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The map label for this gene is tig

Identifier: 187918470

GI number: 187918470

Start: 643425

End: 644771

Strand: Direct

Name: tig

Synonym: BH0610

Alternate gene names: 187918470

Gene position: 643425-644771 (Clockwise)

Preceding gene: 187918469

Following gene: 187918471

Centisome position: 69.76

GC content: 28.73

Gene sequence:

>1347_bases
GTGATATTAAATGATGATGTGAAATTGATTCCTGGTTCTAAGGTTGAAGCTGTTATTAAAATTTCAAAAGAATTTGTTAA
AGGTAAATACAACGAGATTTTACAAGATTATTCTTCTCGTCTTAAGATTAAAGGTTTTAGGATAGGAAAAGTTCCTTTTA
GTATTATTGAAGGCAAATATTCTGATAATATAAGAGCTCTTACTATAGAAAAACTTATTCATAAGTCTTTAGAAGAATTT
TTTAAAAGTGCAACTTATAAACCATTAGGTTATGCTGTTCCTAAAATATTAGATGAAAAATTAGAAATAGATTTCAATAA
AGATTTTGAGTTTACTGTTGTTTATGAGGCTTATCCTGAATTTGAGATACCTGATATCTCTAATGTTGAGGTAGAAATTC
CAGAAGTTACCGTTTCTGATTCTGATGTAGAGGACGAGCTTAAATTATTACAACTTGAAAATGCAATGGTTGTTGATGAT
AGTGGTGATGTTAAGGTAGGCAGTATTGTTAGAGTAGATTTTGTGGAACTTGATGATTCTTTAAGTGAGATCTTAACAAC
TAAGAGGCAAGATTTTGTTTTTACTGTTGGTGAGTCTAATAATTATTATGGATTTGATAATGACATCATTGGAATGAAGA
AGGATGAAGAGAAAATAATAGAGAAAAATTATAGTGCTGATTATAAATTTAGTGAGCTTGCTAATTCTTTTAAGAGATTA
AAGATTATTATTAAAGATATAAAGAAGCGTGATATTCCTGAACTTGATGATGATTTTGCAAAGGATATTAAAGATAGTTT
TAATACATTAGAAGAGCTTAAGGCCCACATAAGAGAGAATATGTTAAGGCTTGTTAAGGAAAAGAGAGAATCTCTTAAGC
TTTCAAAATTGTTGTCCGATGTTTCAGAGAAGCTAAACATAGAGATTCCCTCTGCTATGTTTGAGGCTGAACTTAAAAAT
GTTGTAAACGAATTTTCAACTCAAAATAAGATTGATCTTAAGAAGTTAAATGATTCTTCTATGGGGTTGGAAGGTGTTAG
TGATGTTTTTAAGGAGAATGTACTTAAGAAGTTAAAGTCTAAGTTAGTTTTCCAAAAGATAGTAGATAATGATTTAACAG
AAATTACAGATGCTGATTTAGAAGATGAACTTGTTAAACAGGCTGAGGATACAAAGATGAGACTTTCGGAGATTAAGAAA
TTTTATCAAGAGAAAAATTTGCTTGGAATTTTAAAAGATGAAATTAAGAGGCAAAAGGTTAAAGATAAAATTTTGAAAAA
TGTGAAGGAAATTAAACTTAAAGAAATTGCCTTTAGAGATTTTATTAATTATAAAACAGGTGAATAA

Upstream 100 bases:

>100_bases
GAGTGTCACCTTGCCAAGGTGGAAGTCGCGGGTTCAAATCCCGTCTTTCGCTTAAATTTAGTAATAATTAACATTAGTGC
ATTTTAATTAGAGGAATAAT

Downstream 100 bases:

>100_bases
TGATAAGAGAATATATGCATAATTTAGTGCCTACTGTTGTGGAGTATACTGGAAATTATGAGCGCGTATTTGATATATAT
TCAAGATTGTTAAGGGATAG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MILNDDVKLIPGSKVEAVIKISKEFVKGKYNEILQDYSSRLKIKGFRIGKVPFSIIEGKYSDNIRALTIEKLIHKSLEEF
FKSATYKPLGYAVPKILDEKLEIDFNKDFEFTVVYEAYPEFEIPDISNVEVEIPEVTVSDSDVEDELKLLQLENAMVVDD
SGDVKVGSIVRVDFVELDDSLSEILTTKRQDFVFTVGESNNYYGFDNDIIGMKKDEEKIIEKNYSADYKFSELANSFKRL
KIIIKDIKKRDIPELDDDFAKDIKDSFNTLEELKAHIRENMLRLVKEKRESLKLSKLLSDVSEKLNIEIPSAMFEAELKN
VVNEFSTQNKIDLKKLNDSSMGLEGVSDVFKENVLKKLKSKLVFQKIVDNDLTEITDADLEDELVKQAEDTKMRLSEIKK
FYQEKNLLGILKDEIKRQKVKDKILKNVKEIKLKEIAFRDFINYKTGE

Sequences:

>Translated_448_residues
MILNDDVKLIPGSKVEAVIKISKEFVKGKYNEILQDYSSRLKIKGFRIGKVPFSIIEGKYSDNIRALTIEKLIHKSLEEF
FKSATYKPLGYAVPKILDEKLEIDFNKDFEFTVVYEAYPEFEIPDISNVEVEIPEVTVSDSDVEDELKLLQLENAMVVDD
SGDVKVGSIVRVDFVELDDSLSEILTTKRQDFVFTVGESNNYYGFDNDIIGMKKDEEKIIEKNYSADYKFSELANSFKRL
KIIIKDIKKRDIPELDDDFAKDIKDSFNTLEELKAHIRENMLRLVKEKRESLKLSKLLSDVSEKLNIEIPSAMFEAELKN
VVNEFSTQNKIDLKKLNDSSMGLEGVSDVFKENVLKKLKSKLVFQKIVDNDLTEITDADLEDELVKQAEDTKMRLSEIKK
FYQEKNLLGILKDEIKRQKVKDKILKNVKEIKLKEIAFRDFINYKTGE
>Mature_448_residues
MILNDDVKLIPGSKVEAVIKISKEFVKGKYNEILQDYSSRLKIKGFRIGKVPFSIIEGKYSDNIRALTIEKLIHKSLEEF
FKSATYKPLGYAVPKILDEKLEIDFNKDFEFTVVYEAYPEFEIPDISNVEVEIPEVTVSDSDVEDELKLLQLENAMVVDD
SGDVKVGSIVRVDFVELDDSLSEILTTKRQDFVFTVGESNNYYGFDNDIIGMKKDEEKIIEKNYSADYKFSELANSFKRL
KIIIKDIKKRDIPELDDDFAKDIKDSFNTLEELKAHIRENMLRLVKEKRESLKLSKLLSDVSEKLNIEIPSAMFEAELKN
VVNEFSTQNKIDLKKLNDSSMGLEGVSDVFKENVLKKLKSKLVFQKIVDNDLTEITDADLEDELVKQAEDTKMRLSEIKK
FYQEKNLLGILKDEIKRQKVKDKILKNVKEIKLKEIAFRDFINYKTGE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=446, Percent_Identity=24.8878923766816, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_BORHD (B2S0V7)

Other databases:

- EMBL:   CP000048
- RefSeq:   YP_001884033.1
- EnsemblBacteria:   EBBORT00000006863
- GeneID:   6276843
- GenomeReviews:   CP000048_GR
- KEGG:   bhr:BH0610
- GeneTree:   EBGT00050000006990
- HOGENOM:   HBG352938
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   BHER314723:BH0610-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 51805; Mature: 51805

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILNDDVKLIPGSKVEAVIKISKEFVKGKYNEILQDYSSRLKIKGFRIGKVPFSIIEGKY
CCCCCCCEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCEEECCEEECCCCHHHHCCCC
SDNIRALTIEKLIHKSLEEFFKSATYKPLGYAVPKILDEKLEIDFNKDFEFTVVYEAYPE
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
FEIPDISNVEVEIPEVTVSDSDVEDELKLLQLENAMVVDDSGDVKVGSIVRVDFVELDDS
CCCCCCCCCEEECCEEEECCCCHHHHHHHHHHCCEEEEECCCCEEECCEEEEEHHHHHHH
LSEILTTKRQDFVFTVGESNNYYGFDNDIIGMKKDEEKIIEKNYSADYKFSELANSFKRL
HHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHH
KIIIKDIKKRDIPELDDDFAKDIKDSFNTLEELKAHIRENMLRLVKEKRESLKLSKLLSD
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSEKLNIEIPSAMFEAELKNVVNEFSTQNKIDLKKLNDSSMGLEGVSDVFKENVLKKLKS
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHH
KLVFQKIVDNDLTEITDADLEDELVKQAEDTKMRLSEIKKFYQEKNLLGILKDEIKRQKV
HHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
KDKILKNVKEIKLKEIAFRDFINYKTGE
HHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MILNDDVKLIPGSKVEAVIKISKEFVKGKYNEILQDYSSRLKIKGFRIGKVPFSIIEGKY
CCCCCCCEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCEEECCEEECCCCHHHHCCCC
SDNIRALTIEKLIHKSLEEFFKSATYKPLGYAVPKILDEKLEIDFNKDFEFTVVYEAYPE
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
FEIPDISNVEVEIPEVTVSDSDVEDELKLLQLENAMVVDDSGDVKVGSIVRVDFVELDDS
CCCCCCCCCEEECCEEEECCCCHHHHHHHHHHCCEEEEECCCCEEECCEEEEEHHHHHHH
LSEILTTKRQDFVFTVGESNNYYGFDNDIIGMKKDEEKIIEKNYSADYKFSELANSFKRL
HHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHH
KIIIKDIKKRDIPELDDDFAKDIKDSFNTLEELKAHIRENMLRLVKEKRESLKLSKLLSD
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSEKLNIEIPSAMFEAELKNVVNEFSTQNKIDLKKLNDSSMGLEGVSDVFKENVLKKLKS
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHH
KLVFQKIVDNDLTEITDADLEDELVKQAEDTKMRLSEIKKFYQEKNLLGILKDEIKRQKV
HHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
KDKILKNVKEIKLKEIAFRDFINYKTGE
HHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA