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The map label for this gene is mjaIIIM [H]

Identifier: 187918330

GI number: 187918330

Start: 490263

End: 491081

Strand: Direct

Name: mjaIIIM [H]

Synonym: BH0463A

Alternate gene names: 187918330

Gene position: 490263-491081 (Clockwise)

Preceding gene: 187918329

Following gene: 187918331

Centisome position: 53.16

GC content: 26.01

Gene sequence:

>819_bases
ATGAGCGTTGCAATACGGCCTGTTTTGAAATGGGCTGGTGGTAAGAAAAATTTATTGAAAGTTATTTTAAATAATATTCC
CCTTTCTTTTAATAATTATATTGAACCTTTTGTAGGGGGAGGGGCATTATTTTTTGCTTTGAATTTGAAAAATTCAATTA
TTAATGATATAAATTCTAATTTGATTAATTTTTATAGGGAAATTGCCTATAATTTGGATAATTTTTTATTAGAGGTTGAA
AAATATAATAATGCTCCTTTAACTAAGGAGCATTATGTTCATATTAGAAATAGTTTTAATAATGAAGATTTAACTAATTT
AGAAAAGGCATGTATTTTTCTTTATTTGAATAAAACTTGTTATAATGGTCTTTATAGAGAAAATGGAGATGGGAGATTTA
ATACTCCTTTTGGTAAATATAAAAAGATTAGTCTTTATGAAATTAAAAATTTACAATTGGCTTCTAAGCTTCTGCGGGAG
GTTAAAGTTTTAAGTCTAGATTTTTTTTGTTTACTTGATTTTATTAAAAAAGATGATTTCGTTTATCTTGATCCACCTTA
TATACCTTATTCAAAGACAAGTAATTTTACAAATTATAGTAGATATGGATTTGATGTTAGAATGCATGAAAAATTACTAC
ATTTTTGTGAGGAAATAGATAAAAAGGGGGCTAAGTTTTTGCTTTCAAATTCTAATACTGCATTTAGTCTTGGGTTATAT
AAAAACTATAATGTTGCTTTTGTTGATTCTAAAAGATTTATTAATGCAAATCCAGGTGGGCGTGGTAGTATAAGGGAAAT
TCTAGTGAAAAATTTTTAG

Upstream 100 bases:

>100_bases
TGATGCTCTTCGTGAAATACCCATTTGGTTTAAAGAAGATGAAATTTTAACTTATAAAAGGAATGATGAGCTTGAACATT
ATTATGGTTAAAATATTAAT

Downstream 100 bases:

>100_bases
TAGTATTGCATAATTGTTATAGTATTTGTGGATAGGCGTAAAGATGAGCTGTTTTAAATGGAAGCAGAGATCTTATATGG
GTTGAGTAATTTATATTATA

Product: modification methylase LlaDCHIA

Products: NA

Alternate protein names: M.MjaIII; Adenine-specific methyltransferase MjaIII [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSVAIRPVLKWAGGKKNLLKVILNNIPLSFNNYIEPFVGGGALFFALNLKNSIINDINSNLINFYREIAYNLDNFLLEVE
KYNNAPLTKEHYVHIRNSFNNEDLTNLEKACIFLYLNKTCYNGLYRENGDGRFNTPFGKYKKISLYEIKNLQLASKLLRE
VKVLSLDFFCLLDFIKKDDFVYLDPPYIPYSKTSNFTNYSRYGFDVRMHEKLLHFCEEIDKKGAKFLLSNSNTAFSLGLY
KNYNVAFVDSKRFINANPGGRGSIREILVKNF

Sequences:

>Translated_272_residues
MSVAIRPVLKWAGGKKNLLKVILNNIPLSFNNYIEPFVGGGALFFALNLKNSIINDINSNLINFYREIAYNLDNFLLEVE
KYNNAPLTKEHYVHIRNSFNNEDLTNLEKACIFLYLNKTCYNGLYRENGDGRFNTPFGKYKKISLYEIKNLQLASKLLRE
VKVLSLDFFCLLDFIKKDDFVYLDPPYIPYSKTSNFTNYSRYGFDVRMHEKLLHFCEEIDKKGAKFLLSNSNTAFSLGLY
KNYNVAFVDSKRFINANPGGRGSIREILVKNF
>Mature_271_residues
SVAIRPVLKWAGGKKNLLKVILNNIPLSFNNYIEPFVGGGALFFALNLKNSIINDINSNLINFYREIAYNLDNFLLEVEK
YNNAPLTKEHYVHIRNSFNNEDLTNLEKACIFLYLNKTCYNGLYRENGDGRFNTPFGKYKKISLYEIKNLQLASKLLREV
KVLSLDFFCLLDFIKKDDFVYLDPPYIPYSKTSNFTNYSRYGFDVRMHEKLLHFCEEIDKKGAKFLLSNSNTAFSLGLYK
NYNVAFVDSKRFINANPGGRGSIREILVKNF

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MjaIII endonuclease (Potential) [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=271, Percent_Identity=33.9483394833948, Blast_Score=135, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31528; Mature: 31396

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAIRPVLKWAGGKKNLLKVILNNIPLSFNNYIEPFVGGGALFFALNLKNSIINDINSN
CCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEEEHHHHHHHHHHHH
LINFYREIAYNLDNFLLEVEKYNNAPLTKEHYVHIRNSFNNEDLTNLEKACIFLYLNKTC
HHHHHHHHHHCHHHHEEEEECCCCCCCCHHHEEEEECCCCCCCHHHHHHEEEEEEECHHH
YNGLYRENGDGRFNTPFGKYKKISLYEIKNLQLASKLLREVKVLSLDFFCLLDFIKKDDF
HCCCEEECCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VYLDPPYIPYSKTSNFTNYSRYGFDVRMHEKLLHFCEEIDKKGAKFLLSNSNTAFSLGLY
EEECCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEE
KNYNVAFVDSKRFINANPGGRGSIREILVKNF
ECCCEEEEECCEEEECCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
SVAIRPVLKWAGGKKNLLKVILNNIPLSFNNYIEPFVGGGALFFALNLKNSIINDINSN
CCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEEEHHHHHHHHHHHH
LINFYREIAYNLDNFLLEVEKYNNAPLTKEHYVHIRNSFNNEDLTNLEKACIFLYLNKTC
HHHHHHHHHHCHHHHEEEEECCCCCCCCHHHEEEEECCCCCCCHHHHHHEEEEEEECHHH
YNGLYRENGDGRFNTPFGKYKKISLYEIKNLQLASKLLREVKVLSLDFFCLLDFIKKDDF
HCCCEEECCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VYLDPPYIPYSKTSNFTNYSRYGFDVRMHEKLLHFCEEIDKKGAKFLLSNSNTAFSLGLY
EEECCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEE
KNYNVAFVDSKRFINANPGGRGSIREILVKNF
ECCCEEEEECCEEEECCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]