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The map label for this gene is cysS

Identifier: 187918460

GI number: 187918460

Start: 630826

End: 632271

Strand: Direct

Name: cysS

Synonym: BH0599

Alternate gene names: 187918460

Gene position: 630826-632271 (Clockwise)

Preceding gene: 187918458

Following gene: 187918461

Centisome position: 68.4

GC content: 31.05

Gene sequence:

>1446_bases
ATGCTTCTTAAGTTATATAATACAAAAACAAAAAGTTTATCTGATATAAAAAATTTTAGTGATACTAAGGTTTACGCTTG
TGGTCCTACTGTTTATAATTATGCCCATATAGGTAATCTTAGAACATATATTTTTGAAGACCTGCTTATTAAGTCTTTAA
GGTTGCTAAAATATAATGTTAATTATGCAATGAATATTACTGATATTGGTCATTTAACAGGCGATTTTGATGAGGGGGAA
GATAAGGTAGTTAAAGCCGCAAGAGAGAGAGGACTTACAGTCTATGAAATTAGCAGATTTTTTACAGAAGCTTTTTTTGA
TGATTGTGCAAAATTAAATATCGTTTATCCTGATAAGGTGCTTGTTGCAAGTGAGTATATTGCTAGTATGATAGAAGTGG
TCAAAGTTCTTGAGCAAAATGGATTTACTTATTTTGTTAATGGTAATGTTTATTTTGATACGTCTCGTTTTAATGGTTAT
GGGCAGATGGCTGGTATTAATCTAAATAATTTTGGGCGTTCTTCTGTTTCTAGAGTTGAGATAGATCTTTCAAAAAAGAA
TAAATCAGACTTTGTTTTGTGGTTTACAAATTCAAAATTTAAAGATCAGGAAATGAAATGGGATTCGCCTTGGGGATTTG
GTTATCCAAGTTGGCATTTAGAATGTGCAGCAATGAATTTAGATTATTTTAAGAGTACTCTTGATATCCATTTAGGGGGA
GTTGATCATGTTGGGGTTCATCATATAAATGAAATAGCAATAGCGGAATGTTACTTAAATAAGATGTGGTGCGACATGTT
TGTTCATGGTGAATTTTTGATTATGGAAGATGAGAAAATGTCAAAATCAAATAATAATTTTATTACCATTAAAGACTTAG
AATCCGATGGATTTTCACCTTTAGATTTTAGATATTTTTGTTTAACTGCACATTATAGAACTCAACTTAAGTTTACGTTT
AGTAATCTGAGAGCTTGCAAGATAGCTAGAGAAAATATGCTTAATAAATTGACGTTTTTTTATTCTTCATTAAGTCAATT
TGACATGGCATTGCTTAATAAAAATTGTGAGAATATTGAGTCTGTTTCAGAGAATAAATACTATAATAATTTTTTGGAAA
AAATAGCTTTTGATTTAAGTATTCCTCAGGCATTAGCCTTGCTATGGGATATTGTTAAGGATGATGATCTAAGTGCTCTT
TTAAAACTTCTCCTTGCATTTAAATTTGATGAAGTTTTATCCCTTGGTTTAAAGGAAGGAGTGCTTAGAGAAATTGAGAG
GGATAGGGTAAATATTGATGATACTATGCATTCTTTGATTGAAGAGAGACGGCTTGCCAAGTTAAGAAAAGACTTTAAGC
GTGCTGATGAGATTAGAGAATATTTTCGCTCTAAGGGTTTTGTGTTAATTGATACTGAAGAAGGGACTAAGGTTAAAAGA
GGGTAG

Upstream 100 bases:

>100_bases
AATATAATAAACTTAGTATAGTAAGCTATATAGAGATTATGTTAATCGTAATTTATTTTAGTATTTTTGATCTCTAGAAT
TAATTTTTTTCACGGGATGT

Downstream 100 bases:

>100_bases
TTTTTGGCAGTATTTTTTAAGAGTCAATCTATTATTTTAAGTTTTATTTTTTTAATTTTTTTAAGTTTATTTGTTTTTTC
CGGATTTTTATTTTATTTAA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MLLKLYNTKTKSLSDIKNFSDTKVYACGPTVYNYAHIGNLRTYIFEDLLIKSLRLLKYNVNYAMNITDIGHLTGDFDEGE
DKVVKAARERGLTVYEISRFFTEAFFDDCAKLNIVYPDKVLVASEYIASMIEVVKVLEQNGFTYFVNGNVYFDTSRFNGY
GQMAGINLNNFGRSSVSRVEIDLSKKNKSDFVLWFTNSKFKDQEMKWDSPWGFGYPSWHLECAAMNLDYFKSTLDIHLGG
VDHVGVHHINEIAIAECYLNKMWCDMFVHGEFLIMEDEKMSKSNNNFITIKDLESDGFSPLDFRYFCLTAHYRTQLKFTF
SNLRACKIARENMLNKLTFFYSSLSQFDMALLNKNCENIESVSENKYYNNFLEKIAFDLSIPQALALLWDIVKDDDLSAL
LKLLLAFKFDEVLSLGLKEGVLREIERDRVNIDDTMHSLIEERRLAKLRKDFKRADEIREYFRSKGFVLIDTEEGTKVKR
G

Sequences:

>Translated_481_residues
MLLKLYNTKTKSLSDIKNFSDTKVYACGPTVYNYAHIGNLRTYIFEDLLIKSLRLLKYNVNYAMNITDIGHLTGDFDEGE
DKVVKAARERGLTVYEISRFFTEAFFDDCAKLNIVYPDKVLVASEYIASMIEVVKVLEQNGFTYFVNGNVYFDTSRFNGY
GQMAGINLNNFGRSSVSRVEIDLSKKNKSDFVLWFTNSKFKDQEMKWDSPWGFGYPSWHLECAAMNLDYFKSTLDIHLGG
VDHVGVHHINEIAIAECYLNKMWCDMFVHGEFLIMEDEKMSKSNNNFITIKDLESDGFSPLDFRYFCLTAHYRTQLKFTF
SNLRACKIARENMLNKLTFFYSSLSQFDMALLNKNCENIESVSENKYYNNFLEKIAFDLSIPQALALLWDIVKDDDLSAL
LKLLLAFKFDEVLSLGLKEGVLREIERDRVNIDDTMHSLIEERRLAKLRKDFKRADEIREYFRSKGFVLIDTEEGTKVKR
G
>Mature_481_residues
MLLKLYNTKTKSLSDIKNFSDTKVYACGPTVYNYAHIGNLRTYIFEDLLIKSLRLLKYNVNYAMNITDIGHLTGDFDEGE
DKVVKAARERGLTVYEISRFFTEAFFDDCAKLNIVYPDKVLVASEYIASMIEVVKVLEQNGFTYFVNGNVYFDTSRFNGY
GQMAGINLNNFGRSSVSRVEIDLSKKNKSDFVLWFTNSKFKDQEMKWDSPWGFGYPSWHLECAAMNLDYFKSTLDIHLGG
VDHVGVHHINEIAIAECYLNKMWCDMFVHGEFLIMEDEKMSKSNNNFITIKDLESDGFSPLDFRYFCLTAHYRTQLKFTF
SNLRACKIARENMLNKLTFFYSSLSQFDMALLNKNCENIESVSENKYYNNFLEKIAFDLSIPQALALLWDIVKDDDLSAL
LKLLLAFKFDEVLSLGLKEGVLREIERDRVNIDDTMHSLIEERRLAKLRKDFKRADEIREYFRSKGFVLIDTEEGTKVKR
G

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=329, Percent_Identity=34.9544072948328, Blast_Score=189, Evalue=7e-48,
Organism=Homo sapiens, GI62240992, Length=239, Percent_Identity=34.3096234309623, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI10835051, Length=239, Percent_Identity=34.3096234309623, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI303304987, Length=239, Percent_Identity=34.3096234309623, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI21269877, Length=239, Percent_Identity=34.3096234309623, Blast_Score=139, Evalue=6e-33,
Organism=Escherichia coli, GI1786737, Length=493, Percent_Identity=31.8458417849899, Blast_Score=224, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17509797, Length=247, Percent_Identity=34.412955465587, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17509795, Length=247, Percent_Identity=34.412955465587, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324082, Length=264, Percent_Identity=34.0909090909091, Blast_Score=147, Evalue=4e-36,
Organism=Drosophila melanogaster, GI19922218, Length=315, Percent_Identity=34.6031746031746, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI19922364, Length=253, Percent_Identity=34.7826086956522, Blast_Score=142, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_BORHD (B2S0U7)

