Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 187932490

GI number: 187932490

Start: 2271628

End: 2272077

Strand: Reverse

Name: def [H]

Synonym: CLL_A2185

Alternate gene names: 187932490

Gene position: 2272077-2271628 (Counterclockwise)

Preceding gene: 187932496

Following gene: 187935608

Centisome position: 59.79

GC content: 30.67

Gene sequence:

>450_bases
ATGGCATTAAGACAAATTAGGCTTTATGATGATCCTATTCTTAGGAAAAAAAGCAAAGAAGTTGAAGTCGTAGATGATAA
GATAAGAGATATATTAAATGATATGGCAGAGACTATGTATAATACGGAAAATGGTGCAGGTTTGGCAGCACCACAAGTAG
GCATGTTAAAAAGATTAGTTGTAATAGACATGTGGCAGGGGCTTATCAAATTAATTAATCCTAAAATAATAAAGAAAGAA
GGAAGTCAAGAAGTTATAGAGGGATGTTTGAGTAATCCTAATGTATTTGGAAAACTAAAAAGACCATATAAAGTAATAAT
AGAAGCTCTAAATGAAAATGGAGAAGAAATTCAATTAACTGGCACTGGGGATTTAGCAAAATGTTTCTGTCATGAAATTG
ATCATTTAGACGGAATTTTATTTACTGATTTAGTTACAGAATATATCTAA

Upstream 100 bases:

>100_bases
TGATATTTCAAAAGTATTCAATTGTTCAATAGAAGAAGTTTTTATTTTTGAGGAAAGTAAAAGAAAATCAAGAGCAGATC
AAAGTAGAGGGGTAGTTTAA

Downstream 100 bases:

>100_bases
TAAATATTAAAATATCGCAAATTCATTTCTTCTGATATGCTCCCTTTAAGGTAGACAGATTAAAAAAATAAAAATCTGTC
TACCTTAAAGGGAGTATTTT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 149; Mature: 148

Protein sequence:

>149_residues
MALRQIRLYDDPILRKKSKEVEVVDDKIRDILNDMAETMYNTENGAGLAAPQVGMLKRLVVIDMWQGLIKLINPKIIKKE
GSQEVIEGCLSNPNVFGKLKRPYKVIIEALNENGEEIQLTGTGDLAKCFCHEIDHLDGILFTDLVTEYI

Sequences:

>Translated_149_residues
MALRQIRLYDDPILRKKSKEVEVVDDKIRDILNDMAETMYNTENGAGLAAPQVGMLKRLVVIDMWQGLIKLINPKIIKKE
GSQEVIEGCLSNPNVFGKLKRPYKVIIEALNENGEEIQLTGTGDLAKCFCHEIDHLDGILFTDLVTEYI
>Mature_148_residues
ALRQIRLYDDPILRKKSKEVEVVDDKIRDILNDMAETMYNTENGAGLAAPQVGMLKRLVVIDMWQGLIKLINPKIIKKEG
SQEVIEGCLSNPNVFGKLKRPYKVIIEALNENGEEIQLTGTGDLAKCFCHEIDHLDGILFTDLVTEYI

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=149, Percent_Identity=42.2818791946309, Blast_Score=103, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 16844; Mature: 16713

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRQIRLYDDPILRKKSKEVEVVDDKIRDILNDMAETMYNTENGAGLAAPQVGMLKRLV
CCCCCEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
VIDMWQGLIKLINPKIIKKEGSQEVIEGCLSNPNVFGKLKRPYKVIIEALNENGEEIQLT
HHHHHHHHHHHHCCHHHHCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCEEEEE
GTGDLAKCFCHEIDHLDGILFTDLVTEYI
CCCHHHHHHHHHHHHHCHHHHHHHHHHHC
>Mature Secondary Structure 
ALRQIRLYDDPILRKKSKEVEVVDDKIRDILNDMAETMYNTENGAGLAAPQVGMLKRLV
CCCCEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
VIDMWQGLIKLINPKIIKKEGSQEVIEGCLSNPNVFGKLKRPYKVIIEALNENGEEIQLT
HHHHHHHHHHHHCCHHHHCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCEEEEE
GTGDLAKCFCHEIDHLDGILFTDLVTEYI
CCCHHHHHHHHHHHHHCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]