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The map label for this gene is mccA [H]

Identifier: 187934784

GI number: 187934784

Start: 3491314

End: 3492222

Strand: Reverse

Name: mccA [H]

Synonym: CLL_A3318

Alternate gene names: 187934784

Gene position: 3492222-3491314 (Counterclockwise)

Preceding gene: 187932450

Following gene: 187935767

Centisome position: 91.89

GC content: 31.79

Gene sequence:

>909_bases
GTGAAATATATAGATGATATTAGAAGTCATATAGGAAATACACCTATTATGAAACTAAATAATATGGGAATAAAAGCTGG
TGTAAATGTATTTGCTAAATTAGAATTTTTAAATCCTGGTGGAAGTGTTAAAGATAGAATAGGTATATACATGATAGAAG
ATGCTGAGAATAAGGGATTACTTAGAGCAGGCTATACAATAATAGAAGCTACAGCAGGTAACACAGGTCTTGGTATAGCA
TTAGCAGCCATAAATAAAGGGTATAGAGTAATATTTGTTGTACCAGAAAAATTTTCTGTAGAAAAGCAGACGTTAATGAA
AGCCCTTGGGGCAGAAATAGTAAATACTCCTAGAGAAGACGGAATGTTAGGAGCTGTTGAAAAGGCTAATGAACTTTTAA
AAACAATAAAAAATTCTATAACTTTAAAACAATTTGAGAATAAATCTAATCCATTAGCCCATTATGAAACTACAGGTCCT
GAAATATATAAAGATTTAGATGGAAAAATAGACTATTTCCTTTGTGGTGCAGGTAGTGGTGGAACGTATACTGGTATATT
AAAGTATTTAAAAGAAAAGAATTCTAATATAAAAGGAGTTTTAGTAGATCCAGAGGGGTCAACTATGGGAGGCGGAGAAG
AAGCTTGTTATGATATAGAAGGGATAGGAAATAACTTTATTCCTAAAACTATGGACATGACTTATGTTGATGATGTTATA
AAGGTTAATGATGAAGAAGGATTTCATATTGTAAAACAACTTGCTTTAAAAGAAGGATTAATTGTTGGGACATCTTCTGG
AGCAGCAGTAGCAGCAGCCCTTAAACTAGCAAGTAAAATAGATAGTGGGAATATCGTAACATTATTGCCAGATAGAGGGG
AAAGATATTTTAGCAAAAATATATATTAA

Upstream 100 bases:

>100_bases
ATACCAGCAACAAATAAATTTCAATGTGGGAATTATAGAGATCATTCATTATTTGCAGGAAAAGAATATATTAAATTAAT
ATTAAAAGAGGGGATTAGTA

Downstream 100 bases:

>100_bases
TCATGAAAAGTTTAATTACTCAACGGAACTAATGATTAATTGAAAAATCTAAGGTCAAATAACAATAAAATTGCACAAAA
ATAATTAAAAGGTACACTAT

Product: cysteine synthase family protein

Products: L-cysteine; acetate

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKYIDDIRSHIGNTPIMKLNNMGIKAGVNVFAKLEFLNPGGSVKDRIGIYMIEDAENKGLLRAGYTIIEATAGNTGLGIA
LAAINKGYRVIFVVPEKFSVEKQTLMKALGAEIVNTPREDGMLGAVEKANELLKTIKNSITLKQFENKSNPLAHYETTGP
EIYKDLDGKIDYFLCGAGSGGTYTGILKYLKEKNSNIKGVLVDPEGSTMGGGEEACYDIEGIGNNFIPKTMDMTYVDDVI
KVNDEEGFHIVKQLALKEGLIVGTSSGAAVAAALKLASKIDSGNIVTLLPDRGERYFSKNIY

Sequences:

>Translated_302_residues
MKYIDDIRSHIGNTPIMKLNNMGIKAGVNVFAKLEFLNPGGSVKDRIGIYMIEDAENKGLLRAGYTIIEATAGNTGLGIA
LAAINKGYRVIFVVPEKFSVEKQTLMKALGAEIVNTPREDGMLGAVEKANELLKTIKNSITLKQFENKSNPLAHYETTGP
EIYKDLDGKIDYFLCGAGSGGTYTGILKYLKEKNSNIKGVLVDPEGSTMGGGEEACYDIEGIGNNFIPKTMDMTYVDDVI
KVNDEEGFHIVKQLALKEGLIVGTSSGAAVAAALKLASKIDSGNIVTLLPDRGERYFSKNIY
>Mature_302_residues
MKYIDDIRSHIGNTPIMKLNNMGIKAGVNVFAKLEFLNPGGSVKDRIGIYMIEDAENKGLLRAGYTIIEATAGNTGLGIA
LAAINKGYRVIFVVPEKFSVEKQTLMKALGAEIVNTPREDGMLGAVEKANELLKTIKNSITLKQFENKSNPLAHYETTGP
EIYKDLDGKIDYFLCGAGSGGTYTGILKYLKEKNSNIKGVLVDPEGSTMGGGEEACYDIEGIGNNFIPKTMDMTYVDDVI
KVNDEEGFHIVKQLALKEGLIVGTSSGAAVAAALKLASKIDSGNIVTLLPDRGERYFSKNIY

Specific function: Catalyzes the conversion of O-acetylserine and homocysteine to cystathionine [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=310, Percent_Identity=48.0645161290323, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI295821200, Length=310, Percent_Identity=48.0645161290323, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI4557415, Length=310, Percent_Identity=48.0645161290323, Blast_Score=270, Evalue=1e-72,
Organism=Escherichia coli, GI1788754, Length=313, Percent_Identity=41.85303514377, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI2367138, Length=300, Percent_Identity=37.6666666666667, Blast_Score=218, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17535051, Length=296, Percent_Identity=41.8918918918919, Blast_Score=238, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI115535073, Length=296, Percent_Identity=44.2567567567568, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI17562970, Length=305, Percent_Identity=39.0163934426229, Blast_Score=220, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI32566674, Length=300, Percent_Identity=39, Blast_Score=214, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17534315, Length=313, Percent_Identity=41.85303514377, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI25147552, Length=311, Percent_Identity=40.5144694533762, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17561720, Length=298, Percent_Identity=40.2684563758389, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI32566672, Length=212, Percent_Identity=36.3207547169811, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17561716, Length=118, Percent_Identity=33.8983050847458, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321594, Length=310, Percent_Identity=40, Blast_Score=205, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6321449, Length=321, Percent_Identity=36.7601246105919, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24643623, Length=301, Percent_Identity=45.514950166113, Blast_Score=233, Evalue=8e-62,
Organism=Drosophila melanogaster, GI20129101, Length=301, Percent_Identity=45.514950166113, Blast_Score=233, Evalue=8e-62,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 2.5.1.47

Molecular weight: Translated: 32658; Mature: 32658

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIDDIRSHIGNTPIMKLNNMGIKAGVNVFAKLEFLNPGGSVKDRIGIYMIEDAENKGL
CCHHHHHHHHHCCCCEEEECCCCCHHCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCCE
LRAGYTIIEATAGNTGLGIALAAINKGYRVIFVVPEKFSVEKQTLMKALGAEIVNTPRED
EEECCEEEEECCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCC
GMLGAVEKANELLKTIKNSITLKQFENKSNPLAHYETTGPEIYKDLDGKIDYFLCGAGSG
CCCHHHHHHHHHHHHHHHHEEHHHHCCCCCCCEEEECCCHHHHHHCCCCEEEEEEECCCC
GTYTGILKYLKEKNSNIKGVLVDPEGSTMGGGEEACYDIEGIGNNFIPKTMDMTYVDDVI
CHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHEE
KVNDEEGFHIVKQLALKEGLIVGTSSGAAVAAALKLASKIDSGNIVTLLPDRGERYFSKN
EECCCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCC
IY
CC
>Mature Secondary Structure
MKYIDDIRSHIGNTPIMKLNNMGIKAGVNVFAKLEFLNPGGSVKDRIGIYMIEDAENKGL
CCHHHHHHHHHCCCCEEEECCCCCHHCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCCE
LRAGYTIIEATAGNTGLGIALAAINKGYRVIFVVPEKFSVEKQTLMKALGAEIVNTPRED
EEECCEEEEECCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCC
GMLGAVEKANELLKTIKNSITLKQFENKSNPLAHYETTGPEIYKDLDGKIDYFLCGAGSG
CCCHHHHHHHHHHHHHHHHEEHHHHCCCCCCCEEEECCCHHHHHHCCCCEEEEEEECCCC
GTYTGILKYLKEKNSNIKGVLVDPEGSTMGGGEEACYDIEGIGNNFIPKTMDMTYVDDVI
CHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHEE
KVNDEEGFHIVKQLALKEGLIVGTSSGAAVAAALKLASKIDSGNIVTLLPDRGERYFSKN
EECCCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCC
IY
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 44000 [C]

Specific activity: 1100

Km value (mM): 0.7 {L-Ser}} 3.6 {S2-}} 0.1 {S2-}} 0.12 {acetyl-CoA}} 20 {O-acetyl-L-Ser}} 7.1 {O-acetyl-L-Ser}} 5 {O-acetyl-L-Ser}} [C]

Substrates: O3-acetyl-L-serine; hydrogen sulfide

Specific reaction: O3-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate

General reaction: Elimination; C-O bond cleavage [C]

Inhibitor: acetyl-CoA; D-cycloserine; L-cysteine; NH2OH; semicarbazide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]