Other databases:

- EMBL:   CP000048
- RefSeq:   YP_001884023.1
- EnsemblBacteria:   EBBORT00000006620
- GeneID:   6276869
- GenomeReviews:   CP000048_GR
- KEGG:   bhr:BH0599
- GeneTree:   EBGT00050000006936
- HOGENOM:   HBG327651
- OMA:   IGNLRTY
- ProtClustDB:   PRK14534
- BioCyc:   BHER314723:BH0599-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 55845; Mature: 55845

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 282-282

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKLYNTKTKSLSDIKNFSDTKVYACGPTVYNYAHIGNLRTYIFEDLLIKSLRLLKYNV
CEEEEECCCCCCHHHHCCCCCCEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHEEEC
NYAMNITDIGHLTGDFDEGEDKVVKAARERGLTVYEISRFFTEAFFDDCAKLNIVYPDKV
CEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHEEEEECCCHH
LVASEYIASMIEVVKVLEQNGFTYFVNGNVYFDTSRFNGYGQMAGINLNNFGRSSVSRVE
HHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCCCCEEECCCCCCCCCCCEEEE
IDLSKKNKSDFVLWFTNSKFKDQEMKWDSPWGFGYPSWHLECAAMNLDYFKSTLDIHLGG
EEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHEEEEEECC
VDHVGVHHINEIAIAECYLNKMWCDMFVHGEFLIMEDEKMSKSNNNFITIKDLESDGFSP
CCCCCHHHHHHHHHHHHHHHHHHHHHEECCEEEEEECHHCCCCCCCEEEEEECCCCCCCC
LDFRYFCLTAHYRTQLKFTFSNLRACKIARENMLNKLTFFYSSLSQFDMALLNKNCENIE
CCEEEEEEEEEEHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SVSENKYYNNFLEKIAFDLSIPQALALLWDIVKDDDLSALLKLLLAFKFDEVLSLGLKEG
HHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VLREIERDRVNIDDTMHSLIEERRLAKLRKDFKRADEIREYFRSKGFVLIDTEEGTKVKR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCC
G
C
>Mature Secondary Structure
MLLKLYNTKTKSLSDIKNFSDTKVYACGPTVYNYAHIGNLRTYIFEDLLIKSLRLLKYNV
CEEEEECCCCCCHHHHCCCCCCEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHEEEC
NYAMNITDIGHLTGDFDEGEDKVVKAARERGLTVYEISRFFTEAFFDDCAKLNIVYPDKV
CEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHEEEEECCCHH
LVASEYIASMIEVVKVLEQNGFTYFVNGNVYFDTSRFNGYGQMAGINLNNFGRSSVSRVE
HHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCCCCEEECCCCCCCCCCCEEEE
IDLSKKNKSDFVLWFTNSKFKDQEMKWDSPWGFGYPSWHLECAAMNLDYFKSTLDIHLGG
EEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHEEEEEECC
VDHVGVHHINEIAIAECYLNKMWCDMFVHGEFLIMEDEKMSKSNNNFITIKDLESDGFSP
CCCCCHHHHHHHHHHHHHHHHHHHHHEECCEEEEEECHHCCCCCCCEEEEEECCCCCCCC
LDFRYFCLTAHYRTQLKFTFSNLRACKIARENMLNKLTFFYSSLSQFDMALLNKNCENIE
CCEEEEEEEEEEHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SVSENKYYNNFLEKIAFDLSIPQALALLWDIVKDDDLSALLKLLLAFKFDEVLSLGLKEG
HHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VLREIERDRVNIDDTMHSLIEERRLAKLRKDFKRADEIREYFRSKGFVLIDTEEGTKVKR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA