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The map label for this gene is lpdA [H]

Identifier: 187932434

GI number: 187932434

Start: 3570335

End: 3572035

Strand: Reverse

Name: lpdA [H]

Synonym: CLL_A3389

Alternate gene names: 187932434

Gene position: 3572035-3570335 (Counterclockwise)

Preceding gene: 187935158

Following gene: 187933780

Centisome position: 93.99

GC content: 28.92

Gene sequence:

>1701_bases
ATGGATATAAAATTAGAAAAATTATCTGGACATAATAAGTCTGCTAAAGTAGGAAAAATTAGCGTGATTGAAGGAGAGCA
AGTAAAAAAAGGGCATGTATTGATGCAATTAGAGACAAGCAAAGGAAATACACCTTTTAAAGCAACTTTTGATTTTAAAA
TAGATAAAATAAAAGTGTCTGAAGGTGATGAAGTTGAAATTGGACAAATATTATTTTTAGCGACTGGAGAAAATATAAAT
GTTAACAGTAATACTCCTAAGGTAGATTATTTTGGCTCTATGCTTAAGGGGAAGAAAGAAAACGTTACAGCTGATTTAAC
GATAATAGGGGGAGGTCCAGGAGGATACGTTGCAGCTATTTATGCAGCTAAAAAGGGATTAAATACTGTTATTATCGAGA
AAGAAAATCTTGGAGGAACTTGTTTAAATGTAGGTTGTATTCCAACAAAAGCATTTGTAAAATCATCTGAGGTATTTCAT
AATGCATTAAATAGTGAGGAATTTGGATTCACTGCTGACAATTTACAAGTTGATATGAAAAAAGTAGTAAAAAGAAAAGA
TGATGTTAAAGGAAGACTTGTTAATGGAATAGAGTATCTTCTAGAAGCAAATGGTGTAAGGCTTATAAAAGGAGAAGCTT
CTTTTTTAGATAATAAAAATATTATTGTTAAAAGGGGAAAAGATGAGTATACAATAGAGTCTAAAGATATAATTATAGCA
ACTGGATCTAAAATTTCAAAGATTGATATTAAAGGAATTGATATGCCATTTGTTTTAAATAGTACTACTGCATTAAACAA
TGAAAAATTACCTGAATCTATAACAATAATTGGTGGTGGAGTTATAGGGATGGAATTTGCATTTATTTATTCTAACTTTG
GAGTAAAGGTAAATGTAGTTGAATATGCAGATAGATTATTGCTAATGATGGATAAAGATATTTCTGAAGAGATAAAAGAC
ATTGCAACTGATAAAGGAATTAATGTCTATACAAGCTCAAAAGTTACAGAGATTAAAAAATCTGAAGATGGCGATGCTAT
CGTATCTTTTGAAAAAGATGGAGAAGAAAAATTATTAGTATCAGAAAATGTACTTGTAGCAATAGGAAGAGAGCCTAATA
TTGATGGATTAAATATTGAAAATATAAACCTTGAATTAAATGATAATAGAAGAGGAATTAAAGTTAATTCGAAGTTAGAA
ACAAATGTTGAAGGTATTTATGCTATAGGAGATGTTAATAACATAATGCAATTAGCACATGTTGCTTCACATCAAGGAAT
GATAGCTGTTGATAATATATTAGGACAAAATAAAGAAATGAGCTATGATCATGTACCAAATGTAATTTTCACAGTACCAG
AAATAGCATCAGTTGGAATGAATGAAGATAAGTGTTTAAAAGAAGAACTAAATATTAAGATAAGTAAATTCCCTTATTCA
GCTAATGGTAAGGCTTTAACTATGGGAGAAGAAAAAGGATTTATAAAAATAATTAAAGATATTAATAATAATAAAATAGT
TGGAGCATCTATTATTGGAGCAGATGCATCTTCATTAATTAGCACATTAACTTTAATTATTAAGAATAATATCAGTGAAG
AGAAAATTTGTGAAACTATCTTCGCACATCCAACTACAGGTGAAGTAATACATGAAAGTTTCCTAGGATTATCTATTGGA
GCTATACACAATCATGAATAA

Upstream 100 bases:

>100_bases
TTTAAATAGTTATTAATTATATATAAATAAAAATATAGGGGTTGTTCATAACATCTAGTTGATTTTATGACAACCTTTTT
ACATAGAAGGAGAATTTAAG

Downstream 100 bases:

>100_bases
AGTTATCATTTCAAAAGAGGTAGATCCAGCTTATAATTTAGCTTTAGAAGAAGAACTACTTAGAAATTTGAAGGATAATG
AAAATATACTATATCTTTGG

Product: dihydrolipoyl dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; S complex, 50 kDa subunit [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MDIKLEKLSGHNKSAKVGKISVIEGEQVKKGHVLMQLETSKGNTPFKATFDFKIDKIKVSEGDEVEIGQILFLATGENIN
VNSNTPKVDYFGSMLKGKKENVTADLTIIGGGPGGYVAAIYAAKKGLNTVIIEKENLGGTCLNVGCIPTKAFVKSSEVFH
NALNSEEFGFTADNLQVDMKKVVKRKDDVKGRLVNGIEYLLEANGVRLIKGEASFLDNKNIIVKRGKDEYTIESKDIIIA
TGSKISKIDIKGIDMPFVLNSTTALNNEKLPESITIIGGGVIGMEFAFIYSNFGVKVNVVEYADRLLLMMDKDISEEIKD
IATDKGINVYTSSKVTEIKKSEDGDAIVSFEKDGEEKLLVSENVLVAIGREPNIDGLNIENINLELNDNRRGIKVNSKLE
TNVEGIYAIGDVNNIMQLAHVASHQGMIAVDNILGQNKEMSYDHVPNVIFTVPEIASVGMNEDKCLKEELNIKISKFPYS
ANGKALTMGEEKGFIKIIKDINNNKIVGASIIGADASSLISTLTLIIKNNISEEKICETIFAHPTTGEVIHESFLGLSIG
AIHNHE

Sequences:

>Translated_566_residues
MDIKLEKLSGHNKSAKVGKISVIEGEQVKKGHVLMQLETSKGNTPFKATFDFKIDKIKVSEGDEVEIGQILFLATGENIN
VNSNTPKVDYFGSMLKGKKENVTADLTIIGGGPGGYVAAIYAAKKGLNTVIIEKENLGGTCLNVGCIPTKAFVKSSEVFH
NALNSEEFGFTADNLQVDMKKVVKRKDDVKGRLVNGIEYLLEANGVRLIKGEASFLDNKNIIVKRGKDEYTIESKDIIIA
TGSKISKIDIKGIDMPFVLNSTTALNNEKLPESITIIGGGVIGMEFAFIYSNFGVKVNVVEYADRLLLMMDKDISEEIKD
IATDKGINVYTSSKVTEIKKSEDGDAIVSFEKDGEEKLLVSENVLVAIGREPNIDGLNIENINLELNDNRRGIKVNSKLE
TNVEGIYAIGDVNNIMQLAHVASHQGMIAVDNILGQNKEMSYDHVPNVIFTVPEIASVGMNEDKCLKEELNIKISKFPYS
ANGKALTMGEEKGFIKIIKDINNNKIVGASIIGADASSLISTLTLIIKNNISEEKICETIFAHPTTGEVIHESFLGLSIG
AIHNHE
>Mature_566_residues
MDIKLEKLSGHNKSAKVGKISVIEGEQVKKGHVLMQLETSKGNTPFKATFDFKIDKIKVSEGDEVEIGQILFLATGENIN
VNSNTPKVDYFGSMLKGKKENVTADLTIIGGGPGGYVAAIYAAKKGLNTVIIEKENLGGTCLNVGCIPTKAFVKSSEVFH
NALNSEEFGFTADNLQVDMKKVVKRKDDVKGRLVNGIEYLLEANGVRLIKGEASFLDNKNIIVKRGKDEYTIESKDIIIA
TGSKISKIDIKGIDMPFVLNSTTALNNEKLPESITIIGGGVIGMEFAFIYSNFGVKVNVVEYADRLLLMMDKDISEEIKD
IATDKGINVYTSSKVTEIKKSEDGDAIVSFEKDGEEKLLVSENVLVAIGREPNIDGLNIENINLELNDNRRGIKVNSKLE
TNVEGIYAIGDVNNIMQLAHVASHQGMIAVDNILGQNKEMSYDHVPNVIFTVPEIASVGMNEDKCLKEELNIKISKFPYS
ANGKALTMGEEKGFIKIIKDINNNKIVGASIIGADASSLISTLTLIIKNNISEEKICETIFAHPTTGEVIHESFLGLSIG
AIHNHE

Specific function: Catalyzes the oxidation of dihydrolipoamide to lipoamide [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=470, Percent_Identity=36.3829787234043, Blast_Score=284, Evalue=2e-76,
Organism=Homo sapiens, GI50301238, Length=447, Percent_Identity=27.0693512304251, Blast_Score=150, Evalue=5e-36,
Organism=Homo sapiens, GI291045266, Length=430, Percent_Identity=25.5813953488372, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI22035672, Length=428, Percent_Identity=25, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI33519430, Length=439, Percent_Identity=24.6013667425968, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI33519428, Length=439, Percent_Identity=24.6013667425968, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI33519426, Length=439, Percent_Identity=24.6013667425968, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI148277071, Length=444, Percent_Identity=24.5495495495495, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI148277065, Length=439, Percent_Identity=24.6013667425968, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI291045268, Length=425, Percent_Identity=23.7647058823529, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI21389617, Length=263, Percent_Identity=24.7148288973384, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI226437568, Length=263, Percent_Identity=24.7148288973384, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI65787454, Length=263, Percent_Identity=24.7148288973384, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1786307, Length=458, Percent_Identity=32.3144104803493, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI87081717, Length=453, Percent_Identity=30.6843267108168, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI87082354, Length=458, Percent_Identity=28.82096069869, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1789915, Length=432, Percent_Identity=27.3148148148148, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1789065, Length=273, Percent_Identity=22.3443223443223, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=489, Percent_Identity=34.7648261758691, Blast_Score=288, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI71983429, Length=436, Percent_Identity=26.3761467889908, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71983419, Length=436, Percent_Identity=26.3761467889908, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17557007, Length=469, Percent_Identity=23.2409381663113, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71982272, Length=434, Percent_Identity=24.4239631336406, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=486, Percent_Identity=35.3909465020576, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6325240, Length=465, Percent_Identity=28.1720430107527, Blast_Score=179, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6325166, Length=446, Percent_Identity=28.2511210762332, Blast_Score=160, Evalue=7e-40,
Organism=Drosophila melanogaster, GI21358499, Length=465, Percent_Identity=36.1290322580645, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24640553, Length=465, Percent_Identity=26.4516129032258, Blast_Score=145, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24640549, Length=465, Percent_Identity=26.4516129032258, Blast_Score=145, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24640551, Length=465, Percent_Identity=26.4516129032258, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17737741, Length=494, Percent_Identity=25.9109311740891, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 61671; Mature: 61671

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKLEKLSGHNKSAKVGKISVIEGEQVKKGHVLMQLETSKGNTPFKATFDFKIDKIKVS
CCEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCEEEEEEEEEEEEEEC
EGDEVEIGQILFLATGENINVNSNTPKVDYFGSMLKGKKENVTADLTIIGGGPGGYVAAI
CCCCEEECEEEEEEECCEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEE
YAAKKGLNTVIIEKENLGGTCLNVGCIPTKAFVKSSEVFHNALNSEEFGFTADNLQVDMK
EEHHCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEECCCCEEHHH
KVVKRKDDVKGRLVNGIEYLLEANGVRLIKGEASFLDNKNIIVKRGKDEYTIESKDIIIA
HHHCCCCCCCCHHHHHHHHHHHCCCEEEEECCHHCCCCCCEEEEECCCCEEEECCCEEEE
TGSKISKIDIKGIDMPFVLNSTTALNNEKLPESITIIGGGVIGMEFAFIYSNFGVKVNVV
CCCCEEEEEEECCCCCEEECCCCCCCCCCCCCEEEEEECCHHHEEEEEEEECCCEEEEEH
EYADRLLLMMDKDISEEIKDIATDKGINVYTSSKVTEIKKSEDGDAIVSFEKDGEEKLLV
HHHCCEEEEECCCHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCEEEEECCCCCCEEEE
SENVLVAIGREPNIDGLNIENINLELNDNRRGIKVNSKLETNVEGIYAIGDVNNIMQLAH
ECCEEEEECCCCCCCCEEEEEEEEEECCCCCCEEECCEEECCCCEEEEECCHHHHHHHHH
VASHQGMIAVDNILGQNKEMSYDHVPNVIFTVPEIASVGMNEDKCLKEELNIKISKFPYS
HHHCCCEEEEHHHHCCCCCCCCCCCCCEEEECCHHHHCCCCHHHHHHHHCCEEEEECCCC
ANGKALTMGEEKGFIKIIKDINNNKIVGASIIGADASSLISTLTLIIKNNISEEKICETI
CCCCEEEECCCCCEEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHH
FAHPTTGEVIHESFLGLSIGAIHNHE
HCCCCCHHHHHHHHHCEEEEEEECCC
>Mature Secondary Structure
MDIKLEKLSGHNKSAKVGKISVIEGEQVKKGHVLMQLETSKGNTPFKATFDFKIDKIKVS
CCEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCEEEEEEEEEEEEEEC
EGDEVEIGQILFLATGENINVNSNTPKVDYFGSMLKGKKENVTADLTIIGGGPGGYVAAI
CCCCEEECEEEEEEECCEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEE
YAAKKGLNTVIIEKENLGGTCLNVGCIPTKAFVKSSEVFHNALNSEEFGFTADNLQVDMK
EEHHCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEECCCCEEHHH
KVVKRKDDVKGRLVNGIEYLLEANGVRLIKGEASFLDNKNIIVKRGKDEYTIESKDIIIA
HHHCCCCCCCCHHHHHHHHHHHCCCEEEEECCHHCCCCCCEEEEECCCCEEEECCCEEEE
TGSKISKIDIKGIDMPFVLNSTTALNNEKLPESITIIGGGVIGMEFAFIYSNFGVKVNVV
CCCCEEEEEEECCCCCEEECCCCCCCCCCCCCEEEEEECCHHHEEEEEEEECCCEEEEEH
EYADRLLLMMDKDISEEIKDIATDKGINVYTSSKVTEIKKSEDGDAIVSFEKDGEEKLLV
HHHCCEEEEECCCHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCEEEEECCCCCCEEEE
SENVLVAIGREPNIDGLNIENINLELNDNRRGIKVNSKLETNVEGIYAIGDVNNIMQLAH
ECCEEEEECCCCCCCCEEEEEEEEEECCCCCCEEECCEEECCCCEEEEECCHHHHHHHHH
VASHQGMIAVDNILGQNKEMSYDHVPNVIFTVPEIASVGMNEDKCLKEELNIKISKFPYS
HHHCCCEEEEHHHHCCCCCCCCCCCCCEEEECCHHHHCCCCHHHHHHHHCCEEEEECCCC
ANGKALTMGEEKGFIKIIKDINNNKIVGASIIGADASSLISTLTLIIKNNISEEKICETI
CCCCEEEECCCCCEEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHH
FAHPTTGEVIHESFLGLSIGAIHNHE
HCCCCCHHHHHHHHHCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377; 1936936 [H]