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The map label for this gene is gne

Identifier: 187733487

GI number: 187733487

Start: 1095918

End: 1096913

Strand: Direct

Name: gne

Synonym: SbBS512_E1190

Alternate gene names: 187733487

Gene position: 1095918-1096913 (Clockwise)

Preceding gene: 187733294

Following gene: 187732350

Centisome position: 23.74

GC content: 45.28

Gene sequence:

>996_bases
ATGAACGATAACGTTTTGCTCATAGGAGCTTCCGGATTCGTAGGAACCCGACTACTTGAAACGGCAATTGCTGACTTTAA
TATCAAGAACCTGGACAAACAGCAGAGCCATTTTTATCCAGCAATCACACAGATTGGCGATGTTCGTGATCAACAGGCAC
TCGACCAGGCGTTAGCCGGTTTTGACACTGTTGTACTACTGGCAGCGGAACACCGCGATGACGTCAGCCCTACTTCTCTC
TATTATGATGTCAACGTTCAGGGTACCCGCAATGTGCTGGCGGCCATGGAAAAAAATGGCGTTAAAAATATCATCTTTAC
CAGTTCCGTTGCTGTTTATGGTTTGAACAAACACAACCCTGACGAAAACCATCCACACGACCCTTTCAACCACTACGGCA
AAAGTAAGTGGCAGGCAGAGGAAGTGCTGCGTGAATGGTATAACAAAGCACCAACAGAACGTTCATTAACCATCATCCGT
CCTACCGTTATCTTCGGTGAACGCAACCGCGGTAACGTCTATAACTTGCTGAAACAGATCGCTGGCGGCAAGTTTATGAT
GGTGGGCGCAGGGACTAACTATAAGTCCATGGCTTATGTTGGAAACATTGTTGAGTTTATCAAGTACAAACTGAAGAATG
TTGCCGCAGGTTATGAGGTTTATAACTATGTTGATAAGCCAGACCTGAACATGAACCAGTTGGTTGCTGAAGTTGAACAA
AGCCTGAACAAAAAGATCCCTTCTATGCACTTGCCTTACCCACTAGGAATGCTGGGTGGATATTGCTTTGATATCCTGAG
CAAAATTACGGGCAAAAAATACGCTGTCAGCTCTGTGCGCGTGAAAAAATTCTGCGCAACAACACAGTTTGACGCAACGA
AAGTGCATTCTTCAGGTTTTGTGGCACCGTATACGCTGTCGCAAGGTCTGGATCGAACTCTGCAGTATGAATTCGTCCAT
GCCAAAAAAGACGACATAACGTTTGTTTCTGAGTAA

Upstream 100 bases:

>100_bases
CACTAACTGTTAAAGCTGGGGCTGGGCAGCATTGACTACTCAGGGAAGGTACTCATCTTTATCTTGTGTTTCTTACGTAA
CAACCAGACTAAAGTGATTT

Downstream 100 bases:

>100_bases
TGCAGACGAGTCAGAGAAAAAAGGACCAGGTTGGACTACGATTATTCGGAAAGAGGTACTGATTAAATTATCAGTATGCG
GAATTCCGAGTAATCCGAGT

Product: UDP-N-acetylglucosamine 4-epimerase

Products: NA

Alternate protein names: UDP-GlcNAc 4-epimerase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPAITQIGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSL
YYDVNVQGTRNVLAAMEKNGVKNIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGYEVYNYVDKPDLNMNQLVAEVEQ
SLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAVSSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVH
AKKDDITFVSE

Sequences:

>Translated_331_residues
MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPAITQIGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSL
YYDVNVQGTRNVLAAMEKNGVKNIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGYEVYNYVDKPDLNMNQLVAEVEQ
SLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAVSSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVH
AKKDDITFVSE
>Mature_331_residues
MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPAITQIGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSL
YYDVNVQGTRNVLAAMEKNGVKNIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGYEVYNYVDKPDLNMNQLVAEVEQ
SLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAVSSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVH
AKKDDITFVSE

Specific function: Converts UDP-GlcNAc to UDP-GalNAc. Essential for O antigen synthesis [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=262, Percent_Identity=28.2442748091603, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI8393516, Length=262, Percent_Identity=28.2442748091603, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI56237023, Length=294, Percent_Identity=27.5510204081633, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI56118217, Length=294, Percent_Identity=27.5510204081633, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI189083684, Length=294, Percent_Identity=27.5510204081633, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1786974, Length=299, Percent_Identity=30.4347826086957, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081792, Length=306, Percent_Identity=24.8366013071895, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321437, Length=345, Percent_Identity=26.3768115942029, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.7 [H]

Molecular weight: Translated: 37085; Mature: 37085

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPAITQIGDVRDQQALDQALAG
CCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHCCHHHHHHHHHHHC
FDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVKNIIFTSSVAVYGLNKHNP
CCEEEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEECCEEEEECCCCCC
DENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIRPTVIFGERNRGNVYNLLKQI
CCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHH
AGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGYEVYNYVDKPDLNMNQLVAEVEQ
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
SLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAVSSVRVKKFCATTQFDATKVHSSGF
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCHHEECCCCC
VAPYTLSQGLDRTLQYEFVHAKKDDITFVSE
CCCHHHHHCCCHHEEEEEEECCCCCEEEECC
>Mature Secondary Structure
MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPAITQIGDVRDQQALDQALAG
CCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHCCHHHHHHHHHHHC
FDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVKNIIFTSSVAVYGLNKHNP
CCEEEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEECCEEEEECCCCCC
DENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIRPTVIFGERNRGNVYNLLKQI
CCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHH
AGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGYEVYNYVDKPDLNMNQLVAEVEQ
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
SLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAVSSVRVKKFCATTQFDATKVHSSGF
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCHHEECCCCC
VAPYTLSQGLDRTLQYEFVHAKKDDITFVSE
CCCHHHHHCCCHHEEEEEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11976290; 11206551; 11258796 [H]