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The map label for this gene is mrdA [H]

Identifier: 187918573

GI number: 187918573

Start: 761844

End: 763646

Strand: Direct

Name: mrdA [H]

Synonym: BH0718

Alternate gene names: 187918573

Gene position: 761844-763646 (Clockwise)

Preceding gene: 187918572

Following gene: 187918574

Centisome position: 82.6

GC content: 32.28

Gene sequence:

>1803_bases
ATGAAAGTTATTTTGAGCGGCAGGTATAAGTTTGGGCTGTTACTTTTATCTTTCGTTTTTTTTTCTTATCTTTTTACTTT
ATTTAAAATGCAGATTGGGAAACACTTGTTGTATGATAGAGAAGCAACGGTTCTTTTATCTAGGGTTGAGAAAATCAAGG
CTTCAAGGGGTGAAATTCTAGATTCAAATTTTAATGTTCTTGCGAATAATCTTACGGCATTTGTTTTAAAAATTAGTTTG
GAACAATATTACGGCATGTCTCTTGAGGATAGGGACGGAATGTTAAATTTTTTGTCAAGTATTCTAGGTATTGAAAGAGA
ACTTATTCTCTCTAAAATTGAGGCTACTAGAGGGTATTTGAAAGATGTAGAAATAGTTGAACTTAGTCCAGAAATGTTGT
TTAGAGTTGCTGAGAAGAGAAGTTATTATCCTGCACTTTTATGGACATATTCTTTTAAAAGAAATTATTTGGTGGATGAT
TCTCATTCCCATCCTATTGGGTATGTTGGTAGGATTAATCAAAGGGAACTTCGTTCTTTTTATAATGTTCAAGGGTATGA
TAATAATTCTACAATAGGAAAATTAGGCATTGAACAAGTTTATGATAGTTATATTAGAGGGAAGGAAGGTTTAATTAGGT
ATAAGGTAGATTCTAGAGAGAGAAAAATGGATAGTGGTTCTATTATAGAGCGTATGATTCCTGGTAATAATATTGTTTTA
AATATTAATAAGGATATTCAACTGCTTGCTAAGAATACTTTAGGTAAGAGATACGGAACTATAGTAGTATTAAAACCCGC
AACAGGAAGTGTGTTAGCACTTCATAGTTATCCTTACTATTCAATGAATGATGTTTATAATAAGCATTCTAAGGAAGATT
ATTCTTTTCTCAATAGGGCAATACAATCAGTTTATCCTCCCGCATCTATTTTTAAATTGGTTATGACTACGGCGTTATTA
GAAGAAAAGGTCCTTGATAAGGGTAAAAAAATTCATTGCCCGGGCTATTTTAGAGTGGGGAATAGAGTTTTTCATTGTTG
GCAACGTGGTGGTCATGGTTATGTCGATTTAGAACATGCTGTTGCTCATTCATGTAATGTTTATTTTTATATATTGGGAC
TTAAGTACCTTGGTATTGAGAAGATTTTTAAGTATGCAAAAGAGTATGGATTTGGAGAGAAAACTGGGATTGATTTGCCA
AATGAGGTTTCTGGCATTCTTCCAAATCCAGAATGGAAGAAAAAAACTTTTAATCAACCTTGGGTAGGTGGTGATACTGT
AAATTGTTCAATAGGCCAAGGTTTTTTAAGTGCTACTTCTATTCAGATTGCTAATATGATAGCTATGATTGCAAATGAAG
GTGTCATTTATAAACCAAGAATTGTTAGTAAGATTTTAAATGGAACTACTAATGAAGTTATTCTTGAAAATGTTCCTGAA
ATTCTTAAGAAAACAGATATTATTAGTAGAAATACGTTTAAGGTTTTAAAAAAATATATGAGAAATGTCATAACTTATGG
TACTGCTAGAAATTCAGTTCTCACAAAAGCTGTTGCTGTTGTAGGAAAGACAGGTACAGGTCAAACTGGTGTTACTGGAC
TTGAGAATAGTTCTTTTGTTAGTCTTGCTCCTTATAATGCCCCACCTAGTGAACAAATTATTATTTTTAGTTTGGTTGAA
GGGAGAAGTAACACAGATAATATGTGGTCTGCTAAATCCGTAGATTTAATGATGCAAGGGATTTTTACTAATCAGAGTTA
TGAGGATATTCTTAGAGAATATAGACCATGGTATATTAGGTAA

Upstream 100 bases:

>100_bases
TGATCTTAATCTTGTCATAAATATAGTATTTTTAAATTTTTTATATCCGATTTTAAATTATTGCACGAAAAGTCTTTATA
CTATTAAAGAGGAATATTAA

Downstream 100 bases:

>100_bases
GGTAAATGGCTGTTTTTAGAAAAAGTTATGATAGTTTGGCATTATTTAGTTTGGTTATGATATCTTTTATTGGAGTTTTG
CTTATATATTCTAGTGATTA

Product: cell elongation specific D,D-transpeptidase

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MKVILSGRYKFGLLLLSFVFFSYLFTLFKMQIGKHLLYDREATVLLSRVEKIKASRGEILDSNFNVLANNLTAFVLKISL
EQYYGMSLEDRDGMLNFLSSILGIERELILSKIEATRGYLKDVEIVELSPEMLFRVAEKRSYYPALLWTYSFKRNYLVDD
SHSHPIGYVGRINQRELRSFYNVQGYDNNSTIGKLGIEQVYDSYIRGKEGLIRYKVDSRERKMDSGSIIERMIPGNNIVL
NINKDIQLLAKNTLGKRYGTIVVLKPATGSVLALHSYPYYSMNDVYNKHSKEDYSFLNRAIQSVYPPASIFKLVMTTALL
EEKVLDKGKKIHCPGYFRVGNRVFHCWQRGGHGYVDLEHAVAHSCNVYFYILGLKYLGIEKIFKYAKEYGFGEKTGIDLP
NEVSGILPNPEWKKKTFNQPWVGGDTVNCSIGQGFLSATSIQIANMIAMIANEGVIYKPRIVSKILNGTTNEVILENVPE
ILKKTDIISRNTFKVLKKYMRNVITYGTARNSVLTKAVAVVGKTGTGQTGVTGLENSSFVSLAPYNAPPSEQIIIFSLVE
GRSNTDNMWSAKSVDLMMQGIFTNQSYEDILREYRPWYIR

Sequences:

>Translated_600_residues
MKVILSGRYKFGLLLLSFVFFSYLFTLFKMQIGKHLLYDREATVLLSRVEKIKASRGEILDSNFNVLANNLTAFVLKISL
EQYYGMSLEDRDGMLNFLSSILGIERELILSKIEATRGYLKDVEIVELSPEMLFRVAEKRSYYPALLWTYSFKRNYLVDD
SHSHPIGYVGRINQRELRSFYNVQGYDNNSTIGKLGIEQVYDSYIRGKEGLIRYKVDSRERKMDSGSIIERMIPGNNIVL
NINKDIQLLAKNTLGKRYGTIVVLKPATGSVLALHSYPYYSMNDVYNKHSKEDYSFLNRAIQSVYPPASIFKLVMTTALL
EEKVLDKGKKIHCPGYFRVGNRVFHCWQRGGHGYVDLEHAVAHSCNVYFYILGLKYLGIEKIFKYAKEYGFGEKTGIDLP
NEVSGILPNPEWKKKTFNQPWVGGDTVNCSIGQGFLSATSIQIANMIAMIANEGVIYKPRIVSKILNGTTNEVILENVPE
ILKKTDIISRNTFKVLKKYMRNVITYGTARNSVLTKAVAVVGKTGTGQTGVTGLENSSFVSLAPYNAPPSEQIIIFSLVE
GRSNTDNMWSAKSVDLMMQGIFTNQSYEDILREYRPWYIR
>Mature_600_residues
MKVILSGRYKFGLLLLSFVFFSYLFTLFKMQIGKHLLYDREATVLLSRVEKIKASRGEILDSNFNVLANNLTAFVLKISL
EQYYGMSLEDRDGMLNFLSSILGIERELILSKIEATRGYLKDVEIVELSPEMLFRVAEKRSYYPALLWTYSFKRNYLVDD
SHSHPIGYVGRINQRELRSFYNVQGYDNNSTIGKLGIEQVYDSYIRGKEGLIRYKVDSRERKMDSGSIIERMIPGNNIVL
NINKDIQLLAKNTLGKRYGTIVVLKPATGSVLALHSYPYYSMNDVYNKHSKEDYSFLNRAIQSVYPPASIFKLVMTTALL
EEKVLDKGKKIHCPGYFRVGNRVFHCWQRGGHGYVDLEHAVAHSCNVYFYILGLKYLGIEKIFKYAKEYGFGEKTGIDLP
NEVSGILPNPEWKKKTFNQPWVGGDTVNCSIGQGFLSATSIQIANMIAMIANEGVIYKPRIVSKILNGTTNEVILENVPE
ILKKTDIISRNTFKVLKKYMRNVITYGTARNSVLTKAVAVVGKTGTGQTGVTGLENSSFVSLAPYNAPPSEQIIIFSLVE
GRSNTDNMWSAKSVDLMMQGIFTNQSYEDILREYRPWYIR

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=591, Percent_Identity=27.4111675126904, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1786272, Length=518, Percent_Identity=22.007722007722, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 68245; Mature: 68245

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVILSGRYKFGLLLLSFVFFSYLFTLFKMQIGKHLLYDREATVLLSRVEKIKASRGEIL
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEE
DSNFNVLANNLTAFVLKISLEQYYGMSLEDRDGMLNFLSSILGIERELILSKIEATRGYL
CCCCHHHHHHHEEEHHEEEHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KDVEIVELSPEMLFRVAEKRSYYPALLWTYSFKRNYLVDDSHSHPIGYVGRINQRELRSF
CCCEEEECCHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCCCCCCCEECCCCHHHHHHH
YNVQGYDNNSTIGKLGIEQVYDSYIRGKEGLIRYKVDSRERKMDSGSIIERMIPGNNIVL
HCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECHHHHCCCCCHHHHHHCCCCCEEE
NINKDIQLLAKNTLGKRYGTIVVLKPATGSVLALHSYPYYSMNDVYNKHSKEDYSFLNRA
EECCCHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHCCCHHHHHHHHH
IQSVYPPASIFKLVMTTALLEEKVLDKGKKIHCPGYFRVGNRVFHCWQRGGHGYVDLEHA
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCCCCEEEHHHH
VAHSCNVYFYILGLKYLGIEKIFKYAKEYGFGEKTGIDLPNEVSGILPNPEWKKKTFNQP
HHHCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCHHHHCCCCC
WVGGDTVNCSIGQGFLSATSIQIANMIAMIANEGVIYKPRIVSKILNGTTNEVILENVPE
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHCCCCCHHHHHHHHH
ILKKTDIISRNTFKVLKKYMRNVITYGTARNSVLTKAVAVVGKTGTGQTGVTGLENSSFV
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
SLAPYNAPPSEQIIIFSLVEGRSNTDNMWSAKSVDLMMQGIFTNQSYEDILREYRPWYIR
EEECCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVILSGRYKFGLLLLSFVFFSYLFTLFKMQIGKHLLYDREATVLLSRVEKIKASRGEIL
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEE
DSNFNVLANNLTAFVLKISLEQYYGMSLEDRDGMLNFLSSILGIERELILSKIEATRGYL
CCCCHHHHHHHEEEHHEEEHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KDVEIVELSPEMLFRVAEKRSYYPALLWTYSFKRNYLVDDSHSHPIGYVGRINQRELRSF
CCCEEEECCHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCCCCCCCEECCCCHHHHHHH
YNVQGYDNNSTIGKLGIEQVYDSYIRGKEGLIRYKVDSRERKMDSGSIIERMIPGNNIVL
HCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECHHHHCCCCCHHHHHHCCCCCEEE
NINKDIQLLAKNTLGKRYGTIVVLKPATGSVLALHSYPYYSMNDVYNKHSKEDYSFLNRA
EECCCHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHCCCHHHHHHHHH
IQSVYPPASIFKLVMTTALLEEKVLDKGKKIHCPGYFRVGNRVFHCWQRGGHGYVDLEHA
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCCCCEEEHHHH
VAHSCNVYFYILGLKYLGIEKIFKYAKEYGFGEKTGIDLPNEVSGILPNPEWKKKTFNQP
HHHCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCHHHHCCCCC
WVGGDTVNCSIGQGFLSATSIQIANMIAMIANEGVIYKPRIVSKILNGTTNEVILENVPE
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHCCCCCHHHHHHHHH
ILKKTDIISRNTFKVLKKYMRNVITYGTARNSVLTKAVAVVGKTGTGQTGVTGLENSSFV
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
SLAPYNAPPSEQIIIFSLVEGRSNTDNMWSAKSVDLMMQGIFTNQSYEDILREYRPWYIR
EEECCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]