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The map label for this gene is ligA

Identifier: 187918415

GI number: 187918415

Start: 570356

End: 572365

Strand: Direct

Name: ligA

Synonym: BH0552

Alternate gene names: 187918415

Gene position: 570356-572365 (Clockwise)

Preceding gene: 187918408

Following gene: 187918417

Centisome position: 61.84

GC content: 31.84

Gene sequence:

>2010_bases
ATGATCGAAGATGTAGAAGATGAAATTTTACGTTTGAAAAATATCATTAAAAGATGGAACAGAGAGTATTATGTCGATTC
TTCACCCAGTGTGGGTGATTTGACTTATGACAAAGCTCTTTTGCGACTTCAGGATTTGGAAAGTAAATATCCTGAATATA
AAACCTTGGATTCCCCCACTTTTAGGTTTGGTAGCGATCTTTTAAATGATTTTGAAGAAGTTAAACATTCTTTCCCTATA
TTAAGTTTGGATAAAACTTATGATATTAAGGGATTATCATTGTGGATTGAGAAGATGGTGCTAGAGAGTGCTATCTCAGG
ATTGCATACTGGTATTTCAGTTGAACCTAAAATTGATGGGTGTTCAATTGTTCTCCATTATAAAGATGGGATACTTGAGA
AAGCTTTGACTAGGGGAGATGGAAGGGTTGGTAATGATGTTACTGAAAATGTTAAAACAATTAGAAATGTTCCTTTGCGT
ATTGATGAAAAGATTGAATTAGTGTTGCGGGGTGAGATTTATATTACTAAGGAAAATTTTTTGAAAATAAATCGCACATT
AAAAAATCCTTATGTTAATGCTAGAAATTTAGCCTCAGGTATATTGAGAAGAATAAGTAGTAAAGAAGTTGCTAATTTTC
CTTTAGATATTTTTGTTTATGATATTTTATATTCTAGTTTGAAACTGAATACTAGTCATGATGCTTTTGACAAACTTAAG
CAATTTGGATTTAAAGTTAATCCTTTTTGTGAATTTTTTGGTGGCAAAAACTTAGAAGCAGGTATTATTGCTCATGTTAA
AAAGATAGAGGCCTTAAGAGATTCTTTTGAATATGAAATTGATGGTGTTGTGCTGAAGATTGACAGCTTTATTTTAAGGA
AAATTTTGGGATCTACTTCTCATCATCCAAAGTGGTCAATAGCTTATAAGTTTGAATCTTGCACAGGTATGAGCAAAGTG
GTAGACATAGTTGTTCAGGTTGGCCGCAGCGGGAAGATTACCCCTGTTGCACATGTAGAAAAAGTGCTTGTTGCTGGGGC
TTCTATTACTAATGCAAGTCTGCATAATCAAGATTATATAGATTTTGTTGGTTTAAATGTTGGAGATATTGTTGTGATTT
CAAGACGTGGGGATGTGATTCCTGCTGTTGACTTGGTGATAGAAAAGCTTGCGGTTGGTAGTTTTAAAATTCCTAGTAAT
TGTCCTTCGTGTAAAATGACTTTAATAAAAGAGGGTGCACATCTTTTTTGTGTAAATAGACATTGTTCTTGTCGAATAAT
TGAACAGGTAAAATATTTTTGTAGTAAGAAATGCATGAATATTGTAGGACTTTCGGAGAAAACAATAGAGTTCCTTTTTG
AAAAGAAGTTTATATCTTCAGAAGTCGAGCTTTATACTTTTAATTGTGATAGGCTTATTAATCTAAAGGGTTTTAATCTT
AAGAGAATAAATAACTTAAAGCGTTCAATTGAAGACAGTAAAAGTAGGCCGTTTAGAAAGTTGCTTCTTGGTATGGGAAT
TAAAGACCTTGGAGAGAATACAATATTAGTGCTAATTAATAACAATTTAAACTCGTTTGATACAATTAGTACTCTTTGCA
AAAATAAAGAGGCTGCTCTTGCCAAACTTTTAAAGATTAAGGGAATAGGGGAGAGGATAGCTTTAAATATTATTGAAGCA
TTTAATGATAAGACTATTCTTGATAAGTTTAATTTTTTTAAAGAATTAGGGTTGAAAATGGAGGAAGATAATACTAATGA
TGCTGTGTATGATTCCTTTTTATTTGGTAAAAAATTTTGCATTACAGGTTCTTTTAAAGGGTATTCTAGGGATGTACTTA
TTGAAAAGCTTACTAAGAAAGGTGCTGTTTTCAGCCGTTCAGTTACTAAGTGTTTAGATTTTTTACTTGTTGGAGAAAAG
TTTGGATTAAAAGTGCAAAAAGCCAATAATTTGGGTATTAAGACCTTCAGTCTTTTTGATATTAAGGATTTTGTGGATTT
AGATGATTAA

Upstream 100 bases:

>100_bases
GTTTATTACTCAAGAACTTATCCCTGAAAATTTATATTTTTATTTTCATTATTTTGTTAAAATGTAACTTGAGCTTTCAA
TTAAGTTCGATGTCAAATTT

Downstream 100 bases:

>100_bases
ATTTATTTTATTATTCTTATGTTTTTAAATTCTGATGCTAGATTTTTATATAGAGTAATTATTTCATTTGTATGCTCTAC
TTTTATTAGGTTTCTGAAAA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 669; Mature: 669

Protein sequence:

>669_residues
MIEDVEDEILRLKNIIKRWNREYYVDSSPSVGDLTYDKALLRLQDLESKYPEYKTLDSPTFRFGSDLLNDFEEVKHSFPI
LSLDKTYDIKGLSLWIEKMVLESAISGLHTGISVEPKIDGCSIVLHYKDGILEKALTRGDGRVGNDVTENVKTIRNVPLR
IDEKIELVLRGEIYITKENFLKINRTLKNPYVNARNLASGILRRISSKEVANFPLDIFVYDILYSSLKLNTSHDAFDKLK
QFGFKVNPFCEFFGGKNLEAGIIAHVKKIEALRDSFEYEIDGVVLKIDSFILRKILGSTSHHPKWSIAYKFESCTGMSKV
VDIVVQVGRSGKITPVAHVEKVLVAGASITNASLHNQDYIDFVGLNVGDIVVISRRGDVIPAVDLVIEKLAVGSFKIPSN
CPSCKMTLIKEGAHLFCVNRHCSCRIIEQVKYFCSKKCMNIVGLSEKTIEFLFEKKFISSEVELYTFNCDRLINLKGFNL
KRINNLKRSIEDSKSRPFRKLLLGMGIKDLGENTILVLINNNLNSFDTISTLCKNKEAALAKLLKIKGIGERIALNIIEA
FNDKTILDKFNFFKELGLKMEEDNTNDAVYDSFLFGKKFCITGSFKGYSRDVLIEKLTKKGAVFSRSVTKCLDFLLVGEK
FGLKVQKANNLGIKTFSLFDIKDFVDLDD

Sequences:

>Translated_669_residues
MIEDVEDEILRLKNIIKRWNREYYVDSSPSVGDLTYDKALLRLQDLESKYPEYKTLDSPTFRFGSDLLNDFEEVKHSFPI
LSLDKTYDIKGLSLWIEKMVLESAISGLHTGISVEPKIDGCSIVLHYKDGILEKALTRGDGRVGNDVTENVKTIRNVPLR
IDEKIELVLRGEIYITKENFLKINRTLKNPYVNARNLASGILRRISSKEVANFPLDIFVYDILYSSLKLNTSHDAFDKLK
QFGFKVNPFCEFFGGKNLEAGIIAHVKKIEALRDSFEYEIDGVVLKIDSFILRKILGSTSHHPKWSIAYKFESCTGMSKV
VDIVVQVGRSGKITPVAHVEKVLVAGASITNASLHNQDYIDFVGLNVGDIVVISRRGDVIPAVDLVIEKLAVGSFKIPSN
CPSCKMTLIKEGAHLFCVNRHCSCRIIEQVKYFCSKKCMNIVGLSEKTIEFLFEKKFISSEVELYTFNCDRLINLKGFNL
KRINNLKRSIEDSKSRPFRKLLLGMGIKDLGENTILVLINNNLNSFDTISTLCKNKEAALAKLLKIKGIGERIALNIIEA
FNDKTILDKFNFFKELGLKMEEDNTNDAVYDSFLFGKKFCITGSFKGYSRDVLIEKLTKKGAVFSRSVTKCLDFLLVGEK
FGLKVQKANNLGIKTFSLFDIKDFVDLDD
>Mature_669_residues
MIEDVEDEILRLKNIIKRWNREYYVDSSPSVGDLTYDKALLRLQDLESKYPEYKTLDSPTFRFGSDLLNDFEEVKHSFPI
LSLDKTYDIKGLSLWIEKMVLESAISGLHTGISVEPKIDGCSIVLHYKDGILEKALTRGDGRVGNDVTENVKTIRNVPLR
IDEKIELVLRGEIYITKENFLKINRTLKNPYVNARNLASGILRRISSKEVANFPLDIFVYDILYSSLKLNTSHDAFDKLK
QFGFKVNPFCEFFGGKNLEAGIIAHVKKIEALRDSFEYEIDGVVLKIDSFILRKILGSTSHHPKWSIAYKFESCTGMSKV
VDIVVQVGRSGKITPVAHVEKVLVAGASITNASLHNQDYIDFVGLNVGDIVVISRRGDVIPAVDLVIEKLAVGSFKIPSN
CPSCKMTLIKEGAHLFCVNRHCSCRIIEQVKYFCSKKCMNIVGLSEKTIEFLFEKKFISSEVELYTFNCDRLINLKGFNL
KRINNLKRSIEDSKSRPFRKLLLGMGIKDLGENTILVLINNNLNSFDTISTLCKNKEAALAKLLKIKGIGERIALNIIEA
FNDKTILDKFNFFKELGLKMEEDNTNDAVYDSFLFGKKFCITGSFKGYSRDVLIEKLTKKGAVFSRSVTKCLDFLLVGEK
FGLKVQKANNLGIKTFSLFDIKDFVDLDD

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigA subfamily

Homologues:

Organism=Escherichia coli, GI1788750, Length=672, Percent_Identity=32.1428571428571, Blast_Score=341, Evalue=8e-95,
Organism=Escherichia coli, GI87082305, Length=568, Percent_Identity=23.0633802816901, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_BORHD (B2S0Q2)

Other databases:

- EMBL:   CP000048
- RefSeq:   YP_001883978.1
- EnsemblBacteria:   EBBORT00000006501
- GeneID:   6277183
- GenomeReviews:   CP000048_GR
- KEGG:   bhr:BH0552
- GeneTree:   EBGT00050000007352
- HOGENOM:   HBG620317
- OMA:   HNQDYID
- ProtClustDB:   PRK14350
- BioCyc:   BHER314723:BH0552-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75702; Mature: 75702

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 117-117 BINDING 115-115 BINDING 138-138 BINDING 172-172 BINDING 286-286 BINDING 310-310

