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The map label for this gene is rumA [C]

Identifier: 187934647

GI number: 187934647

Start: 3502389

End: 3503756

Strand: Reverse

Name: rumA [C]

Synonym: CLL_A3330

Alternate gene names: 187934647

Gene position: 3503756-3502389 (Counterclockwise)

Preceding gene: 187933936

Following gene: 187933795

Centisome position: 92.2

GC content: 27.19

Gene sequence:

>1368_bases
TTGATAGAAAAAAATAAAGAATACATTTTAGATATATTATCACAAGGATATGAAGGTGAGGGAGTAGCCAAAGTAGAAGG
ATATCCTGTATTTATTCAAGGAGCCTTACAAGGTGAAAAAGTTAAAACAAAAATTATAAAAGTTAAGAAAAATTATGCAT
ATGGTAAGATAGAAAAAATAATTAATACATCAGAAGATAGAGAAGAACCTAAATGTTCAAATTATAAAAGATGTGGTGGA
TGTTCAATTCAACACATGACTTATAAAAAACAATTAGATTTTAAATGGGAAAGAGTAAAGGACTGTATAGAAAAGATTGG
TGGATTATCTAAGGAGATAATAAATTATCCTATGGGAATGGAATCTAATCCGTATAGATATAGAAATAAGGTTCAATTAC
CTATCGGAAAAGTAAATAATGAAATTGTAATAGGTTTTTATGCACCAAGAAGTCATGACATAATAGATTTAGATTATTGT
TTAATTCAAGATAAAGTTGCAGATGATGTAGCAAATCTTACAAAACAATGGATAAAAATAAATAATATTGAACCAGCAAC
TATAGATGGAGAGTTTAATTCTAACGGTATTTTAAAGCATTTAATGATAAGAAGAGGATTTAAAACAGATGAAGTAATGG
TTGTTCTTGTTGCTACAACTTCAAATATTCCTAAATTAGATGAATTTAAACATATAATATTAGAAAATATAAAAGGGATA
AAAAGTATAGTATTAAATATAAACCCTAAAAATACAAATGTTATTTTAGGTGAAGAATGTATAACATTATATGGTGAAGA
TACTATTAAAGATTACATAGGGGAATTTAAATTTAATATATCTCCATTATCATTTTTTCAGGTTAACCCAATTCAAACTG
AGATATTGTATAATAAAGCATTAGAATATGCAGGGTTAACAGGTAATGAAACTGTTTTTGATGCATATTGTGGAACTGGA
ACAATAACTTTATTTTTATCTCAAAAAGCAAAAAAAGTGTACGGTGTTGAGATAATAGAACCTGCAATTATTAACGCTAA
AGAAAATGCTAAGCTTAATAATGTGGATAATGCAGAATTTTTTGTTGGTAAATCTGAGGAAGTAATTCCAAATCTTATAA
GTGAAGGATTAAAAGCTGATATAATAGTTGTGGATCCACCAAGAAAGGGTTGCGATATTAAATTATTAGAGGCAATCGGA
GATGTTAAACCAGAAAGAGTAGTTTATGTTTCATGTGATCCAAGTACATTATCTAGAGATTTAAAAATCTTAGATGAAAA
AGGATACAAGGTTGTTGAAATTCAGCCGGTAGATATGTTTCCACAAACTTCTCATATTGAAAATGTAGTAAAATTAATAA
AAAAATAG

Upstream 100 bases:

>100_bases
AGTGAATTTATAACCTTAAATGTTATTAATTAAACTATTATATAAAACTTATAATGTGATATAATTTATAGAAGTTATAA
TTAAAAAGGTGGATTTTAAA

Downstream 100 bases:

>100_bases
GCATTAAATGCAGTAATAGAAATCCTAATTAGAAATTTATCTAGTTGGGATTTTTGTTTTATACACTTTAGAATATTAAA
CATTTGTTAAGTATAAATTT

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MIEKNKEYILDILSQGYEGEGVAKVEGYPVFIQGALQGEKVKTKIIKVKKNYAYGKIEKIINTSEDREEPKCSNYKRCGG
CSIQHMTYKKQLDFKWERVKDCIEKIGGLSKEIINYPMGMESNPYRYRNKVQLPIGKVNNEIVIGFYAPRSHDIIDLDYC
LIQDKVADDVANLTKQWIKINNIEPATIDGEFNSNGILKHLMIRRGFKTDEVMVVLVATTSNIPKLDEFKHIILENIKGI
KSIVLNINPKNTNVILGEECITLYGEDTIKDYIGEFKFNISPLSFFQVNPIQTEILYNKALEYAGLTGNETVFDAYCGTG
TITLFLSQKAKKVYGVEIIEPAIINAKENAKLNNVDNAEFFVGKSEEVIPNLISEGLKADIIVVDPPRKGCDIKLLEAIG
DVKPERVVYVSCDPSTLSRDLKILDEKGYKVVEIQPVDMFPQTSHIENVVKLIKK

Sequences:

>Translated_455_residues
MIEKNKEYILDILSQGYEGEGVAKVEGYPVFIQGALQGEKVKTKIIKVKKNYAYGKIEKIINTSEDREEPKCSNYKRCGG
CSIQHMTYKKQLDFKWERVKDCIEKIGGLSKEIINYPMGMESNPYRYRNKVQLPIGKVNNEIVIGFYAPRSHDIIDLDYC
LIQDKVADDVANLTKQWIKINNIEPATIDGEFNSNGILKHLMIRRGFKTDEVMVVLVATTSNIPKLDEFKHIILENIKGI
KSIVLNINPKNTNVILGEECITLYGEDTIKDYIGEFKFNISPLSFFQVNPIQTEILYNKALEYAGLTGNETVFDAYCGTG
TITLFLSQKAKKVYGVEIIEPAIINAKENAKLNNVDNAEFFVGKSEEVIPNLISEGLKADIIVVDPPRKGCDIKLLEAIG
DVKPERVVYVSCDPSTLSRDLKILDEKGYKVVEIQPVDMFPQTSHIENVVKLIKK
>Mature_455_residues
MIEKNKEYILDILSQGYEGEGVAKVEGYPVFIQGALQGEKVKTKIIKVKKNYAYGKIEKIINTSEDREEPKCSNYKRCGG
CSIQHMTYKKQLDFKWERVKDCIEKIGGLSKEIINYPMGMESNPYRYRNKVQLPIGKVNNEIVIGFYAPRSHDIIDLDYC
LIQDKVADDVANLTKQWIKINNIEPATIDGEFNSNGILKHLMIRRGFKTDEVMVVLVATTSNIPKLDEFKHIILENIKGI
KSIVLNINPKNTNVILGEECITLYGEDTIKDYIGEFKFNISPLSFFQVNPIQTEILYNKALEYAGLTGNETVFDAYCGTG
TITLFLSQKAKKVYGVEIIEPAIINAKENAKLNNVDNAEFFVGKSEEVIPNLISEGLKADIIVVDPPRKGCDIKLLEAIG
DVKPERVVYVSCDPSTLSRDLKILDEKGYKVVEIQPVDMFPQTSHIENVVKLIKK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=206, Percent_Identity=36.4077669902913, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI51173878, Length=206, Percent_Identity=36.4077669902913, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI269784678, Length=400, Percent_Identity=26, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI269784680, Length=387, Percent_Identity=25.5813953488372, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI269784676, Length=387, Percent_Identity=25.5813953488372, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI20149688, Length=387, Percent_Identity=25.5813953488372, Blast_Score=116, Evalue=5e-26,
Organism=Escherichia coli, GI1789148, Length=432, Percent_Identity=28.0092592592593, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1787083, Length=393, Percent_Identity=22.9007633587786, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1790403, Length=185, Percent_Identity=30.8108108108108, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71997438, Length=163, Percent_Identity=33.1288343558282, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6322909, Length=167, Percent_Identity=34.7305389221557, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24666579, Length=474, Percent_Identity=29.1139240506329, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 51420; Mature: 51420

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKNKEYILDILSQGYEGEGVAKVEGYPVFIQGALQGEKVKTKIIKVKKNYAYGKIEKI
CCCCCHHHHHHHHHCCCCCCCEEEECCEEEEEEECCCCCCEEEEEEEEECCCCHHHHHHH
INTSEDREEPKCSNYKRCGGCSIQHMTYKKQLDFKWERVKDCIEKIGGLSKEIINYPMGM
HCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHCCCCHHHHHCCCCC
ESNPYRYRNKVQLPIGKVNNEIVIGFYAPRSHDIIDLDYCLIQDKVADDVANLTKQWIKI
CCCCCEECCEEEEEECCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHEEE
NNIEPATIDGEFNSNGILKHLMIRRGFKTDEVMVVLVATTSNIPKLDEFKHIILENIKGI
CCCCCEEECCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCC
KSIVLNINPKNTNVILGEECITLYGEDTIKDYIGEFKFNISPLSFFQVNPIQTEILYNKA
EEEEEEECCCCCEEEECCCEEEECCCHHHHHHHHHEEECCCCEEEEEECCCHHHHHHHHH
LEYAGLTGNETVFDAYCGTGTITLFLSQKAKKVYGVEIIEPAIINAKENAKLNNVDNAEF
HHHCCCCCCCEEEEEECCCCEEEEEEECCCCCEECEEEECCEEECCCCCCCCCCCCCCEE
FVGKSEEVIPNLISEGLKADIIVVDPPRKGCDIKLLEAIGDVKPERVVYVSCDPSTLSRD
EECCCHHHHHHHHHCCCCCCEEEECCCCCCCCEEHHHHHCCCCCCEEEEEECCHHHHHHH
LKILDEKGYKVVEIQPVDMFPQTSHIENVVKLIKK
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIEKNKEYILDILSQGYEGEGVAKVEGYPVFIQGALQGEKVKTKIIKVKKNYAYGKIEKI
CCCCCHHHHHHHHHCCCCCCCEEEECCEEEEEEECCCCCCEEEEEEEEECCCCHHHHHHH
INTSEDREEPKCSNYKRCGGCSIQHMTYKKQLDFKWERVKDCIEKIGGLSKEIINYPMGM
HCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHCCCCHHHHHCCCCC
ESNPYRYRNKVQLPIGKVNNEIVIGFYAPRSHDIIDLDYCLIQDKVADDVANLTKQWIKI
CCCCCEECCEEEEEECCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHEEE
NNIEPATIDGEFNSNGILKHLMIRRGFKTDEVMVVLVATTSNIPKLDEFKHIILENIKGI
CCCCCEEECCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCC
KSIVLNINPKNTNVILGEECITLYGEDTIKDYIGEFKFNISPLSFFQVNPIQTEILYNKA
EEEEEEECCCCCEEEECCCEEEECCCHHHHHHHHHEEECCCCEEEEEECCCHHHHHHHHH
LEYAGLTGNETVFDAYCGTGTITLFLSQKAKKVYGVEIIEPAIINAKENAKLNNVDNAEF
HHHCCCCCCCEEEEEECCCCEEEEEEECCCCCEECEEEECCEEECCCCCCCCCCCCCCEE
FVGKSEEVIPNLISEGLKADIIVVDPPRKGCDIKLLEAIGDVKPERVVYVSCDPSTLSRD
EECCCHHHHHHHHHCCCCCCEEEECCCCCCCCEEHHHHHCCCCCCEEEEEECCHHHHHHH
LKILDEKGYKVVEIQPVDMFPQTSHIENVVKLIKK
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]