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The map label for this gene is wcaM

Identifier: 187733294

GI number: 187733294

Start: 1094366

End: 1095760

Strand: Direct

Name: wcaM

Synonym: SbBS512_E1189

Alternate gene names: 187733294

Gene position: 1094366-1095760 (Clockwise)

Preceding gene: 187730685

Following gene: 187733487

Centisome position: 23.71

GC content: 47.46

Gene sequence:

>1395_bases
ATGCCATTTAAAAAACTCTCCCGACGTACCTTCCTGACGGCAAGCTCGGCGCTTGCCTTCCTCCATACCCCTTTCGCCCG
CGCACTTCCCGCCCGACAAAGCGTTAACATTAACGACTACAACCTACACGACTGGATCGCCTCATTTAAACAAGCCTTCG
GCGAAGGGCAAACGGTCGTCGTGCCTGCCGGATTCGTTTGTGACAATATCAACGCCGGCATCTTCATTCCTCCTGGCAAA
ACGTTACACATCCTCGGAAGCCTGCGAGGCAACGGCAGAGGGCGATTTGTCTTACAGGACGGCAGCCAGGTGACAGGGGA
GGAGGGCGGCAGTATGCATAACATCACCCTGGATGTGCGTGGCTCTGACTGCACCATCAAAGGGCTGGCGATGAGCGGCT
TTGGCCCGGTAACGCAGATTTATATCGGCGGCAAAAACAAACGGGTCATGCGCAACCTGACCATCGATAACCTCACTGTC
AGCCACGCTAATTACGCCATCTTACGCCAGGGATTTCATAACCAGATTATCGGTGCCAACATCACCAATTGTAAGTTCAG
CGACTTACAGGGCGATGCCATTGAATGGAACGTGGCAATTAACGACAGTGATATTTTGATCTCCGACCACATCATCGAGC
GCATCAACTGTACTAACGGAAAAATCAACTGGGGCATTGGCATAGGTCTTGCGGGAAGCACTTATGATAATAATTACCCG
GAAGACCAGTCAGTGAAAAACTTTGTCGTGGCGAATATCACGGGATCGGATTGTCGGCAGTTGATCCATGTTGAAAATGG
TAAACATTTTGTTATTCGTAATATCAAAGCCCGCAATATCACGCCGGATTTCAGTAAGAAAGCAGGCATTGATAACGCGA
CAGTTGCTATTTACGGTTGTGACAATTTCGTGATTGATAATATTGAAATGATTAATAGTGCCGGGATGTTAATCGGCTAT
GGGGTAATTAAAGGCAAATATCTCTCGATACCGCAAAATTTCCAAGTGAATAATATTCAACTGGATAACACCCATCTTGC
TTATAAATTGCGCGGCATCCAAATCTCCGCCGGGAATGCTGTCTCCTTTGTGGCGCTGACTAACATTGAGATGAAGCGTG
CGTCGCTGGAGTTACACAACAAACCGCAACATCTTTTTATGCGTAATATCAAGGTGATGCAGGAATCCTCAGTTGGACCA
GCATTGAGCATGAACTTCGACATGCGCAAAGACGTTCGCGGCGTCTTTATGGCGAAAAAAGAAACACTGCTGTCTCTTGC
AAATGTTCATGCGGTGAATGAAAAAGGGCAAAGCTCCGTCGATATCGACAGAGTTAATCACCATATTGTTAATGTGGAAA
AGATTAACTTTAGATTGCCGGAACGGAGAGAGTAG

Upstream 100 bases:

>100_bases
GTCGTCAAACGCGCGCGCGAAAAAGTCGAACACGATTTTAACCAGCAGGTGATTAATCGAGAACTCGCCAGCTTGTTGCA
GGCTTTATAGAGGAGAACGC

Downstream 100 bases:

>100_bases
ATTTGCGACCATTCATAAACTTACTGGATATAATGTTCTAAAAGATTATAATCCTCACACTAACTGTTAAAGCTGGGGCT
GGGCAGCATTGACTACTCAG

Product: putative colanic acid biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNLHDWIASFKQAFGEGQTVVVPAGFVCDNINAGIFIPPGK
TLHILGSLRGNGRGRFVLQDGSQVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHIIERINCTNGKINWGIGIGLAGSTYDNNYP
EDQSVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGY
GVIKGKYLSIPQNFQVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKVMQESSVGP
ALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIVNVEKINFRLPERRE

Sequences:

>Translated_464_residues
MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNLHDWIASFKQAFGEGQTVVVPAGFVCDNINAGIFIPPGK
TLHILGSLRGNGRGRFVLQDGSQVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHIIERINCTNGKINWGIGIGLAGSTYDNNYP
EDQSVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGY
GVIKGKYLSIPQNFQVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKVMQESSVGP
ALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIVNVEKINFRLPERRE
>Mature_463_residues
PFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNLHDWIASFKQAFGEGQTVVVPAGFVCDNINAGIFIPPGKT
LHILGSLRGNGRGRFVLQDGSQVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTVS
HANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHIIERINCTNGKINWGIGIGLAGSTYDNNYPE
DQSVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYG
VIKGKYLSIPQNFQVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKVMQESSVGPA
LSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIVNVEKINFRLPERRE

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788356, Length=464, Percent_Identity=96.551724137931, Blast_Score=926, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011050 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 51219; Mature: 51088

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNLHDWIASFKQAFGEGQTVV
CCHHHHCCEEEEEHHHHHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
VPAGFVCDNINAGIFIPPGKTLHILGSLRGNGRGRFVLQDGSQVTGEEGGSMHNITLDVR
EECCEEECCCCCCEEECCCCEEEEEEECCCCCCEEEEEECCCEECCCCCCCEEEEEEEEE
GSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTVSHANYAILRQGFHNQIIGAN
CCCEEEEEEEECCCCCEEEEEECCCCCEEEEEEEECEEEECCCCHHHHHCCCCCCCCCCC
ITNCKFSDLQGDAIEWNVAINDSDILISDHIIERINCTNGKINWGIGIGLAGSTYDNNYP
CCCCEECCCCCCEEEEEEEECCCCEEEECHHHHHEECCCCEEEEEEEEEECCCCCCCCCC
EDQSVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGC
CCCCCCEEEEEECCCHHHHEEEEECCCCEEEEEEEEECCCCCCCHHHCCCCCCEEEEEEC
DNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNFQVNNIQLDNTHLAYKLRGIQISAGNA
CCEEECCEEEECCCCEEEEEEEECCEEECCCCCCEEEEEEECCCEEEEEEEEEEEECCCE
VSFVALTNIEMKRASLELHNKPQHLFMRNIKVMQESSVGPALSMNFDMRKDVRGVFMAKK
EEEEEEECEEEEEEEEEECCCCHHHHHHHHHHEECCCCCCEEEECCCHHHCCHHEEEEHH
ETLLSLANVHAVNEKGQSSVDIDRVNHHIVNVEKINFRLPERRE
HHHHHHHHEEEECCCCCCCCCHHHCCEEEEEEEEEEEECCCCCC
>Mature Secondary Structure 
PFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNLHDWIASFKQAFGEGQTVV
CHHHHCCEEEEEHHHHHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
VPAGFVCDNINAGIFIPPGKTLHILGSLRGNGRGRFVLQDGSQVTGEEGGSMHNITLDVR
EECCEEECCCCCCEEECCCCEEEEEEECCCCCCEEEEEECCCEECCCCCCCEEEEEEEEE
GSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTVSHANYAILRQGFHNQIIGAN
CCCEEEEEEEECCCCCEEEEEECCCCCEEEEEEEECEEEECCCCHHHHHCCCCCCCCCCC
ITNCKFSDLQGDAIEWNVAINDSDILISDHIIERINCTNGKINWGIGIGLAGSTYDNNYP
CCCCEECCCCCCEEEEEEEECCCCEEEECHHHHHEECCCCEEEEEEEEEECCCCCCCCCC
EDQSVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGC
CCCCCCEEEEEECCCHHHHEEEEECCCCEEEEEEEEECCCCCCCHHHCCCCCCEEEEEEC
DNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNFQVNNIQLDNTHLAYKLRGIQISAGNA
CCEEECCEEEECCCCEEEEEEEECCEEECCCCCCEEEEEEECCCEEEEEEEEEEEECCCE
VSFVALTNIEMKRASLELHNKPQHLFMRNIKVMQESSVGPALSMNFDMRKDVRGVFMAKK
EEEEEEECEEEEEEEEEECCCCHHHHHHHHHHEECCCCCCEEEECCCHHHCCHHEEEEHH
ETLLSLANVHAVNEKGQSSVDIDRVNHHIVNVEKINFRLPERRE
HHHHHHHHEEEECCCCCCCCCHHHCCEEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]