; 8085823 [H]