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The map label for this gene is insB1 [H]

Identifier: 187731671

GI number: 187731671

Start: 979634

End: 980011

Strand: Direct

Name: insB1 [H]

Synonym: SbBS512_E1074

Alternate gene names: 187731671

Gene position: 979634-980011 (Clockwise)

Preceding gene: 187730730

Following gene: 187733146

Centisome position: 21.22

GC content: 53.44

Gene sequence:

>378_bases
ATGGACGAACAGTGGGGATACGTCGGGGCTAAATCGCGCCAGCGCTGGCTGTTTTACGCGTATGACAGGCTCCGGAAGAC
GGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGGGCGTCTTATGAGCTTGCTGTCACCCTTTGACGTGG
TGATATGGATGACGGATGGCTGGCCACTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACG
CAGCGAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATC
GGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAA

Upstream 100 bases:

>100_bases
GCGTTAGCCTCAACACGATTTTACGTCACTTAAAAAACTCAGGCCGCAGTCGGTAACCTCGCGCATACAGCCGGGCAGTG
ACGTCATCGTCTGCGCGGAA

Downstream 100 bases:

>100_bases
GTTGGAGTCATTACCATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTC
AGGCTGAATAAGATTACTCT

Product: IS1 transposase orfB

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYT
QRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Sequences:

>Translated_125_residues
MDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYT
QRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
>Mature_125_residues
MDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYT
QRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=125, Percent_Identity=99.2, Blast_Score=256, Evalue=4e-70,
Organism=Escherichia coli, GI1788203, Length=125, Percent_Identity=99.2, Blast_Score=256, Evalue=4e-70,
Organism=Escherichia coli, GI1786203, Length=125, Percent_Identity=99.2, Blast_Score=256, Evalue=4e-70,
Organism=Escherichia coli, GI1787223, Length=125, Percent_Identity=99.2, Blast_Score=256, Evalue=4e-70,
Organism=Escherichia coli, GI1786470, Length=125, Percent_Identity=99.2, Blast_Score=255, Evalue=5e-70,
Organism=Escherichia coli, GI1786459, Length=125, Percent_Identity=99.2, Blast_Score=255, Evalue=5e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 14931; Mature: 14931

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECC
WPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLN
CCHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHC
IKHYQ
CCCCC
>Mature Secondary Structure
MDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECC
WPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLN
CCHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHC
IKHYQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]