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEDVEDEILRLKNIIKRWNREYYVDSSPSVGDLTYDKALLRLQDLESKYPEYKTLDSPT
CCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
FRFGSDLLNDFEEVKHSFPILSLDKTYDIKGLSLWIEKMVLESAISGLHTGISVEPKIDG
HHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCC
CSIVLHYKDGILEKALTRGDGRVGNDVTENVKTIRNVPLRIDEKIELVLRGEIYITKENF
EEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEECCCEEEEEECEEEEEHHHH
LKINRTLKNPYVNARNLASGILRRISSKEVANFPLDIFVYDILYSSLKLNTSHDAFDKLK
EEEHHHHCCCCCCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
QFGFKVNPFCEFFGGKNLEAGIIAHVKKIEALRDSFEYEIDGVVLKIDSFILRKILGSTS
HCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEECCEEEEHHHHHHHHHHCCCC
HHPKWSIAYKFESCTGMSKVVDIVVQVGRSGKITPVAHVEKVLVAGASITNASLHNQDYI
CCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCE
DFVGLNVGDIVVISRRGDVIPAVDLVIEKLAVGSFKIPSNCPSCKMTLIKEGAHLFCVNR
EEECCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEECCCCCCCCEEHHHHCCCEEEEECC
HCSCRIIEQVKYFCSKKCMNIVGLSEKTIEFLFEKKFISSEVELYTFNCDRLINLKGFNL
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCH
KRINNLKRSIEDSKSRPFRKLLLGMGIKDLGENTILVLINNNLNSFDTISTLCKNKEAAL
HHHHHHHHHHHHHCCCCHHHHHHHCCHHHCCCCEEEEEECCCCCCHHHHHHHHCCHHHHH
AKLLKIKGIGERIALNIIEAFNDKTILDKFNFFKELGLKMEEDNTNDAVYDSFLFGKKFC
HHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHCCEEE
ITGSFKGYSRDVLIEKLTKKGAVFSRSVTKCLDFLLVGEKFGLKVQKANNLGIKTFSLFD
EECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHCEEEEECCCCCEEEEEEEC
IKDFVDLDD
HHHHCCCCC
>Mature Secondary Structure
MIEDVEDEILRLKNIIKRWNREYYVDSSPSVGDLTYDKALLRLQDLESKYPEYKTLDSPT
CCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
FRFGSDLLNDFEEVKHSFPILSLDKTYDIKGLSLWIEKMVLESAISGLHTGISVEPKIDG
HHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCC
CSIVLHYKDGILEKALTRGDGRVGNDVTENVKTIRNVPLRIDEKIELVLRGEIYITKENF
EEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEECCCEEEEEECEEEEEHHHH
LKINRTLKNPYVNARNLASGILRRISSKEVANFPLDIFVYDILYSSLKLNTSHDAFDKLK
EEEHHHHCCCCCCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
QFGFKVNPFCEFFGGKNLEAGIIAHVKKIEALRDSFEYEIDGVVLKIDSFILRKILGSTS
HCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEECCEEEEHHHHHHHHHHCCCC
HHPKWSIAYKFESCTGMSKVVDIVVQVGRSGKITPVAHVEKVLVAGASITNASLHNQDYI
CCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCE
DFVGLNVGDIVVISRRGDVIPAVDLVIEKLAVGSFKIPSNCPSCKMTLIKEGAHLFCVNR
EEECCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEECCCCCCCCEEHHHHCCCEEEEECC
HCSCRIIEQVKYFCSKKCMNIVGLSEKTIEFLFEKKFISSEVELYTFNCDRLINLKGFNL
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCH
KRINNLKRSIEDSKSRPFRKLLLGMGIKDLGENTILVLINNNLNSFDTISTLCKNKEAAL
HHHHHHHHHHHHHCCCCHHHHHHHCCHHHCCCCEEEEEECCCCCCHHHHHHHHCCHHHHH
AKLLKIKGIGERIALNIIEAFNDKTILDKFNFFKELGLKMEEDNTNDAVYDSFLFGKKFC
HHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHCCEEE
ITGSFKGYSRDVLIEKLTKKGAVFSRSVTKCLDFLLVGEKFGLKVQKANNLGIKTFSLFD
EECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHCEEEEECCCCCEEEEEEEC
IKDFVDLDD
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA