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The map label for this gene is trmD [H]

Identifier: 187918553

GI number: 187918553

Start: 744006

End: 744725

Strand: Direct

Name: trmD [H]

Synonym: BH0698

Alternate gene names: 187918553

Gene position: 744006-744725 (Clockwise)

Preceding gene: 187918552

Following gene: 187918554

Centisome position: 80.67

GC content: 33.75

Gene sequence:

>720_bases
ATGAAAATTACGATTCTCTCTTTATTTCCTTCAATCATTACCCCATTTTTTGAAAATTCAATAATGAAAAAAGTTGTAGA
TAGAGGTATCATAAGTTATGAGGTCATATCTATTCGTGACTTTTCTGATGATAAACATAAAAGATGTGATGATATTCCTT
ATGGCGGAGGTGCAGGTATGGTTTTGAAAGCCCCGCCTATCTCTGCTGCTCTTGAGTATGTAAATGCAAAATCAAAAACC
ACAATCTTTGTAAGTCCATCTGGATTGAAATATACTCAAAAATTGGCTTATGACTTGTCAAAGAAGAGTGAACTTGTTAT
AATTTGTGGAAGATATGAAGGGCTTGATCAACGCGTGATTGATTTATATGTTGATCTTGAAGTTTCAGTTGGAGATTATG
TATTGTCTTCAGGTGAAGTGGCTGCTCTTGTTATAATAGATAGTGTATATAGATTGTTAGATGGGGCAATAAATCCAAGT
TCCTTGCTTGAGGAATCTTTCAGTTTCGATTGTGGCCTACTTGAGTACCCTCATTATACTAGGCCTTATGAATTTAAGGG
GTTGGAAGTTCCTGATGTGCTTCTTTCAGGTCATCACGAGGAAATAAAAAAATGGCGGTTTATTAAGTCTGTTGAAAAAA
CAAAGAAAAATAGATATGATTTGTACCTTAAATATTTGGAAATGATAGGAGAAAATGATGGATTTAGTAAGAAAAATTGA

Upstream 100 bases:

>100_bases
TTGGAAGTAAATTGTTTTTTGTTCCCTTTTTAAATGTTTATCTTGGAGATATTAATAGGGAATTGAAAACTATTGAGCTT
AAGGTATTAGATCTCTTAAA

Downstream 100 bases:

>100_bases
GGCTAAAGGAAAGAGAACAGAAAGTTTTGATTTTAGGGCAGGAGATACTATACGTGTTAGTTATAGGATAATTGAAGGCA
CTAATGAGAGAGTTCAAAAT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKITILSLFPSIITPFFENSIMKKVVDRGIISYEVISIRDFSDDKHKRCDDIPYGGGAGMVLKAPPISAALEYVNAKSKT
TIFVSPSGLKYTQKLAYDLSKKSELVIICGRYEGLDQRVIDLYVDLEVSVGDYVLSSGEVAALVIIDSVYRLLDGAINPS
SLLEESFSFDCGLLEYPHYTRPYEFKGLEVPDVLLSGHHEEIKKWRFIKSVEKTKKNRYDLYLKYLEMIGENDGFSKKN

Sequences:

>Translated_239_residues
MKITILSLFPSIITPFFENSIMKKVVDRGIISYEVISIRDFSDDKHKRCDDIPYGGGAGMVLKAPPISAALEYVNAKSKT
TIFVSPSGLKYTQKLAYDLSKKSELVIICGRYEGLDQRVIDLYVDLEVSVGDYVLSSGEVAALVIIDSVYRLLDGAINPS
SLLEESFSFDCGLLEYPHYTRPYEFKGLEVPDVLLSGHHEEIKKWRFIKSVEKTKKNRYDLYLKYLEMIGENDGFSKKN
>Mature_239_residues
MKITILSLFPSIITPFFENSIMKKVVDRGIISYEVISIRDFSDDKHKRCDDIPYGGGAGMVLKAPPISAALEYVNAKSKT
TIFVSPSGLKYTQKLAYDLSKKSELVIICGRYEGLDQRVIDLYVDLEVSVGDYVLSSGEVAALVIIDSVYRLLDGAINPS
SLLEESFSFDCGLLEYPHYTRPYEFKGLEVPDVLLSGHHEEIKKWRFIKSVEKTKKNRYDLYLKYLEMIGENDGFSKKN

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=225, Percent_Identity=41.7777777777778, Blast_Score=184, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27048; Mature: 27048

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITILSLFPSIITPFFENSIMKKVVDRGIISYEVISIRDFSDDKHKRCDDIPYGGGAGM
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHCCCCCCCCCCCE
VLKAPPISAALEYVNAKSKTTIFVSPSGLKYTQKLAYDLSKKSELVIICGRYEGLDQRVI
EEECCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCEEE
DLYVDLEVSVGDYVLSSGEVAALVIIDSVYRLLDGAINPSSLLEESFSFDCGLLEYPHYT
EEEEEEEEECCCEEECCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEECCCCCC
RPYEFKGLEVPDVLLSGHHEEIKKWRFIKSVEKTKKNRYDLYLKYLEMIGENDGFSKKN
CCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKITILSLFPSIITPFFENSIMKKVVDRGIISYEVISIRDFSDDKHKRCDDIPYGGGAGM
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHCCCCCCCCCCCE
VLKAPPISAALEYVNAKSKTTIFVSPSGLKYTQKLAYDLSKKSELVIICGRYEGLDQRVI
EEECCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCEEE
DLYVDLEVSVGDYVLSSGEVAALVIIDSVYRLLDGAINPSSLLEESFSFDCGLLEYPHYT
EEEEEEEEECCCEEECCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEECCCCCC
RPYEFKGLEVPDVLLSGHHEEIKKWRFIKSVEKTKKNRYDLYLKYLEMIGENDGFSKKN
CCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA