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The map label for this gene is oppA [H]

Identifier: 187918201

GI number: 187918201

Start: 341237

End: 342823

Strand: Direct

Name: oppA [H]

Synonym: BH0329

Alternate gene names: 187918201

Gene position: 341237-342823 (Clockwise)

Preceding gene: 187918200

Following gene: 187918202

Centisome position: 37.0

GC content: 32.64

Gene sequence:

>1587_bases
ATGAAAATAAAAAGACATACGTTTATGATATTTTTTTTCATAGCTATTCTATCCTGCGGCAAGGAAAGTAGCAAAGAAAA
TTTGGCATTTAAAATCAGCTTAGGAGGAGAACCTAAATCAATAGATCCTCAATTATCTGAAGATAAAATGGGTGCACATG
TAATCTCGCAAATGTTTACTGGCATAGTAGATGGTGATTCACAAACTGGTGGATATAAACCAGGTCTAGCTCAATCTTGG
GACATATCTGCAGATGGAACTATATATACATTTCACTTAAGAAAAGGTCTTGTTTGGAGTGATGGGGTTCCCATTACAGC
TGAAGGTCTGCGAAAGTCTTATCTTAGAATATTAAACAAAGAAACAGCATCAAACTATGTAGGTACGATTAAGTCAACAA
TAAAAAATGCAGAAGATTACTTCGAGGGAAAAATACCGGAATCTGAACTTGGTATTAAAGCTATAGATGAATCAACACTG
GAGATATCTATTATTACTCCAAAACCTTACTTTCTAGACATGCTAGTACACCAATCTTTCACACCAGTACCAGTGCATGT
TATTGAAAAACATGGACAAGAATGGACAAAGCCTGAAAATATGGTAGTAAGTGGTGCATACAAGCTCAAGGAAAGACAAC
CCAACGAAAAAATATCACTTATAAAAAATGATAAATACTTTAATGCCAAAAACGTTGAATTACAAGAACTCATATTCTAT
ACAATAAACGATGCCTCAACTGCCTATAGAATGTATGAAAATAATGAAATAGATATGCTGACATCTGCATCAATTCCACC
TGATTTAATAAAAGAAATAAAATTAAGAAATGATTATTACTCATCCGCTATTAATGGTCTGTACTACTTTTCATTTAACA
CACACATCAAACCCCTTGATAATGTAAAGGTTAGAGAAGCTTTAACTATTGCTATTGATAGAGAAACATTAACAAAGAAA
GTTATAGACAATGGTTCTATTCCTACAAGAAACATCAGTCCAAACTTTAATAATTATTCTTACGGAAAACCACTAAGCTT
ATTTGACCCACAAAGAGCACAACAACTAATGGCTGAAGCTGGTTATCCTGATGGTATAAATTTCCCTCAACTTAAAATTA
AATATAATACAAGTGAAGGACACAAAAAAATTTCCGAATTTCTTCAAAATCAATGGTCAAAAATTTTACATATCAATGTT
GAACTTGAAAATGAAGAATGGGCATCATTCTTAGCATCTAAACAAAAGGGAGACTATGAAATCTCAAGAGCTGGTTGGCT
AGGAGATTACTCAGATCCATTAACATTCCTAAGTCTATTCCAAAAAGAGTTTTCACATTTTTCCTCATATAAATACTTTA
ATGAAGAATATGAAAATTTAATCACCCAATCTGATCTCGAGCAAGATCCAAGCAAAAGACAAGACATTTTAAGAAAAGCT
GAATCAATAATTATAGAGAAAGATTTCCCAGTCGCTCCAATATACATATATGCAGGAAATTACCTATTTAGAAATGATAA
ATGGACTGGTTGGGCACCTAACGTTGCAGAAAGATTCCATCTATCTGAACTTAAAAGAATCAAATAA

Upstream 100 bases:

>100_bases
AAATGGTTCAACAAACAAATTTTATAGGAAGAATCTTTTAGAACTTTTAGTTGTTTTTGTTGCAGTAAAAATCAACTTTA
ATAAATAGGAGATAATAAGA

Downstream 100 bases:

>100_bases
CCAATAAACAGACATCTATCAAAATATTTTAACAGATGTCTGTTTATTAATAATCCTTCAAAATTGGGTTCCATGCATTA
ATGCATAAAGTAATTAGAAA

Product: oligopeptide-binding protein OppA

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MKIKRHTFMIFFFIAILSCGKESSKENLAFKISLGGEPKSIDPQLSEDKMGAHVISQMFTGIVDGDSQTGGYKPGLAQSW
DISADGTIYTFHLRKGLVWSDGVPITAEGLRKSYLRILNKETASNYVGTIKSTIKNAEDYFEGKIPESELGIKAIDESTL
EISIITPKPYFLDMLVHQSFTPVPVHVIEKHGQEWTKPENMVVSGAYKLKERQPNEKISLIKNDKYFNAKNVELQELIFY
TINDASTAYRMYENNEIDMLTSASIPPDLIKEIKLRNDYYSSAINGLYYFSFNTHIKPLDNVKVREALTIAIDRETLTKK
VIDNGSIPTRNISPNFNNYSYGKPLSLFDPQRAQQLMAEAGYPDGINFPQLKIKYNTSEGHKKISEFLQNQWSKILHINV
ELENEEWASFLASKQKGDYEISRAGWLGDYSDPLTFLSLFQKEFSHFSSYKYFNEEYENLITQSDLEQDPSKRQDILRKA
ESIIIEKDFPVAPIYIYAGNYLFRNDKWTGWAPNVAERFHLSELKRIK

Sequences:

>Translated_528_residues
MKIKRHTFMIFFFIAILSCGKESSKENLAFKISLGGEPKSIDPQLSEDKMGAHVISQMFTGIVDGDSQTGGYKPGLAQSW
DISADGTIYTFHLRKGLVWSDGVPITAEGLRKSYLRILNKETASNYVGTIKSTIKNAEDYFEGKIPESELGIKAIDESTL
EISIITPKPYFLDMLVHQSFTPVPVHVIEKHGQEWTKPENMVVSGAYKLKERQPNEKISLIKNDKYFNAKNVELQELIFY
TINDASTAYRMYENNEIDMLTSASIPPDLIKEIKLRNDYYSSAINGLYYFSFNTHIKPLDNVKVREALTIAIDRETLTKK
VIDNGSIPTRNISPNFNNYSYGKPLSLFDPQRAQQLMAEAGYPDGINFPQLKIKYNTSEGHKKISEFLQNQWSKILHINV
ELENEEWASFLASKQKGDYEISRAGWLGDYSDPLTFLSLFQKEFSHFSSYKYFNEEYENLITQSDLEQDPSKRQDILRKA
ESIIIEKDFPVAPIYIYAGNYLFRNDKWTGWAPNVAERFHLSELKRIK
>Mature_528_residues
MKIKRHTFMIFFFIAILSCGKESSKENLAFKISLGGEPKSIDPQLSEDKMGAHVISQMFTGIVDGDSQTGGYKPGLAQSW
DISADGTIYTFHLRKGLVWSDGVPITAEGLRKSYLRILNKETASNYVGTIKSTIKNAEDYFEGKIPESELGIKAIDESTL
EISIITPKPYFLDMLVHQSFTPVPVHVIEKHGQEWTKPENMVVSGAYKLKERQPNEKISLIKNDKYFNAKNVELQELIFY
TINDASTAYRMYENNEIDMLTSASIPPDLIKEIKLRNDYYSSAINGLYYFSFNTHIKPLDNVKVREALTIAIDRETLTKK
VIDNGSIPTRNISPNFNNYSYGKPLSLFDPQRAQQLMAEAGYPDGINFPQLKIKYNTSEGHKKISEFLQNQWSKILHINV
ELENEEWASFLASKQKGDYEISRAGWLGDYSDPLTFLSLFQKEFSHFSSYKYFNEEYENLITQSDLEQDPSKRQDILRKA
ESIIIEKDFPVAPIYIYAGNYLFRNDKWTGWAPNVAERFHLSELKRIK

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=531, Percent_Identity=31.4500941619586, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI87081878, Length=479, Percent_Identity=33.6116910229645, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI1789397, Length=485, Percent_Identity=32.7835051546392, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI1789966, Length=490, Percent_Identity=26.734693877551, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1787052, Length=476, Percent_Identity=24.1596638655462, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787762, Length=493, Percent_Identity=22.7180527383367, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789887, Length=461, Percent_Identity=22.9934924078091, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1787551, Length=494, Percent_Identity=19.4331983805668, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60546; Mature: 60546

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKRHTFMIFFFIAILSCGKESSKENLAFKISLGGEPKSIDPQLSEDKMGAHVISQMFT
CCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
GIVDGDSQTGGYKPGLAQSWDISADGTIYTFHLRKGLVWSDGVPITAEGLRKSYLRILNK
HEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHH
ETASNYVGTIKSTIKNAEDYFEGKIPESELGIKAIDESTLEISIITPKPYFLDMLVHQSF
HHHHHHHHHHHHHHHCHHHHHCCCCCHHHCCCEEECCCEEEEEEECCCCHHHHHHHHCCC
TPVPVHVIEKHGQEWTKPENMVVSGAYKLKERQPNEKISLIKNDKYFNAKNVELQELIFY
CCCHHHHHHHCCCCCCCCCCEEEECCHHHHCCCCCCCEEEEECCCCCCCCCCCHHHEEEE
TINDASTAYRMYENNEIDMLTSASIPPDLIKEIKLRNDYYSSAINGLYYFSFNTHIKPLD
EECCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHCCEEEEEECCCCCCCC
NVKVREALTIAIDRETLTKKVIDNGSIPTRNISPNFNNYSYGKPLSLFDPQRAQQLMAEA
CCEEEEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
GYPDGINFPQLKIKYNTSEGHKKISEFLQNQWSKILHINVELENEEWASFLASKQKGDYE
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEEEEECCHHHHHHHHHCCCCCEE
ISRAGWLGDYSDPLTFLSLFQKEFSHFSSYKYFNEEYENLITQSDLEQDPSKRQDILRKA
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ESIIIEKDFPVAPIYIYAGNYLFRNDKWTGWAPNVAERFHLSELKRIK
HHEEEECCCCCCEEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIKRHTFMIFFFIAILSCGKESSKENLAFKISLGGEPKSIDPQLSEDKMGAHVISQMFT
CCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
GIVDGDSQTGGYKPGLAQSWDISADGTIYTFHLRKGLVWSDGVPITAEGLRKSYLRILNK
HEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHH
ETASNYVGTIKSTIKNAEDYFEGKIPESELGIKAIDESTLEISIITPKPYFLDMLVHQSF
HHHHHHHHHHHHHHHCHHHHHCCCCCHHHCCCEEECCCEEEEEEECCCCHHHHHHHHCCC
TPVPVHVIEKHGQEWTKPENMVVSGAYKLKERQPNEKISLIKNDKYFNAKNVELQELIFY
CCCHHHHHHHCCCCCCCCCCEEEECCHHHHCCCCCCCEEEEECCCCCCCCCCCHHHEEEE
TINDASTAYRMYENNEIDMLTSASIPPDLIKEIKLRNDYYSSAINGLYYFSFNTHIKPLD
EECCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHCCEEEEEECCCCCCCC
NVKVREALTIAIDRETLTKKVIDNGSIPTRNISPNFNNYSYGKPLSLFDPQRAQQLMAEA
CCEEEEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
GYPDGINFPQLKIKYNTSEGHKKISEFLQNQWSKILHINVELENEEWASFLASKQKGDYE
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEEEEECCHHHHHHHHHCCCCCEE
ISRAGWLGDYSDPLTFLSLFQKEFSHFSSYKYFNEEYENLITQSDLEQDPSKRQDILRKA
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ESIIIEKDFPVAPIYIYAGNYLFRNDKWTGWAPNVAERFHLSELKRIK
HHEEEECCCCCCEEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]