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The map label for this gene is pfkA [H]

Identifier: 187918582

GI number: 187918582

Start: 772661

End: 774004

Strand: Direct

Name: pfkA [H]

Synonym: BH0727

Alternate gene names: 187918582

Gene position: 772661-774004 (Clockwise)

Preceding gene: 187918581

Following gene: 187918588

Centisome position: 83.77

GC content: 33.78

Gene sequence:

>1344_bases
ATGCATAGAATTAGGAATGAGAATTTAGATTTTAAAATAGAAAATTTAGGAGAATGTAAACAAGATAATCCTTTGATTAG
TTTTTATGCTAAGGAGAGTCATGTTCACTTTACTGATGAGACTAGTAAGATTAGATTGAATGTTTATAAAAATGAAGAGG
GTTGGGAGCAATATGAGAATGTTTTTTTAGAGAAAGCTGGTCCTAGATATAAGATTTATTTTGATCCAAGTCATGTTAAG
GCGGCAATTACTACTTGTGGAGGTCTTTGTCCAGGTTTTAATGATGTTATTCGTTCAATTGTTAGAACTTTATGGAAAGT
ATATGGAGTTTATAATATTTATGGAGTTAAATTCGGATATCAGGGACTTTTGCCAGAGTCTAATTTGAGTTTTATGAAGC
TAAATCCTGATGTAGTAGATGATATTAATCAGCTTGGTGGGACAATACTTGGTTCATCAAGAGGGGGGATTAAACCTGTT
GAAATAGTTGATACTTTGGAGAGGATGGAAATTAATATGCTCTTTAATATCGGTGGAGATGGAACACAAAAGGGTTCTGT
TTTAATTGCTGAGGAAATAGAGAGAAGAAATTTGAAAATAGCTGTTGTAGGTATTCCAAAGACAATAGATAATGATTTGA
TGTTTGTTCAAAAATCTTTTGGATTTGAAACGGCTGTGGAGCAAGCAGTTGCTGCGGTTGCTGGTGCTCACTTTGAGGCA
AATAGTGCTTATAATGGAATTGGTCTTGTTAAGGTTATGGGTAGAGATTCTGGGTTTATTGCTGCTTATACTGCTTTATC
ATCTAATGATGTTAATTTTTGTTTAATCCCAGAACTAGATTTTGATATTGAGGGGCCTAATGGGCTTCTTGCACATCTTG
AAAGACGCCTTTTGGCAAAAGAGAATTTGGATGAGATGCCCCATGCAGTAATATTAATAGCAGAGGGAGCTGGACAAAAA
TATTTTGATCCTGGTAGTCGCAAAAGAGATGATTCTGGTAATTTGCTTTATGAAGATATTGGACTTTATATTAAAGACAA
GGTTAATACGTATTTTAAATCAAAAAATATTCCAGTTAATCTTAAATATATTGATCCTAGTTATATTATTAGAAGCTCAC
CAGCTAATGCTAGTGATTCCCTTTATTGTGCTCGTCTTGGCTCAAATGCTGTTCATGCTGCTATGGCAGGTAAGACAAAA
TTATTAATTAGCTTATGGAGTACAAAATTTGTACATATTCCAATAAAAATGGCAGTAATTGCTAGAAATAAAGTCAATAT
AAATGGTTCTTTCTGGCGGGATGTTCTTTCAAGTACTGGTCAGCCATTTAGCATGAAGAACTAA

Upstream 100 bases:

>100_bases
CTTATATTTATGATGATATATACGATATGATTAAAGAACAAAGTCAATTTATTAGGAAATAATTGAATTATAATAATACT
TATTATGGAGGAGGAACTTA

Downstream 100 bases:

>100_bases
AATAAAGGTAATTTAAGTTTTCTTTTATTTTTCTTTTAGTTATTTTGCTGCATTGCCCGCAATATTTAATGCATCCCAAG
TTTTTGAGAATGGTGGAGAA

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MHRIRNENLDFKIENLGECKQDNPLISFYAKESHVHFTDETSKIRLNVYKNEEGWEQYENVFLEKAGPRYKIYFDPSHVK
AAITTCGGLCPGFNDVIRSIVRTLWKVYGVYNIYGVKFGYQGLLPESNLSFMKLNPDVVDDINQLGGTILGSSRGGIKPV
EIVDTLERMEINMLFNIGGDGTQKGSVLIAEEIERRNLKIAVVGIPKTIDNDLMFVQKSFGFETAVEQAVAAVAGAHFEA
NSAYNGIGLVKVMGRDSGFIAAYTALSSNDVNFCLIPELDFDIEGPNGLLAHLERRLLAKENLDEMPHAVILIAEGAGQK
YFDPGSRKRDDSGNLLYEDIGLYIKDKVNTYFKSKNIPVNLKYIDPSYIIRSSPANASDSLYCARLGSNAVHAAMAGKTK
LLISLWSTKFVHIPIKMAVIARNKVNINGSFWRDVLSSTGQPFSMKN

Sequences:

>Translated_447_residues
MHRIRNENLDFKIENLGECKQDNPLISFYAKESHVHFTDETSKIRLNVYKNEEGWEQYENVFLEKAGPRYKIYFDPSHVK
AAITTCGGLCPGFNDVIRSIVRTLWKVYGVYNIYGVKFGYQGLLPESNLSFMKLNPDVVDDINQLGGTILGSSRGGIKPV
EIVDTLERMEINMLFNIGGDGTQKGSVLIAEEIERRNLKIAVVGIPKTIDNDLMFVQKSFGFETAVEQAVAAVAGAHFEA
NSAYNGIGLVKVMGRDSGFIAAYTALSSNDVNFCLIPELDFDIEGPNGLLAHLERRLLAKENLDEMPHAVILIAEGAGQK
YFDPGSRKRDDSGNLLYEDIGLYIKDKVNTYFKSKNIPVNLKYIDPSYIIRSSPANASDSLYCARLGSNAVHAAMAGKTK
LLISLWSTKFVHIPIKMAVIARNKVNINGSFWRDVLSSTGQPFSMKN
>Mature_447_residues
MHRIRNENLDFKIENLGECKQDNPLISFYAKESHVHFTDETSKIRLNVYKNEEGWEQYENVFLEKAGPRYKIYFDPSHVK
AAITTCGGLCPGFNDVIRSIVRTLWKVYGVYNIYGVKFGYQGLLPESNLSFMKLNPDVVDDINQLGGTILGSSRGGIKPV
EIVDTLERMEINMLFNIGGDGTQKGSVLIAEEIERRNLKIAVVGIPKTIDNDLMFVQKSFGFETAVEQAVAAVAGAHFEA
NSAYNGIGLVKVMGRDSGFIAAYTALSSNDVNFCLIPELDFDIEGPNGLLAHLERRLLAKENLDEMPHAVILIAEGAGQK
YFDPGSRKRDDSGNLLYEDIGLYIKDKVNTYFKSKNIPVNLKYIDPSYIIRSSPANASDSLYCARLGSNAVHAAMAGKTK
LLISLWSTKFVHIPIKMAVIARNKVNINGSFWRDVLSSTGQPFSMKN

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453768, Length=368, Percent_Identity=25.2717391304348, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI266453748, Length=368, Percent_Identity=25.2717391304348, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI4505749, Length=368, Percent_Identity=25.2717391304348, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI266453619, Length=366, Percent_Identity=24.8633879781421, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI11321601, Length=393, Percent_Identity=23.4096692111959, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI48762920, Length=372, Percent_Identity=23.9247311827957, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1790350, Length=215, Percent_Identity=31.6279069767442, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25147584, Length=366, Percent_Identity=25.4098360655738, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17558788, Length=343, Percent_Identity=25.0728862973761, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321679, Length=269, Percent_Identity=25.6505576208178, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17647809, Length=394, Percent_Identity=23.8578680203046, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652337, Length=394, Percent_Identity=23.8578680203046, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28573326, Length=394, Percent_Identity=23.3502538071066, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 49729; Mature: 49729

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRIRNENLDFKIENLGECKQDNPLISFYAKESHVHFTDETSKIRLNVYKNEEGWEQYEN
CCCCCCCCCCEEECCCCCCCCCCCEEEEEEECCEEEEECCCCEEEEEEEECCCHHHHHHH
VFLEKAGPRYKIYFDPSHVKAAITTCGGLCPGFNDVIRSIVRTLWKVYGVYNIYGVKFGY
HHHCCCCCEEEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECC
QGLLPESNLSFMKLNPDVVDDINQLGGTILGSSRGGIKPVEIVDTLERMEINMLFNIGGD
CCCCCCCCCEEEEECCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHEEEEEEEEECCC
GTQKGSVLIAEEIERRNLKIAVVGIPKTIDNDLMFVQKSFGFETAVEQAVAAVAGAHFEA
CCCCCCEEEEEHHHHCCEEEEEEECCCCCCCCEEEEEHHCCHHHHHHHHHHHHHCCCEEC
NSAYNGIGLVKVMGRDSGFIAAYTALSSNDVNFCLIPELDFDIEGPNGLLAHLERRLLAK
CCCCCCEEEEEEECCCCCEEEEEEEECCCCCCEEEECCCCCEECCCCHHHHHHHHHHHHH
ENLDEMPHAVILIAEGAGQKYFDPGSRKRDDSGNLLYEDIGLYIKDKVNTYFKSKNIPVN
CCHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCEEEEECCHHHEECCCCEEE
LKYIDPSYIIRSSPANASDSLYCARLGSNAVHAAMAGKTKLLISLWSTKFVHIPIKMAVI
EEEECCCEEEECCCCCCCCCEEEEECCCCCEEHHHCCCCEEEEEEECCEEEEEEEEEEEE
ARNKVNINGSFWRDVLSSTGQPFSMKN
ECCEEECCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MHRIRNENLDFKIENLGECKQDNPLISFYAKESHVHFTDETSKIRLNVYKNEEGWEQYEN
CCCCCCCCCCEEECCCCCCCCCCCEEEEEEECCEEEEECCCCEEEEEEEECCCHHHHHHH
VFLEKAGPRYKIYFDPSHVKAAITTCGGLCPGFNDVIRSIVRTLWKVYGVYNIYGVKFGY
HHHCCCCCEEEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECC
QGLLPESNLSFMKLNPDVVDDINQLGGTILGSSRGGIKPVEIVDTLERMEINMLFNIGGD
CCCCCCCCCEEEEECCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHEEEEEEEEECCC
GTQKGSVLIAEEIERRNLKIAVVGIPKTIDNDLMFVQKSFGFETAVEQAVAAVAGAHFEA
CCCCCCEEEEEHHHHCCEEEEEEECCCCCCCCEEEEEHHCCHHHHHHHHHHHHHCCCEEC
NSAYNGIGLVKVMGRDSGFIAAYTALSSNDVNFCLIPELDFDIEGPNGLLAHLERRLLAK
CCCCCCEEEEEEECCCCCEEEEEEEECCCCCCEEEECCCCCEECCCCHHHHHHHHHHHHH
ENLDEMPHAVILIAEGAGQKYFDPGSRKRDDSGNLLYEDIGLYIKDKVNTYFKSKNIPVN
CCHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCEEEEECCHHHEECCCCEEE
LKYIDPSYIIRSSPANASDSLYCARLGSNAVHAAMAGKTKLLISLWSTKFVHIPIKMAVI
EEEECCCEEEECCCCCCCCCEEEEECCCCCEEHHHCCCCEEEEEEECCEEEEEEEEEEEE
ARNKVNINGSFWRDVLSSTGQPFSMKN
ECCEEECCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]